Many intracellular microbial symbionts of arthropods are strictly vertically transmitted and manipulate their host's reproduction in ways that enhance their own transmission. Rare horizontal transmission events are nonetheless necessary for symbiont spread to novel host lineages. Horizontal transmission has been mostly inferred from phylogenetic studies but the mechanisms of spread are still largely a mystery. Here, we investigated transmission of two distantly related bacterial symbionts – Rickettsia and Hamiltonella – from their host, the sweet potato whitefly, Bemisia tabaci, to three species of whitefly parasitoids: Eretmocerus emiratus, Eretmocerus eremicus and Encarsia pergandiella. We also examined the potential for vertical transmission of these whitefly symbionts between parasitoid generations. Using florescence in situ hybridization (FISH) and transmission electron microscopy we found that Rickettsia invades Eretmocerus larvae during development in a Rickettsia-infected host, persists in adults and in females, reaches the ovaries. However, Rickettsia does not appear to penetrate the oocytes, but instead is localized in the follicular epithelial cells only. Consequently, Rickettsia is not vertically transmitted in Eretmocerus wasps, a result supported by diagnostic polymerase chain reaction (PCR). In contrast, Rickettsia proved to be merely transient in the digestive tract of Encarsia and was excreted with the meconia before wasp pupation. Adults of all three parasitoid species frequently acquired Rickettsia via contact with infected whiteflies, most likely by feeding on the host hemolymph (host feeding), but the rate of infection declined sharply within a few days of wasps being removed from infected whiteflies. In contrast with Rickettsia, Hamiltonella did not establish in any of the parasitoids tested, and none of the parasitoids acquired Hamiltonella by host feeding. This study demonstrates potential routes and barriers to horizontal transmission of symbionts across trophic levels. The possible mechanisms that lead to the differences in transmission of species of symbionts among species of hosts are discussed.
Citation: Chiel E, Zchori-Fein E, Inbar M, Gottlieb Y, Adachi-Hagimori T, Kelly SE, et al. (2009) Almost There: Transmission Routes of Bacterial Symbionts between Trophic Levels. PLoS ONE 4(3): e4767. https://doi.org/10.1371/journal.pone.0004767
Editor: Jason E. Stajich, University of California, Berkeley, United States of America
Received: December 4, 2008; Accepted: February 10, 2009; Published: March 10, 2009
Copyright: © 2009 Chiel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This research was supported by the following sources: grant No 2004416 from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel to EZ-F and MSH; The National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant No 2006-35302-17165 to MSH and EZ-F and The United-States - Israel Binational Agricultural Research and Development fund (BARD), Graduate Student Fellow award No GS-1-2007 to EC. Contribution No 503/08 from the Agricultural Research Organization, Bet Dagan, Israel. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The occurrence of arthropods serving as hosts for bacterial symbionts is very common. Primary, obligate symbionts that provide essential nutrients lacking in the host's diet, are strictly maternally transmitted and show congruent phylogenies with those of their host group , . Facultative, secondary symbionts are also transmitted vertically, and promote their own transmission by contributing to host fitness or by manipulating the host's reproduction –. Phylogenetic trees of secondary symbionts are largely incongruent with those of their hosts. This, and the fact that the same secondary symbionts are sometimes found in distantly related hosts, is attributed to rare horizontal transmission events of the symbionts between species , , .
The routes of horizontal transmission are not very well known, although transmission via common host plants and/or common natural enemies has been hypothesized, and phylogenetic evidence for the latter has been provided –. Rare examples of experimentally demonstrated natural intra-specific horizontal transmission include Arsenophonus , Wolbachia  and a virus  in parasitoids, as well as transmission between mates of the same aphid species . In contrast, documentation of inter-specific transmission is almost non-existent. Huigens et al  showed horizontal transmission of Wolbachia between conspecifics of Trichogramma kayaki when developing within the same host. However, attempts to show inter-specific horizontal transmission of Wolbachia by the same mechanism, between Trichogramma species, resulted in loss of the symbiont from the recipient species within a few generations . In lieu of more natural examples, some microinjection studies have been successful in establishing some new stable associations –, yet others have been unsuccessful in establishing novel symbiont-host associations –, suggesting limits to the ability of symbionts to colonize the germ line of some hosts. While elegant work has shown how Wolbachia colonizes the germ line of a Drosophila host following injection of cured individuals , why symbionts fail to become established is not understood.
The intimate interaction between hosts and their endo-parasitoids would seem to provide opportunities for horizontal transmission of symbionts, as parasitoid larvae consume nothing but symbiont-contaminated food throughout their development. Yet, to our knowledge, there is no experimental evidence of permanent acquisition of arthropods' symbionts by their natural enemies, hence the notion that inter-specific horizontal transmission is a rare event.
Here we followed transmission routes of symbionts from their host – the sweet potato whitefly, Bemisia tabaci – to parasitoids. Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a minute insect that feeds on phloem sap of numerous host plants and is a major pest of agricultural crops . Bemisia tabaci harbors a primary symbiont, Portiera aleyrodidarum that most probably produces amino acids lacking in the phloem diet . This primary symbiont is located only within specialized cells – bacteriocytes – that are aggregated in two clusters called bacteriomes . In addition, B. tabaci may harbor a variety of secondary symbionts: Arsenophonus, Cardinium, Fritschea, Hamiltonella, Rickettsia and Wolbachia (reviewed in , ; ), whose function is yet mostly unknown. The B. tabaci colony used in our study carried only two of those secondary symbionts: Hamiltonella and Rickettsia. Hamiltonella is located inside the bacteriocytes with the primary symbiont, while the Rickettsia in our culture is dispersed throughout the hemocoel .
Bacteria of the genus Rickettsia (α-Proteobacteria) are best known as vector-borne agents of many vertebrate diseases. The more recent discoveries of Rickettsia in many different invertebrates, with diverse effects such as reproductive manipulation, heat tolerance and plant disease, suggest the disease-causing members represent a small portion of a much larger group . The Rickettsia in B. tabaci is most closely related to the pea aphid Rickettsia, is found in all developmental stages of the whitefly, and is maternally transmitted . Rickettsia is highly prevalent in B. tabaci populations , but its benefits to the host, if any, are not clear. As a matter of fact, Rickettsia was found to inflict some costs on fitness parameters of B. tabaci , . Hamiltonella (γ-Proteobacteria) was described from the pea aphid, Acyrthosiphon pisum, where it occurs in various tissues both extra- and intra- cellularly and benefits its host by conferring resistance against parasitoids , , .
Bemisia tabaci is attacked by a wide variety of natural enemies, including parasitoids of the genera Eretmocerus and Encarsia (Hymenoptera: Aphelinidae) . These two genera belong to two different sub-families: Eretmocerus, with 16 species recorded from B. tabaci , is in the Aphelininae subfamily; Encarsia, with 344 described species, of which 175 species attack whiteflies, is in the Coccophaginae subfamily . Eretmocerus and Encarsia also differ markedly in their mode of development: Eretmocerus spp. lay a single egg under the host venter (i.e., between the host and leaf) and the first instar penetrates and develops within a vital cellular capsule inside the host . Encarsia spp., in contrast, lay the egg directly into the body of their whitefly host .
In preliminary screening we found that two species of Eretmocerus, Er. eremicus (Rose & Zolnerowich) and Er. sp. nr. emiratus (Zolnerowich & Rose), were both highly infected with a Rickettsia that had the same 16S rDNA and citrate synthase gene sequences as the Rickettsia in their host, B. tabaci. Therefore the current study was initiated to address two key questions:
- What (if any) are the routes of transmission of Rickettsia and Hamiltonella from B. tabaci to the whitefly's parasitoids?
- Are symbionts that are acquired by the parasitoids then vertically transmitted to parasitoid offspring?
Materials and Methods
Two B. tabaci (biotype B) colonies were used for the study: one that carried Rickettsia (R+) and one that did not (R−). Rickettsia in these whiteflies was distributed throughout the hemocoel, the ‘scattered’ phenotype . The presence/absence of Rickettsia was routinely monitored by diagnostic PCR, as described below. Additionally, the secondary symbiont Hamiltonella was established in all individuals of both colonies. Each colony was reared in a separate room at 27±1°C, ca. 60% RH and 16∶8 L∶D. Both colonies have been maintained for over two years on cowpea plants (Vigna unguiculata var. California blackeye).
Eretmocerus sp. nr. emiratus, Er. eremicus and Encarsia pergandiella were each reared separately on cowpea plants that were infested with R+ B. tabaci nymphs as hosts, inside transparent ventilated plastic jars. Both sexes of Eretmocerus spp. develop as solitary, primary parasitoids, whereas Encarsia pergandiella is an autoparasitoid ; females are primary parasitoids of whiteflies and males are hyperparasitic, developing on conspecific or heterospecific immatures. Male En. pergandiella were thus produced by exposing Er. eremicus larvae and pupae to adult female En. pergandiella. All parasitoid cultures were kept in a climate-controlled walk-in chamber (27±1°C, ca. 60% RH and 16∶8 L∶D).
3. Establishment of symbiont-free parasitoid colonies.
Eretmocerus emiratus and Er. eremicus were fed on honey containing 50 mg/ml Rifampicin for 48 hrs and were then released on cowpea plants bearing R− B. tabaci nymphs for oviposition. This process was repeated for two consecutive generations. The infection status of the progeny was then checked with PCR and both species were found to be free of Rickettsia and Hamiltonella, therefore they were continuously reared on R− whiteflies under the conditions described above. Encarsia pergandiella was not treated the same way because neither Rickettsia nor Hamiltonella were detected in adult wasps after development in infected whiteflies.
4. PCR analysis.
To extract DNA, individual whiteflies or wasps were ground in a 3 µl droplet of proteinase K solution (20 mg/ml, Invitrogen). The droplet was then transferred into a tube containing 50 µl of sterile 10% Chelex beads (Sigma-Aldrich) in PCR water. The tubes were incubated at 37°C for 1 h, then at 96°C for 8 min and then kept at −20°C until analysis. Two microliters of the DNA lysate were used as a template for PCR reactions. The presence of Rickettsia was determined using specific primers for amplifying 16S rDNA gene fragments: 528F [5-ACTAATCTAGAGTGTAGTAGGGGATGATGG-3] and 1044R [5-GTTTTCTTATAGTTCCTGGCATTACCC-3]. PCR conditions were: 95°C for 2 min followed by 35 cycles of 92°C, 30 s; 60°C, 30 s; 72°C, 30 s, and final incubation at 72°C for 5 min. Screening for other B. tabaci symbionts, including Hamiltonella, was done using the primers and conditions described in . Reactions were carried in a 10 µl volume containing 4 pmol of each primer, 0.01 µmol dNTP's, 1× “Thermopol” buffer and 0.4 units of Taq DNA polymerase (New England Biolabs). PCR products were visualized on 1.5% agarose gel using SYBR-Green (Cambrex Bio Science Rockland Inc.). To verify the identity of the PCR products, bands were eluted, DNA was purified (QIAquick gel purification kit, Qiagen) and sent for direct sequencing at the University of Arizona's sequencing facility. The resulting sequences were compared to known sequences using the BLAST algorithm in NCBI. Sequences from whiteflies and parasitoids were compared to one another using the BLAST 2 Sequence in NCBI.
5. Visualization of Rickettsia using Fluorescence In Situ Hybridization (FISH) and Transmission Electron Microscopy (TEM).
FISH of B. tabaci parasitized nymphs, and adult parasitoids was performed with Rickettsia-specific 16S rRNA DNA probes, as described in . Stained samples were whole mounted and viewed under an IX81Olympus FluoView™500 confocal microscope (Tokyo, Japan). Reproducibility and controls were performed as described in the above reference (at least 20 individuals of each species). Samples of Er. eremicus females for TEM were prepared as described by  (n = 5 females).
Experiments and Experimental design
6. Acquisition and maintenance of Rickettsia and Hamiltonella in whitefly parasitoids.
Wasps that developed on Rickettsia- and Hamiltonella-infected whiteflies were censused for infection. Using a fine needle, approx. 100 pupae of each wasp species were removed from leaves and placed in a glass vial with honey. Samples of the pupae were placed in 96% ethanol for diagnostic PCR. Newly emerged wasps were transferred to a new vial with honey and samples were placed in 96% ethanol. Subsequently, wasps were sampled and placed in ethanol on days 3, 6, 9 and 12 post-eclosion. Infection status was then determined by diagnostic PCR in 10–13 wasps of each species, at each time point.
7. Transmission of symbionts from B. tabaci to parasitoids.
There are three likely routes by which symbionts can be transmitted from the whitefly host to its parasitoids: 1) the parasitoid larva acquires symbionts while feeding and developing in an infected host; 2) the adult female wasps acquire symbionts via host-feeding (piercing of the whitefly integument with the ovipositor followed by consumption of host hemolymph); 3) adult wasps might acquire the symbiont via feeding on honeydew secretions of infected whitefly hosts. To test these pathways, cowpea leaf disks (30 mm diameter) infested with 30–50 R+ B. tabaci nymphs (2nd and 3rd instars) were placed on 1% agar inside 35 mm Petri dishes and sealed with screen lids. One male and one female of R− wasps (cured Er. emiratus and Er. eremicus grown for six generations on R− whiteflies or R− En. pergandiella directly from the culture) were introduced onto each leaf disk for 24 hrs and were then collected to 96% ethanol for PCR analysis. The percentage of infection status of these adults was used to determine the acquisition of the symbiont via either host-feeding or feeding on infected honeydew (scenarios 2 and 3 above). For controls, wasps from the same sources were introduced onto leaf disks bearing R− whiteflies, and some wasps were placed directly in ethanol, without exposure to hosts. The leaf disks bearing parasitized whiteflies were then incubated for approximately two weeks until wasp progeny emergence and then two to five (at least one male and one female) wasps from each disk were collected and placed in 96% ethanol. An estimate of the percentage of symbiont acquisition via exposure during development (scenario 1 above) was determined by the infection status of this second group of wasps. Results were subjected to a chi-square test (JMP 6.1 software, SAS Institute). Figure 1 illustrates the set up of this experiment, as well as the vertical transmission experiment (#8, below).
Infection status is indicated either by red “+” sign or blue “−” sign. R = Rickettsia, H = Hamiltonella. TRT = treatment. Whitefly hosts are illustrated as small yellow ovals on the (green) leaf disks. To test transmission of symbionts from B. tabaci to parasitoids, one female parasitoid was introduced to each leaf disk for 24 h, after which they were tested by PCR. From the emerging F1, one or two females from each replicate were used to continue to the vertical transmission experiment, while the rest of the cohort was tested by PCR (two-five from each cohort). The emerging F2 were all collected and two-five from each cohort were tested by PCR.
To study whether symbiont acquisition via host feeding was permanent or transient, another experiment was carried out. Here, approximately 50 R− wasps were introduced onto a plant infested with R+ B. tabaci nymphs (each species on a separate plant). After 24 h the wasps were retrieved, half of them were transferred directly to 96% ethanol and the other half were kept in glass vials with honey for four days, and then also placed in ethanol. Twenty wasps of each species were screened for Rickettsia by PCR: ten wasps from the half that were transferred to ethanol immediately after the exposure to R+ whiteflies, and ten from the half that were fed on honey after exposure.
8. Vertical transmission experiments.
To study if Rickettsia and Hamiltonella are vertically transmitted between parasitoid generations, cowpea leaf disks bearing R− B. tabaci nymphs were prepared and parasitoids of three treatments were randomly assigned to them: 1) F1adults from the previous transmission experiment that were exposed to R+ hosts during development, i.e. wasps that have been exposed to the symbionts for one generation only; 2) wasps that had been reared on R+ hosts for many generations; 3) adults from the horizontal transmission experiment that emerged from R− hosts (control). One female parasitoid was introduced onto each leaf disk for 24 hrs and was then placed in 96% ethanol for PCR analysis. The leaf disks were incubated for approximately two weeks until progeny emergence and then two to five (at least one male and one female) progeny from each disk were collected and placed in 96% ethanol for PCR analysis. The set up of this experiment is illustrated in Fig. 1.
Acquisition and maintenance of Rickettsia and Hamiltonella in whitefly parasitoids
Almost all pupae of the three studied species carried Rickettsia and Hamiltonella (Er. emiratus- 11 out of 12 infected; Er. eremicus – 13/13; En. pergandiella females - 10/10; En. pergandiella males- 10/10). However, infection of adult wasps differed significantly between the two genera of wasps: Rickettsia did not persist in adults of the two Encarsia species, while adults of both Eretmocerus species were virtually all Rickettsia-positive, even 12 days after they had emerged and fed on honey only (sample size = 10 wasps; 9 or 10 tested positive in each sample). In contrast, all Encarsia and Eretmocerus adults were Hamiltonella-negative (0/10 tested for each species).
The sequences obtained from the Rickettsia and Hamiltonella primers were 99% similar to the sequences of “Rickettsia endosymbiont of Bemisia tabaci” (DQ077707.1) and “secondary endosymbiont of Bemisia tabaci 16S ribosomal RNA gene” (AY429618.1) respectively. The Rickettsia 16S rRNA sequences obtained from B. tabaci and parasitoids in this study showed 100% similarity.
Localization of Rickettsia
Examination of the symbionts' localization by means of FISH shows a concentration of Rickettsia in the center of the Eretmocerus spp larval body in what seems to be the parasitoid's digestive tract, as well as scattered signals outside of the larval body in the remaining whitefly hemolymph (Fig. 2A). Later on, in the pupal stage, Rickettsia is aggregated in a kidney (or oval) shape within the wasp larva, and is more distal, toward the tip of the abdomen (Fig. 2B). Looking at an image without fluorescence shows an identical kidney-shaped concentration of small, dark spheres that are likely meconia (fecal material, typically retained within the wasp body until late in development) (Fig. 2C). In En. pergandiella, Rickettsia signals can be seen along the digestive tract of the crescent-shaped third instar larva as well as outside of the larva (Fig. 3A). In the pupal stage, however, Rickettsia is clearly present only in the meconia, deposited before pupation on both sides of the pre-pupal wasp (Fig. 3B). These FISH results are consistent with the results of the acquisition and sustainability experiment. In particular, they support the finding that adult En. pergandiella that developed on R+ whiteflies are not infected, and suggest that the detection of Rickettsia in pupal En. pergandiella by PCR is likely due to an extraction method that includes the whitefly cuticle and meconial pellets that surrounds the pupal wasp.
Left panel-Rickettsia probe fluorescent channel; right panel- overlay of fluorescent and brightfield channels. Arrows pointing to parasitoid gut. A- parasitoid larva (dark, ovoid sphere in the center of the host). Note Rickettsia in the parasitoid gut, as well in the whitefly's body remnants, surrounding the parasitoid. B- parasitoid pre-pupa. C- parasitoid pupae (note the autofluorescence of the anus and mouthpart); 1C, right image- brightfield channel only. D- parasitoid adult abdomen.
Left panel-Rickettsia probe fluorescent channel; right panel- overlay of fluorescent and brightfield channels. A- parasitoid larvae, arrow points to specific signal inside the larva body. B- parasitoid pupa, arrows pointing to the meconia deposited outside the parasitoid's body.
Electron micrographs of Er. eremicus reveal the presence of bacteria inside the ovaries, within follicular epithelial cells, but not within the oocytes (Fig. 4). Bacteria were also seen right outside the ovary, adjacent to the tunica propria, the ovarian envelope (Fig. 5). The germarium also shows bacteria among stem-, pre-follicle-, and nurse cell nuclei (Fig. 6). The determination that these bacteria are Rickettsia is supported by: 1) Denaturating gradient gel electrophoresis (DGGE) analysis of the bacteria present in Er. eremicus using general 16S rRNA primers that target most known bacteria. A single band, corresponding to Rickettsia was found in this analysis (data not shown). 2) Diagnostic PCR using specific primers designed for B. tabaci symbionts (Hamiltonella, Wolbachia, Cardinium, Arsenophonus and Rickettsia) showed bands only for Rickettsia in the Er. eremicus, as well as for the positive controls in all other cases (data not shown).
The gap between the follicular epithelial cell and the oocyte (the transition zone - TZ) is due to oocyte resorption. N-nucleus; EnC- endochorion; ExC- Exochorion; VE- Vitellin envelope.
FC- follicular epithelial cell; EnC- endochorion; ExC- Exochorion; VE- Vitellin envelope; Tr- Trachea.
Transmission from B. tabaci to parasitoids
Approximately 30% of the uninfected (R−) Eretmocerus adult wasps (from both species) that were exposed to R+ whiteflies as adults were subsequently infected with Rickettsia (Fig. 7A & 7B). The proportion of infected females was significantly higher than the proportion of infected males (Er. emiratus: 56% infected females vs. 6.7% infected males, χ232 = 8.8, P<0.01; Er. eremicus: 44% infected females vs. 11% infected males, χ243 = 5.8, P = 0.016), suggesting that host-feeding, in which females pierce hosts with their ovipositor and imbibe host hemolymph, is more likely a source of Rickettsia than feeding on honeydew (which both sexes do) or simple contact with contaminated insect surfaces. A much higher proportion of those wasps that developed inside R+ whiteflies were infected: 84% of Er. emiratus and 93% of Er. eremicus emerged as Rickettsia infected wasps (Fig. 7A & 7B). Thus, transmission of Rickettsia from infected whitefly hosts to Eretmocerus occurred at the greatest rate during parasitoid development, and to a much lower extent via host feeding by adults. All of the controls, i.e. R− wasps that were not exposed to any hosts and R− wasps that were exposed to R− hosts, were Rickettsia-free. Rickettsia infections that were acquired by host feeding seemed to be largely transient, as the proportion of infected females decreased sharply four days after removal from hosts (Er. emiratus: 15/20 infected immediately after exposure to hosts, vs. 2/10 infected four days later; Er. eremicus: 19/20 and 1/10 infected at the two time points, respectively). In contrast with the pattern seen for Rickettsia, Hamiltonella was not detected in any of the Eretmocerus wasps that fed or developed on Hamiltonella-infected whiteflies (0/26 tested).
‘P’ are R− wasps that were exposed to R+ whiteflies for 24 hrs (horizontal transmission via host feeding and/or honeydew), ‘F1’ are their resulting progeny that developed in R+ hosts (also horizontal transmission), and ‘F2’ are progeny of F1 that were exposed to R− hosts (vertical transmission). The numbers above the columns are the sample size, n, from which the proportion of infected wasps was calculated. See also Fig. 1 for this experiment's set-up.
Almost all (15 out of 16) of the adult females were infected with Rickettsia after exposure to R+ hosts, compared to only one infected male (χ232 = 25.5, P<0.0001) (Fig. 7C). Rickettsia acquired by host feeding and exposure to honeydew was also transient in En. pergandiella female adults: 17/20 were infected after exposure to R+ hosts, while 0/10 were infected four days later. None of the wasps that developed inside an R+ host were infected. As was found in Eretmocerus, Hamiltonella was also not detected in any of the En. pergandiella wasps exposed to infected whiteflies (0/23 tested).
Eretmocerus wasps that developed inside R− whitefly hosts emerged as uninfected wasps, even when their mothers were infected throughout their lifetime (0/20 Er. eremicus, 0/35 Er. emiratus infected, Fig. 7 A, B). There was no difference between the two experimental treatment groups, i.e., wasps with multiple generations of exposure to infected whiteflies prior to the experiment, and wasps with a single generation of exposure (parents). These experiments provide no evidence of vertical transmission of Rickettsia. Vertical transmission was not tested in En. pergandiella because Rickettsia infection did not persist in the adults of this species.
Interspecific horizontal transmission of facultative intracellular symbionts is believed to occur rarely, and little empirical evidence of such transfers exist [e.g. 20], , . That horizontal transmission between species must have occurred, however, is amply demonstrated in phylogenetic studies that show little concordance between host and symbiont phylogenies , . The results presented here demonstrate distinct transmission patterns of secondary symbionts between trophic levels and reveal differences in those patterns between two closely related parasitoid host genera. Further, we show that Rickettsia that is ingested during wasp larval development may penetrate the host hemocoel and infect the ovaries, but do not appear to invade the developing oocytes (Figs. 4–6), preventing vertical transmission in the wasp.
The variation we document in the transmission of Rickettsia from whiteflies to parasitoids highlights two possible views of horizontal transmission. From an evolutionary point of view (most often used in the symbiont literature), our results show no transmission of secondary symbionts from B. tabaci to parasitoids that result in a heritable infection. From a mechanistic point of view, however, we document the transmission of a microorganism from one individual to another, unrelated, individual within the same generation, a necessary precondition of a novel heritable infection in a population. Further, we show that symbionts acquired by feeding may be ultimately excreted (“contamination”), or invade the hemocoel and persist throughout the host lifetime, two distinct and sequential steps in the establishment of a long term association.
Rickettsia established a transtadial infection in Eretmocerus wasps, i.e. Rickettsia sustained in Eretmocerus from the larval stage through adulthood, but was not transmitted vertically. The FISH results indicate that Rickettsia was concentrated in the lower abdomen of the adult Eretmocerus wasps (Fig. 2C & 2D). The electron micrographs show that Rickettsia reached the ovaries of Eretmocerus but did not penetrate the germ line. Instead, it was found in the follicular epithelium surrounding the eggs and also in tissues abutting the ovaries (Figs. 4–6). The fact that Rickettsia is found within or in close proximity to the ovaries suggests that like other vertically transmitted bacteria, Rickettsia requires admission to the germ line for its spread and persistence in host insect populations. In their thorough study, Frydman et al  found that injected Wolbachia migrate and enter the Drosophila germline via the somatic stem cell niche in the germarium, from which follicular epithelial cells develop. Our results suggest, for Rickettsia at least, that invading the oocyte may require an adaptation distinct from the ability to find and invade the ovaries. Nonetheless, the inability of Rickettsia to invade the germ line of Eretmocerus may be a result of a defense mechanism of the latter.
The frequency of interspecific horizontal transmission in endosymbiosis of arthropods is clearly low and variable (excluding disease agents vectored by ticks etc). Possibly, the paucity of empirical studies conceals a number of unpublished negative results. Among published results, the frequency of Wolbachia horizontal transfer between Trichogramma species sharing a common host was 0–40% and the vertical transmission within the recipient species diminished within a few generations . Similarly, Spiroplasma was horizontally transmitted between two species of Drosophila by an ectoparasitic mite vector but the subsequent vertical transmission was very low . Variability of interspecific transmission success was also demonstrated in the study of Russell & Moran : pea aphids were injected with three different symbionts that were obtained from other aphid species. Two symbionts - Hamiltonella and Arsenophonus - were successfully established and maintained for multiple generations in their new host, whereas the third one – Regiella – was not. Grenier et al  reported successful horizontal transfer of Wolbachia from one species of Trichogramma to another via microinjection, followed by stable vertical transmission, but the efficiency of this process was low. To the best of our knowledge, the only study that describes symbiont horizontal transmission from a host to its parasitoid is that of Heath et al. , in which Wolbachia was weakly transmitted (3.2%) from an infected Drosophila host to a parasitoid, and subsequently diminished within four generations. Compared to these studies, the efficiency of Rickettsia transmission from the host, B. tabaci, to Eretmocerus wasps was very high and yet no vertical transmission was observed. Our results therefore support the notion that invasion of the germ line may be the greatest challenge for symbionts invading novel hosts. Among parasitoids, maternally transmitted Rickettsia was so far only found in a leaf miner parasitoid, where it causes parthenogenesis. However, it is not known whether this symbiont is present also in its hosts, which may be indicative of inter-trophic horizontal transmission .
Differences among hosts
Why does Rickettsia establish (even if for only one generation) in the Eretmocerus adults, whereas it appears to be completely excreted by the Encarsia? Encarsia embryos and larvae are in intimate, direct contact with the host's hemolymph throughout their development, whereas Eretmocerus become in contact with the host's hemolymph only in the third instar, due to the unique capsule in which the larval wasps reside . Hence, our finding that Eretmocerus acquire Rickettsia while Encarsia do not is, at first, counterintuitive. It is possible that these differences relate to the timing of the deposition of the meconium, fecal material. In En. pergandiella the mid-gut and the hind-gut are not continuous in early development, when the larva is in a fluid environment, but join only at the end of the third instar stage. Subsequently, the prepupal wasp deposits the meconium, with Rickettsia in it, and then pupates . Eretmocerus spp., in contrast, excrete the meconium only after the adult emerges, so meconia, with Rickettsia in them, are present in the body throughout metamorphosis. It may be that Rickettsia has the opportunity to invade new tissues during this phase, when tissues are breaking down and new ones are being built. This idea is supported by the observation that adult acquisition of the symbiont by consumption of honeydew or host hemolymph does not persist. Nevertheless, other routes of infection cannot be excluded: Rickettsia may get to the ovaries by crossing the larval mid-gut tissues, which in aphelinid larvae typically bear very few cells, no typical epithelium and no membranes (Dan Gerling, pers. comm.).
Differences between symbionts
Adult wasps of all species in our study acquired Rickettsia but not Hamiltonella from host feeding. One possible reason for that difference may be the localization of the two symbionts: Rickettsia is abundant and accessible in the host hemolymph consumed during the process of host-feeding while Hamiltonella is sequestered within the bacteriomes . Another explanation is required, however, for why Eretmocerus, during their development inside a host, acquire Rickettsia but not Hamiltonella, since the parasitoid larvae consume the entire host contents before pupation. Indeed, it seems that Rickettsia is generally more prone to horizontal transmission (e.g. to mammalian hosts in the case of the disease agents, or to plants in the case of insect-vectored plant pathogens) than many facultative intracellular symbiont lineages. To date, Rickettsia has been found in many host lineages , , whereas Hamiltonella has so far been revealed only in aphids, in whiteflies and in one psyllid species , . A possible mechanism for a greater propensity for horizontal transmission is greater symbiont mobility: while little is known about mobility in most symbiont lineages, some Rickettsia are able to move between cells and tissues using actin filaments , . We know nothing about the mobility of Hamiltonella, yet the fact that Hamiltonella reside in B. tabaci bacteriocytes where they are vertically transmitted along with the primary symbiont Portiera, suggests that Hamiltonella may have more limited mobility.
Naturally, an interesting question for further research would be to look for phenotypic effects of Rickettsia infection on the parasitoids. Preliminary results showed no differences between R+ and R− Er. emiratus with regards to fecundity, longevity and sex ratio, thus more fitness parameters need to be explored to address this question (Chiel et al., unpublished results).
To conclude, our study is one of few empirical demonstrations of the routes and barriers to horizontal transmission of facultative symbionts. These data are especially relevant to the often repeated idea that parasitoids or predators may be instrumental agents for moving symbionts from one host lineage to the next. In fact, this notion has some phylogenetic support [e.g. 12], , but in some cases, enemies have likely been wrongly diagnosed by PCR as being stably infected when the symbionts are simply present in the gut along with the prey or host material . Our data suggest that host-parasitoid transmission may, nonetheless, be one way in which symbionts acquire new hosts. Given that the symbiont is on the doorstep of vertical transmission, it is not hard to imagine that some lineage might, with time, acquire an adaptation that improves the precision of cell targeting in this new host lineage to get the symbiont over the threshold. Lastly, our study underscores how little we currently know about the processes of dispersal of symbionts to new host lineages, and the within-host movement and germ-line invasion processes necessary for them to stay once they get there.
We would like to thank David Bentley for assisting with analyzing the TEMs, and Ayelet Caspi-Fluger for assisting with the FISH. Technical assistance was provided by Hyo Kim, Seth Kyselka and Gaelen Burke. We would also like to thank four anonymous reviewers for their very constructive comments on the manuscript.
Conceived and designed the experiments: EC EZF TAH MH. Performed the experiments: EC YG TAH SEK MKA. Analyzed the data: EC EZF MI YG MH. Contributed reagents/materials/analysis tools: SEK. Wrote the paper: EC MH. Supervised the research: EZF MI MH. Reviewed the paper: EZF MI.
- 1. Baumann P (2005) Biology of bacteriocyte-associated endosymbionts of plant sap-sucking insects. Ann Rev Microbiol 59: 155–189.P. Baumann2005Biology of bacteriocyte-associated endosymbionts of plant sap-sucking insects.Ann Rev Microbiol59155189
- 2. Moran NA, McCutcheson JP, Nakabachi A (2008) Genomics and evolution of heritable bacterial symbionts. Annu Rev Genet 42: 165–190.NA MoranJP McCutchesonA. Nakabachi2008Genomics and evolution of heritable bacterial symbionts.Annu Rev Genet42165190
- 3. Hedges LM, Brownlie JC, O'Neill SL, Johnson KN (2008) Wolbachia and virus protection in insects. Science 322: 702.LM HedgesJC BrownlieSL O'NeillKN Johnson2008Wolbachia and virus protection in insects.Science322702
- 4. Montllor CB, Maxmen A, Purcell AH (2002) Facultative bacterial endosymbionts benefit pea aphids Acyrthosiphon pisum under heat stress. Ecol Entomol 27: 189–195.CB MontllorA. MaxmenAH Purcell2002Facultative bacterial endosymbionts benefit pea aphids Acyrthosiphon pisum under heat stress.Ecol Entomol27189195
- 5. Oliver KM, Moran NA, Hunter MS (2005) Variation in resistance to parasitism in aphids is due to symbionts not host genotype. Proc Nat Acad Sci USA 102: 12795–12800.KM OliverNA MoranMS Hunter2005Variation in resistance to parasitism in aphids is due to symbionts not host genotype.Proc Nat Acad Sci USA1021279512800
- 6. Oliver KM, Russell JA, Moran NA, Hunter MS (2003) Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proc Nat Acad Sci USA 100: 1803–1807.KM OliverJA RussellNA MoranMS Hunter2003Facultative bacterial symbionts in aphids confer resistance to parasitic wasps.Proc Nat Acad Sci USA10018031807
- 7. Scarborough CL, Ferrari J, Godfray HCJ (2005) Aphid protected from pathogen by endosymbiont. Science 310: 1781–1781.CL ScarboroughJ. FerrariHCJ Godfray2005Aphid protected from pathogen by endosymbiont.Science31017811781
- 8. Tsuchida T, Koga R, Fukatsu T (2004) Host plant specialization governed by facultative symbiont. Science 303: 1989–1989.T. TsuchidaR. KogaT. Fukatsu2004Host plant specialization governed by facultative symbiont.Science30319891989
- 9. Werren JH, Baldo L, Clark ME (2008) Wolbachia: master manipulators of invertebrate biology. Nature Rev Microbiol 6: 741–751.JH WerrenL. BaldoME Clark2008Wolbachia: master manipulators of invertebrate biology.Nature Rev Microbiol6741751
- 10. Russell JA, Latorre A, Sabater-Munoz B, Moya A, Moran NA (2003) Side-stepping secondary symbionts: widespread horizontal transfer across and beyond the Aphidoidea. Mol Ecol 12: 1061–1075.JA RussellA. LatorreB. Sabater-MunozA. MoyaNA Moran2003Side-stepping secondary symbionts: widespread horizontal transfer across and beyond the Aphidoidea.Mol Ecol1210611075
- 11. Viljakainen L, Reuter M, Pamilo P (2008) Wolbachia transmission dynamics in Formica wood ants. BMC Evol Biol 8: L. ViljakainenM. ReuterP. Pamilo2008Wolbachia transmission dynamics in Formica wood ants.BMC Evol Biol8
- 12. Vavre F, Fleury F, Lepetit D, Fouillet P, Bouletreau M (1999) Phylogenetic evidence for horizontal transmission of Wolbachia in host-parasitoid associations. Mol Biol Evol 16: 1711–1723.F. VavreF. FleuryD. LepetitP. FouilletM. Bouletreau1999Phylogenetic evidence for horizontal transmission of Wolbachia in host-parasitoid associations.Mol Biol Evol1617111723
- 13. Zchori-Fein E, Perlman SJ (2004) Distribution of the bacterial symbiont Cardinium in arthropods. Mol Ecol 13: 2009–2016.E. Zchori-FeinSJ Perlman2004Distribution of the bacterial symbiont Cardinium in arthropods.Mol Ecol1320092016
- 14. Werren JH, Zhang W, Guo LR (1995) Evolution and phylogeny of Wolbachia – reproductive parasites of arthropods. Proc R Soc Lond B Biol Sci 261: 55–63.JH WerrenW. ZhangLR Guo1995Evolution and phylogeny of Wolbachia – reproductive parasites of arthropods.Proc R Soc Lond B Biol Sci2615563
- 15. Werren JH, Skinner SW, Huger AM (1986) Male-killing bacteria in a parasitic wasp. Science 231: 990–992.JH WerrenSW SkinnerAM Huger1986Male-killing bacteria in a parasitic wasp.Science231990992
- 16. Huigens ME, Luck RF, Klaassen RHG, Maas M, Timmermans M, et al. (2000) Infectious parthenogenesis. Nature 405: 178–179.ME HuigensRF LuckRHG KlaassenM. MaasM. Timmermans2000Infectious parthenogenesis.Nature405178179
- 17. Varaldi J, Bouletreau M, Fleury F (2005) Cost induced by viral particles manipulating superparasitism behaviour in the parasitold Leptopilina boulardi. Parasitology 131: 161–168.J. VaraldiM. BouletreauF. Fleury2005Cost induced by viral particles manipulating superparasitism behaviour in the parasitold Leptopilina boulardi.Parasitology131161168
- 18. Moran NA, Dunbar HE (2006) Sexual acquisition of beneficial symbionts in aphids. Proc Nat Acad Sci USA 103: 12803–12806.NA MoranHE Dunbar2006Sexual acquisition of beneficial symbionts in aphids.Proc Nat Acad Sci USA1031280312806
- 19. Huigens ME, de Almeida RP, Boons PAH, Luck RF, Stouthamer R (2004) Natural interspecific and intraspecific horizontal transfer of parthenogenesis-inducing Wolbachia in Trichogramma wasps. Proc R Soc Lond B Biol Sci 271: 509–515.ME HuigensRP de AlmeidaPAH BoonsRF LuckR. Stouthamer2004Natural interspecific and intraspecific horizontal transfer of parthenogenesis-inducing Wolbachia in Trichogramma wasps.Proc R Soc Lond B Biol Sci271509515
- 20. Braig HR, Guzman H, Tesh RB, Oneill SL (1994) Replacement of the natural Wolbachia symbiont of Drosophila simulans with a mosquito counterpart. Nature 367: 453–455.HR BraigH. GuzmanRB TeshSL Oneill1994Replacement of the natural Wolbachia symbiont of Drosophila simulans with a mosquito counterpart.Nature367453455
- 21. Fujii Y, Kageyama D, Hoshizaki S, Ishikawa H, Sasaki T (2001) Transfection of Wolbachia in Lepidoptera: the feminizer of the adzuki bean borer Ostrinia scapulalis causes male killing in the Mediterranean flour moth Ephestia kuehniella. Proc R Soc Lond B Biol Sci 268: 855–859.Y. FujiiD. KageyamaS. HoshizakiH. IshikawaT. Sasaki2001Transfection of Wolbachia in Lepidoptera: the feminizer of the adzuki bean borer Ostrinia scapulalis causes male killing in the Mediterranean flour moth Ephestia kuehniella.Proc R Soc Lond B Biol Sci268855859
- 22. Grenier S, Pintureau B, Heddi A, Lassabliere F, Jager C, et al. (1998) Successful horizontal transfer of Wolbachia symbionts between Trichogramma wasps. Proc R Soc Lond B Biol Sci 265: 1441–1445.S. GrenierB. PintureauA. HeddiF. LassabliereC. Jager1998Successful horizontal transfer of Wolbachia symbionts between Trichogramma wasps.Proc R Soc Lond B Biol Sci26514411445
- 23. Xi ZY, Khoo CCH, Dobson SL (2006) Interspecific transfer of Wolbachia into the mosquito disease vector Aedes albopictus. Proc R Soc Lond B Biol Sci 273: 1317–1322.ZY XiCCH KhooSL Dobson2006Interspecific transfer of Wolbachia into the mosquito disease vector Aedes albopictus.Proc R Soc Lond B Biol Sci27313171322
- 24. Kageyama D, Narita S, Noda H (2008) Transfection of feminizing Wolbachia endosymbionts of the butterfly, Eurema hecabe, into the cell culture and various immature stages of the silkmoth, Bombyx mori. Microb Ecol 56: 733–741.D. KageyamaS. NaritaH. Noda2008Transfection of feminizing Wolbachia endosymbionts of the butterfly, Eurema hecabe, into the cell culture and various immature stages of the silkmoth, Bombyx mori.Microb Ecol56733741
- 25. Russell JA, Moran NA (2005) Horizontal transfer of bacterial symbionts: Heritability and fitness effects in a novel aphid host. Appl Environ Microbiol 71: 7987–7994.JA RussellNA Moran2005Horizontal transfer of bacterial symbionts: Heritability and fitness effects in a novel aphid host.Appl Environ Microbiol7179877994
- 26. Frydman HM, Li JM, Robson DN, Wieschaus E (2006) Somatic stem cell niche tropism in Wolbachia. Nature 441: 509–512.HM FrydmanJM LiDN RobsonE. Wieschaus2006Somatic stem cell niche tropism in Wolbachia.Nature441509512
- 27. Oliveira MRV, Henneberry TJ, Anderson P (2001) History, current status, and collaborative research projects for Bemisia tabaci. Crop Prot 20: 709–723.MRV OliveiraTJ HenneberryP. Anderson2001History, current status, and collaborative research projects for Bemisia tabaci.Crop Prot20709723
- 28. Thao ML, Baumann P (2004) Evolutionary relationships of primary prokaryotic endosymbionts of whiteflies and their hosts. App Environ Microbiol 70: 3401–3406.ML ThaoP. Baumann2004Evolutionary relationships of primary prokaryotic endosymbionts of whiteflies and their hosts.App Environ Microbiol7034013406
- 29. Chiel E, Gottlieb Y, Zchori-Fein E, Mozes-Daube N, Katzir N, et al. (2007) Biotype-dependent secondary symbiont communities in sympatric populations of Bemisia tabaci. Bull Entomol Res 97: 407–413.E. ChielY. GottliebE. Zchori-FeinN. Mozes-DaubeN. Katzir2007Biotype-dependent secondary symbiont communities in sympatric populations of Bemisia tabaci.Bull Entomol Res97407413
- 30. Gottlieb Y, Ghanim M, Gueguen G, Kontsedalov S, Vavre F, et al. (2008) Inherited intracellular ecosystem: symbiotic bacteria share bacteriocytes in whiteflies. FASEB Journal 22: 2591–2599.Y. GottliebM. GhanimG. GueguenS. KontsedalovF. Vavre2008Inherited intracellular ecosystem: symbiotic bacteria share bacteriocytes in whiteflies.FASEB Journal2225912599
- 31. Perlman SJ, Hunter MS, Zchori-Fein E (2006) The emerging diversity of Rickettsia. Proc R Soc Lond B Biol Sci 273: 2097–2106.SJ PerlmanMS HunterE. Zchori-Fein2006The emerging diversity of Rickettsia.Proc R Soc Lond B Biol Sci27320972106
- 32. Gottlieb Y, Ghanim M, Chiel E, Gerling D, Portnoy V, et al. (2006) Identification and localization of a Rickettsia sp in Bemisia tabaci (Homoptera : Aleyrodidae). App Environ Microbiol 72: 3646–3652.Y. GottliebM. GhanimE. ChielD. GerlingV. Portnoy2006Identification and localization of a Rickettsia sp in Bemisia tabaci (Homoptera : Aleyrodidae).App Environ Microbiol7236463652
- 33. Chiel E, Inbar M, Mozes-Daube N, White JA, Hunter MS, et al. (2009) Assessments of fitness effects by the facultative symbiont, Rickettsia, in the sweetpotato whitefly (Hemiptera: Aleyrodidae). Ann Entomol Soc Am (in press). E. ChielM. InbarN. Mozes-DaubeJA WhiteMS Hunter2009Assessments of fitness effects by the facultative symbiont, Rickettsia, in the sweetpotato whitefly (Hemiptera: Aleyrodidae).Ann Entomol Soc Am (in press)
- 34. Kontsedalov S, Zchori-Fein E, Chiel E, Gottlieb Y, Inbar M, et al. (2008) The presence of Rickettsia is associated with increased susceptibility of Bemisia tabaci (Homoptera: Aleyrodidae) to insecticides. Pest Manag Sci 64: 789–792.S. KontsedalovE. Zchori-FeinE. ChielY. GottliebM. Inbar2008The presence of Rickettsia is associated with increased susceptibility of Bemisia tabaci (Homoptera: Aleyrodidae) to insecticides.Pest Manag Sci64789792
- 35. Moran NA, Degnan PH, Santos SR, Dunbar HE, Ochman H (2005) The players in a mutualistic symbiosis: Insects, bacteria, viruses, and virulence genes. Proc Nat Acad Sci USA 102: 16919–16926.NA MoranPH DegnanSR SantosHE DunbarH. Ochman2005The players in a mutualistic symbiosis: Insects, bacteria, viruses, and virulence genes.Proc Nat Acad Sci USA1021691916926
- 36. Gerling D, Alomar O, Arno J (2001) Biological control of Bemisia tabaci using predators and parasitoids. Crop Prot 20: 779–799.D. GerlingO. AlomarJ. Arno2001Biological control of Bemisia tabaci using predators and parasitoids.Crop Prot20779799
- 37. Zolnerowich G (2008) Status of Eretmocerus (Hymenoptera : Aphelinidae) systematics. J Insect Sci 8: 54–54.G. Zolnerowich2008Status of Eretmocerus (Hymenoptera : Aphelinidae) systematics.J Insect Sci85454
- 38. Heraty J, Woolley J, Polaszek A (2007) J. HeratyJ. WoolleyA. Polaszek2007Catalog of the Encarsia of the World, Hymenopteran systematics, UC Riverside website, http://hymenoptera.ucr.edu/. Catalog of the Encarsia of the World, Hymenopteran systematics, UC Riverside website, http://hymenoptera.ucr.edu/.
- 39. Gerling D, Orion T, Delarea Y (1990) Eretmocerus penetration and immature development – a novel approach to overcome host immunity. Arch Insect Biochem Physiol 13: 247–253.D. GerlingT. OrionY. Delarea1990Eretmocerus penetration and immature development – a novel approach to overcome host immunity.Arch Insect Biochem Physiol13247253
- 40. Gerling D (1966) Studies with whitefly parasites of southern California. I. Encarsia pergandiella Howard (Hymenoptera-Aphelinidae). Can Entomol 98: 707–724.D. Gerling1966Studies with whitefly parasites of southern California. I. Encarsia pergandiella Howard (Hymenoptera-Aphelinidae).Can Entomol98707724
- 41. Hunter MS, Woolley JB (2001) Evolution and behavioral ecology of heteronomous aphelinid parasitoids. Ann Rev Entomol 46: 251–290.MS HunterJB Woolley2001Evolution and behavioral ecology of heteronomous aphelinid parasitoids.Ann Rev Entomol46251290
- 42. Asplen MK, Byrne DN (2006) Quantification and ultrastructure of oosorption in Eretmocerus eremicus (Hymenoptera : Aphelinidae). J Morphol 267: 1066–1074.MK AsplenDN Byrne2006Quantification and ultrastructure of oosorption in Eretmocerus eremicus (Hymenoptera : Aphelinidae).J Morphol26710661074
- 43. Jaenike J, Polak M, Fiskin A, Helou M, Minhas M (2007) Interspecific transmission of endosymbiotic Spiroplasma by mites. Biol Lett 3: 23–25.J. JaenikeM. PolakA. FiskinM. HelouM. Minhas2007Interspecific transmission of endosymbiotic Spiroplasma by mites.Biol Lett32325
- 44. Heath BD, Butcher RDJ, Whitfield WGF, Hubbard SF (1999) Horizontal transfer of Wolbachia between phylogenetically distant insect species by a naturally occurring mechanism. Curr Biol 9: 313–316.BD HeathRDJ ButcherWGF WhitfieldSF Hubbard1999Horizontal transfer of Wolbachia between phylogenetically distant insect species by a naturally occurring mechanism.Curr Biol9313316
- 45. Hagimori T, Abe Y, Date S, Miura K (2006) The first finding of a Rickettsia bacterium associated with parthenogenesis induction among insects. Curr Microbiol 52: 97–101.T. HagimoriY. AbeS. DateK. Miura2006The first finding of a Rickettsia bacterium associated with parthenogenesis induction among insects.Curr Microbiol5297101
- 46. Walker DH, Ismail N (2008) Emerging and re-emerging Rickettsioses: endothelial cell infection and early disease events. Nature Rev Microbiol 6: 375–386.DH WalkerN. Ismail2008Emerging and re-emerging Rickettsioses: endothelial cell infection and early disease events.Nature Rev Microbiol6375386
- 47. Ogata H, La Scola B, Audic S, Renesto P, Blanc G, et al. (2006) Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens. Plos Genetics 2: 733–744.H. OgataB. La ScolaS. AudicP. RenestoG. Blanc2006Genome sequence of Rickettsia bellii illuminates the role of amoebae in gene exchanges between intracellular pathogens.Plos Genetics2733744
- 48. van Meer MMM, Witteveldt J, Stouthamer R (1999) Phylogeny of the arthropod endosymbiont Wolbachia based on the wsp gene. Insect Mol Biol 8: 399–408.MMM van MeerJ. WitteveldtR. Stouthamer1999Phylogeny of the arthropod endosymbiont Wolbachia based on the wsp gene.Insect Mol Biol8399408
- 49. Enigl M, Zchori-Fein E, Schausberger P (2005) Negative evidence of Wolbachia in the predaceous mite Phytoseiulus persimilis. Exp Appl Acarol 36: 249–262.M. EniglE. Zchori-FeinP. Schausberger2005Negative evidence of Wolbachia in the predaceous mite Phytoseiulus persimilis.Exp Appl Acarol36249262