The ubiquitous bacterial trans-translation is one of the most studied quality control mechanisms. Trans-translation requires two specific factors, a small RNA SsrA (tmRNA) and a protein co-factor SmpB, to promote the release of ribosomes stalled on defective mRNAs and to add a specific tag sequence to aberrant polypeptides to direct them to degradation pathways. Helicobacter pylori is a pathogen persistently colonizing a hostile niche, the stomach of humans.
We investigated the role of trans-translation in this bacterium well fitted to resist stressful conditions and found that both smpB and ssrA were essential genes. Five mutant versions of ssrA were generated in H. pylori in order to investigate the function of trans-translation in this organism. Mutation of the resume codon that allows the switch of template of the ribosome required for its release was essential in vivo, however a mutant in which this codon was followed by stop codons interrupting the tag sequence was viable. Therefore one round of translation is sufficient to promote the rescue of stalled ribosomes. A mutant expressing a truncated SsrA tag was viable in H. pylori, but affected in competence and tolerance to both oxidative and antibiotic stresses. This demonstrates that control of protein degradation through trans-translation is by itself central in the management of stress conditions and of competence and supports a regulatory role of trans-translation-dependent protein tagging. In addition, the expression of smpB and ssrA was found to be induced upon acid exposure of H. pylori.
We conclude to a central role of trans-translation in H. pylori both for ribosome rescue possibly due to more severe stalling and for protein degradation to recover from stress conditions frequently encountered in the gastric environment. Finally, the essential trans-translation machinery of H. pylori is an excellent specific target for the development of novel antibiotics.
Citation: Thibonnier M, Thiberge J-M, De Reuse H (2008) Trans-Translation in Helicobacter pylori: Essentiality of Ribosome Rescue and Requirement of Protein Tagging for Stress Resistance and Competence. PLoS ONE3(11): e3810. https://doi.org/10.1371/journal.pone.0003810
Editor: Niyaz Ahmed, Centre for DNA Fingerprinting and Diagnostics, India
Received: September 1, 2008; Accepted: October 29, 2008; Published: November 26, 2008
Copyright: © 2008 Thibonnier et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: MT was supported by a FRM grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Helicobacter pylori is a gram negative bacterial pathogen that infects the stomach of about half of the world population. H. pylori is mostly acquired during childhood and the infection persists during decades unless patients receive an eradication treatment. Persistent colonization is concomitant with a strong inflammation of the mucosal layer triggering gastric pathologies such as gastritis, duodenal and peptic ulcer, adenocarcinoma or MALT lymphoma . Lifelong colonization of the gastric mucosa by H. pylori implies that this bacterium is well adapted to this hostile environment facing both permanent acid stress in the mucus layer and oxidative stress at the gastric epithelium due to the host's immune response . The mechanisms involved in the recovery from damages caused by the exposure to stress are critical in the adaptive response. These involve both active repair procedures (well studied for oxidative stress in H. pylori ) and quality control mechanisms.
In the present study, we addressed the role of trans-translation in H. pylori. Trans-translation is one of the most studied quality control mechanisms that provides bacteria with a general surveillance of the flow of genetic information , , , . This mechanism rescues ribosomes sequestered on defective mRNAs lacking appropriate termination signals hence unable to efficiently resume the translation process. In addition, trans-translation promotes decay of these defective mRNAs and adds an amino acid tag to the truncated proteins to direct them to degradation pathways. Trans-translation relies on the properties of SsrA, a small stable RNA also called tmRNA, which shares features with a tRNA and a mRNA , . Studies mainly performed on the E. coli system established the following mechanism of trans-translation. First, alanylated SsrA forms a complex with essential protein partners SmpB and EF-Tu and acts as a tRNA by allowing the nascent polypeptide encoded by the defective mRNA to be transferred onto the tRNAAla-like domain of SsrA. Then, a short coding sequence within SsrA, referred to as the tag sequence behaves like a surrogate mRNA. This tag sequence provides the stalled ribosome with a new template for translation that is terminated by an in-frame stop codon; thus, allowing the release of recyclable ribosomal subunits, and the addition of a C-terminal tag to the nascent peptide. These tagged trans-translated polypeptides are specifically targeted to mainly ATP-dependent proteases ,  and the defective mRNAs are degraded by RNase R .
While the overall mechanistic of trans-translation and the origin of defective or broken mRNAs have been extensively studied, questions on the precise biological role of this system are only partially answered. It was shown that normally growing cells undergo frequent trans-translation events . In addition, there appears to be some specificity in the proteins tagged by tmRNA under normal growth conditions. . Situations favoring stalling of ribosomes which are shown to require trans-translation are typically use of miscoding antibiotics , premature transcription termination or ribonucleolytic cleavage by RNases. Although stress or starvation are thought to enhance the amount of defective mRNAs, little is known about the actual damages occurring to ribonucleic acids under these conditions. The general assumption is that these damages are similar to those of DNA molecule i.e. generation of base adducts upon alkylation  or single stranded-breaks due to ROS (Reactive Oxygen Species) .
Genes encoding SsrA and its protein co-factor SmpB are conserved among bacteria  and are generally dispensable. Surprisingly, despite this conservation no common physiological function of trans-translation was found in the different bacterial systems studied. Mutants defective in trans-translation exhibit a wide range of phenotypes related to regulation of cellular physiology, cell cycle timing, stress response or virulence , . The precise reason why trans-translation is associated with these functions is rarely understood. In E. coli, inactivation of ssrA leads to reduced growth rate, delayed recovery from carbon starvation and temperature sensitivity , . In Bacillus subtilis, tmRNA dependent growth was shown during temperature or chemical stress conditions that correlated with an increase of the cellular amounts of SsrA . E. coli, Salmonella or Synechocystis strains defective in trans-translation were found to be hypersensitive to different antibiotics , . Deficiency in trans-translation affects the ability of Salmonella enterica serovar Typhimurium to colonize mice  and to survive within macrophages . A ΔssrA-smpB mutant of Yersinia pseudotuberculosis is avirulent in a mouse infection model, this is due to a loss in the induction of known virulence factors (motility, Type 3 secretion system) . tmRNA also has a regulatory role for the correct timing of cell cycle regulation of C. crescentus . Interestingly, SsrA with a protease-resistant SsrA tag does not restore motility or proper DNA replication in Y. pseudotuberculosis and C. crescentus, respectively , . However, the role of the tag in stress response was never investigated.
The essentiality of the smpB gene has been deduced from systematic gene interruption studies performed in only three organisms Mycoplasma genitalium , Mycoplasma pulmonis  and Haemophilus influenzae . Essentiality of ssrA was only demonstrated in Neisseria gonorrhoeae . Due to the difficulties in studying essential functions inside the cells, little is known about trans-translation in species in which it is required for in vitro growth. Only in N. gonorrhoeae was this phenotype analyzed further. It was demonstrated that the essential function of trans-translation is the ribosome rescue whereas tagging activity was dispensable .
We decided to investigate the role of trans-translation in H. pylori because this bacterium is permanently subjected to stressful conditions that could increase the occurrence of premature transcription termination events. While the predicted H. pylori tmRNA structure and essential residues were conserved in comparison with those of the well-studied molecule of E. coli, the tag sequence of H. pylori presented some striking differences. This manuscript presents the demonstration of the essential character of trans-translation during in vitro growth of H. pylori and the investigation of its functional characteristics by site directed mutagenesis. We showed that residues necessary for ribosome rescue by SsrA are essential for H. pylori growth and that the tagging of trans-translated proteins is required for its adaptation to stressful conditions and for competence.
smpB and ssrA are essential genes in H. pylori
Attempts to inactivate the smpB and ssrA genes encoded by hp1444 and hp0784, respectively, in several H. pylori backgrounds were repeatedly unsuccessful suggesting that these genes and the trans-translation process are essential for in vitro growth of H. pylori. In parallel, gene hp1248 predicted to encode RnaseR was deleted showing that this function is dispensable in H. pylori. To formally demonstrate the essentiality of ssrA and smpB, H. pylori strain N6 was first transformed by stably replicating plasmids pILL788 and pILL786 expressing ssrA or smpB, respectively, under control of an IPTG inducible promoter derived from pILL2150  (Tables 1 and S1). Deletions of the ssrA chromosomal copy of strain N6 pILL788 and the smpB chromosomal copy of strain N6 pILL786 were obtained after transformation by suicide plasmids in the presence of IPTG as illustrated in Figure 1. These suicide plasmids carried a kanamycin resistance cassette flanked by DNA regions situated immediately upstream and downstream of the genes to be inactivated, thereby forcing homologous recombination outside the coding sequences of ssrA or smpB and thus specifically targeting allelic exchange into the chromosomal gene copy. When N6 carrying the empty vector pILL2150 was transformed with either of the two suicide plasmids, we obtained either no kanamycin resistant clones or a couple of clones that were either non viable or had undergone illegitimate recombination (Fig. 1). This demonstrated that ssrA and smpB are essential genes in H. pylori and prompted the investigation of their roles in this pathogen.
A) pILL786 carrying wild-type smpB, or B) pILL788 carrying wild-type ssrA or different plasmids with mutagenized versions of ssrA (short names of the mutations are indicated) by suicide plasmids designed to create chromosomal deletions of smpB or ssrA, respectively. A strain carrying the empty vector pILL2150 served as a negative control. The transformation frequency is calculated as the number of transformants obtained for 5×108 cells and 1 µg of DNA and expressed as a percentage of that by plasmids with wild-type smpB or ssrA.
SmpB depletion in H. pylori results in growth arrest
Construction of strain N6ΔsmpB pILL786 and strain N6ΔssrA pILL788 provided us with valuable H. pylori smpB or ssrA conditional mutants. The impacts of SmpB or SsrA depletion on H. pylori growth was measured. Notably, SsrA stability has been shown to be diminished in the absence of SmpB . After approx. two doubling times in liquid medium without inducer, the conditional smpB mutant stopped dividing as a consequence of SmpB depletion (Fig. 2). Interestingly, we observed that SmpB depletion stops bacterial division but does not cause cell death growth. Indeed, the SmpB conditional mutant could be rescued if plated in the presence of the inducer IPTG (until 24 h growth, data not shown). In contrast, after 24 h, growth rescue was not possible suggesting that SmpB-depleted bacteria had undergone a physiological switch irreversibly directing them towards bacterial death.
The following strains N6 pILL2150 (empty vector), N6ΔsmpB pILL786 (vector expressing the SmpB protein under control of an inducible promoter Ptac) were grown with the inducer IPTG 1 mM (+I) or without inducer (-I). An arrow indicates the arrest of growth of strain N6ΔsmpB pILL786. The standard deviations for 5 different measurements are shown by error bars.
Similar experiments with the SsrA conditional mutant did not result in observable bacterial growth arrest. We concluded that the number of bacterial divisions occurring between the inoculation and the entry of the cells into stationary growth phase was not sufficient to dilute the intracellular concentration of SsrA to a level critical for cell growth. Indeed, northern blots revealed significant SsrA over-expression in H. pylori strain N6ΔssrA pILL788 (Fig. 3A). In agreement with this interpretation is the documented high stability of SsrA molecules .
Panel A: H. pylori expressing wild type SsrA, different mutant versions of SsrA or over-expressing wild type SsrA from plasmid pILL788. Panel B: H. pylori wild type strain incubated at different pH values. Normalization was performed with 5S rRNA probes. The ladder corresponds to DNA of pBR322 plasmid digested by MspI, labeled and denaturated.
Upon IPTG induction, both SmpB and SsrA conditional mutants presented a slightly higher growth rate when compared with the wild type strain (for SmpB, see Fig. 2). This suggested that enhancing the trans-translation process improves the fitness of H. pylori under these conditions.
Site directed mutagenesis of H. pylori ssrA
To investigate the different functions of SsrA in the trans-translation process, five different mutations were introduced in the ssrA gene on plasmid pILL788. Figure 4 illustrates a model of the H. pylori SsrA (tmRNA) molecule based on the predictions of the tmRNA web site (http://www.indiana.edu/̃tmrna/). The residues for which a defined function in ribosome rescue was assigned in the well-studied E. coli tmRNA presented strong conservation (Fig. 4). Interestingly, the tag sequence from H. pylori showed several differences when compared with that of the previously studied tmRNAs from E. coli, N. gonorrhoeae or C. crescentus. The positions of the mutations analyzed in the present study have been emphasized in Fig. 4. The first two mutations targeted residues that were identified to be required for the interaction of SsrA with factors involved in the trans-translation process in E. coli. First, the predicted SmpB interaction site of SsrA  was modified by the introduction of three consecutive mutations G19U-A20U-C21A, and this mutant was designated SsrASmpB. Second, the G•U mismatch in the tRNAAla-like domain of SsrA was targeted. Recognition of this mismatch by the alanyl-tRNA synthetase is mandatory for the addition of Ala at the 3′ end of SsrA . This mutant designated SsrAwobble carried a U380C modification. Next, by substituting the resume codon GUA (positions 84-85-86) by a stop codon (UAA) the restart of translation was abolished. This type of mutant designated SsrAresume was not tested before in vivo for essentiality in another organism. To specifically study the role of the tmRNA-dependent protein tagging in H. pylori, two different mutations in the tag region of ssrA were introduced. These mutations would uncouple the two functions of tmRNA, ribosome rescue and protein tagging for degradation. In one mutant, the two terminal codons of the tag region coding for Alanine were changed into Aspartate codons (SsrADD in Fig. 4). In E. coli, non polar residues in the C-terminus part of the tag (ALAA) are critical for recognition by cellular proteases ,  and their mutation causes stabilization of the trans-translated peptides. We also wanted to examine the behavior of the H. pylori essential tmRNA under conditions in which a minimal tag was appended to the truncated peptides generated by trans-translation events. Therefore, the second and the third codons of the tag sequence were replaced by two stop codons (UAA-UGA), the mutant was designated SsrASTOP.
The positions of the mutations studied in this work are emphasized in the figure. Designation of the mutations and targeted functions: SsrASmpB: mutation in the interaction site between SsrA and SmpB, G19U; A20U; C21A. SsrAwobble: mutation of the wobble G•U in the tRNAAla-like domain, U380C. SsrAresume: substitution of the resume codon by a stop codon, G84U; U85A ; A86A. SsrADD: substitution of the two terminal alanine codons of the tag by asparate codons. SsrASTOP: introduction of two stop codons downstream from the resume codon; A87U; C89A; A90U; C91G; C92A. A table is presented below that summarizes the encoded tag sequences of some bacteria and that indicates the product of the mutated SsrA tag sequences. Filled circles represent stop codons.
Identification of essential residues in the H. pylori tmRNA
Plasmids carrying mutated ssrA were introduced into H. pylori strain N6 (Table 1). To evaluate the impact of these mutations on H. pylori viability, these strains were transformed with the suicide plasmid pILL796 designed to delete the chromosomal copy of ssrA and the number of transformants on selective medium were counted (Fig. 1). The frequency of transformation was determined by calculating the number of transformants for a given amount of viable cells (5×108 bacteria) with 1 µg DNA of the suicide plasmid (pILL796). The transformation frequency of the chromosomal deletion of ssrA in a recipient strain N6 carrying the wild type ssrA plasmid (pILL788) was estimated to be 2×10−3. Identical transformation frequencies of strains carrying pILL791 with SsrADD and pILL2328 with SsrASTOP were obtained that were similar to that of N6 with SsrAwt (Fig. 1 B). The frequency of transformation was at least four orders of magnitude lower for the inactivation attempts of strains carrying one of the three mutations affecting the ribosome rescue process, SsrAresume (pILL792), SsrAwobble (pILL793) and SsrAsmpB (pILL794) (Fig. 1 B). This data showed that each of these essential steps of the trans-translation process is essential in H. pylori. In contrast, the mutations affecting the tag do not impact bacterial viability. Importantly, viability of the SsrASTOP mutant appending a minimal tag (Ala from tmRNA and Val from the resume codon) suggests that one round of translation is sufficient to rescue the stalled ribosomes. This latter mutant allowed us to evaluate the role of protein tagging in vivo under conditions that were more drastic than the point mutations affecting tag recognition described in previous studies.
Mutations in the tag of the tmRNA are viable in H. pylori and do not affect in vivo colonization
To analyze the phenotype of H. pylori mutants with a modified tmRNA tag, SsrADD and SsrASTOP mutations were introduced by allelic exchange into the chromosome replacing the wild type ssrA alleles in three different H. pylori backgrounds N6, X47-2AL and 26695 (Table S1). N6 is a strain in which the shuttle plasmid replicates in a stable manner, X47-2AL is a mouse-adapted strain and 26695 is a strain from which the entire genome has been sequenced. The expression level and stability of the mutated versions of SsrA in H. pylori strain 26695 were identical to that of the wild type SsrA (Fig. 3A).
Mutants were obtained in every strain as expected (Table S1) and their growth under normal conditions was not affected. These strains were used to evaluate the role of tagging under several conditions relevant to the gastric niche of H. pylori such as growth at pH 5.5 (mutants of strain 26695), motility and colonization of a mouse model (mutants of strain X47-2AL) (data not shown). The mutants behaved like the corresponding isogenic wild type strains under the conditions tested. It was concluded that the tagging process of trans-translation is not essential for in vivo survival and motility of H. pylori.
Assessment of SsrA mediated protein tagging in H. pylori strains expressing mutant SsrA versions
To examine the actual protein tagging activities in H. pylori, we engineered a pair of artificial trans-translation target proteins (Fig. 5 A) composed of a fusion between the non-essential gene hypB (coding for H. pylori hydrogenase accessory protein) and a sequence encoding protein A from Staphylococcus aureus that could easily be detected by western blotting. This gene fusion designated hypB-TAP is described in Stingl et al. . Our aim was to evaluate the fate of these target proteins when expressed in H. pylori mutants defective in tagging activity. Therefore, two constructions were generated, one was terminated by a translational stop codon and the other devoid of a stop codon, both were followed by a transcriptional terminator. Western blots in E. coli (Fig. 5 B) indicated that these constructs behaved like efficient trans-translation tagging target proteins. In E. coli MG1655 wild-type strain, the protein fusion with stop (expressed by pILL2332) was expressed in large amounts while that without stop (pILL2333) was less present indicating that protein degradation had occurred (Fig. 5 B). Involvement of trans-translation in the degradation of HypB-TAP fusion without stop was demonstrated by the strong stabilization of this protein in an E. coli ΔssrA strain (Fig. 5 B) in contrast to the amounts of the fusion with stop that were unchanged. Given the large Molec Mass (50 kDa) of the HypB-TAP fusion, addition of the 1.5 kDa tag by trans-translation was not visible on an acrylamide gel.
A) Construction strategy of the reporter genes and predicted fate of the encoded proteins. B) Western-blots of whole cell extracts from E. coli or H. pylori strains expressing HypB-TAP with and without the terminal stop codon (MM 47 kDa) and revealed by peroxidase coupled anti-peroxidase antibody that binds to the protein A motif. C) Loading controls are presented: line 1 and 5 correspond to crude extracts of SsrA wild type E. coli and H. pylori strains expressing HypB-TAP with STOP respectively, line 2 and 6 correspond to whole extracts of ΔssrA E. coli and SsrADD H. pylori mutants expressing HypB-TAP with STOP respectively, line 3 and 7 correspond to the whole extracts of SsrA wild type E. coli and H. pylori strains expressing HypB-TAP without STOP respectively and line 4 and 8 correspond to whole extracts of ΔssrA WT E. coli and SsrADD H. pylori mutants expressing HypB-TAP without STOP respectively.
We then decided to introduce by natural transformation the two reporter genes hypB-TAP with or without stop either expressed from plasmids (pILL2332 and pILL2333) or directly by recombination on the chromosome of H. pylori N6 wild type strain and of each of the two tag mutants. The wild type strain was transformed by the constructs at expected frequencies. In contrast, transformation efficacy was repeatedly diminished in both SsrA mutants; SsrADD strain presented a three fold lower efficacy and no transformants were obtained in the SsrASTOP background (three independent experiments). Similar observations were made when a suicide plasmid targeting allelic exchange into ureA-B (described in ) was used as a control. The loss of competence of the SsrASTOP mutant was unexpected and suggested that trans-translation dependent tagging is required for natural transformation in H. pylori.
As in E. coli, we found that in H. pylori wild type strain, HypB-TAP without stop expressed from the chromosome was heavily degraded (Fig. 5B) as compared to HypB-TAP with stop suggesting that it was indeed targeted by trans-translation. The SsrADD mutant only marginally stabilized the HypB-TAP without stop protein (about two fold) indicating that it was still subject to proteolysis.
Minimal trans-translation-dependent protein tagging leads to increased sensitivity of H. pylori to antibiotics and oxidative stress
The role of the trans-translation dependent protein tagging in H. pylori strain 26695 after exposure to two types of stresses was addressed (Fig. 6). First, susceptibility to sub-lethal doses of two antibiotics was examined (i) chloramphenicol, a peptidyl transferase inhibitor that targets the translation machinery and, (ii) amoxicillin that irreversibly binds to the active site of penicillin-binding proteins (PBPs) involved in cell wall biosynthesis. Amoxicillin is one of the recommended components of the triple therapy employed in anti-H. pylori treatment. Second, the response of the mutants to oxidative stress was tested by measuring the sensitivity to paraquat (methyl viologen) that generates superoxide radicals. Superoxide radicals are among the molecules synthesized during the oxidative burst of immune cells. The SsrADD mutant behaved like the wild type strain during exposure to chloramphenicol, amoxicillin and paraquat (Fig. 6). In striking contrast, SsrASTOP presented an enhanced sensitivity to chloramphenicol stress for doses of 2.0 and 2.5 µg.ml−1 and to amoxicillin with lethality at 0.6 µg.ml−1 (Fig. 6). In addition, the SsrASTOP mutant that has a minimal tag sequence presented higher sensitivity to oxidative stress upon exposure to paraquat (Fig. 6).
Acid stress causes induction of both ssrA and smpB
The association of trans-translation with the response to stress and the continual exposure of H. pylori to the acidity of its gastric niche lead us to ask whether this mechanism could provide the cell with a rapid adaptive response to stressful conditions. In a previous transcriptomic study, we detected smpB gene induction upon acid exposure of H. pylori strain 26695 . Acid activation of smpB was validated with RT-PCR  and more recently by Northern blotting analysis (data not shown). ssrA messenger RNA was examined by Northern blots on total RNA extracted from exponential growing H. pylori cells (strain 26695) incubated for 30 min at pH 7, pH 4.5 or pH 2. We observed a band that corresponded to a molecule of 386 nt which is the length expected for a mature SsrA (as predicted from the 26695 genome sequence) (Fig. 3B). For bacteria exposed to pH 2 and pH 4.5, this band was significantly more intense than for bacteria exposed to pH 7 (Fig. 3B). This suggests that in H. pylori, SsrA amounts are increased at low pH.
While the mechanistic and structural aspects of trans-translation and of tmRNA have been extensively studied, several questions remain concerning the biological role of this system. It was established that under normal growth conditions, a specific pattern of proteins are targeted by tmRNA . Yet, the function of this process in the cell is not clear. The role of trans-translation in ribosome rescue under stress conditions has been demonstrated, although the importance of tagging truncated proteins was not known. In addition, the essentiality of trans-translation in some bacterial species is not understood. The two latter issues and the role of this quality control mechanism in the pathogen H. pylori was addressed due to its exceptional ability to persist in a harsh environment.
Essentiality of trans-translation in H. pylori and in other organisms
Both ssrA and smpB were demonstrated to be essential in H. pylori. Using a conditional expression system, SmpB depletion in H. pylori cells resulted in growth arrest that was not associated with immediate cell death, that only occurred after 24 h depletion. This suggested that no irreversible process or toxic product accumulation occurred when trans-translation was inactivated. The reason why trans-translation is essential in some organisms is still not understood but several hypothesis were raised. Essentiality of trans-translation has been proposed to be associated with small genomes or with the necessity to accurately manage a restricted pool of ribosomes expressed by a limited number of rRNA operons . Table 2 summarizes the available data on trans-translation essentiality or dispensability in several bacteria with their genome size, the number of rRNA operons and the duplication time. It can be concluded that there is no correlation between any of these criteria and trans-translation essentiality. In particular, the proposed correlation between trans-translation essentiality and a reduced number of rRNA operons  has not been confirmed by this analysis. Slow growth rates that are associated with a reduced number of rRNA can also be excluded as a cause of trans-translation essentiality (Table 2). While essentiality was originally thought to be associated with small genomes, this notion is contradicted by the recent example of Shigella flexneri  rendering unlikely the hypothesis of the absence of an alternative mechanism for mRNA quality control in bacteria with reduced coding capacity. Other interpretations of trans-translation essentiality during normal growth conditions can be proposed. The accumulation of truncated proteins or mRNAs may be lethal or tmRNA-dependent tagging of a specific protein could be essential for bacterial survival. This was shown not to be the case in H. pylori since (i) tagging is not the essential function of trans-translation in H. pylori and, (ii) RnaseR, a conserved ribonuclease likely to be responsible for the degradation of defective messengers is dispensable.
Interestingly, we observed that over-expression of either SsrA or SmpB enhances the in vitro growth rate of H. pylori suggesting an increase in the fitness of the bacterium under these normal conditions. In B. subtilis, while trans-translation is not essential under normal growth conditions, cells grew depending on the expression level of SsrA under stress conditions such as high temperature . Therefore, it can be proposed that (i) H. pylori cells grown in vitro are submitted to some type of stress that produces damaged RNAs at a high occurrence and/or causes frequent ribosome pausing and, (ii) that in this bacterium, trans-translation components represent a limiting factor for normal growth. This could be related to the fact that H. pylori has intrinsically an elevated mutation rate compared to most other bacteria .
Ribosome rescue with an intact resume codon is an essential function of trans-translation in H. pylori
The essentiality of several point mutations in ssrA was tested in H. pylori. Mutations in the SsrA tag sequence of H. pylori were viable. The lethality of SsrA mutations affecting the tRNAAla-like domain (wobble), the interaction with SmpB and the resume codon for the restart of translation after ribosome stalling indicated that, in H. pylori, rescue of stalled ribosomes by trans-translation is essential. The two latter mutations were particularly interesting, since they were never tested in vivo for essentiality. In vitro studies showed that resume of the translation is mandatory for the dissociation of the stalled ribosome . However, here we show that a single ribosomal translocation step is sufficient to allow its recycling since the mutant carrying stop codons instead of the second and third codons of the tag (SsrASTOP) is viable. In N. gonorrhoeae, the essential function of trans-translation was also associated with ribosome rescue and not with protein tagging .
Viability of H. pylori mutants with a minimal tmRNA tag sequence
While mutation of the resume codon was lethal in H. pylori, introduction of two stop codons immediately after this position that restricted the added tag to only two amino acids did not affect H. pylori growth under normal conditions. This provided us with a valuable tool to examine in vivo the role of tagging of truncated peptides generated under conditions of functional trans-translation. Mutations in the tag sequence are expected to stabilize these peptides by preventing their recognition by specific proteases well defined in E. coli  and C. crescentus  and conserved in H. pylori . The two last Ala codons of the tag (Fig. 4) have been reported to be critical for this recognition in several organisms. H. pylori SsrADD strain carrying such a mutation only weakly stabilized the artificial trans-translation target protein (HypB-TAP). We concluded that in contrast to what was described in E. coli or B. subtilis, these two conserved codons of the tag are not central for protease recognition in H. pylori. In addition, the H. pylori tag sequence presents two striking differences with those of E. coli, B. subtilis, N. gonorrhoeae and C. crescentus that could reflect differences in the degradation process. This includes the presence of a polar residue at the ante penultimate position in the last four amino acids of the proteolysis tag (AKAA in H. pylori instead of AL/VAA, Fig. 4) and the absence of a SspB recognition motif, a proteolytic adaptor predicted to be absent in H. pylori.
Role of the trans-translation dependent tagging under stress conditions and for efficient DNA transformation
An original outcome of this study came from our observation that under conditions of functional ribosome rescue, the tagging of trans-translated protein was necessary for stress resistance and competence. Till now, in other organisms only mutants carrying deletions of the entire tmRNA or of smpB (deficient in both trans-translation functions) were examined for stress sensitivity.
The H. pylori SsrASTOP mutant presented a multifaceted phenotype including (i) increased susceptibility to sub-lethal doses of chloramphenicol, (ii) hypersensitivity to amoxicillin, and (iii) deficient natural transformation capacity. In agreement with our previous conclusions, these phenotypes were not or only very marginally displayed by the SsrADD mutant.
In E. coli, sub-lethal concentrations of miscoding antibiotics such as kanamycin are known to enhance SsrA protein tagging activity due to translational read-through at normal stop codons, however read-through rarely occurs with chloramphenicol . Alternatively, translation velocity reduction by chloramphenicol might increase the amount of cleaved mRNAs and thus the recruitment of tmRNA . Bactericidal antibiotics such as amoxicillin targeting the cell wall synthesis are obviously not directly interfering with translation. Increased sensitivity to ampicillin for an E. coli ΔssrA mutant has been reported  while mutants of Synechocystis or Y. pseudotuberculosis did not display this phenotype , . This class of antibiotics have recently been shown to stimulate production of hydroxyl radicals that damage nucleic acids including mRNA and therefore might indirectly require trans-translation . The continual oxidative stress encountered by H. pylori at its colonization site represents a major challenge despite H. pylori being well-equipped to protect itself from ROS . The SsrASTOP mutant exhibits a striking hypersensitivity to ROS. Importantly, these results demonstrate for the first time that the tagging process is by itself important for the response to stress conditions. Stress could enhance the amount of truncated mRNAs either directly or through ribosome pausing and, as a consequence produce toxic accumulation of truncated untagged peptides. Alternatively, recovery from stress conditions might require trans-translation of specific proteins.
We found a novel role of trans-translation in natural transformation competence that, in H. pylori, depends on the comB Type IV secretion system . Our results point to the need of trans-translation tagging of a specific protein required for efficient activity of this system. Noteworthy, trans-translation deletion mutants of Y. pseudotuberculosis are deficient in the delivery of Yop proteins by a Type III secretion system . The existence of a common trans-translation dependent check-point mechanism required for the assembly of these two secretion systems is an attractive hypothesis that will need further investigation.
SsrA in H. pylori: a one piece molecule induced during acid stress
Using RACE (Rapid Amplification of cDNA Ends) mapping for SsrA of H. pylori, Dong et al.  obtained two bands that were interpreted as indicative of a two-piece tmRNA like in C. crescentus and all the related α-proteobacteria , . In contrast, northern blotting experiments presented in this study indicated that SsrA is an abundant one-piece molecule in H. pylori. In cells grown at neutral pH, SsrA was detected as one band with molecular weight corresponding to the size of the mature form (386 nt). A significant increase in the amounts of SsrA was observed in H. pylori cells exposed at low pH similar to that encountered in the gastric environment. Interestingly, expression of smpB is also induced by acidity . Thus, in H. pylori the expression of the two effectors of trans-translation is higher upon acid exposure suggesting that this mechanism is enhanced under this stress condition.
We conclude that trans-translation is critical in H. pylori probably because this system is frequently required for ribosome rescue and that the associated protein tagging plays a regulatory role in the bacterial response to adverse conditions.
Finally, as more and more H. pylori strains present resistances to the commonly used antibiotics against this pathogen, we propose the essential trans-translation as an alternative specific target for the development of antibacterial drugs.
Materials and Methods
Bacterial strains and growth conditions
Escherichia coli strain MC1061 was used as a host for the preparation of plasmids employed to transform H. pylori. Antibiotics for the selection of recombinant E. coli strains were kanamycin (20 µg ml−1) or chloramphenicol (30 µg ml−1). The H. pylori strains were X47-2AL, 26695 and N6 (Table S1). H. pylori were grown as previously described . Liquid cultures were grown in Brain Heart Infusion (BHI) (Oxoid) or Brucella broth (Difco) supplemented with 0.2% β-cyclodextrin and an antibiotics/fungicide mix. For growth kinetics, we inoculated a liquid pre-culture in Brucella Broth with 1 mM IPTG (isopropyl-β-D-thiogalactoside) using 24 h old plate-grown H. pylori strains at an initial OD600nm of 0.15. Eight hours later, this preculture was diluted to a final OD 0.05, split into two flaks, one with IPTG 1 mM and one without and the cultures were followed during 35 H.
Standard procedures were as in . The QiaAmp DNA extraction kit (Qiagen) was used to extract chromosomal DNA from H. pylori. Amplifications for mutagenesis were performed using the Long Template PCR system (Roche). Oligonucleotides used for PCR amplification, site directed mutagenesis or sequencing are listed in Table S2.
Construction of H. pylori plasmids and mutants
Plasmids are listed in Table 1, strains in Table S1. pILL2150 is a H. pylori/E. coli shuttle vector carrying an inducible Ptac promoter that is functional in H. pylori . pILL786 and pILL788 were obtained by cloning wild-type smpB and ssrA genes in pILL2150, respectively. Transformation by pILL2150 and its derivates was selected on 8 µg ml−1 chloramphenicol. Deletions and point mutations were introduced in H. pylori strains by allelic exchange using suicide plasmids or three-step PCR products (as in ) in which H. pylori DNA regions corresponding to the gene to be mutated are flanking a non-polar kanamycin resistance cassette . These plasmids or PCR products were introduced in H. pylori by natural transformation and selection for mutants that had undergone double crossing-over events was performed with 20 µg ml−1 kanamycin .
Inactivation of chromosomal genes in H. pylori were performed for smpB and hp1248 (encoding RnaseR) with a three-step PCR product as in  and for ssrA with the suicide plasmid pILL796 as in . Site-directed mutagenesis was performed on pILL788 as in . Correct chromosomal insertion of a non-polar kanamycin cassette in hp1248, smpB (hp1444) and ssrA (hp0784) was verified by PCR and the introduction of point mutations in ssrA were checked by sequencing as in .
Construction of the hypB-TAP reporter gene and western blotting
To assess the efficacy of trans-translation mediated protein degradation, a reporter HypB protein fused to a TAP-tag (Tandem Affinity Purification tag) was used . HypB, a hydrogenase accessory protein encoded by hp0900, is fused with a C-terminal tag containing two tandem protein A regions that can be detected very specifically in H. pylori extracts by western blot. hypB-tap was PCR amplified from the original construction 26695-hypB-TAP  with either oligonucleotides H359/H340K that include the stop codon situated at the end of the tag sequence or with oligonucleotides H359/H341K that do not contain this stop codon. Both PCR products were cloned into pILL2150  using the SpeI-KpnI restriction sites generating plasmids pILL2322 (with stop) and pILL2323 (without stop). In a last step, using a KpnI restriction site, we added the amiF (hp1238) transcriptional terminator downstream from the fusion genes using a KpnI restriction site and obtained pILL2332 (fusion with stop and terminator) and pILL2333 (fusion without stop and with terminator), the constructions were verified by sequencing. Using three step PCR, these two fusions and the adjacent cat gene of the plasmid were amplified and introduced at the hypB chromosomal locus after transformation and chloramphenicol selection. Immunodetection of HypB-TAP proteins was performed on crude extracts migrated through SDS-PAGE with a peroxydase-coupled anti-peroxydase antibody (Sigma) as in . Intensities were quantified with the Quantity One software (Bio-Rad).
Measurement of transformation efficacy
H. pylori N6 strain harboring pILL786, pILL788, pILL791, pILL792, pILL793, pILL794, and pILL2328 (Table 1 and S1) were grown on blood agar plates, harvested after 24 h and suspended in peptone broth (Difco). Bacterial ODs were adjusted to OD 15, then 50–200 µl (approx 5×108 cells) of these preparations were spotted in duplicates on non selective plates and left to grow. Four hours later, one patch was taken for enumeration in order to determine the number of viable bacteria. One µg of plasmid DNA or PCR product was added on the other patch in order to inactivate the chromosomal copies of ssrA or smpB genes, respectively. Twelve hours later these bacteria were plated on selective media, and four days later the total numbers of transformants were counted. Transformation rates represent the number of transformants obtained per viable cell for 1 µg of DNA.
Motility tests and mouse model for colonization
H. pylori strain X47-2AL and its isogenic mutants expressing SsrADD and SsrASTOP were grown on plates for 18 h and harvested in 500 µl of peptone broth (approx OD 15). To test the motility of the strain, 2 µl of the preparations were inoculated on Brucella Broth (Difco) soft–agar plates, 0.035% Bacto-Agar (Difco), 10% (v/v) decomplemented FCS (Eurobio) by piercing the agarose. The plates were left to grow for 7 days at 37°C. Motility was measured by determining the diameter of the spread around the inoculation spot.
The in vivo colonization capacities of H. pylori strain X47-2AL and its isogenic mutants expressing SsrADD and SsrASTOP were assessed as in .
Overnight liquid cultures of wild type H. pylori strain 26695 or of the two isogenic tag- mutants SsrADD and SsrASTOP were used to inoculate BHI medium containing 10 % FCS at an initial OD of 0.15 and left to grow for 6 h. Cultures growing exponentially were used to perform the following tests. Serial dilutions of the bacteria were spotted on plates containing different concentrations of chloramphenicol (Sigma) 2 or 2.5 µg ml−1; Amoxicillin (Clamoxyl, GlaxoSmithKline) 0.2 or 0.6 µg ml−1 and plates were incubated under microaerophilic conditions. The controls consist of culture grown without these antibiotics. Counting of surviving bacteria was performed 5 days later.
To determine the sensitivity of the strains to oxidative stress, 1 ml (approx 107 bact) of cells in exponential phase were placed into 12-wells plates containing BHI supplemented with 0.5 or 0.75 µM paraquat (methyl Viologen, Sigma). Cultures were incubated at 37°C under microaerophilic conditions while shacking at 160 rpm. After 18 h, bacterial counts were performed on blood agar plates.
RNA extraction and Northern blotting
Exponentially growing liquid cultures of H. pylori wild type 26695 or isogenic mutants (OD 0.6) were centrifuged at room temperature for 10 min at 3000 g. Pellets were suspended in preheated BHI medium adjusted to pH 2.0, pH 4.5 or pH 7.0 at an OD of 0.2, left for 30 min. RNA was extracted using the phenol-chloroform method . Four µg of total RNA were separated on 4% acrylamide-urea denaturing gels, blotted onto Hybond-N+ membrane (Amersham) with a transblotter (40 min, 10 mV) and U.V. cross-linked. 5S rRNA and a 300-nucleotides-long internal fragment of ssrA 32P-labeled riboprobes were synthesized with the StripAble RNA Probe Synthesis and Removal Kit (Ambion). 5S rRNA probed on the same membranes served for calibration. Hybridization was performed at 65°C for 4 h with UltraHyb (Ambion). Quantitative analyses of blots were performed with Quantity One software (Bio-Rad).
The authors thank I. G. Boneca, D. Leduc, C. Parsot, E. Rocha and K. Schauer for helpful discussions and/or critical reading of the manuscript; N. O. Kaakhoush for his precious help in correcting our manuscript. K. Stingl for the gift of H. pylori strain with hypB-TAP fusion. We sincerely thank B. Felden for numerous suggestions and discussions. We are grateful to J. Collier and P. Bouloc for supplying strain MG1655ΔssrA and to H. Neil, K. Zemam and C. Saveanu for help in the northern blot experiments. We are very grateful to A. Labigne for her constant support. Marie Thibonnier was supported by Fondation pour la Recherche Médicale.
Conceived and designed the experiments: MT HDR. Performed the experiments: MT JMT. Analyzed the data: MT HDR. Wrote the paper: MT HDR.
- 1. Atherton JC (2006) The pathogenesis of Helicobacter pylori-induced gastro-duodenal diseases. Annu Rev Pathol 1: 63–96.JC Atherton2006The pathogenesis of Helicobacter pylori-induced gastro-duodenal diseases.Annu Rev Pathol16396
- 2. van Amsterdam K, van Vliet AHM, Kusters JG, van der Ende A (2006) Of microbe and man: determinants of Helicobacter pylori-related diseases. FEMS Microbiol Rev 30: 131–156.K. van AmsterdamAHM van VlietJG KustersA. van der Ende2006Of microbe and man: determinants of Helicobacter pylori-related diseases.FEMS Microbiol Rev30131156
- 3. Wang G, Alamuri P, Maier RJ (2006) The diverse antioxidant systems of Helicobacter pylori. Mol Microbiol 61: 847–860.G. WangP. AlamuriRJ Maier2006The diverse antioxidant systems of Helicobacter pylori.Mol Microbiol61847860
- 4. Keiler K, Waller P, Sauer RT (1996) Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA. Science 271: 990–993.K. KeilerP. WallerRT Sauer1996Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA.Science271990993
- 5. Dulebohn D, Choy J, Sundermeier T, Okan N, Karzai AW (2007) Trans-translation: the tmRNA-mediated surveillance mechanism for ribosome rescue, directed protein degradation, and nonstop mRNA decay. Biochemistry 46: 4681–4693.D. DulebohnJ. ChoyT. SundermeierN. OkanAW Karzai2007Trans-translation: the tmRNA-mediated surveillance mechanism for ribosome rescue, directed protein degradation, and nonstop mRNA decay.Biochemistry4646814693
- 6. Moore SD, Sauer RT (2007) The tmRNA system for translational surveillance and ribosome rescue. Annu Rev Biochem 76: 101–124.SD MooreRT Sauer2007The tmRNA system for translational surveillance and ribosome rescue.Annu Rev Biochem76101124
- 7. Keiler KC (2008) Biology of trans-Translation. Ann Rev Microbiol 62: 133–151.KC Keiler2008Biology of trans-Translation.Ann Rev Microbiol62133151
- 8. Gottesman S, Roche ED, Zhou Y, Sauer RT (1998) The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system. Genes Dev 9: 1338–1347.S. GottesmanED RocheY. ZhouRT Sauer1998The ClpXP and ClpAP proteases degrade proteins with carboxy-terminal peptide tails added by the SsrA-tagging system.Genes Dev913381347
- 9. Herman C, Thévenet D, Bouloc P, Walker GC, D'Ari R (1998) Degradation of carboxy-terminal-tagged cytoplasmic proteins by the Escherichia coli protease HflB (FtsH). Genes Dev 1348–1355.C. HermanD. ThévenetP. BoulocGC WalkerR. D'Ari1998Degradation of carboxy-terminal-tagged cytoplasmic proteins by the Escherichia coli protease HflB (FtsH).Genes Dev13481355
- 10. Richards J, Mehta P, Karzai WA (2006) RNase R degrades non-stop mRNAs selectively in an SmpB-tmRNA-dependent manner. Mol Microbiol 62: 1700–1712.J. RichardsP. MehtaWA Karzai2006RNase R degrades non-stop mRNAs selectively in an SmpB-tmRNA-dependent manner.Mol Microbiol6217001712
- 11. Moore SD, Sauer RT (2005) Ribosome rescue: tmRNA activity and capacity in Escherichia coli. Mol Microbiol 58: 456–466.SD MooreRT Sauer2005Ribosome rescue: tmRNA activity and capacity in Escherichia coli.Mol Microbiol58456466
- 12. Hong S-J, Lessner FH, Mahen EM, Keiler KC (2007) Proteomic identification of tmRNA substrates. Proc Nat Acad Sci 104: 17128–17133.S-J HongFH LessnerEM MahenKC Keiler2007Proteomic identification of tmRNA substrates.Proc Nat Acad Sci1041712817133
- 13. Abo T, Ueda K, Sunohara T, Ogawa K, Aiba H (2002) SsrA-mediated protein tagging in the presence of miscoding drugs and its physiological role in Escherichia coli. Genes Cells 7: 629–638.T. AboK. UedaT. SunoharaK. OgawaH. Aiba2002SsrA-mediated protein tagging in the presence of miscoding drugs and its physiological role in Escherichia coli.Genes Cells7629638
- 14. Ougland R, Zhang C-M, Liiv A, Johansen RF, Seeberg E, et al. (2004) AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation. Mol Cell 16: 107–116.R. OuglandC-M ZhangA. LiivRF JohansenE. Seeberg2004AlkB restores the biological function of mRNA and tRNA inactivated by chemical methylation.Mol Cell16107116
- 15. Falnes PO (2005) RNA repair: the latest addition to the toolbox for macromolecular maintenance. RNA Biol 2: 14–16.PO Falnes2005RNA repair: the latest addition to the toolbox for macromolecular maintenance.RNA Biol21416
- 16. Keiler KC (2007) Physiology of tmRNA: what gets tagged and why? Curr Opin Microbiol 10: 169–175.KC Keiler2007Physiology of tmRNA: what gets tagged and why?Curr Opin Microbiol10169175
- 17. Oh BK, Apirion D (1991) 10Sa RNA, a small stable RNA of Escherichia coli, is functional. Mol Gen Genet 229: 52–56.BK OhD. Apirion199110Sa RNA, a small stable RNA of Escherichia coli, is functional.Mol Gen Genet2295256
- 18. Hallier M, Ivanova N, Rametti A, Pavlov M, Ehrenberg M, et al. (2004) Pre-binding of small protein B to a stalled ribosome triggers trans-translation. J Biol Chem 279: 25978–25985.M. HallierN. IvanovaA. RamettiM. PavlovM. Ehrenberg2004Pre-binding of small protein B to a stalled ribosome triggers trans-translation.J Biol Chem2792597825985
- 19. Muto A, Fujihara A, Ito K-i, Matsuno J, Ushida C, et al. (2000) Requirement ot transfert-messenger RNA for growth of Bacillus subtilis under stresses Genes Cells 8: 627–635.A. MutoA. FujiharaK-i ItoJ. MatsunoC. Ushida2000Requirement ot transfert-messenger RNA for growth of Bacillus subtilis under stressesGenes Cells8627635
- 20. Vioque A, de la Cruz J (2003) Trans-translation and protein synthesis inhibitors. FEMS Microbiol Lett 218: 9–14.A. VioqueJ. de la Cruz2003Trans-translation and protein synthesis inhibitors.FEMS Microbiol Lett218914
- 21. Luidalepp H, Hallier M, Felden B, Tenson T (2005) tmRNA decreases the bactericidal activity of aminoglycosides and the susceptibility to inhibitors of cell wall synthesis. RNA Biol 2: 70–74.H. LuidaleppM. HallierB. FeldenT. Tenson2005tmRNA decreases the bactericidal activity of aminoglycosides and the susceptibility to inhibitors of cell wall synthesis.RNA Biol27074
- 22. Julio SM, Heithoff DM, Mahan MJ (2000) ssrA (tmRNA) plays a role in Salmonella enterica Serovar Typhimurium pathogenesis. J Bacteriol 182: 1558–1563.SM JulioDM HeithoffMJ Mahan2000ssrA (tmRNA) plays a role in Salmonella enterica Serovar Typhimurium pathogenesis.J Bacteriol18215581563
- 23. Bäumler AJ, Kusters GJ, Stojiljkovic I, Heffron F (1994) Salmonella typhimurium loci involved in survival within macrophages. Infect Immun 62: 1623–1630.AJ BäumlerGJ KustersI. StojiljkovicF. Heffron1994Salmonella typhimurium loci involved in survival within macrophages.Infect Immun6216231630
- 24. Okan NA, Bliska JB, Karzai AW (2006) A Role for the SmpB-SsrA system in Yersinia pseudotuberculosis pathogenesis. PLoS Pathogens 2: e6.NA OkanJB BliskaAW Karzai2006A Role for the SmpB-SsrA system in Yersinia pseudotuberculosis pathogenesis.PLoS Pathogens2e6
- 25. Keiler KC, Shapiro L (2003) tmRNA in Caulobacter crescentus is cell cycle regulated by temporally controlled transcription and RNA degradation. J Bacteriol 185: 1825–1830.KC KeilerL. Shapiro2003tmRNA in Caulobacter crescentus is cell cycle regulated by temporally controlled transcription and RNA degradation.J Bacteriol18518251830
- 26. Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, et al. (2006) Essential genes of a minimal bacterium. Proc Natl Acad Sci U S A 103: 425–430.JI GlassN. Assad-GarciaN. AlperovichS. YoosephMR Lewis2006Essential genes of a minimal bacterium.Proc Natl Acad Sci U S A103425430
- 27. French CT, Lao P, Loraine AE, Matthews BT, Yu H, et al. (2008) Large-scale transposon mutagenesis of Mycoplasma pulmonis. Mol Microbiol 69: 67–76.CT FrenchP. LaoAE LoraineBT MatthewsH. Yu2008Large-scale transposon mutagenesis of Mycoplasma pulmonis.Mol Microbiol696776
- 28. Akerley BJ, Rubin EJ, Novick VL, Amaya K, Judson N, et al. (2002) A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc Natl Acad Sci U S A 99: 966–971.BJ AkerleyEJ RubinVL NovickK. AmayaN. Judson2002A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae.Proc Natl Acad Sci U S A99966971
- 29. Huang C, Wolfgang MC, Withey J, Koomey M, Friedman DI (2000) Charged tmRNA but not tmRNA-mediated proteolysis is essential for Neisseria gonorrhoeae viability. EMBO J 19: 1098–1107.C. HuangMC WolfgangJ. WitheyM. KoomeyDI Friedman2000Charged tmRNA but not tmRNA-mediated proteolysis is essential for Neisseria gonorrhoeae viability.EMBO J1910981107
- 30. Boneca IG, Ecobichon C, Chaput C, Mathieu A, Guadagnini S, et al. (2008) Development of inducible systems to engineer conditional mutants of essential genes of Helicobacter pylori. Appl Environ Microbiol 74: 2095–2102.IG BonecaC. EcobichonC. ChaputA. MathieuS. Guadagnini2008Development of inducible systems to engineer conditional mutants of essential genes of Helicobacter pylori.Appl Environ Microbiol7420952102
- 31. Hanawa-Suetsugu K, Takagi M, Inokuchi H, Himeno H, Muto A (2002) SmpB functions in various steps of trans-translation. Nucl Acids Res 30: 1620–1629.K. Hanawa-SuetsuguM. TakagiH. InokuchiH. HimenoA. Muto2002SmpB functions in various steps of trans-translation.Nucl Acids Res3016201629
- 32. Hanawa-Suetsugu K, Bordeau V, Himeno H, Muto A, Felden B (2001) Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging. Nucl Acids Res 29: 4663–4673.K. Hanawa-SuetsuguV. BordeauH. HimenoA. MutoB. Felden2001Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging.Nucl Acids Res2946634673
- 33. Komine Y, Kitabatake M, Yokogawa T, Nishikawa K, Inokuchi H (1994) A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli. Proc Nat Acad Sci 91: 9223–9227.Y. KomineM. KitabatakeT. YokogawaK. NishikawaH. Inokuchi1994A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli.Proc Nat Acad Sci9192239227
- 34. Stingl K, Schauer K, Ecobichon C, Labigne A, Lenormand P, et al. (2008) In vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification. Mol Cell Proteomics In press. K. StinglK. SchauerC. EcobichonA. LabigneP. Lenormand2008In vivo interactome of Helicobacter pylori urease revealed by tandem affinity purification.Mol Cell Proteomics In press
- 35. Skouloubris S, Labigne A, De Reuse H (2001) The AmiE aliphatic amidase and AmiF formamidase of Helicobacter pylori: natural evolution of two enzyme paralogs. Molec Microbiol 40: 596–609.S. SkouloubrisA. LabigneH. De Reuse2001The AmiE aliphatic amidase and AmiF formamidase of Helicobacter pylori: natural evolution of two enzyme paralogs.Molec Microbiol40596609
- 36. Bury-Moné S, Thiberge J-M, Contreras M, Maitournam A, Labigne A, et al. (2004) Responsiveness to acidity via metal ion regulators mediates virulence in the gastric pathogen Helicobacter pylori. Mol Microbiol 53: 623–638.S. Bury-MonéJ-M ThibergeM. ContrerasA. MaitournamA. Labigne2004Responsiveness to acidity via metal ion regulators mediates virulence in the gastric pathogen Helicobacter pylori.Mol Microbiol53623638
- 37. Suerbaum S, Josenhans C (2007) Helicobacter pylori evolution and phenotypic diversification in a changing host. Nature Rev Microbiol 5: 441–452.S. SuerbaumC. Josenhans2007Helicobacter pylori evolution and phenotypic diversification in a changing host.Nature Rev Microbiol5441452
- 38. Ivanova N, Pavlov MY, Ehrenberg M (2005) tmRNA-induced release of messenger RNA from stalled ribosomes. J Mol Biol 350: 897–905.N. IvanovaMY PavlovM. Ehrenberg2005tmRNA-induced release of messenger RNA from stalled ribosomes.J Mol Biol350897905
- 39. Lessner FH, Venters BJ, Keiler KC (2007) Proteolytic adaptor for transfer-messenger RNA-tagged proteins from α-proteobacteria. J Bacteriol 189: 272–275.FH LessnerBJ VentersKC Keiler2007Proteolytic adaptor for transfer-messenger RNA-tagged proteins from α-proteobacteria.J Bacteriol189272275
- 40. Tomb J-F, White O, Kerlavage AR, Clayton RA, Sutton GG, et al. (1997) The complete genome sequence of the gastric pathogen Helicobacter pylori. Nature 388: 539–547.J-F TombO. WhiteAR KerlavageRA ClaytonGG Sutton1997The complete genome sequence of the gastric pathogen Helicobacter pylori.Nature388539547
- 41. de la Cruz J, Vioque A (2001) Increased sensitivity to protein synthesis inhibitors in cells lacking tmRNA. RNA 12: 1708–1716.J. de la CruzA. Vioque2001Increased sensitivity to protein synthesis inhibitors in cells lacking tmRNA.RNA1217081716
- 42. Kohanski MA, Dwyer DJ, Hayete B, Lawrence CA, Collins JJ (2007) A common mechanism of cellular death induced by bactericidal antibiotics. Cell 130: 797–810.MA KohanskiDJ DwyerB. HayeteCA LawrenceJJ Collins2007A common mechanism of cellular death induced by bactericidal antibiotics.Cell130797810
- 43. Kaakoush NO, Kovach Z, Mendz GL (2007) Potential role of thiol:disulfide oxidoreductases in the pathogenesis of Helicobacter pylori. FEMS Immunol Med Microbiol 50: 177–183.NO KaakoushZ. KovachGL Mendz2007Potential role of thiol:disulfide oxidoreductases in the pathogenesis of Helicobacter pylori.FEMS Immunol Med Microbiol50177183
- 44. Karnholz A, Hoefler C, Odenbreit S, Fischer W, Hofreuter D, et al. (2006) Functional and Topological Characterization of Novel Components of the comB DNA Transformation Competence System in Helicobacter pylori. J Bacteriol 188: 882–893.A. KarnholzC. HoeflerS. OdenbreitW. FischerD. Hofreuter2006Functional and Topological Characterization of Novel Components of the comB DNA Transformation Competence System in Helicobacter pylori.J Bacteriol188882893
- 45. Dong Q, Zhang L, Goh K-l, Forman D, O'Rourke J, et al. (2007) Identification and characterisation of ssrA in members of the Helicobacter genus. Antonie van Leeuwenhoek 92: 301–307.Q. DongL. ZhangK-l GohD. FormanJ. O'Rourke2007Identification and characterisation of ssrA in members of the Helicobacter genus.Antonie van Leeuwenhoek92301307
- 46. Keiler KC, Shapiro L, Williams KP (2000) tmRNAs that encode proteolysis-inducing tags are found in all known bacterial genomes: A two-piece tmRNA functions in Caulobacter. Proc Nat Acad Sci 97: 7778–7783.KC KeilerL. ShapiroKP Williams2000tmRNAs that encode proteolysis-inducing tags are found in all known bacterial genomes: A two-piece tmRNA functions in Caulobacter.Proc Nat Acad Sci9777787783
- 47. Bury-Moné S, Skouloubris S, Labigne A, De Reuse H (2001) The Helicobacter pylori UreI protein : role in adaptation to acidity and identification of residues essential for its activity and for acid activation. Mol Microbiol 42: 1021–1034.S. Bury-MonéS. SkouloubrisA. LabigneH. De Reuse2001The Helicobacter pylori UreI protein : role in adaptation to acidity and identification of residues essential for its activity and for acid activation.Mol Microbiol4210211034
- 48. Sambrook J, Russel DW (2001) Molecular Cloning: a Laboratory Manual: Cold Spring Harbor LaboratoryJ. SambrookDW Russel2001Molecular Cloning: a Laboratory Manual: Cold Spring Harbor Laboratory
- 49. Stingl K, Brandt S, Uhlemann E-M, Schmid R, Altendorf K, et al. (2007) Channel-mediated potassium uptake in Helicobacter pylori is essential for gastric colonization. Embo J 26: 232–241.K. StinglS. BrandtE-M UhlemannR. SchmidK. Altendorf2007Channel-mediated potassium uptake in Helicobacter pylori is essential for gastric colonization.Embo J26232241
- 50. Tu G-F, Reid GE, Zhang J-G, Moritz RL, Simpson RJ (1995) C-terminal extension of truncated recombinant proteins in Escherichia coli with a 10Sa RNA decapeptide. J Biol Chem 270: 9322–9326.G-F TuGE ReidJ-G ZhangRL MoritzRJ Simpson1995C-terminal extension of truncated recombinant proteins in Escherichia coli with a 10Sa RNA decapeptide.J Biol Chem27093229326
- 51. Ebeling S, Kundig C, Hennecke H (1991) Discovery of a rhizobial RNA that is essential for symbiotic root nodule development. J Bacteriol 173: 6373–6382.S. EbelingC. KundigH. Hennecke1991Discovery of a rhizobial RNA that is essential for symbiotic root nodule development.J Bacteriol17363736382
- 52. Keiler KC, Shapiro L (2003) tmRNA is required for correct timing of DNA replication in Caulobacter crescentus. J Bacteriol 185: 573–580.KC KeilerL. Shapiro2003tmRNA is required for correct timing of DNA replication in Caulobacter crescentus.J Bacteriol185573580
- 53. Braud S, Lavire C, Bellier A, Mazodier P (2006) Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces. Arch Microbiol 184: 343–352.S. BraudC. LavireA. BellierP. Mazodier2006Effect of SsrA (tmRNA) tagging system on translational regulation in Streptomyces.Arch Microbiol184343352
- 54. Casadaban M, Cohen SN (1980) Analysis of gene control signals by DNA fusions and cloning in E. coli. J Mol Biol 138: 179–207.M. CasadabanSN Cohen1980Analysis of gene control signals by DNA fusions and cloning in E. coli.J Mol Biol138179207
- 55. Blattner FR, Plunkett G III, Bloch CA, Perna NT, Burland V, et al. (1997) The complete genome sequence of Escherichia coli K-12. Science 277: 1453–1462.FR BlattnerG. Plunkett IIICA BlochNT PernaV. Burland1997The complete genome sequence of Escherichia coli K-12.Science27714531462
- 56. Ferrero RL, Cussac V, Courcoux P, Labigne A (1992) Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange. J Bacteriol 174: 4212–4217.RL FerreroV. CussacP. CourcouxA. Labigne1992Construction of isogenic urease-negative mutants of Helicobacter pylori by allelic exchange.J Bacteriol17442124217
- 57. Ermak TH, Giannasca PJ, Nichols R, Myers GA, Nedrud J, et al. (1998) Immunization of mice with urease vaccine affords protection against Helicobacter pylori infection in the absence of antibodies and is mediated by MHC class II-restricted response. J Exp Med 188: 2277–2288.TH ErmakPJ GiannascaR. NicholsGA MyersJ. Nedrud1998Immunization of mice with urease vaccine affords protection against Helicobacter pylori infection in the absence of antibodies and is mediated by MHC class II-restricted response.J Exp Med18822772288