Mild cold exposure and overfeeding are known to elevate energy expenditure in mammals, including humans. This process is called adaptive thermogenesis. In small animals, adaptive thermogenesis is mainly caused by mitochondrial uncoupling in brown adipose tissue and regulated via the sympathetic nervous system. In humans, skeletal muscle is a candidate tissue, known to account for a large part of the epinephrine-induced increase in energy expenditure. However, mitochondrial uncoupling in skeletal muscle has not extensively been studied in relation to adaptive thermogenesis in humans. Therefore we hypothesized that cold-induced adaptive thermogenesis in humans is accompanied by an increase in mitochondrial uncoupling in skeletal muscle.
The metabolic response to mild cold exposure in 11 lean, male subjects was measured in a respiration chamber at baseline and mild cold exposure. Skeletal muscle mitochondrial uncoupling (state 4) was measured in muscle biopsies taken at the end of the respiration chamber stays. Mild cold exposure caused a significant increase in 24h energy expenditure of 2.8% (0.32 MJ/day, range of −0.21 to 1.66 MJ/day, p<0.05). The individual increases in energy expenditure correlated to state 4 respiration (p<0.02, R2 = 0.50).
This study for the first time shows that in humans, skeletal muscle has the intrinsic capacity for cold induced adaptive thermogenesis via mitochondrial uncoupling under physiological conditions. This opens possibilities for mitochondrial uncoupling as an alternative therapeutic target in the treatment of obesity.
Citation: Wijers SLJ, Schrauwen P, Saris WHM, van Marken Lichtenbelt WD (2008) Human Skeletal Muscle Mitochondrial Uncoupling Is Associated with Cold Induced Adaptive Thermogenesis. PLoS ONE 3(3): e1777. https://doi.org/10.1371/journal.pone.0001777
Academic Editor: Alessandro Bartolomucci, University of Parma, Italy
Received: July 30, 2007; Accepted: February 11, 2008; Published: March 12, 2008
Copyright: © 2008 Wijers et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
Obesity is reaching epidemic forms, and is expected to have a major impact on public health in the Western world –. It develops when energy intake exceeds energy expenditure for a prolonged period of time. Despite the strategies aimed at reducing energy intake, the prevalence of obesity is still rising. Therefore, augmenting energy expenditure is an attractive alternative strategy to tackle the obesity epidemic. Mild cold exposure is known to elevate energy expenditure in mammals, including humans. This regulated increase in energy expenditure is called adaptive thermogenesis .
In small mammals brown adipose tissue is responsible for the major part of adaptive thermogenesis via the action of the brown-adipose tissue specific uncoupling protein 1 (UCP-1), controlled by the sympathetic nervous system . This protein is able to lower the proton gradient across the mitochondrial inner membrane and bypasses ATP-synthase (i.e. mitochondrial uncoupling), thereby preventing ATP production and dissipating energy as heat .
Although the impact of brown adipose tissue in adult humans is questionable and still under debate , , evidence can be obtained for the existence of adaptive thermogenesis. For example, it has been shown that a fixed excess of calories by overeating causes a three-fold range in weight gain among subjects . Also after mild cold exposure a large range in the increase in energy expenditure has been found . In humans, it is shown that skeletal muscle can be responsible for a 40% increase in total body energy expenditure after epinephrine infusion . However, no data on human skeletal muscle mitochondrial uncoupling in relation to adaptive thermogenesis are available as yet.
Here we hypothesize that skeletal muscle has the intrinsic capacity for adaptive thermogenesis in humans via mitochondrial uncoupling. We investigated energy expenditure, mitochondrial uncoupling, and expression of selected proteins after cold exposure in eleven healthy male subjects.
Eleven lean male subjects stayed twice in a respiration chamber: once for a baseline measurement (energy balance, 22°C) and once during mild cold exposure (energy balance, 16°C). After each stay a muscle biopsy was taken.
Total daily energy expenditure (TDEE) was measured for the last 24 hours of each stay. TDEE increased significantly with 2.8% (0.32 MJ/day, z = 1.96, p<0.05) (figure 1a). The inter-subject variability in the change in TDEE was large (range of −0.21 to 1.66 MJ/day). The increase in TDEE was not explained by increased physical activity: in fact mild cold exposure resulted in a significant decrease in physical activity (−39 kcounts/day, z = −2.67, p<0.01) (figure 1b).
The figures show mean (±SEM) in both situations. * p<0.05, ** p<0.01
Human skeletal muscle mitochondrial uncoupling was assessed using high-resolution respirometry (see methods). Oxygen consumption was measured in permeabilized muscle fibers. Addition of mitochondrial substrates and ADP leads to a basal level of respiration, called state 3. After addition of oligomycin, ATP-synthase is blocked and the remaining oxygen consumption can only be due to proton leakage. This remaining respiration is called state 4, and is a measure of mitochondrial uncoupling. Mitochondrial respiration measurements have been corrected for citrate synthase activity, as a measure of mitochondrial content. Citrate synthase activity did not differ on average between baseline and cold exposure measurements. On average, neither state 4 nor state 3 did increase after mild cold exposure (respectively z = −1.38, p = 0.168 and z = −1.38, p = 0.168) (figure 1c and 1d). However, interindividual differences were large (state 4: range of −0.12 to 0.17 pmol O2/(s·mg muscle·CS activity); state 3: range of −0.22 to 0.62 pmol O2/(s·mg muscle·CS activity)). Changes in TDEE correlated significantly to state 4 respiration (p = 0.015, R2 = 0.50) (figure 2). No correlation of TDEE with state 3 was observed.
Since UCP-3 content and mitochondrial density can change muscular energy expenditure, UCP-3 and oxidative phosphorylation protein content and citrate synthase activity have been measured in a separate set of 9 subjects under identical experimental conditions. UCP-3 and oxidative phosphorylation protein complex I, II, III, IV, and V expression did not change significantly (table 1).
The results show that human adaptive thermogenesis in response to cold exposure is accompanied by and related to mitochondrial uncoupling in skeletal muscle. Fifty percent of the variation in the individual increases in energy expenditure upon cold exposure can be explained by mitochondrial uncoupling
Since mitochondrial protein expression and citrate synthase activity did not increase after mild cold exposure, an augmentation of mitochondrial density can be ruled out . Also an increase in physical activity can be excluded, since radar counts were lower–and not higher–after cold exposure. Shivering can be excluded too; subjects reported they did not perceive any shivering at all. Furthermore, in a previous study in the same study setting, this has been validated by electromyogram measurements .
The process of skeletal muscle mitochondria uncoupling is thus likely to contribute to the increase in energy expenditure. Previously, it has been shown that mitochondrial uncoupling in humans could be stimulated. Infusion of T3, which is a known factor involved in cellular respiration, resulted in higher mitochondrial uncoupling . However, this study for the first time shows that environmental factors affect both adaptive thermogenesis and mitochondrial uncoupling.
The increase in energy expenditure, 0.32 MJ/day (−0.20 to 1.66 MJ/day), is comparable to data from literature , , . This increase is considerably smaller than the increase in energy expenditure observed in small mammals after cold exposure (two- to fourfold) . However, a small difference in energy expenditure for a prolonged period might contribute largely to weight gain. A diminished adaptive thermogenesis has indeed been identified as a risk factor for obesity . Therefore, our results indicate that mitochondrial uncoupling in human skeletal muscle may be a target for therapies to prevent obesity.
In the past, drugs have been developed to treat obesity, for example 2,4-Dinitrophenol, without insight in the mechanisms behind. This drug, which has a major effect on the uncoupling of oxidative phosphorylation, turned out to be lethal due to hyperthermia and has been banned in 1938, but is still used in illegal circuits due to its marked effects on body weight . The present study shows that skeletal muscle has the intrinsic capacity of regulated mitochondrial uncoupling. Research should aim to target this intrinsic uncoupling process in the mitochondrial membrane without disrupting the complete membrane as is the case with the use of 2,4-Dinitrophenol to increase thermogenesis and thus weight loss.
To gain more insight in the processes underlying cold-induced mitochondrial uncoupling, further research is needed to unravel the molecular nature of these processes in human skeletal muscle. Since UCP-1 and also the more recently discovered uncoupling proteins UCP-2, UCP-4, and UCP-5 are not present in human muscle –, other candidate actors have to be found. UCP-3 is present in human muscle, but it is currently believed to play a role in fatty acid metabolism rather than in direct mitochondrial uncoupling . This is underlined by our finding that UCP-3 protein expression was not upregulated during cold exposure. Therefore, so far unknown proteins may be found that are able to provoke mitochondrial uncoupling. Proteomics and genomics approaches would be methods of choice, since with these methods no prior targets have to be determined.
Acclimatization to cold is known to have large effects on adaptive thermogenesis. Subjects regularly exposed to mild cold have a higher adaptive thermogenesis capacity . This could be of benefit in situations of overfeeding, since cold-induced adaptive thermogenesis is related to overfeeding induced adaptive thermogenesis, as we have shown recently . Therefore, the results indicate that regular cold exposure might be beneficial in body weight regulation via an increase in the skeletal muscle uncoupling capacity.
In conclusion, human skeletal muscle has the intrinsic capacity for mitochondrial uncoupling. Cold-induced increases in energy expenditure are accompanied by an increase in skeletal muscle mitochondrial uncoupling.
Materials and Methods
Eleven lean male subjects (height: 184±3 cm; body mass: 77.7±2.7 kg; BMI: 22.9±0.6 kg/m2; age: 21.7±0.6 years), not using any medications and not seen by a MD in the last three years participated in this study. Subjects stayed twice in the respiration chambers of the department of Human Biology, Maastricht University, wearing standardized clothing. The two conditions are defined as follows: 1) baseline: 34 hours measurement of energy expenditure at energy balance, comfortable temperature (22°C); 2) cold exposure for 82 hours in energy balance, mild cold without shivering (16°C). Macro-nutrient composition was equal in both conditions: 47, 38, and 15% energy from carbohydrate, fat, and protein, respectively. After each respiration chamber stay a muscle biopsy was taken, from M. vastus lateralis according to the technique of Bergström . A part of the biopsy was stored in a preservation medium for mitochondrial uncoupling measurement, the rest of the biopsy was quickly frozen in liquid nitrogen for protein quantification and CS activity analyses.
The Medical Ethical Committee of Maastricht University/University Hospital Maastricht approved the protocol. All participants provided oral and written informed consent.
The respiration chambers are whole body calorimeters annex climate chambers . Total daily energy expenditure (TDEE) was determined from the subjects' O2 consumption, CO2 production and urine nitrogen excretion according to the Weir equation  during the last 24 hours of the respiration chamber stay. Physical activity was monitored during the last day of each stay using a radar system based on the Doppler principle, as described previously .
Mitochondrial respirometry measurements have been made in permeabilized muscle fibers. We have chosen for permeabilized fibers instead of isolated mitochondria since the mitochondria then remain in a more physiological environment.
Muscle tissue was prepared for mitochondrial respiration measurements as follows: after taking the muscle biopsy, the sample was stored in an ice-cold preservation buffer (10 mM Ca-EGTA buffer, 0.1 µM free calcium, 20 mM imidazole, 20 mM taurine, 50 mM K-MES, 0.5 mM DTT, 6.56 mM MgCl2, 5.77 mM ATP, 15 mM phosphocreatine, pH 7.1). Individual fibre bundles were separated using sharp needles to increase surface area. Subsequently fibers were permeabilized according to the method designed by Veksler et al  using saponin. After that, muscle fibers were washed in a respiration medium (0.5 mM EGTA, 3 mM MgCl2, 60 mM K-lactobionate, 20 mM Taurine, 10 mM KH2PO4, 20 mM HEPES, 110 mM Sucrose and 1 g/l BSA, pH 7.1).
Mitochondrial respiration measurements were carried out with 2–6 mg of permeabilized muscle fibre in the same respiration buffer in the Oxygraph-2K (Oroboros, Austria). Subsequently, malate (5 mM), ADP (2 mM), glutamate (10 mM), succinate (10 mM), and oligomycin (1 µg/ml) were added (figure 3). After succinate addition state 3 was achieved: the maximal oxygen consumption with production of ATP. After oligomycin addition, ATP-synthase was blocked and state 4 was measured: the oxygen consumption without ATP-production. Measurements have been normalized for the wet weight of added muscle tissue. Furthermore, respiration measurements have been corrected for citrate synthase activity (see below) to correct for mitochondrial content.
The blue line indicates the oxygen concentration in the sample. The red line indicates its derivative, the oxygen consumption by the sample in the respiration medium. The responses to the addition of malate (5 mM), ADP (2 mM), glutamate (10 mM), succinate (10 mM), and oligomycin (1 µg/ml) can be seen. After succinate addition, state 3 was achieved. After oligomycin addition, state 4 was achieved.
Protein quantification and CS activity
For UCP3 protein determination, muscle biopsies were homogenized in ice-cold Tris-EDTA buffer at pH 7.4, and then the homogenates were sonicated for 15 seconds. Subsequently, two volumes of each skeletal muscle homogenate and one volume of SDS-sample buffer were boiled for 4 min. Next, 13% polyacrylamide gels containing 0.1% SDS were loaded with equal amounts of protein from each sample, and electrophoresis was performed using a Mini-Protean 3 Electrophoresis Cell (Bio-Rad Laboratories, Hercules, CA). After gel electrophoreses, the gel was scanned, and the optical density of the 43-kDa band, previously immuno-identified to represent actin, was assessed. Then, a second gel was prepared and loaded with the sample volume (which had been recalculated based on the optical density of the actin band), after which Western blotting was performed using a Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad Laboratories) as described previously . We used a rabbit polyclonal UCP3 antibody (code 1331; kindly provided by LJ Slieker, Eli Lilly) prepared against a 20–amino acid (aa) peptide (human sequence aa 147–166). For a detailed description of the selectivity and specificity checks see previous reports , . Cytochrome C level, as a marker of mitochondrial content, was measured comparably using a rabbit polyclonal cytochrome c antibody (BD PharMingen, Woerden, NL). The ND6 subunit of complex I, the 30 kDa Ip subunit of complex II, the 47 kDa core protein 2 of complex III, subunit II of cytochrome C oxidase (complex IV) and the alpha subunit of the F1F0 ATP synthase (complex V) were measured using monoclonal antibodies (MitoSciences, Oregon, USA). All proteins were expressed as arbitrary units (AU). Citrate synthase activity has been measured following the method of Shephard and Garland .
Statistical analyses were done using SPSS 11 for Mac OS X. Data were reported as mean±SEM. Comparisons between groups were made using the non parametric Wilcoxon signed rank test. Correlation tests have been performed using Spearman correlation. A p-value smaller than 0.05 was considered to be statistically significant.
We would like to thank Paul Schoffelen and Loek Wouters for their assistance with the respiration chambers and Esther Moonen-Kornips for performing the western blotting and citrate synthase assays.
Conceived and designed the experiments: PS WS SW Wv. Performed the experiments: SW. Analyzed the data: SW. Contributed reagents/materials/analysis tools: PS. Wrote the paper: PS WS SW Wv.
- 1. World Health Organization (2000) Obesity: preventing and managing the global epidemic : report of a WHO consultation. Geneva: World Health Organization. World Health Organization2000Obesity: preventing and managing the global epidemic : report of a WHO consultation.GenevaWorld Health OrganizationXII, 253
- 2. Wyatt SB, Winters KP, Dubbert PM (2006) Overweight and obesity: prevalence, consequences, and causes of a growing public health problem. Am J Med Sci 331: 166–174.SB WyattKP WintersPM Dubbert2006Overweight and obesity: prevalence, consequences, and causes of a growing public health problem.Am J Med Sci331166174
- 3. Schokker DF, Visscher TL, Nooyens AC, van Baak MA, Seidell JC (2007) Prevalence of overweight and obesity in the Netherlands. Obes Rev 8: 101–107.DF SchokkerTL VisscherAC NooyensMA van BaakJC Seidell2007Prevalence of overweight and obesity in the Netherlands.Obes Rev8101107
- 4. Lowell BB, Spiegelman BM (2000) Towards a molecular understanding of adaptive thermogenesis. Nature 404: 652–660.BB LowellBM Spiegelman2000Towards a molecular understanding of adaptive thermogenesis.Nature404652660
- 5. Cannon B, Nedergaard J (2004) Brown adipose tissue: function and physiological significance. Physiol Rev 84: 277–359.B. CannonJ. Nedergaard2004Brown adipose tissue: function and physiological significance.Physiol Rev84277359
- 6. Himms-Hagen J (2001) Does Brown Adipose Tissue (BAT) Have a Role in the Physiology or Treatment of Human Obesity? Reviews in Endocrine & Metabolic Disorders 2: 395–401.J. Himms-Hagen2001Does Brown Adipose Tissue (BAT) Have a Role in the Physiology or Treatment of Human Obesity?Reviews in Endocrine & Metabolic Disorders2395401
- 7. Nedergaard J, Bengtsson T, Cannon B (2007) Unexpected evidence for active brown adipose tissue in adult humans. Am J Physiol Endocrinol Metab 293: E444–452.J. NedergaardT. BengtssonB. Cannon2007Unexpected evidence for active brown adipose tissue in adult humans.Am J Physiol Endocrinol Metab293E444452
- 8. Bouchard C, Tremblay A, Despres JP, Nadeau A, Lupien PJ, et al. (1990) The response to long-term overfeeding in identical twins. N Engl J Med 322: 1477–1482.C. BouchardA. TremblayJP DespresA. NadeauPJ Lupien1990The response to long-term overfeeding in identical twins.N Engl J Med32214771482
- 9. van Marken Lichtenbelt WD, Schrauwen P, van De Kerckhove S, Westerterp-Plantenga MS (2002) Individual variation in body temperature and energy expenditure in response to mild cold. Am J Physiol Endocrinol Metab 282: E1077–1083.WD van Marken LichtenbeltP. SchrauwenS. van De KerckhoveMS Westerterp-Plantenga2002Individual variation in body temperature and energy expenditure in response to mild cold.Am J Physiol Endocrinol Metab282E10771083
- 10. Simonsen L, Bulow J, Madsen J, Christensen NJ (1992) Thermogenic response to epinephrine in the forearm and abdominal subcutaneous adipose tissue. Am J Physiol 263: E850–855.L. SimonsenJ. BulowJ. MadsenNJ Christensen1992Thermogenic response to epinephrine in the forearm and abdominal subcutaneous adipose tissue.Am J Physiol263E850855
- 11. Schrauwen P, Mensink M, Schaart G, Moonen-Kornips E, Sels J-P, et al. (2006) Reduced Skeletal Muscle Uncoupling Protein-3 Content in Prediabetic Subjects and Type 2 Diabetic Patients: Restoration by Rosiglitazone Treatment. J Clin Endocrinol Metab 91: 1520–1525.P. SchrauwenM. MensinkG. SchaartE. Moonen-KornipsJ-P Sels2006Reduced Skeletal Muscle Uncoupling Protein-3 Content in Prediabetic Subjects and Type 2 Diabetic Patients: Restoration by Rosiglitazone Treatment.J Clin Endocrinol Metab9115201525
- 12. van Ooijen AM, van Marken Lichtenbelt WD, van Steenhoven AA, Westerterp KR (2004) Seasonal changes in metabolic and temperature responses to cold air in humans. Physiol Behav 82: 545–553.AM van OoijenWD van Marken LichtenbeltAA van SteenhovenKR Westerterp2004Seasonal changes in metabolic and temperature responses to cold air in humans.Physiol Behav82545553
- 13. Lebon V, Dufour S, Petersen KF, Ren J, Jucker BM, et al. (2001) Effect of triiodothyronine on mitochondrial energy coupling in human skeletal muscle. Journal of Clinical Investigation 108: 733–737.V. LebonS. DufourKF PetersenJ. RenBM Jucker2001Effect of triiodothyronine on mitochondrial energy coupling in human skeletal muscle.Journal of Clinical Investigation108733737
- 14. Warwick PM, Busby R (1990) Influence of mild cold on 24 h energy expenditure in ‘normally’ clothed adults. Br J Nutr 63: 481–488.PM WarwickR. Busby1990Influence of mild cold on 24 h energy expenditure in ‘normally’ clothed adults.Br J Nutr63481488
- 15. Harris AM, MacBride LR, Foster RC, McCrady SK, Levine JA (2006) Does non-exercise activity thermogenesis contribute to non-shivering thermogenesis? Journal of Thermal Biology 31: 634–638.AM HarrisLR MacBrideRC FosterSK McCradyJA Levine2006Does non-exercise activity thermogenesis contribute to non-shivering thermogenesis?Journal of Thermal Biology31634638
- 16. Davis TR, Johnston DR, Bell FC, Cremer BJ (1960) Regulation of shivering and non-shivering heat production during acclimation of rats. Am J Physiol 198: 471–475.TR DavisDR JohnstonFC BellBJ Cremer1960Regulation of shivering and non-shivering heat production during acclimation of rats.Am J Physiol198471475
- 17. Major GC, Doucet E, Trayhurn P, Astrup A, Tremblay A (2007) Clinical significance of adaptive thermogenesis. Int J Obes (Lond) 31: 204–212.GC MajorE. DoucetP. TrayhurnA. AstrupA. Tremblay2007Clinical significance of adaptive thermogenesis.Int J Obes (Lond)31204212
- 18. Miranda EJ, McIntyre IM, Parker DR, Gary RD, Logan BK (2006) Two deaths attributed to the use of 2,4-dinitrophenol. J Anal Toxicol 30: 219–222.EJ MirandaIM McIntyreDR ParkerRD GaryBK Logan2006Two deaths attributed to the use of 2,4-dinitrophenol.J Anal Toxicol30219222
- 19. Sanchis D, Fleury C, Chomiki N, Goubern M, Huang Q, et al. (1998) BMCP1, a novel mitochondrial carrier with high expression in the central nervous system of humans and rodents, and respiration uncoupling activity in recombinant yeast. J Biol Chem 273: 34611–34615.D. SanchisC. FleuryN. ChomikiM. GoubernQ. Huang1998BMCP1, a novel mitochondrial carrier with high expression in the central nervous system of humans and rodents, and respiration uncoupling activity in recombinant yeast.J Biol Chem2733461134615
- 20. Pecqueur C, Alves-Guerra MC, Gelly C, Levi-Meyrueis C, Couplan E, et al. (2001) Uncoupling protein 2, in vivo distribution, induction upon oxidative stress, and evidence for translational regulation. J Biol Chem 276: 8705–8712.C. PecqueurMC Alves-GuerraC. GellyC. Levi-MeyrueisE. Couplan2001Uncoupling protein 2, in vivo distribution, induction upon oxidative stress, and evidence for translational regulation.J Biol Chem27687058712
- 21. Mao W, Yu XX, Zhong A, Li W, Brush J, et al. (1999) UCP4, a novel brain-specific mitochondrial protein that reduces membrane potential in mammalian cells. FEBS Lett 443: 326–330.W. MaoXX YuA. ZhongW. LiJ. Brush1999UCP4, a novel brain-specific mitochondrial protein that reduces membrane potential in mammalian cells.FEBS Lett443326330
- 22. Schrauwen P, Hoeks J, Hesselink MK (2006) Putative function and physiological relevance of the mitochondrial uncoupling protein-3: involvement in fatty acid metabolism? Prog Lipid Res 45: 17–41.P. SchrauwenJ. HoeksMK Hesselink2006Putative function and physiological relevance of the mitochondrial uncoupling protein-3: involvement in fatty acid metabolism?Prog Lipid Res451741
- 23. Wijers SLJ, Saris WH, van Marken Lichtenbelt WD (2007) Individual thermogenic responses to mild cold and overfeeding are closely related. J Clin Endocrinol Metab 92: 4299–4305.SLJ WijersWH SarisWD van Marken Lichtenbelt2007Individual thermogenic responses to mild cold and overfeeding are closely related.J Clin Endocrinol Metab9242994305
- 24. Bergstrom J, Hermansen L, Hultman E, Saltin B (1967) Diet, muscle glycogen and physical performance. Acta Physiol Scand 71: 140–150.J. BergstromL. HermansenE. HultmanB. Saltin1967Diet, muscle glycogen and physical performance.Acta Physiol Scand71140150
- 25. Schoffelen PF, Westerterp KR, Saris WH, Ten Hoor F (1997) A dual-respiration chamber system with automated calibration. J Appl Physiol 83: 2064–2072.PF SchoffelenKR WesterterpWH SarisF. Ten Hoor1997A dual-respiration chamber system with automated calibration.J Appl Physiol8320642072
- 26. Weir JB (1949) New methods for calculating metabolic rate with special reference to protein metabolism. J Physiol 109: 1–9.JB Weir1949New methods for calculating metabolic rate with special reference to protein metabolism.J Physiol10919
- 27. Jequier E, Schutz Y (1983) Long-term measurements of energy expenditure in humans using a respiration chamber. Am J Clin Nutr 38: 989–998.E. JequierY. Schutz1983Long-term measurements of energy expenditure in humans using a respiration chamber.Am J Clin Nutr38989998
- 28. Veksler VI, Kuznetsov AV, Sharov VG, Kapelko VI, Saks VA (1987) Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers. Biochim Biophys Acta 892: 191–196.VI VekslerAV KuznetsovVG SharovVI KapelkoVA Saks1987Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers.Biochim Biophys Acta892191196
- 29. Hesselink MK, Greenhaff PL, Constantin-Teodosiu D, Hultman E, Saris WH, et al. (2003) Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo. J Clin Invest 111: 479–486.MK HesselinkPL GreenhaffD. Constantin-TeodosiuE. HultmanWH Saris2003Increased uncoupling protein 3 content does not affect mitochondrial function in human skeletal muscle in vivo.J Clin Invest111479486
- 30. Schrauwen P, Schaart G, Saris WHM, Slieker LJ, Glatz JFC, et al. (2000) The effect of weight reduction on skeletal muscle UCP2 and UCP3 mRNA expression and UCP3 protein content in Type II diabetic subjects. Diabetologia 43: 1408–1416.P. SchrauwenG. SchaartWHM SarisLJ SliekerJFC Glatz2000The effect of weight reduction on skeletal muscle UCP2 and UCP3 mRNA expression and UCP3 protein content in Type II diabetic subjects.Diabetologia4314081416
- 31. Shephard D, Garland PB (1969) Citrate synthase from rat liver. Methods Enzymol 13: 11–16.D. ShephardPB Garland1969Citrate synthase from rat liver.Methods Enzymol131116