Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

The Warburg Effect Suppresses Oxidative Stress Induced Apoptosis in a Yeast Model for Cancer

The Warburg Effect Suppresses Oxidative Stress Induced Apoptosis in a Yeast Model for Cancer

  • Christoph Ruckenstuhl, 
  • Sabrina Büttner, 
  • Didac Carmona-Gutierrez, 
  • Tobias Eisenberg, 
  • Guido Kroemer, 
  • Stephan J. Sigrist, 
  • Kai-Uwe Fröhlich, 
  • Frank Madeo
PLOS
x

Abstract

Background

Otto Warburg observed that cancer cells are often characterized by intense glycolysis in the presence of oxygen and a concomitant decrease in mitochondrial respiration. Research has mainly focused on a possible connection between increased glycolysis and tumor development whereas decreased respiration has largely been left unattended. Therefore, a causal relation between decreased respiration and tumorigenesis has not been demonstrated.

Methodology/Principal Findings

For this purpose, colonies of Saccharomyces cerevisiae, which is suitable for manipulation of mitochondrial respiration and shows mitochondria-mediated cell death, were used as a model. Repression of respiration as well as ROS-scavenging via glutathione inhibited apoptosis and conferred a survival advantage during seeding and early development of this fast proliferating solid cell population. In contrast, enhancement of respiration triggered cell death.

Conclusion/Significance

Thus, the Warburg effect might directly contribute to the initiation of cancer formation - not only by enhanced glycolysis - but also via decreased respiration in the presence of oxygen, which suppresses apoptosis.

Introduction

In the 1920's Otto Warburg described the most common biochemical phenotype of tumor cells: even in the presence of a normal oxygen level, glycolysis is highly elevated and accompanied by high lactate production as well as drastically reduced mitochondrial respiration [1][3]. Though this phenotype is the basis for tumor diagnostics monitoring glucose consumption [4], challenging questions remain elusive: For example how tumor cells adopt this phenotype by different mechanisms [5], [6], whether both phenomena (high glycolysis and decreased respiration) are causally linked [7], [8], and finally their specific contribution to tumorigenesis [7], [9][11].

Rapidly proliferating yeasts show similar fermentation values as tumor cells [12], thus allowing to study the relationship between energy metabolism and maximal proliferation. Notably, programmed cell death, which protects against tumorigenesis, is intimately linked to mitochondrial processes in mammalian cells. Yeast cells can also undergo programmed cell death [13], [14] in a mitochondria-dependent manner [15], [16], including cytochrome c and AIF release, channel opening upon human Bax expression [17][19], depolarisation of mitochondrial membrane potential [20], and mitochondrial fragmentation [21].

The special ability of Crabtree-positive yeasts like Saccharomyces cerevisiae to switch on and off respiration in response to changes in the carbon source and the possibility of producing viable strains lacking mitochondrial DNA (rho0), allow the stringent genetic evaluation of the role of mitochondria during cell death [15], [22]. Here, we assess the possible link between suppressed cell death and inhibited oxidative phosphorylation, in colonies grown from a single cell, resembling tumor growth.

Results and Discussion

In a maximal proliferating solid cell population, respiration enhances ROS production and apoptosis

S. cervevisiae cells inherit a unique glucose repression system that upon growth on glucose drastically suppresses respiration independently of oxygen availability [23]. Modelling tumor growth, we tested if repressed respiration influences cell-survival during growth of cell populations starting from a single cell on glucose media. Three different strains incapable of respiration together with isogenic wild-type S. cerevisiae were used in a colony assay where isolated colonies were grown from single cells. Cells removed from colonies during early development of a rho0 strain colony exhibit significantly increased survival compared to the wild-type strain (Fig. 1A). Consistently, the generation of reactive oxygen species (ROS), a process tightly connected to and often causative for programmed cell death, is diminished (Fig. 1B).

thumbnail
Figure 1. Abrogation of respiration suppresses apoptosis and ROS production in a maximal proliferating solid cell population.

(A) Isolated colonies were grown from single cells, manipulated onto agar plates. Clonogenic assay of cells removed from colonies of the wild-type strain, rho0 (no mitochondrial DNA) and two single gene-deletion strains (mgm1 and oxa1), grown on SCGlu. After 3 days 500 cells of whole colonies, after 5 days 500 cells of the central colony-region were analysed (mean±SEM, n = 2). Statistical significance of p<0.006 compares colony forming units (cfu) of mutant strains to wild-type strain at respective time-points. (B) Cells from the whole colony (3 days) as well as from the central region (5 days) were stained for reactive oxygen species (ROS) with dihydroethidium and analysed by FACSAria flow cytometry (mean±SEM, n = 2; **p<0.01, ***p<0.001 compared to deletion strains at respective time-points). (C) Approximately 50 (2 days) and 10 (3 days) colonies grown on SCGlu plates were washed off and clonogenic assays were performed with the collected cells (mean±SEM, n = 5; **p<0.01, ***p<0.001). (D) Approximately 50 colonies grown on SCGlu plates for 2 days were stained for phosphatidylserine exposition (AnnV) and DNA breakage (TUNEL) and analyzed by FACSAria flow cytometry (mean±SEM, n = 3; **p<0.01, ***p<0.001). (E) Fluorescence microscopy from central and outer region of 5 days old wild-type colony cells, harbouring plasmid dsRED-MLS.

https://doi.org/10.1371/journal.pone.0004592.g001

To exclude rho0 strain specific effects not accounting for respiration deficiencies, two other respiration impaired strains were investigated. Single gene deletion strains of both, MGM1 (homolog to human OPA1) - a GTPase located in the mitochondrial intermembrane space [24] - and OXA1 (an insertase of the inner mitochondrial membrane – highly conserved from prokaryotes to mammals [25], [26]) behaved like the (wild-type) rho0 strain. After 3 and 5 days, mgm1 as well as oxa1 deleted cells retrieved from the whole colony (3 days) or the central region (5 days), and thus mainly older cells, showed significantly increased survival (Fig. 1A) as well as inhibition of ROS generation compared to samples of the wild-type strain (Fig. 1B). Of note, unlike other respiration deficient mutant strains (including rho0 and Δmgm1) the Δoxa1 strain showed same growth rates as the wild-type strain in liquid cultures and during colony growth, respectively (Fig. 2A and B).

thumbnail
Figure 2. An oxa1 deletion does not influence the strains growth rate.

(A) Growth curves of an oxa1 deletion strain and the corresponding wild-type strain. Experiment was performed in triplicate. The y-axis is scaled logarithmically. (B) Size of isolated colonies of the indicated strains grown for 3 and 5 days on SCGlu plates, respectively. Diameters were evaluated by processing the photos with Metamorph Imaging (mean±SEM, n = 2).

https://doi.org/10.1371/journal.pone.0004592.g002

In a complementary approach, seeded colonies (10 and 50 per plate) of wild-type and Δoxa1 strains, respectively, were washed from SCGlu plates after 2 and 3 days and clonogenic survival was evaluated. Again, the strain incapable of respiration showed better survival than the respiring wild-type strain (Fig. 1C). Additionally, the survival advantage of the Δoxa1 strain occurred already after 2 days, which is in good accordance with previous data that wild-type colonies grown on the usual glucose media where shown to leave the logarithmic growth phase after about 36 hours [27].

To evaluate the induced type of cell death we screened for apoptotic markers using AnnexinV and TUNEL assays. Apoptosis was significantly decreased in a respiration deficient oxa1 deletion mutant compared to the wild-type control (Fig. 1D). It should be noted that cell wall digestion of wild-type cells was less efficient. Therefore lower amounts of positively stained cells were obtained as compared to the observed cell survival in clonogenic assays after 2 days. This means that the observed suppression of apoptotic markers in the oxa1 deleted strain is probably even higher than shown in 1D. In this line when treated with 30% more enzyme mix to enhance cell wall digestion, approximately 37% of wild-type cells displayed a TUNEL positive staining (data not shown). Mitochondrial fragmentation is a prerequisite for apoptosis in yeast. Therefore, mitochondrial morphology of cells from wild-type colonies (and not for rho0 cells, because they lack respiration) was monitored using dsRED carrying a mitochondrial localization sequence. After 5 days, cells from the central region (thus older cells) showed a drastically increased fragmentation of mitochondria and more punctured structures whereas freshly nourished younger cells from the outer rim displayed a normal tubular structure of mitochondria (Fig 1E).

We conclude that abrogation of respiration suppresses apoptosis and ROS production in a strongly proliferating cell population on solid media.

Forced enhancement of respiration triggers cell death during seeding of a cell population

Novel cancer therapies are based on the assumption that reconstitution or forced enhancement of respiration within solid tumors may lead to growth inhibition and cell death [10], [28], [29]. To test whether genetic enforcement of respiration influences cell death during seeding of a cell population (which is the chronological step before a colony establishes), media-shift experiments using glucose (SCGlu), galactose (SCGal), and glycerol (SCGly) media as alternating sole carbon sources were performed. Thereby, respiration is either suppressed (SCGlu), cooperatively active with fermentation (SCGal), or it represents the exclusive energy source (SCGly). When stationary (non proliferating) liquid populations of yeast wild-type cells grown on SCGlu were shifted to solid SCGal or SCGly, only 65% or 44%, respectively, of seeded cells were able to form a colony (Fig. 3A). Using highly proliferative cultures of wild-type BY4741 strain (from the exponential growth phase) this effect was even more pronounced with nearly no survival on both respiratory media (Fig. 3A). Comparable results were obtained with highly proliferative cultures of other wild-type strains such as AH22, TB50, and DBY746 (data not shown) under our conditions. Even a transfer in the same high respiratory media (from liquid SCGly to solid SCGly) led to a diminished colony formation of ∼70% (compared to a shift from liquid SCGly to solid SCGlu), underlining that specifically the initiation of colony growth is negatively affected by enhanced respiration (Fig. 3B).

thumbnail
Figure 3. Forced enhancement of respiration triggers cell death, suppresses growth, and elevates ROS production.

(A) Clonogenic assay of cultures pre-grown in liquid SCGlu. 500 cells were plated on SCGlu, SCGal, and SCGly agar plates, respectively (mean±SEM, n = 3; ***p<0.001). (B) Clonogenic assay of cultures pre-grown in liquid SCGly. 500 cells were plated on SCGly and SCGlu media plates, respectively (mean±SEM, n = 2; **p<0.01). (C) Cells from the whole colony (3 days) as well as from the central region (5 days) were stained for reactive oxygen species (ROS) with dihydroethidium (DHE). Values of relative fluorescent units (RFU) were normalized to wild-type strain values (mean±SEM, n = 2; **p<0.01, ***p<0.001). (D) Size of isolated wild-type colonies grown on SCGlu and SCGal media plates were monitored by taking photos at indicated time-points. Diameters were evaluated by processing the photos with Metamorph Imaging (white bar represents 1 cm) (mean±SEM, n = 3; *p<0.05, **p<0.01).

https://doi.org/10.1371/journal.pone.0004592.g003

Forced enhancement of respiration suppresses growth and increases ROS production of colonies

The observed effects of suppressed colony seeding when respiration is high were also reflected in the further growth of the cell population: After two days of growth on solid SCGal, the colonies displayed only half the size of those grown on solid SCGlu, though further size increase (after the initial delay) is comparable (Fig. 3D). The initial delay could be associated with an immediate ROS burst, as further underlined by drastically elevated ROS levels in colonies grown on SCGal or SCGly (Fig. 3C, also see below and Fig. 4C).

thumbnail
Figure 4. Administration of GSH diminishes ROS levels and enhances survival within wild-type colonies, as well as during seeding on highly respiratory media.

(A) Approximately 100 colonies were grown for 36 hours on SCGlu plates with or without 5 mM GSH, washed off and clonogenic assays were performed with the collected cells (mean±SEM, n = 3; **p<0.01). Additionally equal amounts of cells were stained for reactive oxygen species (ROS) with dihydroethidium (DHE) and quantified using a fluorescence microplate reader (Genios-Pro). Values of relative fluorescent units (RFU) are displayed (mean±SEM, n = 3; **p<0.01) (B). (C) Clonogenic assay of cultures pre-grown in liquid SCGlu. 500 cells were plated on SCGlu, SCGal, or SCGly agar plates, respectively, with or without 1 mM GSH (mean±SEM, n = 2; ***p<0.001).

https://doi.org/10.1371/journal.pone.0004592.g004

Thus, establishing a strongly proliferating solid cell population requires respiration to be repressed.

Respiration mediated cell death in colonies and newly seeded cells is efficiently suppressed via ROS scavenging

To further prove that respiration and accompanying ROS production is a major cause of apoptosis during colony development we used reduced glutathione (GSH) as a scavenging reagent: Colonies of wild-type and oxa1 deletion strains were grown on SCGlu agar plates with or without GSH and cell survival was determined after 36 hours in a clonogenic assay. Whereas the oxa1 deletion strain showed no altered survival upon addition of GSH, survival of wild-type cells was drastically enhanced (Fig 4A). This was accompanied by significantly diminished ROS levels (Fig 4B). Interestingly, extending the fermentative growth phase by doubling glucose concentration in the media plates led to slightly enhanced cell survival (data not shown).

Additionally, the effect of glutathione as ROS scavenger was tested in a shift assay, where highly proliferative wild-type cells pregrown in liquid SCGlu media were seeded onto SCGal and SCGly plates with or without GSH. Strikingly, survival was enhanced to 50% and 55%, respectively, compared to <1% survival on plates without GSH (Fig. 4C). A similar shift of stationary cells yielded an increased survival from 50% and 60% to 75% and 77%, respectively, on GSH containing plates (data not shown). We conclude that scavenging ROS via glutathione enhances cell survival during colony development as well as during seeding on highly respiratory media. Intriguingly, it has been reported recently that in cancer cells and in neurons (that also display the Warburg effect), cytochrome c is reduced and held inactive by GSH [30].

So far, research on the Warburg effect has mainly focused on the high glycolytic flux while the accompanying repression of mitochondrial respiration was often left unattended [8], [9]. Our data demonstrate that respiration triggers apoptosis during seeding and development of a cell population, providing direct experimental evidence in support of the Warburg hypothesis during initiation of tumorigenesis. The strongly decreased respiratory capacity may be crucial for tumor malignancy [12] and for resistance against inevitable fluctuating oxygenation in tumors [31], [32]. Interestingly, it has been demonstrated that yeast rho0 strains exhibit increased resistance towards certain external apoptotic stimuli such as acetic acid, which triggers death via the mitochondrial pathway [15], [17]. A similar resistance against cell death may also play a role for tumor cells in an acidic surrounding. We speculate that reprogramming of cancer cells follows an ancient mechanism present in S. cerevisiae wild-type colonies: high glycolysis in the presence of oxygen.

Materials and Methods

Strains and media

Experiments were carried out in BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) and respective null mutants of mgm1 and oxa1, respectively, obtained from Euroscarf. Strain BY4741 rho0 was constructed by several rounds of ethidiumbromide treatment as previously described [33]. To perform fluorescence microscopy of mitochondrial structures the plasmid dsRED-MLS (dsRED carrying a mitochondrial localization sequence) [34] was transformed in strain BY4741. For survival platings YEPD (1% yeast extract, 2% peptone, and 2% glucose) media was supplemented with 2% agar. For all assays, strains were grown in SC medium containing 0.17% yeast nitrogen base (Difco), 0.5% (NH4)2SO4, and 30 mg/l of all amino acids (except 80 mg/l histidine, 200 mg/l leucine (without leucine when working with plasmid dsRED-MLS)), 30 mg/l adenine, and 320 mg/l uracil with 2% glucose (SCGlu), 2% galactose (SCGal), and 3% glycerol (SCGly), respectively, supplemented with 2% agar, where needed. Reduced glutathione (GSH; Sigma-Aldrich) was added at concentrations of 1 or 5 mM, where needed.

Isolated monocolony assay

Six centimeter petridishes, supplied with 13 ml of solid SC media (with or without leucine) were used. Single cells of 16 to 20 hours overnight cultures were placed onto solid SC media plates using a micromanipulator (Series 200, Singer Instruments). To minimize dehydration of the solid plates, incubation was performed at 28°C in a closed, humidified breeding chamber (KBF115, Binder). For diameter determination, photos were taken at the indicated time-points (Microbiology-Colony-Counter, Lemnatec) and processed using Metamorph imaging.

Survival plating, media-shift experiments, and growth curves

To determine survival of cells within the colony, either whole isolated colonies (in the case of 3 days) and samples from the central regions of the isolated colonies (in the case of 5 days) were removed at indicated time-points, or SCGlu plates with approximately 100 colonies (after 36 hours), 50 colonies (after 2 days) or 10 colonies (after 3 days) were washed off. Cell counts were determined by a CASY cell counting device (Schärfe Systems) and 500 cells were plated on YEPD agar plates. Colony forming units (cfu) were determined after 2 or 3 days (in the case of respiration deficient strains rho0 and Δmgm1) with a Microbiology-Colony-Counter (Lemnatec) and processed using SAWmicrobio version 3.1.

For media-shift experiments, cultures were grown in SCGlu and SCGly liquid media to logarithmic (6 h) and stationary (24 h) phase, respectively, and 500 cells were plated on respective solid media plates as well as on SCGlu plates in any case.

For assessment of growth rates in liquid media, overnight cultures were grown in SCGlu. Cultures were inoculated to 5×105 cells in SCGlu and cell growth was monitored over 12 hours using a CASY cell counter device.

Staining for apoptotic markers and fluorescence microscopy

Staining of reactive oxygen species (ROS) with dihydroethidium (DHE), Annexin V-staining, and TUNEL-staining were performed as described previously [34]. In each sample 30.000 cells were evaluated using flow cytometry (FACS-Aria, BD) and processed using FACSDiva software. Alternatively equal amounts of DHE stained cells were measured in a microplate fluorescence reader (Genios-Pro, Tecan).

Mitochondrial morphology was inspected by fluorescence microscopy using a dsRED filter on a Zeiss Axioskop microscope. Cells of the central as well as the outer region of isolated monocolonies of wild-type cells harbouring plasmid dsRED-MLS were monitored after 5 days.

Statistical analysis

All statistical analyses were performed using Students T-Test (one-tailed, unpaired), with *p<0.05, **p<0.01 and ***p<0.001, respectively.

Acknowledgments

We thank Ulrike Potocnik for technical assistance.

Author Contributions

Conceived and designed the experiments: CR KUF FM. Performed the experiments: CR SB DCG TE. Analyzed the data: CR GK SJS FM. Contributed reagents/materials/analysis tools: SB. Wrote the paper: CR SB DCG GK SJS KUF FM.

References

  1. 1. Warburg O (1956) On respiratory impairment in cancer cells. Science 124: 269–270.O. Warburg1956On respiratory impairment in cancer cells.Science124269270
  2. 2. Kondoh H (2008) Cellular life span and the Warburg effect. Exp Cell Res 314: 1923–1928.H. Kondoh2008Cellular life span and the Warburg effect.Exp Cell Res31419231928
  3. 3. Pedersen PL (1978) Tumor mitochondria and the bioenergetics of cancer cells. Prog Exp Tumor Res 22: 190–274.PL Pedersen1978Tumor mitochondria and the bioenergetics of cancer cells.Prog Exp Tumor Res22190274
  4. 4. Gambhir SS (2002) Molecular imaging of cancer with positron emission tomography. Nat Rev Cancer 2: 683–693.SS Gambhir2002Molecular imaging of cancer with positron emission tomography.Nat Rev Cancer2683693
  5. 5. Gordan JD, Thompson CB, Simon MC (2007) HIF and c-Myc: sibling rivals for control of cancer cell metabolism and proliferation. Cancer Cell 12: 108–113.JD GordanCB ThompsonMC Simon2007HIF and c-Myc: sibling rivals for control of cancer cell metabolism and proliferation.Cancer Cell12108113
  6. 6. Pedersen PL (2007) Warburg, me and Hexokinase 2: Multiple discoveries of key molecular events underlying one of cancers' most common phenotypes, the “Warburg Effect”, i.e., elevated glycolysis in the presence of oxygen. J Bioenerg Biomembr 39: 211–222.PL Pedersen2007Warburg, me and Hexokinase 2: Multiple discoveries of key molecular events underlying one of cancers' most common phenotypes, the “Warburg Effect”, i.e., elevated glycolysis in the presence of oxygen.J Bioenerg Biomembr39211222
  7. 7. Denko NC (2008) Hypoxia, HIF1 and glucose metabolism in the solid tumour. Nat Rev Cancer. NC Denko2008Hypoxia, HIF1 and glucose metabolism in the solid tumour.Nat Rev Cancer
  8. 8. Garber K (2006) Energy deregulation: licensing tumors to grow. Science 312: 1158–1159.K. Garber2006Energy deregulation: licensing tumors to grow.Science31211581159
  9. 9. DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB (2008) The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab 7: 11–20.RJ DeBerardinisJJ LumG. HatzivassiliouCB Thompson2008The biology of cancer: metabolic reprogramming fuels cell growth and proliferation.Cell Metab71120
  10. 10. Gogvadze V, Orrenius S, Zhivotovsky B (2008) Mitochondria in cancer cells: what is so special about them? Trends Cell Biol 18: 165–173.V. GogvadzeS. OrreniusB. Zhivotovsky2008Mitochondria in cancer cells: what is so special about them?Trends Cell Biol18165173
  11. 11. Kaelin WG Jr (2008) The von Hippel-Lindau tumour suppressor protein: O2 sensing and cancer. Nat Rev Cancer 8: 865–873.WG Kaelin Jr2008The von Hippel-Lindau tumour suppressor protein: O2 sensing and cancer.Nat Rev Cancer8865873
  12. 12. Warburg O (1956) On the origin of cancer cells. Science 123: 309–314.O. Warburg1956On the origin of cancer cells.Science123309314
  13. 13. Madeo F, Frohlich E, Frohlich KU (1997) A yeast mutant showing diagnostic markers of early and late apoptosis. J Cell Biol 139: 729–734.F. MadeoE. FrohlichKU Frohlich1997A yeast mutant showing diagnostic markers of early and late apoptosis.J Cell Biol139729734
  14. 14. Madeo F, Frohlich E, Ligr M, Grey M, Sigrist SJ, et al. (1999) Oxygen stress: a regulator of apoptosis in yeast. J Cell Biol 145: 757–767.F. MadeoE. FrohlichM. LigrM. GreySJ Sigrist1999Oxygen stress: a regulator of apoptosis in yeast.J Cell Biol145757767
  15. 15. Eisenberg T, Buttner S, Kroemer G, Madeo F (2007) The mitochondrial pathway in yeast apoptosis. Apoptosis 12: 1011–1023.T. EisenbergS. ButtnerG. KroemerF. Madeo2007The mitochondrial pathway in yeast apoptosis.Apoptosis1210111023
  16. 16. Pereira C, Silva RD, Saraiva L, Johansson B, Sousa MJ, et al. (2008) Mitochondria-dependent apoptosis in yeast. Biochim Biophys Acta 1783: 1286–1302.C. PereiraRD SilvaL. SaraivaB. JohanssonMJ Sousa2008Mitochondria-dependent apoptosis in yeast.Biochim Biophys Acta178312861302
  17. 17. Ludovico P, Rodrigues F, Almeida A, Silva MT, Barrientos A, et al. (2002) Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae. Mol Biol Cell 13: 2598–2606.P. LudovicoF. RodriguesA. AlmeidaMT SilvaA. Barrientos2002Cytochrome c release and mitochondria involvement in programmed cell death induced by acetic acid in Saccharomyces cerevisiae.Mol Biol Cell1325982606
  18. 18. Manon S, Chaudhuri B, Guerin M (1997) Release of cytochrome c and decrease of cytochrome c oxidase in Bax-expressing yeast cells, and prevention of these effects by coexpression of Bcl-xL. FEBS Lett 415: 29–32.S. ManonB. ChaudhuriM. Guerin1997Release of cytochrome c and decrease of cytochrome c oxidase in Bax-expressing yeast cells, and prevention of these effects by coexpression of Bcl-xL.FEBS Lett4152932
  19. 19. Wissing S, Ludovico P, Herker E, Buttner S, Engelhardt SM, et al. (2004) An AIF orthologue regulates apoptosis in yeast. J Cell Biol 166: 969–974.S. WissingP. LudovicoE. HerkerS. ButtnerSM Engelhardt2004An AIF orthologue regulates apoptosis in yeast.J Cell Biol166969974
  20. 20. Pozniakovsky AI, Knorre DA, Markova OV, Hyman AA, Skulachev VP, et al. (2005) Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast. J Cell Biol 168: 257–269.AI PozniakovskyDA KnorreOV MarkovaAA HymanVP Skulachev2005Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast.J Cell Biol168257269
  21. 21. Fannjiang Y, Cheng WC, Lee SJ, Qi B, Pevsner J, et al. (2004) Mitochondrial fission proteins regulate programmed cell death in yeast. Genes Dev 18: 2785–2797.Y. FannjiangWC ChengSJ LeeB. QiJ. Pevsner2004Mitochondrial fission proteins regulate programmed cell death in yeast.Genes Dev1827852797
  22. 22. Kroemer G (1997) Mitochondrial implication in apoptosis. Towards an endosymbiont hypothesis of apoptosis evolution. Cell Death Differ 4: 443–456.G. Kroemer1997Mitochondrial implication in apoptosis. Towards an endosymbiont hypothesis of apoptosis evolution.Cell Death Differ4443456
  23. 23. Haussmann P, Zimmermann FK (1976) The role of mitochondria in carbon catabolite repression in yeast. Mol Gen Genet 148: 205–211.P. HaussmannFK Zimmermann1976The role of mitochondria in carbon catabolite repression in yeast.Mol Gen Genet148205211
  24. 24. Sesaki H, Southard SM, Yaffe MP, Jensen RE (2003) Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane. Mol Biol Cell 14: 2342–2356.H. SesakiSM SouthardMP YaffeRE Jensen2003Mgm1p, a dynamin-related GTPase, is essential for fusion of the mitochondrial outer membrane.Mol Biol Cell1423422356
  25. 25. Hell K, Herrmann JM, Pratje E, Neupert W, Stuart RA (1998) Oxa1p, an essential component of the N-tail protein export machinery in mitochondria. Proc Natl Acad Sci U S A 95: 2250–2255.K. HellJM HerrmannE. PratjeW. NeupertRA Stuart1998Oxa1p, an essential component of the N-tail protein export machinery in mitochondria.Proc Natl Acad Sci U S A9522502255
  26. 26. Jia L, Dienhart M, Schramp M, McCauley M, Hell K, et al. (2003) Yeast Oxa1 interacts with mitochondrial ribosomes: the importance of the C-terminal region of Oxa1. Embo J 22: 6438–6447.L. JiaM. DienhartM. SchrampM. McCauleyK. Hell2003Yeast Oxa1 interacts with mitochondrial ribosomes: the importance of the C-terminal region of Oxa1.Embo J2264386447
  27. 27. Meunier JR, Choder M (1999) Saccharomyces cerevisiae colony growth and ageing: biphasic growth accompanied by changes in gene expression. Yeast 15: 1159–1169.JR MeunierM. Choder1999Saccharomyces cerevisiae colony growth and ageing: biphasic growth accompanied by changes in gene expression.Yeast1511591169
  28. 28. Kroemer G, Pouyssegur J (2008) Tumor cell metabolism: cancer's Achilles' heel. Cancer Cell 13: 472–482.G. KroemerJ. Pouyssegur2008Tumor cell metabolism: cancer's Achilles' heel.Cancer Cell13472482
  29. 29. Schulz TJ, Thierbach R, Voigt A, Drewes G, Mietzner B, et al. (2006) Induction of oxidative metabolism by mitochondrial frataxin inhibits cancer growth: Otto Warburg revisited. J Biol Chem 281: 977–981.TJ SchulzR. ThierbachA. VoigtG. DrewesB. Mietzner2006Induction of oxidative metabolism by mitochondrial frataxin inhibits cancer growth: Otto Warburg revisited.J Biol Chem281977981
  30. 30. Vaughn AE, Deshmukh M (2008) Glucose metabolism inhibits apoptosis in neurons and cancer cells by redox inactivation of cytochrome c. Nat Cell Biol 10: 1477–1483.AE VaughnM. Deshmukh2008Glucose metabolism inhibits apoptosis in neurons and cancer cells by redox inactivation of cytochrome c.Nat Cell Biol1014771483
  31. 31. Cardenas-Navia LI, Mace D, Richardson RA, Wilson DF, Shan S, et al. (2008) The pervasive presence of fluctuating oxygenation in tumors. Cancer Res 68: 5812–5819.LI Cardenas-NaviaD. MaceRA RichardsonDF WilsonS. Shan2008The pervasive presence of fluctuating oxygenation in tumors.Cancer Res6858125819
  32. 32. Hammond EM, Giaccia AJ (2004) The role of ATM and ATR in the cellular response to hypoxia and re-oxygenation. DNA Repair (Amst) 3: 1117–1122.EM HammondAJ Giaccia2004The role of ATM and ATR in the cellular response to hypoxia and re-oxygenation.DNA Repair (Amst)311171122
  33. 33. Buttner S, Bitto A, Ring J, Augsten M, Zabrocki P, et al. (2008) Functional mitochondria are required for alpha-synuclein toxicity in aging yeast. J Biol Chem 283: 7554–7560.S. ButtnerA. BittoJ. RingM. AugstenP. Zabrocki2008Functional mitochondria are required for alpha-synuclein toxicity in aging yeast.J Biol Chem28375547560
  34. 34. Buttner S, Eisenberg T, Carmona-Gutierrez D, Ruli D, Knauer H, et al. (2007) Endonuclease G regulates budding yeast life and death. Mol Cell 25: 233–246.S. ButtnerT. EisenbergD. Carmona-GutierrezD. RuliH. Knauer2007Endonuclease G regulates budding yeast life and death.Mol Cell25233246