Although uterine macrophages are thought to play an important regulatory role at the maternal-fetal interface, their global gene expression profile is not known.
Using micro-array comprising approximately 14,000 genes, the gene expression pattern of human first trimester decidual CD14+ monocytes/macrophages was characterized and compared with the expression profile of the corresponding cells in blood. Some of the key findings were confirmed by real time PCR or by secreted protein. A unique gene expression pattern intrinsic of first trimester decidual CD14+ cells was demonstrated. A large number of regulated genes were functionally related to immunomodulation and tissue remodelling, corroborating polarization patterns of differentiated macrophages mainly of the alternatively activated M2 phenotype. These include known M2 markers such as CCL-18, CD209, insulin-like growth factor (IGF)-1, mannose receptor c type (MRC)-1 and fibronectin-1. Further, the selective up-regulation of triggering receptor expressed on myeloid cells (TREM)-2, alpha-2-macroglobulin (A2M) and prostaglandin D2 synthase (PGDS) provides new insights into the regulatory function of decidual macrophages in pregnancy that may have implications in pregnancy complications.
Citation: Gustafsson C, Mjösberg J, Matussek A, Geffers R, Matthiesen L, Berg G, et al. (2008) Gene Expression Profiling of Human Decidual Macrophages: Evidence for Immunosuppressive Phenotype. PLoS ONE 3(4): e2078. https://doi.org/10.1371/journal.pone.0002078
Editor: Derya Unutmaz, New York University School of Medicine, United States of America
Received: February 20, 2008; Accepted: March 21, 2008; Published: April 30, 2008
Copyright: © 2008 Gustafsson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by grants from the Swedish Research Council, the Medical Research Council of South-east Sweden (FORSS), the County Council of Östergötland and by a grant from the National Institutes of Health, USA (HD-41701).
Competing interests: The authors have declared that no competing interests exist.
A distinctive immune tolerance environment is programmed at the maternal-fetal interface to avoid rejection by the maternal immune system. The most abundant maternal immune cells in the decidualized endometrium are NK cells and macrophages. Although their exact roles are currently being debated, they are thought to regulate vascular growth and placenta development. Global gene expression profiling of decidual NK cells provided a unique pattern compared to their counterparts in blood . In the case of monocytes/macrophages, accumulating data indicate that local environment drives them into functionally different subsets. Classically, macrophages activated by agents like IFN-γ, TNF or bacterial LPS, show profound effector functions like production of toxic intermediates, essential in the killing of intracellular microbes. They also secrete high amounts of pro-inflammatory and Th1 cytokines such as TNF and IL-12. On the other hand, macrophages activated by Th2 cytokines such as IL-4 and IL-13 as well as anti-inflammatory cytokine IL-10 induce a suppressive mode of activation and have been termed alternatively activated macrophages (, reviewed in , ). In this regard, Mantovani et al  proposed a nomenclature where M1 and M2 represent blood derived macrophages activated by inflammatory and anti-inflammatory cytokines, respectively. However, it is not clear whether decidual macrophages represent mainly M1 or M2 phenotype.
Due to their role in immune regulation, macrophages in the uterus are likely to contribute to local immune tolerance in normal pregnancy. They may do so by phagocytosis of apoptotic cells harbouring potentially pro-inflammatory and harmful effects on the fetus (reviewed in ). Temporal expression of anti-inflammatory cytokines such as IL-4 –, IL-13 – and IL-10 –, ,  is evident at the maternal-fetal interface, suggesting an environment capable of facilitating alternative activation of macrophages. It is thus likely that decidual macrophages reveal a suppressive or regulatory phenotype in the placenta ,  as well as in the decidua , –. We report here, for the first time, a global gene expression pattern of decidual monocytes/macrophages. We compared them with the expression patterns of blood CD14 positive cells. A clearly distinct gene expression profile in decidual macrophages is evident, confirming a selective gene cascade regulation involved in immune modulation and tissue remodelling.
Materials and Methods
Decidual tissue and blood was obtained from eleven women between 18 and 41 years of age (median age 35 years) with normal pregnancies, undergoing elective surgical abortions in week 7 to 11 (median week 9) at the department of Gynecology and Obstetrics, Linköping University Hospital, Sweden. All pregnancies were detected viable and dated by crown-rump length measurement with ultrasound. Misoprostol (Cytotec®; Searle, USA) was given to four of the eleven subjects before the procedure; the data from these subjects did not differ from the other subjects. Laboratory analyses performed in the different subjects are shown in table 1. Written informed consent was obtained from all subjects. The study was approved by the Local Ethics Committee of Linköping University.
Isolation of CD14 positive cells and extraction of total RNA
Mononuclear cells were separated from decidua and blood as described previously . CD14 positive cells were obtained by two different methods. Cells from five subjects were sorted by FACSAria cell sorting, a method resulting in high cell purity but with the possible disadvantage of causing high mechanical stress to the cells. Positive immunomagnetic MACS separation, a fast method with a relatively low physical impact on the cells, was performed in the six remaining subjects.
For immunomagnetic separation, CD14 positive cells were selected with anti-CD14 antibody coated beads (Miltenyi Biotec, Bergish Gladbach, Germany) according to the manufacturer's instructions. Flow cytometry in 3 of 6 samples showed purity (% CD14+ cells) of 84, 88 and 92 in blood and 72, 74 and 76 in the decidua, respectively. For FACSAria (BD Bioscience) cell sorting, cells were labelled with anti-CD4FITC, CD14APC (Miltenyi Biotech), CD45PE-Cy5 and CD3 PE-Cy7 antibodies (BD Bioscience, Stockholm, Sweden). CD14 positive cells were defined as CD45+CD3−CD14+ cells and sorted accordingly. Purity of FACSAria sorted CD14 positive cells in the two subjects used for micro-array was 98.0 to 99.8% in blood and 96.2–97.2% in decidua, respectively. In the three subjects used for real-time PCR, purity was 93–98% in blood and 86–96% in the decidua, respectively. Following sorting, cells were lysed in RNeasy RLT lysing buffer and total RNA was extracted according to the manufacturer's instructions (Qiagen, West Sussex, UK).
Affymetrix GeneChip Assay
Samples were amplified for GeneChip analysis according to the recommended protocols by the manufacturer (Affymetrix, Santa Clara, CA, USA). In all cases, 10 µg of each biotinylated cRNA preparation was fragmented and placed in a hybridization cocktail containing four biotinylated hybridization controls (BioB, BioC, BioD, and Cre), as recommended by the manufacturer. Samples were hybridized to an identical lot of Affymetrix HG U133 2.0 GeneChips for 16 hours. After hybridization the GeneChips were washed, stained with SA-PE, and read using an Affymetrix GeneChip fluidic station and scanner.
Analysis of micro-array data was performed using the Affymetrix GCOS 1.2 software. For normalization all array experiments were scaled to a target intensity of 150, otherwise using the default values of GCOS 1.2. Further downstream analysis was performed using Array Assist 4.0 (Stratagene, La Jolla, CA, USA). Data was normalized by the PLIER algorithm (Affymetrix, Santa Clara, CA, USA) using default parameters. Genes whose signal maximum intensity did not exceed 100 across all samples were excluded from further analysis. Student's T-Test was applied to identify differences between decidual and blood monocytes/macrophages. Genes whose p values were below or equal to 0.05 and mean fold changes more/less than +2/−2 fold in cells from two of the high purity FACSAria separated subjects were considered differentially regulated in decidual samples compared with blood cells. These genes were used as a gene expression signature for the experiment, resulting in 408 regulated genes. Among these, genes fulfilling the same criteria in cells from the five MACS separated subjects were selected and resulted in the final 120 regulated genes. Alongside with these high-stringent requirements, data analysis with lower stringency was performed. With the requirement of two-fold increase/decrease and statistical difference in all seven subjects considered as a common group with no respect to the separation method used, a much higher number of genes were revealed regulated (n∼1700). We chose to further analyse the genes obtained by the high-stringency requirements. These genes were grouped according to plausible functions in the context of macrophages in early pregnancy, resulting in the groups immune modulation, tissue remodelling, cell cycle related and cell metabolism/transport.
To confirm regulated genes in the micro-array, real-time PCR was performed on flow-cytometrically sorted CD14 positive cells obtained from three additional subjects. RNA was reversely transcribed with 500 U SuperScript II reverse transcriptase (Invitrogen, Carlsbad, California) according to the manufactureŕs protocol. Table 2 provides all genes with corresponding primers (synthesized by TIB Molbiol, Berlin, Germany) used for real-time PCR. Relative quantification was performed using the 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, California) under the following conditions: 10 min at 95 °C followed by 40 cycles at 95°C for 15seconds and 60 seconds at 60°C. Amplification of specific PCR products was detected using the SYBR Green PCR Master Mix (Applied Biosystems) in duplicates. The housekeeping ribosomal protein S9 (RPS9) gene was used for normalization. The number of genes analyzed was restricted by the low amount of lysate available.
Analysis of ex vivo protein secretion from CD14 positive cells
Blood and decidual immunomagnetically separated CD14 positive cells (50 000 cells) from five subjects were incubated over night in 200 µL of tissue culture medium (TCM) consisting of Iscoves modification of Dulbecco's media (Gibco BRL, Paisley, Scotland) supplemented with (given as final concentrations in the medium): L-glutamine (Flow Laboratories, Irvine, Scotland) 292 mg/L; sodium bicarbonate 3.024 g/L; penicillin 50 IE/mL and streptomycin 50 µg/mL (Flow Laboratories) and 100 x non essential amino acids 10 mL/L (Flow Laboratories). The cell-free supernatants were stored at −70°C. The protein level of CCL18 was determined by an in-house double-antibody sandwich ELISA using a monoclonal anti-human CCL18 (0.5 µg/mL;clone 64507; R&D Systems, Abingdon, UK) and a polyclonal biotinylated anti-human CCL18 Antibody (200 ng/ml; R&D Systems). Streptavidin-poly-horse radish peroxidase and 3,3′,5,5′-tetramethylbenzidine liquid substrate (Sigma-Aldrich) were used for detection (Sandberg et al, unpublished results). The levels of CCL2 and matrix metalloproteinase (MMP)-9 in cell culture supernatants were analyzed according to the manufacturer's instructions. The kits used were base kits LUH000 and LMP000 together with analyte kits LUH279 and LMP911 (all purchased fromR&D Systems, Abingdon, UK). Protein concentrations were analyzed using the Luminex 100 instrument (Luminex Corporation, Austin, Texas, USA) and the STarStation software (v 2.3, Applied cytometry Systems, Sheffield, UK). Genes were selected for protein analyses based on significance and possibility of detection due to availability of assay.
Gene expression data revealed a total of 120 genes differently regulated (80 up-regulated and 40 down-regulated) in decidual CD14 positive cells compared to their blood counterparts, when using the most stringent statistical criteria. A complete list of differentially regulated genes is provided as supplementary material and a selection is summarized in Table 3. A major group of genes encode for proteins with immune regulatory functions. Further, genes associated with tissue remodelling, cell proliferation and cell metabolism were commonly regulated. Figure 1 shows a cluster picture of the gene signature of 408 regulated genes.
Requirements for regulation was 2-fold up-/down-regulation and a p-value <0.05 in the two subjects with highly pure cellpopulations (subjects 1 and 2).
Real-time PCR for confirmation of mRNA expression
Real-time PCR was used to further confirm the expression of three genes differentially expressed in the array, triggering receptor expressed on myeloid cells (TREM-2), CD209 and ICAM-3. Further, two genes, indoleamine 2,3-dioxygenase (IDO) and neuropilin (NRP-1), with two-fold regulation in all seven subjects but just below statistical significance were analyzed. PCR results showed that these five genes were differently regulated in decidual CD14 positive cells compared to cells from blood (Figure 2).
Luminex and ELISA for confirmation of protein secretion
Altered gene expression observed in the array analysis was also studied at the protein level by assaying 24-hour cell culture supernatant of CD14 positive macrophages. Decidual CD14 positive cells secreted significantly higher levels of CCL-18, CCL2 and MMP-9 compared to blood monocytes/macrophages, in agreement with the array data (Figure 3).
The maternal-fetal interface presents a unique immune site with the decidua populated with numerous and specialized NK cells and macrophages. Here we report a distinct and well characterized micro-array analysis of human decidual macrophage gene expression, comprising immunomodulatory and tissue remodelling genes, as well as genes related to cell proliferation and metabolism. While only few up-regulated genes were signature of classically activated M1 macrophage phenotype –, several of the regulated genes corresponded to markers of alternatively activated macrophages, including CCL18 , CD209 , mannose receptor C type (MRC)-1 , fibronectin-1  and insulin-like growth factor (IGF)-1 . All of these were recently shown to be up-regulated in blood derived M2 macrophages in a gene expression comparison with M1 polarized macrophages . In addition, a number of the differentially regulated genes have previously been assigned to decidual macrophages and are confirmed by our data. We also report evidence of other markers connected to alternative macrophage activation, e.g. TREM-2, alpha 2 macroglobulin (A2M) and prostaglandin D2 synthase (PGDS).
Among the up-regulated genes a major group could be classified as immune modulatory with immuno-suppressive or anti-inflammatory functions. In agreement with this, a group of activating or pro-inflammatory genes were down-regulated in decidual macrophages compared to their blood counterparts. Several of the up-regulated genes are surface receptors, among these the often co-expressed lectins MRC-1 (CD206, macrophage mannose receptor; MMR) and CD209 as well as the tetraspanin CD9. All have previously been detected in uterine cells in human pregnancy , –. MRC1 and CD209 (dendritic cell specific intercellular adhesion molecule-grabbing nonintegrin; DC-SIGN) are generally associated with alternative activation profile of macrophages. NRP-1, involved in establishment of the immunological synapse, is a receptor connected to regulatory T cells . NRP-1 is up-regulated in decidual macrophages and was recently shown to be up-regulated in M2 cells , indicating a potential role in macrophage polarization.
TREM-2 expression, to our knowledge, has not been previously reported in uterine tissue or in relation to pregnancy. In this regard, we propose a novel mechanism of potential importance of TREM-2 in maternal-fetal tolerance. In mouse macrophages, TREM-2 is induced by IL-4 and is down-regulated by IFN-γ or LPS . Information on TREM-2 in humans is very limited, although it was found to be down-regulated in LPS-stimulated human monocyte-derived dendritic cells (DCs) . Murine macrophages express a ligand for TREM-2 on their surface thus enabling auto-regulation . High expression of TREM-2 in mouse microglial cells correlate with their ability to phagocytose apoptotic neurons, hence TREM-2 might be positively regulating phagocytosis but negatively regulating inflammatory responses . This proposed dual function of TREM-2 positive cells would fit well in the context of the pregnant uterus, where the need for both immune regulation and apoptotic clearance is high.
Chemokines play a central role in polarization of macrophages. CCL2 and CCL18 were found to be up-regulated, the latter being involved in recruitment and possibly tolerance indcuction of naïve T cells . Interestingly, CCL18 was found to be expressed in human decidual and amnion tissues during pregnancy whereas expression was reduced after onset of labour . On the other hand, CCL2 (monocyte chemoattractant protein-1; MCP-1) is classically linked with inflammatory responses (reviewed in ). However, CCL2 was shown to drive Th2 polarization in mice and stimulation with IL-4/IL-13 in human lung epithelial cells increased their secretion of CCL2 . Daly et al hypothesized a dual effect of CCL2 depending on environmental demands . We therefore suggest a role for CCL2 in pregnancy in recruiting monocytes/macrophages to the decidua, where other local factors will be decisive for polarization. PGDS is another up-regulated soluble immunemodulating molecuble that has been associated with decidual Th2 cell recruitment and antigen presentation . IDO, a tryptophan depleting enzyme, was ascribed a role in murine pregnancy . Although the role of IDO at the maternal-fetal interface has been questioned , its expression in decidual macrophages has previously been noted , thus a finding confirmed in this study.
Considering the plasticity and growth of the feto-placental unit, functions related to angiogenesis and depletion of apoptotic cells are important for a successful pregnancy. Accordingly, a large group of genes up-regulated in decidual CD14 positive cells was associated with tissue remodelling. Fibronectin increases apoptotic clearance of cells coated e.g. with C1Qs, both molecules being up-regulated here. We also found up-regulation of collagen genes as well as of other molecules promoting clearance or angiogenesis, e.g. growth arrest-specific (GAS)-6 and protein S alpha (PROS1). Although MMP9 (plasminogen) is considered a proinflammatory chemokine, it also cleaves denatured collagens and type IV collagens in basement membranes, thereby contributing to remodelling of extracellular matrix and migration of immune cells. A2M regulates functions of cytokines but in pregnancy it is suggested to particularly be involved in tissue remodelling during trophoblast invasion , . IGF-I is an angiogenic factor that affects fetal nutrition and size (reviewed in  and its expression is associated with alternatively activated macrophages , , in agreement with the angiogenic characteristics of this cell type. We here confirm the role of uterine macrophages in tissue remodelling and angiogenesis during placental invasion , , and extend knowledge by adding a number of regulated genes involved in tissue remodelling.
A large group of regulated genes was related to cell cycle functions. Taking into consideration the balance between up- and down-regulated genes, the net effect was a clear-cut signature of proliferation, which is in line with the previously demonstrated proliferative capacity of macrophages , . Our results also closely resemble the array data from Martinez et al  where many genes associated with cell proliferation were up-regulated in macrophage differentiation and priming of M1 as well of M2.Genes related to cell metabolism and transport were common both in the up- and down-regulated groups of genes. This is in line with Martinez et al where for example lipid metabolites and solute carriers were regulated in differentiation and priming of macrophages .
While the present study focused on analysis of the gene expression profile, previous studies have reported cell surface expression of CD14+ decidual macrophages to include CD209 , , HLA-DR ,  and CD68 , whereas the relative expression of CD80, CD83 and CD86 was lowered , , indicating a potential to induce T-cell anergy. Previous studies have also shown a suppression of mixed leukocyte reaction (MLR) as well as of mitogen-induced proliferation of decidual macrophages as compared with their blood counterparts, thus linking decidual macrophages to a functional suppressive phenotype .
Macrophages show a high degree of plasticity and they adapt to the tissue environment , often developing properties that do not precisely fit into the in vitro generated M1 or M2 profiles, e.g. macrophages in lung tissue  or in tumors (reviewed in ). Although decidual macrophages mainly fall into the M2 category, it is important to note that they do exhibit a unique profile. The precise requirements for this polarization remain to be settled, but local cytokines and hormones are likely to have important roles. We also speculate for a role of hepatocyte growth factor (HGF) since the gene expression profile of monocytes cultured with HGF  resembled that of decidual CD14 positive cells reported here. HGF is normally expressed at high levels in the placenta whereas low levels are associated with pregnancy complications –. Polarized macrophages do not represent stable lineages, but rather show reversible adaptations to changes of the environment , , . Thus, while the unique profile of decidual macrophages is important in fetal protection, an alteration of this gene expression could be, in part, involved in complications of pregnancy. Indeed, a number of genes regulated in this array were previously found to be associated with pregnancy complications such as preeclampsia (MR , CCL2 , IGF-1 , secreted phosphoprotein (SPP)-1 , MMP-9 ), preterm labour (CCL18 ) and intrauterine growth restriction (IGF-1 ).
Purity of cell populations is extremely important in gene expression profiling. Our strategy of using gene expression in the highly purified samples as a requirement for further consideration resulted in high-stringent criteria minimizing the risk of reporting contaminating genes. The distinction between macrophages and dendritic cells in the decidua is not clear-cut. Dendritic cells may express the macrophage related CD14  whereas macrophages may express the DC related CD209. , . On the other hand, CD14+ decidual cells were shown to express HLA-DR, CD68 and CD209, but were negative in CD1a, CD83 and CD86  and, in addition, CD14+ cells were not able to differentiate into DCs when cultured in GM-CSF and IL-4 , thus indicating that CD14+ decidual cells mainly resemble macrophages. In any case, the expression profile of the major population of CD14 cells is of relevance irrespective of its relation to dendritic cells.
In conclusion, we present here the global gene expression pattern of decidual CD14 positive macrophages. The up-regulation of cell cycle genes indicates a highly proliferative nature of decidual cells. Although a number of nuclear factors were regulated, compatible with the differentiation process of decidua infiltrating macrophages, the main block of the regulated genes represented membrane receptors or secreted proteins, pointing to a profound polarization. The expression profile, with a large number of regulated genes related to immunomodulation and tissue remodelling, mainly parallels that of M2 polarized macrophages, including M2 markers such as CCL18, CD209, IGF-1, MRC-1 and fibronectin-1. Further, the up-regulation of for example TREM2, A2M and PGDS provide new insights into the regulating function of macrophages in pregnancy, and several genes might also be implicated in immune tolerance in general. Interestingly, some of the regulated genes described here for normal pregnancies, have been shown to be dysregulated in complicated pregnancies. Taken together, the mapping of decidual CD14 positive macrophages showed a unique transcriptional profile that confirms and extends previous knowledge about these cells as important components of fetal protection.
We thank the Department of Obstetrics and Gynecology for help with collecting samples for the study, Jan-Ingvar Jönsson and Florence Sjögren for invaluable advice in the FACSAria cell sorting and Karin Backteman for help with flow cytometry analyses. We also want to thank Tanja Töpfer for performing the array analyses, Kiki Dienus for real-time PCR, Martina Sandberg and Maria Jenmalm for ELISA analyses, Petra Cassel for Luminex analyses and Olle Ståhl for helpful input on the gene data.
Conceived and designed the experiments: CG JM AM LM GB JE. Performed the experiments: RG CG JM AM. Analyzed the data: RG SS CG JM AM JE. Contributed reagents/materials/analysis tools: RG JB CG JM AM LM GB JE. Wrote the paper: CG. Other: Revision of manuscript: LM GB SS JB RG. Drafting of the manuscript: JE AM JM.
- 1. Koopman LA, Kopcow HD, Rybalov B, Boyson JE, Orange JS, et al. (2003) Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential. J Exp Med 198: 1201–1212.LA KoopmanHD KopcowB. RybalovJE BoysonJS Orange2003Human decidual natural killer cells are a unique NK cell subset with immunomodulatory potential.J Exp Med19812011212
- 2. Stein M, Keshav S, Harris N, Gordon S (1992) Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation. J Exp Med 176: 287–292.M. SteinS. KeshavN. HarrisS. Gordon1992Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation.J Exp Med176287292
- 3. Gordon S (2003) Alternative activation of macrophages. Nat Rev Immunol 3: 23–35.S. Gordon2003Alternative activation of macrophages.Nat Rev Immunol32335
- 4. Goerdt S, Orfanos CE (1999) Other functions, other genes: alternative activation of antigen-presenting cells. Immunity 10: 137–142.S. GoerdtCE Orfanos1999Other functions, other genes: alternative activation of antigen-presenting cells.Immunity10137142
- 5. Mantovani A, Sica A, Sozzani S, Allavena P, Vecchi A, et al. (2004) The chemokine system in diverse forms of macrophage activation and polarization. Trends Immunol 25: 677–686.A. MantovaniA. SicaS. SozzaniP. AllavenaA. Vecchi2004The chemokine system in diverse forms of macrophage activation and polarization.Trends Immunol25677686
- 6. Mor G, Abrahams VM (2003) Potential role of macrophages as immunoregulators of pregnancy. Reprod Biol Endocrinol 1: 119.G. MorVM Abrahams2003Potential role of macrophages as immunoregulators of pregnancy.Reprod Biol Endocrinol1119
- 7. Chaouat G, Cayol V, Mairovitz V, Dubanchet S (1999) Localization of the Th2 cytokines IL-3, IL-4, IL-10 at the fetomaternal interface during human and murine pregnancy and lack of requirement for Fas/Fas ligand interaction for a successful allogeneic pregnancy. Am J Reprod Immunol 42: 1–13.G. ChaouatV. CayolV. MairovitzS. Dubanchet1999Localization of the Th2 cytokines IL-3, IL-4, IL-10 at the fetomaternal interface during human and murine pregnancy and lack of requirement for Fas/Fas ligand interaction for a successful allogeneic pregnancy.Am J Reprod Immunol42113
- 8. Sacks GP, Clover LM, Bainbridge DR, Redman CW, Sargent IL (2001) Flow cytometric measurement of intracellular Th1 and Th2 cytokine production by human villous and extravillous cytotrophoblast. Placenta 22: 550–559.GP SacksLM CloverDR BainbridgeCW RedmanIL Sargent2001Flow cytometric measurement of intracellular Th1 and Th2 cytokine production by human villous and extravillous cytotrophoblast.Placenta22550559
- 9. Lidström C, Matthiesen L, Berg G, Sharma S, Ernerudh J, et al. (2003) Cytokine secretion patterns of NK cells and macrophages in early human pregnancy decidua and blood: implications for suppressor macrophages in decidua. Am J Reprod Immunol 50: 444–452.C. LidströmL. MatthiesenG. BergS. SharmaJ. Ernerudh2003Cytokine secretion patterns of NK cells and macrophages in early human pregnancy decidua and blood: implications for suppressor macrophages in decidua.Am J Reprod Immunol50444452
- 10. Gustafsson C, Hummerdal P, Matthiesen L, Berg G, Ekerfelt C, et al. (2006) Cytokine secretion in decidual mononuclear cells from term human pregnancy with or without labour: ELISPOT detection of IFN-gamma, IL-4, IL-10, TGF-beta and TNF-alpha. J Reprod Immunol 71: 41–56.C. GustafssonP. HummerdalL. MatthiesenG. BergC. Ekerfelt2006Cytokine secretion in decidual mononuclear cells from term human pregnancy with or without labour: ELISPOT detection of IFN-gamma, IL-4, IL-10, TGF-beta and TNF-alpha.J Reprod Immunol714156
- 11. Dealtry GB, Clark DE, Sharkey A, Charnock-Jones DS, Smith SK (1998) Expression and localization of the Th2-type cytokine interleukin-13 and its receptor in the placenta during human pregnancy. Am J Reprod Immunol 40: 283–290.GB DealtryDE ClarkA. SharkeyDS Charnock-JonesSK Smith1998Expression and localization of the Th2-type cytokine interleukin-13 and its receptor in the placenta during human pregnancy.Am J Reprod Immunol40283290
- 12. Rieger L, Hofmeister V, Probe C, Dietl J, Weiss EH, et al. (2002) Th1- and Th2-like cytokine production by first trimester decidual large granular lymphocytes is influenced by HLA-G and HLA-E. Mol Hum Reprod 8: 255–261.L. RiegerV. HofmeisterC. ProbeJ. DietlEH Weiss2002Th1- and Th2-like cytokine production by first trimester decidual large granular lymphocytes is influenced by HLA-G and HLA-E.Mol Hum Reprod8255261
- 13. Brown M, Gustafson M, Saldana S, Baradaran A, Miller H, et al. (2004) Correlation of human decidual and cord blood mononuclear cell cytokine production. Hum Immunol 65: 1336–1343.M. BrownM. GustafsonS. SaldanaA. BaradaranH. Miller2004Correlation of human decidual and cord blood mononuclear cell cytokine production.Hum Immunol6513361343
- 14. Roth I, Corry DB, Locksley RM, Abrams JS, Litton MJ, et al. (1996) Human placental cytotrophoblasts produce the immunosuppressive cytokine interleukin 10. J Exp Med 184: 539–548.I. RothDB CorryRM LocksleyJS AbramsMJ Litton1996Human placental cytotrophoblasts produce the immunosuppressive cytokine interleukin 10.J Exp Med184539548
- 15. Hanna N, Hanna I, Hleb M, Wagner E, Dougherty J, et al. (2000) Gestational age-dependent expression of IL-10 and its receptor in human placental tissues and isolated cytotrophoblasts. J Immunol 164: 5721–5728.N. HannaI. HannaM. HlebE. WagnerJ. Dougherty2000Gestational age-dependent expression of IL-10 and its receptor in human placental tissues and isolated cytotrophoblasts.J Immunol16457215728
- 16. Mues B, Langer D, Zwadlo G, Sorg C (1989) Phenotypic characterization of macrophages in human term placenta. Immunology 67: 303–307.B. MuesD. LangerG. ZwadloC. Sorg1989Phenotypic characterization of macrophages in human term placenta.Immunology67303307
- 17. Hunt JS, Pollard JW (1992) Macrophages in the uterus and placenta. Curr Top Microbiol Immunol 181: 39–63.JS HuntJW Pollard1992Macrophages in the uterus and placenta.Curr Top Microbiol Immunol1813963
- 18. Cupurdija K, Azzola D, Hainz U, Gratchev A, Heitger A, et al. (2004) Macrophages of Human First Trimester Decidua Express Markers Associated to Alternative Activation. Am J Reprod Immunol 51: 117–122.K. CupurdijaD. AzzolaU. HainzA. GratchevA. Heitger2004Macrophages of Human First Trimester Decidua Express Markers Associated to Alternative Activation.Am J Reprod Immunol51117122
- 19. Heikkinen J, Mottonen M, Komi J, Alanen A, Lassila O (2003) Phenotypic characterization of human decidual macrophages. Clin Exp Immunol 131: 498–505.J. HeikkinenM. MottonenJ. KomiA. AlanenO. Lassila2003Phenotypic characterization of human decidual macrophages.Clin Exp Immunol131498505
- 20. Parhar RS, Kennedy TG, Lala PK (1988) Suppression of lymphocyte alloreactivity by early gestational human decidua. I. Characterization of suppressor cells and suppressor molecules. Cell Immunol 116: 392–410.RS ParharTG KennedyPK Lala1988Suppression of lymphocyte alloreactivity by early gestational human decidua. I. Characterization of suppressor cells and suppressor molecules.Cell Immunol116392410
- 21. Mizuno M, Aoki K, Kimbara T (1994) Functions of macrophages in human decidual tissue in early pregnancy. Am J Reprod Immunol 31: 180–188.M. MizunoK. AokiT. Kimbara1994Functions of macrophages in human decidual tissue in early pregnancy.Am J Reprod Immunol31180188
- 22. Martinez FO, Gordon S, Locati M, Mantovani A (2006) Transcriptional profiling of the human monocyte-to-macrophage differentiation and polarization: new molecules and patterns of gene expression. J Immunol 177: 7303–7311.FO MartinezS. GordonM. LocatiA. Mantovani2006Transcriptional profiling of the human monocyte-to-macrophage differentiation and polarization: new molecules and patterns of gene expression.J Immunol17773037311
- 23. Hashimoto S, Morohoshi K, Suzuki T, Matsushima K (2003) Lipopolysaccharide-inducible gene expression profile in human monocytes. Scand J Infect Dis 35: 619–627.S. HashimotoK. MorohoshiT. SuzukiK. Matsushima2003Lipopolysaccharide-inducible gene expression profile in human monocytes.Scand J Infect Dis35619627
- 24. Volpe E, Cappelli G, Grassi M, Martino A, Serafino A, et al. (2006) Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. Immunology 118: 449–460.E. VolpeG. CappelliM. GrassiA. MartinoA. Serafino2006Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis.Immunology118449460
- 25. Kodelja V, Muller C, Politz O, Hakij N, Orfanos CE, et al. (1998) Alternative macrophage activation-associated CC-chemokine-1, a novel structural homologue of macrophage inflammatory protein-1 alpha with a Th2-associated expression pattern. J Immunol 160: 1411–1418.V. KodeljaC. MullerO. PolitzN. HakijCE Orfanos1998Alternative macrophage activation-associated CC-chemokine-1, a novel structural homologue of macrophage inflammatory protein-1 alpha with a Th2-associated expression pattern.J Immunol16014111418
- 26. Puig-Kröger A, Serrano-Gomez D, Caparros E, Dominguez-Soto A, Relloso M, et al. (2004) Regulated expression of the pathogen receptor dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin in THP-1 human leukemic cells, monocytes, and macrophages. J Biol Chem 279: 25680–25688.A. Puig-KrögerD. Serrano-GomezE. CaparrosA. Dominguez-SotoM. Relloso2004Regulated expression of the pathogen receptor dendritic cell-specific intercellular adhesion molecule 3 (ICAM-3)-grabbing nonintegrin in THP-1 human leukemic cells, monocytes, and macrophages.J Biol Chem2792568025688
- 27. Laskarin G, Cupurdija K, Tokmadzic VS, Dorcic D, Dupor J, et al. (2005) The presence of functional mannose receptor on macrophages at the maternal-fetal interface. Hum Reprod 20: 1057–1066.G. LaskarinK. CupurdijaVS TokmadzicD. DorcicJ. Dupor2005The presence of functional mannose receptor on macrophages at the maternal-fetal interface.Hum Reprod2010571066
- 28. Burk MR, Troeger C, Brinkhaus R, Holzgreve W, Hahn S (2001) Severely reduced presence of tissue macrophages in the basal plate of pre-eclamptic placentae. Placenta 22: 309–316.MR BurkC. TroegerR. BrinkhausW. HolzgreveS. Hahn2001Severely reduced presence of tissue macrophages in the basal plate of pre-eclamptic placentae.Placenta22309316
- 29. Soilleux EJ, Morris LS, Leslie G, Chehimi J, Luo Q, et al. (2002) Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro. J Leukoc Biol 71: 445–457.EJ SoilleuxLS MorrisG. LeslieJ. ChehimiQ. Luo2002Constitutive and induced expression of DC-SIGN on dendritic cell and macrophage subpopulations in situ and in vitro.J Leukoc Biol71445457
- 30. Kämmerer U, Eggert AO, Kapp M, McLellan AD, Geijtenbeek TB, et al. (2003) Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy. Am J Pathol 162: 887–896.U. KämmererAO EggertM. KappAD McLellanTB Geijtenbeek2003Unique appearance of proliferating antigen-presenting cells expressing DC-SIGN (CD209) in the decidua of early human pregnancy.Am J Pathol162887896
- 31. Clark DA, Keil A, Chen Z, Markert U, Manuel J, et al. (2003) Placental trophoblast from successful human pregnancies expresses the tolerance signaling molecule, CD200 (OX-2). Am J Reprod Immunol 50: 187–195.DA ClarkA. KeilZ. ChenU. MarkertJ. Manuel2003Placental trophoblast from successful human pregnancies expresses the tolerance signaling molecule, CD200 (OX-2).Am J Reprod Immunol50187195
- 32. Bruder D, Probst-Kepper M, Westendorf AM, Geffers R, Beissert S, et al. (2004) Neuropilin-1: a surface marker of regulatory T cells. Eur J Immunol 34: 623–630.D. BruderM. Probst-KepperAM WestendorfR. GeffersS. Beissert2004Neuropilin-1: a surface marker of regulatory T cells.Eur J Immunol34623630
- 33. Turnbull IR, Gilfillan S, Cella M, Aoshi T, Miller M, et al. (2006) Cutting edge: TREM-2 attenuates macrophage activation. J Immunol 177: 3520–3524.IR TurnbullS. GilfillanM. CellaT. AoshiM. Miller2006Cutting edge: TREM-2 attenuates macrophage activation.J Immunol17735203524
- 34. Begum NA, Ishii K, Kurita-Taniguchi M, Tanabe M, Kobayashi M, et al. (2004) Mycobacterium bovis BCG cell wall-specific differentially expressed genes identified by differential display and cDNA subtraction in human macrophages. Infect Immun 72: 937–948.NA BegumK. IshiiM. Kurita-TaniguchiM. TanabeM. Kobayashi2004Mycobacterium bovis BCG cell wall-specific differentially expressed genes identified by differential display and cDNA subtraction in human macrophages.Infect Immun72937948
- 35. Hamerman JA, Jarjoura JR, Humphrey MB, Nakamura MC, Seaman WE, et al. (2006) Cutting edge: inhibition of TLR and FcR responses in macrophages by triggering receptor expressed on myeloid cells (TREM)-2 and DAP12. J Immunol 177: 2051–2055.JA HamermanJR JarjouraMB HumphreyMC NakamuraWE Seaman2006Cutting edge: inhibition of TLR and FcR responses in macrophages by triggering receptor expressed on myeloid cells (TREM)-2 and DAP12.J Immunol17720512055
- 36. Takahashi K, Rochford CD, Neumann H (2005) Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2. J Exp Med 201: 647–657.K. TakahashiCD RochfordH. Neumann2005Clearance of apoptotic neurons without inflammation by microglial triggering receptor expressed on myeloid cells-2.J Exp Med201647657
- 37. van Lieshout AW, van der Voort R, le Blanc LM, Roelofs MF, Schreurs BW, et al. (2006) Novel insights in the regulation of CCL18 secretion by monocytes and dendritic cells via cytokines, toll-like receptors and rheumatoid synovial fluid. BMC Immunol 7: 23.AW van LieshoutR. van der VoortLM le BlancMF RoelofsBW Schreurs2006Novel insights in the regulation of CCL18 secretion by monocytes and dendritic cells via cytokines, toll-like receptors and rheumatoid synovial fluid.BMC Immunol723
- 38. Marvin KW, Keelan JA, Eykholt RL, Sato TA, Mitchell MD (2002) Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm. Mol Hum Reprod 8: 399–408.KW MarvinJA KeelanRL EykholtTA SatoMD Mitchell2002Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm.Mol Hum Reprod8399408
- 39. Daly C, Rollins BJ (2003) Monocyte chemoattractant protein-1 (CCL2) in inflammatory disease and adaptive immunity: therapeutic opportunities and controversies. Microcirculation 10: 247–257.C. DalyBJ Rollins2003Monocyte chemoattractant protein-1 (CCL2) in inflammatory disease and adaptive immunity: therapeutic opportunities and controversies.Microcirculation10247257
- 40. Ip WK, Wong CK, Lam CW (2006) Interleukin (IL)-4 and IL-13 up-regulate monocyte chemoattractant protein-1 expression in human bronchial epithelial cells: involvement of p38 mitogen-activated protein kinase, extracellular signal-regulated kinase 1/2 and Janus kinase-2 but not c-Jun NH2-terminal kinase 1/2 signalling pathways. Clin Exp Immunol 145: 162–172.WK IpCK WongCW Lam2006Interleukin (IL)-4 and IL-13 up-regulate monocyte chemoattractant protein-1 expression in human bronchial epithelial cells: involvement of p38 mitogen-activated protein kinase, extracellular signal-regulated kinase 1/2 and Janus kinase-2 but not c-Jun NH2-terminal kinase 1/2 signalling pathways.Clin Exp Immunol145162172
- 41. Saito S, Tsuda H, Michimata T (2002) Prostaglandin D2 and reproduction. Am J Reprod Immunol 47: 295–302.S. SaitoH. TsudaT. Michimata2002Prostaglandin D2 and reproduction.Am J Reprod Immunol47295302
- 42. Munn DH, Zhou M, Attwood JT, Bondarev I, Conway SJ, et al. (1998) Prevention of allogeneic fetal rejection by tryptophan catabolism. Science 281: 1191–1193.DH MunnM. ZhouJT AttwoodI. BondarevSJ Conway1998Prevention of allogeneic fetal rejection by tryptophan catabolism.Science28111911193
- 43. Terness P, Kallikourdis M, Betz AG, Rabinovich GA, Saito S, et al. (2007) Tolerance signaling molecules and pregnancy: IDO, galectins, and the renaissance of regulatory T cells. Am J Reprod Immunol 58: 238–254.P. TernessM. KallikourdisAG BetzGA RabinovichS. Saito2007Tolerance signaling molecules and pregnancy: IDO, galectins, and the renaissance of regulatory T cells.Am J Reprod Immunol58238254
- 44. Tayade C, Esadeg S, Fang Y, Croy BA (2005) Functions of alpha 2 macroglobulins in pregnancy. Mol Cell Endocrinol 245: 60–66.C. TayadeS. EsadegY. FangBA Croy2005Functions of alpha 2 macroglobulins in pregnancy.Mol Cell Endocrinol2456066
- 45. Larin SS, Gorlina NK, Kozlov IG, Cheredeev AN, Zorin NA, et al. (2002) Binding of alpha2-macroglobulin to collagen type I: modification of collagen matrix by alpha2-macroglobulin induces the enhancement of macrophage migration. Russ J Immunol 7: 34–40.SS LarinNK GorlinaIG KozlovAN CheredeevNA Zorin2002Binding of alpha2-macroglobulin to collagen type I: modification of collagen matrix by alpha2-macroglobulin induces the enhancement of macrophage migration.Russ J Immunol73440
- 46. Nayak NR, Giudice LC (2003) Comparative biology of the IGF system in endometrium, decidua, and placenta, and clinical implications for foetal growth and implantation disorders. Placenta 24: 281–296.NR NayakLC Giudice2003Comparative biology of the IGF system in endometrium, decidua, and placenta, and clinical implications for foetal growth and implantation disorders.Placenta24281296
- 47. Kodelja V, Muller C, Tenorio S, Schebesch C, Orfanos CE, et al. (1997) Differences in angiogenic potential of classically vs alternatively activated macrophages. Immunobiology 197: 478–493.V. KodeljaC. MullerS. TenorioC. SchebeschCE Orfanos1997Differences in angiogenic potential of classically vs alternatively activated macrophages.Immunobiology197478493
- 48. Kämmerer U (2005) Antigen-presenting cells in the decidua. Chem Immunol Allergy 89: 96–104.U. Kämmerer2005Antigen-presenting cells in the decidua.Chem Immunol Allergy8996104
- 49. Cheung DL, Hamilton JA (1992) Regulation of human monocyte DNA synthesis by colony-stimulating factors, cytokines, and cyclic adenosine monophosphate. Blood 79: 1972–1981.DL CheungJA Hamilton1992Regulation of human monocyte DNA synthesis by colony-stimulating factors, cytokines, and cyclic adenosine monophosphate.Blood7919721981
- 50. Bischof RJ, Zafiropoulos D, Hamilton JA, Campbell IK (2000) Exacerbation of acute inflammatory arthritis by the colony-stimulating factors CSF-1 and granulocyte macrophage (GM)-CSF: evidence of macrophage infiltration and local proliferation. Clin Exp Immunol 119: 361–367.RJ BischofD. ZafiropoulosJA HamiltonIK Campbell2000Exacerbation of acute inflammatory arthritis by the colony-stimulating factors CSF-1 and granulocyte macrophage (GM)-CSF: evidence of macrophage infiltration and local proliferation.Clin Exp Immunol119361367
- 51. Stout RD, Suttles J (2004) Functional plasticity of macrophages: reversible adaptation to changing microenvironments. J Leukoc Biol 76: 509–513.RD StoutJ. Suttles2004Functional plasticity of macrophages: reversible adaptation to changing microenvironments.J Leukoc Biol76509513
- 52. Takabayshi K, Corr M, Hayashi T, Redecke V, Beck L, et al. (2006) Induction of a homeostatic circuit in lung tissue by microbial compounds. Immunity 24: 475–487.K. TakabayshiM. CorrT. HayashiV. RedeckeL. Beck2006Induction of a homeostatic circuit in lung tissue by microbial compounds.Immunity24475487
- 53. Lewis CE, Pollard JW (2006) Distinct role of macrophages in different tumor microenvironments. Cancer Res 66: 605–612.CE LewisJW Pollard2006Distinct role of macrophages in different tumor microenvironments.Cancer Res66605612
- 54. Rutella S, Bonanno G, Procoli A, Mariotti A, de Ritis DG, et al. (2006) Hepatocyte growth factor favors monocyte differentiation into regulatory interleukin (IL)-10++IL-12low/neg accessory cells with dendritic-cell features. Blood 108: 218–227.S. RutellaG. BonannoA. ProcoliA. MariottiDG de Ritis2006Hepatocyte growth factor favors monocyte differentiation into regulatory interleukin (IL)-10++IL-12low/neg accessory cells with dendritic-cell features.Blood108218227
- 55. Aoki S, Hata T, Manabe A, Miyazaki K (1998) Decreased maternal circulating hepatocyte growth factor (HGF) concentrations in pregnancies with small for gestational age infants. Hum Reprod 13: 2950–2953.S. AokiT. HataA. ManabeK. Miyazaki1998Decreased maternal circulating hepatocyte growth factor (HGF) concentrations in pregnancies with small for gestational age infants.Hum Reprod1329502953
- 56. Baykal C, Guler G, Al A, Tulunay G, Ozer S, et al. (2005) Expression of hepatocyte growth factor/scatter factor receptor in IUGR fetuses' placentas: an immunohistochemical analysis. Fetal Diagn Ther 20: 249–253.C. BaykalG. GulerA. AlG. TulunayS. Ozer2005Expression of hepatocyte growth factor/scatter factor receptor in IUGR fetuses' placentas: an immunohistochemical analysis.Fetal Diagn Ther20249253
- 57. Furugori K, Kurauchi O, Itakura A, Kanou Y, Murata Y, et al. (1997) Levels of hepatocyte growth factor and its messenger ribonucleic acid in uncomplicated pregnancies and those complicated by preeclampsia. J Clin Endocrinol Metab 82: 2726–2730.K. FurugoriO. KurauchiA. ItakuraY. KanouY. Murata1997Levels of hepatocyte growth factor and its messenger ribonucleic acid in uncomplicated pregnancies and those complicated by preeclampsia.J Clin Endocrinol Metab8227262730
- 58. Watkins SK, Egilmez NK, Suttles J, Stout RD (2007) IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo. J Immunol 178: 1357–1362.SK WatkinsNK EgilmezJ. SuttlesRD Stout2007IL-12 rapidly alters the functional profile of tumor-associated and tumor-infiltrating macrophages in vitro and in vivo.J Immunol17813571362
- 59. Gabinskaya T, Salafia CM, Gulle VE, Holzman IR, Weintraub AS (1998) Gestational age-dependent extravillous cytotrophoblast osteopontin immunolocalization differentiates between normal and preeclamptic pregnancies. Am J Reprod Immunol 40: 339–346.T. GabinskayaCM SalafiaVE GulleIR HolzmanAS Weintraub1998Gestational age-dependent extravillous cytotrophoblast osteopontin immunolocalization differentiates between normal and preeclamptic pregnancies.Am J Reprod Immunol40339346
- 60. Coolman M, de Maat M, Van Heerde WL, Felida L, Schoormans S, et al. (2007) Matrix Metalloproteinase-9 Gene -1562C/T Polymorphism Mitigates Preeclampsia. Placenta 28: 709–713.M. CoolmanM. de MaatWL Van HeerdeL. FelidaS. Schoormans2007Matrix Metalloproteinase-9 Gene -1562C/T Polymorphism Mitigates Preeclampsia.Placenta28709713