The Whi5 transcriptional repressor is a negative regulator of G1 cell cycle progression in Saccharomyces cerevisiae and is functionally equivalent to the Retinoblastoma (Rb) tumor suppressor protein in mammals. In early G1, Whi5 binds to and inhibits SBF (Swi4/Swi6) transcriptional complexes. At Start, Cln:Cdc28 kinases phosphorylate and inactivate Whi5, causing its dissociation from SBF promoters and nuclear export, allowing activation of SBF transcription and entry into late G1. In an analysis of Whi5 phosphorylation, we found that 10 of the 12 putative CDK phosphorylation sites on Whi5 were occupied in vivo in asynchronously growing cells. In addition, we identified 6 non-CDK Whi5 phosphorylation sites. Whi5 CDK and non-CDK phosphorylation mutants were functional and able to rescue the small cell size of whi5Δ cells. However, the Whi5 CDK mutant with all 12 putative CDK sites changed to alanine causes a dramatic cell cycle phenotype when expressed with a Swi6 CDK phosphorylation mutant. Mutational analysis of Whi5 determined that only four C-terminal CDK sites were necessary and sufficient for Whi5 inactivation when Swi6 CDK sites were also mutated. Although these four Whi5 CDK sites do not wholly determine Whi5 nuclear export, they do impact regulation of cell size. Taken together, these observations begin to dissect the regulatory role of specific phosphorylation sites on Whi5.
Citation: Wagner MV, Smolka MB, de Bruin RAM, Zhou H, Wittenberg C, Dowdy SF (2009) Whi5 Regulation by Site Specific CDK-Phosphorylation in Saccharomyces cerevisiae. PLoS ONE4(1): e4300. https://doi.org/10.1371/journal.pone.0004300
Editor: Mikhail V. Blagosklonny, Ordway Research Institute, United States of America
Received: December 15, 2008; Accepted: December 22, 2008; Published: January 28, 2009
Copyright: © 2009 Wagner et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: M.V.W. was funded by the NIH Cancer Cell Biology Training grant (CA067754) and the NIH Basic Clinical Genetics Training grant (GM008666). This work was supported by the Howard Hughes Medical Institute (S.F.D.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Cell proliferation is a tightly regulated process where extracellular signals and intracellular checkpoints are integrated to control cell growth and division. When conditions for cell division are favorable, cells commit to enter the cell cycle during G1 phase at an irreversible transition termed Start in yeast and the Restriction Point in mammalian cells , . In yeast, environmental nutrient availability regulates accumulation of cellular mass to a critical cell size before Start . In mammalian cells, growth factor signaling determines passage through the Restriction Point that is also presumed to be partially responsive to cell size and mass. Failure to properly regulate cell cycle entry can result in abnormal division and lead to development of neoplastic disease . The molecular events determining commitment to cell division in both yeast and mammalian cells involve regulated transcription of groups of genes required for cell cycle progression , . In yeast, expression of genes at Start depends on SBF and MBF transcription factor complexes; each consists of the transcriptional coactivator Swi6 and a DNA binding protein, Swi4 or Mbp1, respectively . In general, SBF controls transcription of cell cycle regulatory genes, while genes involved in DNA synthesis and repair are MBF targets . Loss of both Swi4 and Mbp1 results in a permanent G1 arrest and cell lethality, demonstrating the essential function of these transcription factor complexes .
In early G1, SBF complexes bind target promoters, but due to binding by the Whi5 transcriptional repressor, do not initiate transcription until Start , . The cyclin Cln3 is an activator of cell cycle progression and ensures proper timing of transcription once cells reach a critical cell size , . Cells lacking CLN3 have a large cell size phenotype and are delayed in transcriptional activation and passage through Start –. Whi5 is a key target of Cln3-Cdc28 cyclin dependent kinase (CDK) in transcriptional activation of SBF. Phosphorylation of Whi5 and SBF complexes by Cln3-Cdc28 results in Whi5 dissociation from SBF promoters and nuclear export, allowing for induction of SBF transcription and cell cycle entry , . Consequently, cells lacking WHI5 initiate SBF transcription prematurely, driving cells past Start and resulting in a small cell size . In addition to Whi5, Swi4 and Swi6 are also known targets of CDK phosphorylation and genetic studies implicate Swi6 as a critical target of Cln3 . However, mutation of CDK phosphorylation sites of Swi4 or Swi6 does not interfere with timing of SBF transcription –. Interestingly, a Whi5 CDK phosphorylation mutant causes cell lethality when expressed in a Swi6 CDK phosphorylation mutant background, suggesting that CDK phosphorylation of Whi5 and Swi6 is redundant for transcriptional activation . Swi6 is also subject to inhibitory phosphorylation. During the late G1 to early S-phase transition, Swi6 is phosphorylated by cyclin Clb6-Cdc28 on Ser-160, changing Swi6 cellular localization from nuclear to cytoplasmic , . This serves to inactivate SBF transcription outside of G1. It is not currently known what mechanisms repress MBF in early G1 or activate MBF at Start, although the MBF target Nrm1 is responsible for repression of MBF upon exit from G1 .
A functionally similar system of transcriptional regulation occurs in mammalian cells. The E2F family of transcription factors control expression of a suite of E2F target genes essential for late G1 cell cycle progression and DNA synthesis . In early G1, E2Fs are bound and repressed by the Retinoblastoma Tumor Suppressor protein (Rb), a functional analogue of Whi5 , . At the restriction point, activation of Cyclin E-Cdk2 kinase complexes results in Rb hyper-phosphorylation and inactivation, allowing for cell cycle-dependent E2F transcription . Studies of Rb regulation by phosphorylation show Rb is phosphorylated at low levels, or hypo-phosphorylated, when bound to E2Fs in early G1 . Additionally, it has been proposed by some groups that specific CDK sites on Rb are utilized for inactivation , , while others suggest a model where a critical threshold of phosphorylation on any of Rb's 16 CDK sites induces inactivation , . However, the mechanistic understanding of Rb regulation by phosphorylation remains unclear.
Due to the conserved regulatory pathways controlling G1 transcription between yeast and mammalian cells, investigation of regulation by phosphorylation of Whi5 in yeast could provide insight into mammalian G1 cell cycle and Rb regulation. In an analysis of Whi5 phosphorylation, we determined that Whi5 is also found hypo-phosphorylated in early G1, but that hypo-phosphorylation is not required for Whi5 function. Additionally, we identify four specific CDK sites in Whi5 that are critical for Whi5 inactivation and the regulation of cell size when Swi6 phosphorylation is also prevented. These results demonstrate that regulation of Whi5 by phosphorylation relies on specific CDK sites.
Materials and Methods
Yeast Culture and Strains
Cells were grown in standard media containing yeast extract, peptone, and 2% glucose. For induction experiments, cells were grown overnight in media containing 2% raffinose then inoculated into 2% galactose media or galactose plates. A 13x-Myc tag was appended to WHI5 using the method of Longtine et al . The WHI5 promoter construct derived from 545 base pairs 5′ of the WHI5 open reading frame was PCR amplified from genomic DNA and cloned into the pRS413 vector .
See Table 1 for a list of yeast strains used in this study.
Cells expressing GAL1-WHI5-3xHA or GAL1-WHI5-12Ala-3xHA were grown in raffinose then induced with galactose for ten hours. Cells from twelve liters of culture were harvested by centrifugation and drop frozen in liquid nitrogen. Cells were broken with a Waring blender in liquid nitrogen. Resulting powder was resuspended in RIPA buffer with protease and phosphatase inhibitors. Cells debris was pelleted by high-speed centrifugation. Immunoprecipitation with HA affinity resin (Roche) was performed overnight. Immunoprecipitates were washed and boiled in SDS-sample buffer then resuspended in RIPA buffer, diluting the SDS to 1%, before a second immunoprecipitation was performed. Samples from the double immunoprecipitation were separated by 10% SDS-PAGE and stained with Coomassie blue.
After in-gel digestion, phosphopeptides were purified by IMAC as previously described . Purified phosphopeptides were analyzed by µLC-ESI-MS/MS on a Thermo Finnigan LTQ quadrupole ion trap mass spectrometer as described . For data analysis, SEQUEST (version 3.4 beta 2) program running on a Sorcerer system (SageN, San Jose, CA) was used for peptide identification. Database search was performed using the budding yeast database. The following variable modifications were considered: +80 Da (phosphorylation) for serine, threonine and tyrosine residues; +16 Da (oxidation) for methionine residues. Up to 4 variable modifications were allowed per peptide and peptide mass tolerance used was 3 Da. A semi-tryptic restriction was applied, and only the top-matched peptides with a probability score above 0.9 were subsequently considered for close inspection. Each MS/MS spectrum that led to phosphopeptide identification was manually verified to confirm all significant ions were accounted for, and then validated.
Protein Analysis and Phosphatase Treatment
Cells were broken with glass beads in 50 mM Tris, pH 8, 150 mM NaCl, 2 mM EDTA, 10% glycerol, 0.2% NP-40 lysis buffer containing protease (PMSF, aprotinin, leupeptin, benzamidine) and phosphatase inhibitors (Sigma cocktails 1 and 2). Immunoprecipitation was performed with anti-Myc antibody (Santa Cruz) and protein G beads (Zymed) or anti-HA conjugated beads (Roche). After washing with lysis buffer without phosphatase inhibitors, and 50 mM Tris, pH 8, 50 mg/mL BSA, 25 uM DTT buffer, immunoprecipitates were incubated with lambda protein phosphatase (New England Biolabs) in supplied buffer for 1 hour at 30 degrees . Analysis of protein migration utilized 10% SDS-PAGE run for 10–12 cm.
Cell Size Measurements
Cell size analysis was performed on asynchronous cultures during log-phase growth using a Coulter Z2 Particle Cell Analyzer (Beckman-Coulter). Cultures were briefly sonicated before analysis. Cell size distribution was analyzed with the Z2 AccuComp software (Beckman-Coulter).
Fluorescence and differential interference contrast microscopy was performed on live cells with a DeltaVision RT Microscope (Applied Precision Life Science) using a 100× oil objective. Image processing was done with ImageJ software and fluorescent and DIC images were overlaid using Adobe Photoshop.
Cells in log phase growth were washed, resuspended in fresh media containing 5 µg/mL alpha factor (Sigma), and incubated for three hours at 30 degrees. To release, cells were washed twice with cold media then inoculated into warm media. At indicated time points, samples were taken, cooled with ice, sonicated, then analyzed for budding index.
Whi5 is phosphorylated on 16 CDK and non-CDK sites in vivo
Whi5 contains 12 putative CDK phosphorylation sites typified by S/T-P(-X-B) and phosphorylation by Cln:Cdk activity has been shown to be important in Whi5 inactivation , . de Bruin et al. had previously determined that CDK sites 2, 4, 5, 10 and 12 were phosphorylated in vivo from samples of late G1 cells . To identify Whi5 phosphorylation sites in vivo in a comprehensive manner, we performed mass spectrometry analysis of Whi5. Wild type Whi5 containing a 3× HA C-terminal tag under the control of the GAL1 promoter was immunopurified from asynchronously growing cells, digested with trypsin, and phosphopeptides were purified by IMAC resin liquid chromatography enrichment then analyzed by mass spectrometry. Ten of the 12 putative Whi5 CDK phosphorylation sites, numbered 3–12, were identified as being phosphorylated in asynchronous cells (Fig. 1), while phosphorylation of sites 1 and 2 were not detected by our methods. Because de Bruin et al. verified phosphorylation of CDK site 2 , and it is possible CDK site 1 is phosphorylated but not detected by our methods, we utilize a Whi5-12Ala phosphorylation mutant in our following studies. Surprisingly, in addition to CDK sites, we also identified 6 other non-CDK serine and threonine residues phosphorylated on Whi5, labeled A to F (Fig. 1). We note that 5 of the 6 non-CDK phosphorylation sites contain an acidic residue distal to the phosphorylated S/T. Mass spectrometry analysis of 3× HA C-terminal tagged mutant Whi5 with all 12 CDK sites changed to alanine (Whi5-12Ala-3xHA) maintained phosphorylation of these 6 non-CDK sites. In total, we identified 16 phosphorylation sites on Whi5 in asynchronously growing cells.
Whi5 is phosphorylated at all stages of the cell cycle
We next analyzed the cell cycle dependency of Whi5 phosphorylation, utilizing a strain containing a C-terminal 3x-Myc tag integrated at the WHI5 locus. In lysates from asynchronously growing cells, Whi5-13xMyc protein migrates as a doublet (Fig. 2A). However, in cells arrested in G1 phase by mating pheromone, where CDK activity is inhibited by Far1 , , Whi5-13xMyc migrates as a single, faster migrating band. In contrast, in cells arrested in M phase by nocodazole, where CDK activity is high, the slower migrating species was enriched. To test whether the difference in migration of the Whi5 bands was due to phosphorylation, immunoprecipitates of Whi5-13xMyc were treated with lambda phosphatase (Fig. 2B). Upon phosphatase treatment, both the slower and faster migrating species of Whi5 from asynchronous cells collapsed into a third, fastest migrating un-phosphorylated band. Phosphatase treatment of Whi5-13xMyc from mating pheromone and nocodazole-arrested cells both induced appearance of the un-phosphorylated band. Thus, Whi5 can be separated into three species: the slowest migrating form of Whi5, designated hyper-phosphorylated; the middle form, designated hypo-phosphorylated; and the fastest form, designated un-phosphorylated. We note that the un-phosphorylated form of Whi5 was not found at any cell cycle stage or even in mating pheromone arrested cells. In addition, we observe that Whi5 from mating pheromone arrested cells with inhibited CDK activity shifted downward after phosphatase treatment, suggesting the presence of non-CDK phosphorylation events.
(A) Cultures of strains expressing Whi5-13xMyc from the endogenous locus were arrested in G1 phase with alpha factor or in metaphase with nocodazole. Western blot of Whi5-13xMyc shows two different migrating forms, indicated by arrows. (B) Western blot of immunopurified Whi5-13xMyc treated with or without lambda phosphatase. Three different migrating forms indicated by arrows represent unphosphorylated, hypo-phosphorylated, and hyper-phosphorylated Whi5. (C) Strains carrying CEN plasmids of phosphorylation mutants of Whi5-13xMyc under the GAL1 promoter were grown in galatcose before immunopurification. Whi5-WT, non CDK mutant 6Ala, CDK mutant 12Ala, and CDK/non-CDK mutant 18Ala were treated with phosphatase as in B. Arrows indicate migration of three different phosphorylation forms.
The phosphorylation status of Whi5 mutants was also analyzed by phosphatase treatment. Wild type Whi5 or phosphorylation mutants containing a C-terminal 13xMyc tag were expressed from the GAL1 promoter on CEN plasmids. In samples of asynchronous cells, mutation of the 6 non-CDK sites (A–F) to alanine did not effect the migration of Whi5 compared to wild type Whi5 (Fig. 2C). Consistent with the mass spec analysis, mutation of Whi5 CDK sites 1–12 to alanine resulted in a single hypo-phosphorylated band that increased mobility after phosphatase treatment. Since both Whi5-6Ala and Whi5-12Ala were found phosphorylated, the non-CDK sites A–F are not essential for CDK site phosphorylation, and vice versa. Finally, when all 12 CDK and 6 non-CDK sites were mutated to alanine in Whi5-18Ala, migration did not change upon phosphatase treatment. In addition, in vivo orthophosphate labeling verified that both Whi5-6A and Whi5-12Ala are phosphorylated, although to a lesser extent than wild type Whi5, while phosphorylation of Whi5-18Ala was negligible (data not shown).
Functional Analysis of Whi5 phosphorylation mutants
whi5Δ cells exhibit a small cell size phenotype . To assess the functionality of Whi5 phosphorylation mutants, we assayed for their ability to rescue whi5Δ small size (Fig. 3A). Wild type parent strain BY4741 had a mean cell size distribution of 48.2 fL, while the mean size of whi5Δ cells was 36.2 fL in this experiment. Introduction of wild type WHI5 on a CEN plasmid under its native promoter into whi5Δ cells rescues the small cell size defect and returned the culture to a mean cell size of 53.2 fL. Expression of WHI5-12Ala also rescued the small cell size phenotype, with a mean cell size of 53.3 fL. Importantly, expression of WHI5-12Ala from its own promoter did not result in a larger cell size than WHI5-WT. This suggests CDK phosphorylation of Whi5 is not essential for SBF activation or timing of Start, as loss of Whi5 CDK phosphorylation does not delay cell cycle progression. Mutants in the non-CDK phosphorylation sites of Whi5 were also assayed for ability to rescue whi5Δ cell size. In this experiment, where cells are grown in synthetic media, parent strain BY4741 had a mean cell size of 35.3 fL, while whi5Δ cells were 31.5 fL. Expression of WHI5 wild type, WHI5-12Ala (CDK), WHI5-6Ala (non-CDK), or WHI5-18Ala (CDK and non-CDK) from the inducible MET3 promoter on CEN plasmids was able to rescue the small cell size of whi5Δ mutants, with mean cell sizes of 42.1, 43.5, 43.2 and 42.4 fL, respectively (Fig. 3B). We note that the slightly larger cell size observed is likely due to constitutive expression of Whi5 from the MET3 promoter, and was also observed by de Bruin et al . These observations indicate that in wild type cells, phosphorylation of Whi5 on CDK sites or non-CDK sites is not essential for regulation of cell size. In addition, it has previously been shown that Whi5 overexpression in cln3Δ cells results in permanent cell cycle arrest in G1 prior to Start . Similar to wild type Whi5, overexpression of Whi5-12Ala, Whi5-6Ala, or Whi5-18Ala phosphorylation mutants from the GAL1 promoter on CEN plasmids caused lethality of cln3Δ cells (Fig. 3C), suggesting that hypo-phosphorylation of either CDK or non-CDK sites is not required for Whi5 to antagonize Cln3 activity.
(A) Size analysis of whi5Δ cells with CEN plasmids expressing Whi5-WT or Whi5-12Ala (CDK mutant) from the WHI5 promoter, a 545 bp fragment from sequence directly 5′ of the WHI5 open reading frame. (B) Size analysis of whi5Δ cells expressing Whi5-WT, Whi5-12Ala (CDK mutant), Whi5-6Ala (non CDK mutant), or Whi5-18A (CDK and non-CDK mutant) from the MET3 promoter on a CEN plasmid. (C) Wild type cells or cln3Δ cells with empty vector or CEN plasmid constructs of Whi5-WT, Whi5-12Ala, Whi5-6Ala, or Whi5-18Ala under control of the GAL1 promoter. Cells were spotted in serial five fold dilutions on glucose or galactose media and incubated 48 hours. (D) swi6Δ [SWI6-SA4] cells bearing either empty vector or CEN plasmid constructs of Whi5-WT, Whi5-12Ala, Whi5-6Ala, or Whi5-18Ala under the GAL1 promoter. Cells were spotted in serial five fold dilutions on glucose or galactose media and incubated 48 hours.
Specific CDK phosphorylation sites of Whi5 are required for function
Expression of various Whi5 phosphorylation mutants in wild type cells does not result in a dramatic phenotype , . However, overexpression of Whi5-12Ala in cells that harbor the Swi6-SA4 mutant lacking four CDK sites results in a severe growth defect , suggesting that phosphorylation of either Whi5 or Swi6 is required for SBF activation and cell cycle promotion. To test if the non-CDK sites of Whi5 are similarly essential for SBF activation in the presence of Swi6-SA4, Whi5 mutants containing alanine substitutions for the 6 non-CDK sites (Whi5-6Ala), and all CDK and non-CDK sites (Whi5-18Ala) were tested in this assay. In cells expressing wild type Swi6, all of the WHI5 constructs, WHI5-WT, WHI5-12Ala, WHI5-6Ala, or WHI5-18Ala expressed from the GAL1 promoter on CEN plasmids, showed no change in growth rate as detected in this assay (data not shown). However, in combination with SWI6-SA4, WHI5-12Ala and WHI5-18Ala cells were inviable, whereas cells expressing WHI5-WT or WHI5-6Ala were viable (Fig. 3D). Thus, phosphorylation of Whi5 non-CDK sites is not necessary for SBF activation in a SWI6 or SWI6-SA4 genetic background. We are currently unable to ascribe a biological function for phosphorylation of Whi5 at the 6 non-CDK sites.
Although phosphorylation of multiple Whi5 CDK sites was detected in vivo (Fig. 1 and de Bruin et al.) , it is not known whether specific CDK sites or a threshold amount of phosphorylation determines Whi5 inactivation and dissociation from SBF. To test these two competing hypotheses of Whi5 inactivation, we performed a mutational analysis of Whi5 CDK sites and assayed for genetic interaction with the SWI6-SA4 allele (Fig. 4). Consistent with previous reports , none of the Whi5 CDK mutants assayed caused growth defects when induced in cells expressing wild type Swi6 (data not shown). Similar to WHI5-12Ala, expression of WHI5-7Ala (CDK sites 2, 3, 5, 8, 9, 10, and 12 mutated to alanine)  caused lethality in combination with SWI6-SA4 (Fig. 4, lines 2 & 3). Based on a preliminary survey of phosphorylation site mutants, we initially focused our attention on the C-terminal CDK phosphorylation sites. Four C-terminal CDK sites of Whi5 (8, 9, 10, and 12) as a group were necessary and sufficient for viability with Swi6-SA4. When WHI5-4Ala, with the four sites mutated to alanine, is expressed in combination with SWI6-SA4, cells were inviable (Fig. 4, line 4). In contrast, when WHI5-8Ala1, where the other 8 CDK sites in Whi5 are mutated to alanine, is expressed with SWI6-SA4, cells were viable (Fig. 4, line 5). This demonstrates that CDK sites 8, 9, 10, and 12 are specifically required for Whi5 inactivation in this assay. To rule out the possibility that phosphorylation of any four CDK sites was sufficient to inactivate Whi5, several constructs having four or more wild type CDK sites were also analyzed. For example, when four N-terminal CDK sites (2, 3, 4, and 5) are wild type while the other eight CDK sites are alanine in WHI5-8Ala2 (Fig. 4, line 6), the SWI6-SA4 cells were still inviable, suggesting that the four N-terminal sites were not sufficient to allow for viability.
(A) swi6Δ [SWI6-SA4] cells expressing Whi5-WT or Whi5 phosphorylation mutants from the GAL1 promoter. Cells were spotted in serial 5 fold dilutions on galactose media and incubated 48 hours. The table indicates the CDK sites of Whi5 that were left wild type (S/T) or mutated to alanine (A) for each mutant construct. (B) Schematic of the Whi5 protein showing relative location of CDK sites, numbered 1–12.
Additional analysis of CDK sites 8, 9, 10, and 12 was performed to assess their function as single sites, pairs of sites, or in groups of three. Mutation of site 10 or 12 alone, or pairs of sites (10 and 12; or 8 and 9) to alanine, while leaving the other CDK sites wild type did not affect cell growth (Fig. 4, lines 7–10). This demonstrates that no single C-terminal CDK site or pair of sites is necessary for viability. Conversely, mutating all other CDK sites to alanine, leaving only single CDK sites 10 or 12, or pairs of sites (10 and 12; or 8 and 9) wild type did not permit cell growth (Fig. 4, lines 11–14). This indicates that phosphorylation of a single site or pair of sites is not sufficient to support cell viability. Interestingly, when three CDK sites (8, 9, and 12; or 8, 9, and 10) were left wild type, they were sufficient to allow growth (Fig. 4, line 15), indicating 3 out of the 4 C-terminal CDK sites are sufficient for viability. Because these mutants with only three functional CDK sites are viable, the specific C-terminal CDK sites are adequate to promote Whi5 inactivation. However, Whi5 regulation by phosphorylation is complex in that some combinations of C-terminal mutations with other CDK sites influenced viability (Fig. 4, lines 17 & 18, lines 19 & 20). While CDK sites 8, 9, 10, and 12 are both necessary and sufficient for viability in combination with Swi6-SA4, supporting a model of specific sites utilized for inactivation, other CDK sites are able to support inactivation in certain combinations.
Localization of Whi5 CDK mutants
In wild type cells, Whi5 localization is nuclear in early G1, then exported to the cytoplasm just prior to budding . Since Swi6-SA4 is constitutively localized in the nucleus , we hypothesized that Whi5 CDK mutants that caused lethality with Swi6-SA4 might also have defects in nuclear export. Localization of GFP tagged Whi5 was examined in wild type cells. As previously reported , Whi5-WT-GFP is nuclear in unbudded cells and cells just exiting mitosis, but is cytoplasmic in budded cells (Fig. 5A). However, Whi5-12Ala-GFP was localized to the nucleus in both unbudded and budded cells (Fig. 5B), confirming that CDK phosphorylation is required for export . The localization of Whi5-4Ala-GFP, containing mutations of CDK sites 8, 9, 10, and 12, is nuclear in unbudded cells (Fig. 5C), but is cytoplasmic in only 16% of budded cells (Fig. 5C, 2nd panel and Fig. 5E). Whi5-8Ala1-GFP, with CDK sites 8, 9, 10, and 12 wild type, is nuclear in unbudded cells, and is cytoplasmic 35% of budded cells (Fig. 5D and 5E). The localization of Whi5-WT-GFP and CDK mutants was also examined in SWI6-SA4 cells, but was not different than localization observed in wild type cells (Fig. 5E). These observations suggest that in wild type or SWI6-SA4 cells, Whi5 C-terminal CDK sites 8, 9, 10, and 12 influence localization, but are not entirely necessary or sufficient for wild type localization.
(A) Cultures containing GAL1-WHI5-WT-GFP, (B) GAL1-WHI5-12Ala-GFP, (C) GAL1-WHI5-4Ala-GFP, or (D) GAL1-WHI5-8Ala-GFP on a CEN plasmid were grown in galactose media and photographed for Whi5-GFP fluorescence in unbudded and budded cells. Representative cells are shown. (E) Graph of Whi5 localization in budded cells, showing the percentage of budded cells with cytoplasmic Whi5 in either wild type BY4741 or swi6Δ [SWI6-SA4] cells.
MSN5 is required for Whi5 nuclear export
Swi6 nuclear export is dependent on the karyopherin Msn5 . Because Swi6 and Whi5 interact , , we examined whether Whi5 nuclear export was also Msn5 dependent. In msn5Δ cells, GFP tagged Whi5-WT was constitutively nuclear, even in budded cells (Fig. 6A). Whi5-WT was also nuclear in swi6Δ cells (Fig. 6B), suggesting that Swi6 is also involved in Whi5 nuclear export. Msn5 overexpression from the GAL1 promoter was not sufficient to induce ectopic nuclear export of Whi5-WT, as its localization remains nuclear in unbudded cells (Fig. 6C). Additionally, GAL1-MSN5 was unable to induce export of Whi5-12Ala and localization remained constitutively nuclear (Fig. 6C). Similarly, GAL1-MSN5 was unable to rescue the growth defect of GAL1-WHI5-12A SWI6-SA4 co-expressing cells (data not shown). Because Msn5 is known to export phosphoproteins –, we examined phosphorylation of Whi5 in msn5Δ and swi6Δ cells (Fig. 6D). Immunoprecipitation and phosphatase treatment revealed that Whi5-WT was both hypo-phosphorylated and hyper-phosphorylated in msn5Δ and swi6Δ cells similar to wild type cells, indicating that the failure to export is not due to lack of Whi5 CDK phosphorylation.
(A) msn5Δ cells, or (B) swi6Δ cells containing GAL1-WHI5-WT-GFP CEN plasmid were grown in galactose media and photographed for Whi5-GFP fluorescence in unbudded and budded cells. Representative cells are shown. (C) Cells expressing MSN5 from a GAL1 promoter integrated at the endogenous locus contain either GAL1-WHI5-WT-GFP or GAL1-WHI5-12Ala-GFP CEN plasmids. Cells were grown and photographed as above. (D) Anti-Myc western blot of immunopurified Whi5-WT-13xMyc from wild type cells, msn5Δ, or swi6Δ cells untreated or treated with lambda phosphatase. Arrows indicate presence of three different migrating species.
Whi5 C-terminal CDK sites affect cell size
To determine if the four C-terminal CDK sites of Whi5 affect normal cell cycle progression, WHI5-WT and mutant alleles were expressed from the WHI5 promoter in combination with SWI6-SA4. In contrast to cells overexpressing Whi5-12Ala from the GAL1 promoter (as in Fig. 4), cells expressing Whi5-12Ala and Swi6-SA4 from their own promoters at physiologic levels are viable and grow at rates similar to cells expressing Whi5-WT and Swi6-WT (Fig. 7A) and have normal cell cycle profiles (data not shown). When cells were synchronized in G1 with mating pheromone and released, WHI5-12A SWI6-SA4 cells budded at the same time and rate as WHI5-WT SWI6-WT cells (Fig. 7B). Although the cells displayed no overt growth defect, WHI5-12Ala SWI6-SA4 co-expressing cells were large (Fig. 7C). The mean cell volume (fL) of cells co-expressing Whi5-12Ala and Swi6-SA4 was 67.6 fL, 40% larger than cells expressing wild type WHI5 and SWI6 with a mean of 48.2 fL, indicating that phosphorylation of either Swi6 or Whi5 is necessary for regulation of cell size. Cells with WHI5-WT and SWI6-SA4, or cells with WHI5-12Ala SWI6-WT, were similar in size distribution to WHI5-WT SWI6-WT cells, demonstrating that the effect on cell size is not observed in cells with either mutant alone. We also analyzed the cell size distribution of Whi5 CDK mutants. When expressed with Swi6-WT, Whi5-4Ala and Whi5-8Ala1 cells were similar in size to Whi5-WT and Whi5-12Ala (data not shown). In contrast, cells expressing Swi6-SA4 with Whi5-4Ala (CDK sites 8, 9, 10, 12 Ala) were larger in size, with a mean size of 62.0 fL, with a similar distribution as Swi6-SA4 Whi5-12Ala cells (Fig. 7C). Conversely, the size distribution of Swi6-SA4 cells with Whi5-8Ala1 (CDK sites 8, 9, 10, 12 WT), with a mean size of 54.6, was similar to that of cells containing both wild type alleles. These results demonstrate that in combination with Swi6-SA4, phosphorylation of Whi5 C-terminal CDK sites (8, 9, 10, and 12) is essential for maintenance of cell size.
(A) Growth rates measured by OD600 of swi6Δwhi5Δ cells expressing Swi6-WT or Swi6-SA4, and Whi5-WT or Whi5-12Ala from the 545 bp WHI5 promoter fragment on CEN plasmids. (B) Budding index of the same strains as in A. Cells were synchronized with alpha factor and released into fresh media. Budded cells were counted every 10 minutes. (C) Cell size distribution analysis wild type cells (BY4741), whi5Δ cells, and swi6Δ cells compared to swi6Δwhi5Δ cells expressing Swi6-WT or Swi6-SA4, and Whi5-WT, Whi5-12Ala, Whi5-4Ala, or Whi5-8Ala from the 545 bp WHI5 promoter fragment on CEN plasmids.
Whi5 CDK and non-CDK phosphorylation
Whi5 is a negative regulator of G1 cell cycle progression that is inactivated by CDK phosphorylation at Start , . Whi5 contains 12 putative CDK phosphorylation sites. However, it has remained unclear if specific CDK phosphorylations sites on Whi5 are utilized for inactivation or if a critical phosphorylation threshold of randomly distributed CDK sites induces inactivation. Here we found that Whi5 is phosphorylated in vivo on 10 CDK sites and 6 novel, non-CDK sites. We also observed that Whi5 is found hypo-phosphorylated at all stages of the cell cycle, including early G1. We did not observe un-phosphorylated Whi5, even in mating pheromone arrested cells. Five out of 6 of the non-CDK sites are followed by acidic residues and conform to the consensus sequences for casein kinase I or casein kinase II (CKA1/2) , which is known to target transcription factors and RNA polymerases . We also found that hypo-phosphorylation is not required for the repressive function Whi5 as whi5Δ cells expressing Whi5-18Ala (all CDK and non-CDK sites mutated to alanine) are normal in cell size and overexpression of Whi5-18Ala causes lethality in cln3Δ cells. Further experiments are needed to explore the biological function of Whi5 non-CDK phosphorylation and identify the kinase(s) responsible.
In addition to showing that Whi5 hypo-phosphorylation is not essential for inhibitory function, this analysis shows that phosphorylation is neither essential for Whi5 inactivation nor passage through Start. Whi5-12Ala expressed from the WHI5 promoter fails to effect cell size distribution, indicating that the timing of cell cycle entry is not significantly affected by the phosphorylation site mutations. Our results differ from those of de Bruin et al, who report that Whi5-7Ala expressed from the MET3 promoter caused a measurable defect in cell cycle progression, with cells inducing maximum SBF transcription at a ∼10% larger cell size . The difference in results may be due to strain background differences or the use of the heterologous promoter. Nevertheless, the reported effect of Whi5-7Ala was not as significant as would be predicted if Whi5 phosphorylation was essential for SBF activation.
Redundancy of Whi5 and Swi6 Phosphorylation
In contrast to the finding that Whi5 phosphorylation is not essential for cell size regulation at physiologic expression levels, phosphorylation of Whi5 and Swi6 becomes essential when Whi5 is overexpressed, as phosphomutants of these proteins synergize to cause lethality. Swi6 has 5 putative CDK phosphorylation sites; four of the five are in the N-terminal region, while the fifth CDK site is a part of the ankyrin repeat domain of Swi6. The SWI6-SA4 mutant created by Sidorova, et al., that contains mutations of the four N-terminal CDK sites, does not affect periodic transcription of SBF targets (data not shown) . While Swi6-SA4 alone does not have a significant cell cycle phenotype, Swi6-SA4 cells expressing GAL1-WHI5-12Ala are inviable and arrest as large, elongated, unbudded cells (data not shown) . We exploited this phenotype in a mutational analysis of Whi5 CDK sites to identify four C-terminal CDK sites of Whi5, sites 8, 9, 10, and 12, that are necessary and sufficient to prevent lethality when Whi5 is overexpressed in combination with Swi6-SA4. Importantly, Whi5 retaining four or more N-terminal CDK sites intact was not sufficient to prevent lethality. This demonstrates that specific CDK sites of Whi5 are required for inactivation, rather than simply a threshold number. Consequently, we can exclude a model similar to that adopted for proteins such as Sic1 that require a threshold number of CDK phosphorylations to induce inactivation without a requirement for specific sites of phosphorylation . These four phosphorylated residues of Whi5 may function to induce a conformational change or otherwise influence the interaction between Whi5 and SBF components. We conclude that phosphorylation of Whi5 and Swi6 is redundant, and that phosphorylation facilitates Whi5 inactivation.
CDK phosphorylation of Swi6 participates in the inactivation of Swi6 by inducing cytoplasmic localization , . Swi6 Serine-160 is adjacent to a nuclear localization sequence and inhibits nuclear import once phosphorylated. When Ser-160 is mutated to alanine, as it is in the Swi6-SA4 mutant, this results in constitutive nuclear localization of Swi6 . Whi5 is also exported from the nucleus upon CDK phosphorylation and a C-terminal phosphorylation mutant of Whi5 (sites 7–12 alanine) is largely nuclear . Analysis of Whi5-GFP localization shows four C-terminal sites (8, 9, 10, and 12) are involved in nuclear export, as Whi5-4A was unable to be completely exported to the cytoplasm in budded cells, but are not sufficient to control localization, as Whi5-8Ala (8, 9, 10, and 12 wild type) remained nuclear in two thirds of budded cells. This suggests that the four CDK sites implicated in Whi5 inactivation differ from the CDK sites required to induce Whi5 nuclear export, and highlights a function for CDK sites 7 and 11 in Whi5 export. These data also indicate that Whi5 dissociation from SBF and its subsequent export to the cytoplasm are distinct steps requiring phosphorylation of different CDK sites.
Despite the dramatic cell cycle phenotype of SWI6-SA4 cells when Whi5-12Ala is overexpressed, CDK phosphorylation of Whi5 and Swi6 is not essential for viability when Whi5 is expressed at physiologic levels. Cells expressing both Swi6-SA4 and Whi5-12Ala under control of their own promoters are completely viable, grow at normal rates, and enter the cell cycle after mating pheromone induced G1 arrest with normal kinetics. Their only apparent defect is a large cell size, suggesting that cells are delayed in passing Start when Whi5 and Swi6 cannot be phosphorylated. The four Whi5 CDK sites (8, 9, 10, and 12) identified in the overexpression assay with Swi6-SA4 are also necessary and sufficient to promote cell size regulation, as WHI5-4Ala SWI6-SA4 cells were equivalent in size to WHI5-12Ala SWI6-SA4 cells. Conversely, WHI5-8Ala1 (CDK sites 8, 9, 10, and 12 wild type) cells were similar in size to WHI5-WT when combined with SWI6-SA4. The effect of these mutants on cell size indicates that the four specific CDK sites are important for the timing of Whi5 inactivation, even at physiologic expression levels. However, these data also imply that Whi5 and Swi6 are not essential targets of Cln-Cdc28 for cell viability when expressed at physiologic levels, and phosphorylation of Swi6 or the four sites of Whi5 is only required for proper coordination of cell size with cell cycle entry. In the absence of available CDK sites on Whi5 or Swi6, cells must use a mechanism other than CDK phosphorylation to activate SBF transcription. It is possible that the cell cycle activator Bck2 plays a role under these conditions, given its ability to activate SBF transcription independently of Cdc28 activity , .
Parallels between Whi5 and Rb
The pathway of transcriptional activation in yeast is functionally similar to the mechanism of cell cycle entry in mammalian cells. Whi5 is a functional analog of the Retinoblastoma tumor suppressor protein that also represses transcription in early G1. Previous studies from our laboratory show that Rb bound to E2F transcription factors in early G1 is hypo-phosphorylated on CDK phosphorylation sites . Whi5 is similarly hypo-phosphorylated in early G1, but hypo-phosphorylation is not necessary for its repressive function. Further experiments are needed to determine the requirement for Rb hypo-phosphorylation in repression of E2F promoters. Additionally, phosphopeptide maps of hyper-phosphorylated Rb during late G1 are nearly identical to hypo-phosphorylated Rb found in early G1 , , indicating that individual CDK sites are targeted equally in Rb phosphorylation and suggesting specific sites do not have a significant function. This suggests a model of Rb regulation where inactivation by hyper-phosphorylation is achieved by phosphorylation of a threshold number of CDK sites . In contrast to that model for Rb, we identified specific CDK sites that function in Whi5 inactivation and regulation of cell size. Because the regulatory pathways of cell cycle entry are largely conserved between yeast and mammalian cells, the mechanism of regulation by specific phosphorylation observed for Whi5 suggests further investigation of Rb phosphorylation in mammalian systems is warranted.
We thank Mike Tyers (University of Toronto), Linda Breeden (Fred Hutchinson Cancer Research Center), and Rainer Brachmann (UC Irvine) for plasmid constructs.
Conceived and designed the experiments: MVW SFD. Performed the experiments: MVW MBS RAMdB. Analyzed the data: MVW MBS RAMdB HZ CW. Contributed reagents/materials/analysis tools: RAMdB HZ CW. Wrote the paper: MVW SFD.
- 1. Breeden L (1996) Start-specific transcription in yeast. Curr Top Microbiol Immunol 208: 95–127.L. Breeden1996Start-specific transcription in yeast.Curr Top Microbiol Immunol20895127
- 2. Pardee AB (1974) A restriction point for control of normal animal cell proliferation. Proc Natl Acad Sci U S A 71: 1286–1290.AB Pardee1974A restriction point for control of normal animal cell proliferation.Proc Natl Acad Sci U S A7112861290
- 3. Johnston GC, Pringle JR, Hartwell LH (1977) Coordination of growth with cell division in the yeast Saccharomyces cerevisiae. Exp Cell Res 105: 79–98.GC JohnstonJR PringleLH Hartwell1977Coordination of growth with cell division in the yeast Saccharomyces cerevisiae.Exp Cell Res1057998
- 4. Mendenhall MD, Hodge AE (1998) Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev 62: 1191–1243.MD MendenhallAE Hodge1998Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.Microbiol Mol Biol Rev6211911243
- 5. Spellman PT, Sherlock G, Zhang MQ, Iyer VR, Anders K, et al. (1998) Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization. Mol Biol Cell 9: 3273–3297.PT SpellmanG. SherlockMQ ZhangVR IyerK. Anders1998Comprehensive identification of cell cycle-regulated genes of the yeast Saccharomyces cerevisiae by microarray hybridization.Mol Biol Cell932733297
- 6. Cho RJ, Campbell MJ, Winzeler EA, Steinmetz L, Conway A, et al. (1998) A genome-wide transcriptional analysis of the mitotic cell cycle. Mol Cell 2: 65–73.RJ ChoMJ CampbellEA WinzelerL. SteinmetzA. Conway1998A genome-wide transcriptional analysis of the mitotic cell cycle.Mol Cell26573
- 7. Wittenberg C, Reed SI (2005) Cell cycle-dependent transcription in yeast: promoters, transcription factors, and transcriptomes. Oncogene 24: 2746–2755.C. WittenbergSI Reed2005Cell cycle-dependent transcription in yeast: promoters, transcription factors, and transcriptomes.Oncogene2427462755
- 8. Koch C, Moll T, Neuberg M, Ahorn H, Nasmyth K (1993) A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase. Science 261: 1551–1557.C. KochT. MollM. NeubergH. AhornK. Nasmyth1993A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase.Science26115511557
- 9. Costanzo M, Nishikawa JL, Tang X, Millman JS, Schub O, et al. (2004) CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast. Cell 117: 899–913.M. CostanzoJL NishikawaX. TangJS MillmanO. Schub2004CDK activity antagonizes Whi5, an inhibitor of G1/S transcription in yeast.Cell117899913
- 10. de Bruin RA, McDonald WH, Kalashnikova TI, Yates J 3rd, Wittenberg C (2004) Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5. Cell 117: 887–898.RA de BruinWH McDonaldTI KalashnikovaJ. Yates 3rdC. Wittenberg2004Cln3 activates G1-specific transcription via phosphorylation of the SBF bound repressor Whi5.Cell117887898
- 11. Dirick L, Bohm T, Nasmyth K (1995) Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae. Embo J 14: 4803–4813.L. DirickT. BohmK. Nasmyth1995Roles and regulation of Cln-Cdc28 kinases at the start of the cell cycle of Saccharomyces cerevisiae.Embo J1448034813
- 12. Tyers M, Tokiwa G, Futcher B (1993) Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins. Embo J 12: 1955–1968.M. TyersG. TokiwaB. Futcher1993Comparison of the Saccharomyces cerevisiae G1 cyclins: Cln3 may be an upstream activator of Cln1, Cln2 and other cyclins.Embo J1219551968
- 13. Cross FR (1988) DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae. Mol Cell Biol 8: 4675–4684.FR Cross1988DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.Mol Cell Biol846754684
- 14. Jorgensen P, Nishikawa JL, Breitkreutz BJ, Tyers M (2002) Systematic identification of pathways that couple cell growth and division in yeast. Science 297: 395–400.P. JorgensenJL NishikawaBJ BreitkreutzM. Tyers2002Systematic identification of pathways that couple cell growth and division in yeast.Science297395400
- 15. Wijnen H, Landman A, Futcher B (2002) The G(1) cyclin Cln3 promotes cell cycle entry via the transcription factor Swi6. Mol Cell Biol 22: 4402–4418.H. WijnenA. LandmanB. Futcher2002The G(1) cyclin Cln3 promotes cell cycle entry via the transcription factor Swi6.Mol Cell Biol2244024418
- 16. Sidorova JM, Mikesell GE, Breeden LL (1995) Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization. Mol Biol Cell 6: 1641–1658.JM SidorovaGE MikesellLL Breeden1995Cell cycle-regulated phosphorylation of Swi6 controls its nuclear localization.Mol Biol Cell616411658
- 17. Koch C, Schleiffer A, Ammerer G, Nasmyth K (1996) Switching transcription on and off during the yeast cell cycle: Cln/Cdc28 kinases activate bound transcription factor SBF (Swi4/Swi6) at start, whereas Clb/Cdc28 kinases displace it from the promoter in G2. Genes Dev 10: 129–141.C. KochA. SchleifferG. AmmererK. Nasmyth1996Switching transcription on and off during the yeast cell cycle: Cln/Cdc28 kinases activate bound transcription factor SBF (Swi4/Swi6) at start, whereas Clb/Cdc28 kinases displace it from the promoter in G2.Genes Dev10129141
- 18. Geymonat M, Spanos A, Wells GP, Smerdon SJ, Sedgwick SG (2004) Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6. Mol Cell Biol 24: 2277–2285.M. GeymonatA. SpanosGP WellsSJ SmerdonSG Sedgwick2004Clb6/Cdc28 and Cdc14 regulate phosphorylation status and cellular localization of Swi6.Mol Cell Biol2422772285
- 19. de Bruin RA, Kalashnikova TI, Chahwan C, McDonald WH, Wohlschlegel J, et al. (2006) Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback. Mol Cell 23: 483–496.RA de BruinTI KalashnikovaC. ChahwanWH McDonaldJ. Wohlschlegel2006Constraining G1-specific transcription to late G1 phase: the MBF-associated corepressor Nrm1 acts via negative feedback.Mol Cell23483496
- 20. Sun A, Bagella L, Tutton S, Romano G, Giordano A (2007) From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway. J Cell Biochem 102: 1400–1404.A. SunL. BagellaS. TuttonG. RomanoA. Giordano2007From G0 to S phase: a view of the roles played by the retinoblastoma (Rb) family members in the Rb-E2F pathway.J Cell Biochem10214001404
- 21. Harbour JW, Dean DC (2000) The Rb/E2F pathway: expanding roles and emerging paradigms. Genes Dev 14: 2393–2409.JW HarbourDC Dean2000The Rb/E2F pathway: expanding roles and emerging paradigms.Genes Dev1423932409
- 22. Kaelin WG Jr (1999) Functions of the retinoblastoma protein. Bioessays 21: 950–958.WG Kaelin Jr1999Functions of the retinoblastoma protein.Bioessays21950958
- 23. Ho A, Dowdy SF (2002) Regulation of G(1) cell-cycle progression by oncogenes and tumor suppressor genes. Curr Opin Genet Dev 12: 47–52.A. HoSF Dowdy2002Regulation of G(1) cell-cycle progression by oncogenes and tumor suppressor genes.Curr Opin Genet Dev124752
- 24. Ezhevsky SA, Nagahara H, Vocero-Akbani AM, Gius DR, Wei MC, et al. (1997) Hypo-phosphorylation of the retinoblastoma protein (pRb) by cyclin D:Cdk4/6 complexes results in active pRb. Proc Natl Acad Sci U S A 94: 10699–10704.SA EzhevskyH. NagaharaAM Vocero-AkbaniDR GiusMC Wei1997Hypo-phosphorylation of the retinoblastoma protein (pRb) by cyclin D:Cdk4/6 complexes results in active pRb.Proc Natl Acad Sci U S A941069910704
- 25. Knudsen ES, Wang JY (1996) Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites. J Biol Chem 271: 8313–8320.ES KnudsenJY Wang1996Differential regulation of retinoblastoma protein function by specific Cdk phosphorylation sites.J Biol Chem27183138320
- 26. Kitagawa M, Higashi H, Jung HK, Suzuki-Takahashi I, Ikeda M, et al. (1996) The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2. Embo J 15: 7060–7069.M. KitagawaH. HigashiHK JungI. Suzuki-TakahashiM. Ikeda1996The consensus motif for phosphorylation by cyclin D1-Cdk4 is different from that for phosphorylation by cyclin A/E-Cdk2.Embo J1570607069
- 27. Brown VD, Phillips RA, Gallie BL (1999) Cumulative effect of phosphorylation of pRB on regulation of E2F activity. Mol Cell Biol 19: 3246–3256.VD BrownRA PhillipsBL Gallie1999Cumulative effect of phosphorylation of pRB on regulation of E2F activity.Mol Cell Biol1932463256
- 28. Ezhevsky SA, Ho A, Becker-Hapak M, Davis PK, Dowdy SF (2001) Differential regulation of retinoblastoma tumor suppressor protein by G(1) cyclin-dependent kinase complexes in vivo. Mol Cell Biol 21: 4773–4784.SA EzhevskyA. HoM. Becker-HapakPK DavisSF Dowdy2001Differential regulation of retinoblastoma tumor suppressor protein by G(1) cyclin-dependent kinase complexes in vivo.Mol Cell Biol2147734784
- 29. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, et al. (1998) Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953–961.MS LongtineA. McKenzie 3rdDJ DemariniNG ShahA. Wach1998Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae.Yeast14953961
- 30. Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, et al. (1998) Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115–132.CB BrachmannA. DaviesGJ CostE. CaputoJ. Li1998Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications.Yeast14115132
- 31. Smolka MB, Albuquerque CP, Chen SH, Schmidt KH, Wei XX, et al. (2005) Dynamic changes in protein-protein interaction and protein phosphorylation probed with amine-reactive isotope tag. Mol Cell Proteomics 4: 1358–1369.MB SmolkaCP AlbuquerqueSH ChenKH SchmidtXX Wei2005Dynamic changes in protein-protein interaction and protein phosphorylation probed with amine-reactive isotope tag.Mol Cell Proteomics413581369
- 32. Flick KM, Spielewoy N, Kalashnikova TI, Guaderrama M, Zhu Q, et al. (2003) Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters. Mol Biol Cell 14: 3230–3241.KM FlickN. SpielewoyTI KalashnikovaM. GuaderramaQ. Zhu2003Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters.Mol Biol Cell1432303241
- 33. Jeoung DI, Oehlen LJ, Cross FR (1998) Cln3-associated kinase activity in Saccharomyces cerevisiae is regulated by the mating factor pathway. Mol Cell Biol 18: 433–441.DI JeoungLJ OehlenFR Cross1998Cln3-associated kinase activity in Saccharomyces cerevisiae is regulated by the mating factor pathway.Mol Cell Biol18433441
- 34. Tyers M, Futcher B (1993) Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes. Mol Cell Biol 13: 5659–5669.M. TyersB. Futcher1993Far1 and Fus3 link the mating pheromone signal transduction pathway to three G1-phase Cdc28 kinase complexes.Mol Cell Biol1356595669
- 35. Queralt E, Igual JC (2003) Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling. Mol Cell Biol 23: 3126–3140.E. QueraltJC Igual2003Cell cycle activation of the Swi6p transcription factor is linked to nucleocytoplasmic shuttling.Mol Cell Biol2331263140
- 36. Boustany LM, Cyert MS (2002) Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site. Genes Dev 16: 608–619.LM BoustanyMS Cyert2002Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site.Genes Dev16608619
- 37. DeVit MJ, Johnston M (1999) The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae. Curr Biol 9: 1231–1241.MJ DeVitM. Johnston1999The nuclear exportin Msn5 is required for nuclear export of the Mig1 glucose repressor of Saccharomyces cerevisiae.Curr Biol912311241
- 38. Kaffman A, Rank NM, O'Neill EM, Huang LS, O'Shea EK (1998) The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus. Nature 396: 482–486.A. KaffmanNM RankEM O'NeillLS HuangEK O'Shea1998The receptor Msn5 exports the phosphorylated transcription factor Pho4 out of the nucleus.Nature396482486
- 39. Ptacek J, Devgan G, Michaud G, Zhu H, Zhu X, et al. (2005) Global analysis of protein phosphorylation in yeast. Nature 438: 679–684.J. PtacekG. DevganG. MichaudH. ZhuX. Zhu2005Global analysis of protein phosphorylation in yeast.Nature438679684
- 40. Glover CV 3rd (1998) On the physiological role of casein kinase II in Saccharomyces cerevisiae. Prog Nucleic Acid Res Mol Biol 59: 95–133.CV Glover 3rd1998On the physiological role of casein kinase II in Saccharomyces cerevisiae.Prog Nucleic Acid Res Mol Biol5995133
- 41. Nash P, Tang X, Orlicky S, Chen Q, Gertler FB, et al. (2001) Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication. Nature 414: 514–521.P. NashX. TangS. OrlickyQ. ChenFB Gertler2001Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication.Nature414514521
- 42. Di Como CJ, Chang H, Arndt KT (1995) Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2. Mol Cell Biol 15: 1835–1846.CJ Di ComoH. ChangKT Arndt1995Activation of CLN1 and CLN2 G1 cyclin gene expression by BCK2.Mol Cell Biol1518351846
- 43. Wijnen H, Futcher B (1999) Genetic analysis of the shared role of CLN3 and BCK2 at the G(1)-S transition in Saccharomyces cerevisiae. Genetics 153: 1131–1143.H. WijnenB. Futcher1999Genetic analysis of the shared role of CLN3 and BCK2 at the G(1)-S transition in Saccharomyces cerevisiae.Genetics15311311143
- 44. Lees JA, Buchkovich KJ, Marshak DR, Anderson CW, Harlow E (1991) The retinoblastoma protein is phosphorylated on multiple sites by human cdc2. Embo J 10: 4279–4290.JA LeesKJ BuchkovichDR MarshakCW AndersonE. Harlow1991The retinoblastoma protein is phosphorylated on multiple sites by human cdc2.Embo J1042794290
- 45. Mittnacht S, Lees JA, Desai D, Harlow E, Morgan DO, et al. (1994) Distinct sub-populations of the retinoblastoma protein show a distinct pattern of phosphorylation. Embo J 13: 118–127.S. MittnachtJA LeesD. DesaiE. HarlowDO Morgan1994Distinct sub-populations of the retinoblastoma protein show a distinct pattern of phosphorylation.Embo J13118127