Type II DNA topoisomerases (topos) are essential enzymes needed for the resolution of topological problems that occur during DNA metabolic processes. Topos carry out an ATP-dependent strand passage reaction whereby one double helix is passed through a transient break in another. Humans have two topoII isoforms, α and β, which while enzymatically similar are differentially expressed and regulated, and are thought to have different cellular roles. The C-terminal domain (CTD) of the enzyme has the most diversity, and has been implicated in regulation. We sought to investigate the impact of the CTD domain on activity.
We have investigated the role of the human topoII C-terminal domain by creating constructs encoding C-terminally truncated recombinant topoIIα and β and topoIIα+β-tail and topoIIβ+α-tail chimeric proteins. We then investigated function in vivo in a yeast system, and in vitro in activity assays. We find that the C-terminal domain of human topoII isoforms is needed for in vivo function of the enzyme, but not needed for cleavage activity. C-terminally truncated enzymes had similar strand passage activity to full length enzymes, but the presence of the opposite C-terminal domain had a large effect, with the topoIIα-CTD increasing activity, and the topoIIβ-CTD decreasing activity.
In vivo complementation data show that the topoIIα C-terminal domain is needed for growth, but the topoIIβ isoform is able to support low levels of growth without a C-terminal domain. This may indicate that topoIIβ has an additional localisation signal. In vitro data suggest that, while the lack of any C-terminal domain has little effect on activity, the presence of either the topoIIα or β C-terminal domain can affect strand passage activity. Data indicates that the topoIIβ-CTD may be a negative regulator. This is the first report of in vitro data with chimeric human topoIIs.
Citation: Meczes EL, Gilroy KL, West KL, Austin CA (2008) The Impact of the Human DNA Topoisomerase II C-Terminal Domain on Activity. PLoS ONE 3(3): e1754. https://doi.org/10.1371/journal.pone.0001754
Academic Editor: Adam Yuan, Temasek Life Sciences Laboratory, Singapore
Received: November 21, 2007; Accepted: December 27, 2007; Published: March 12, 2008
Copyright: © 2008 Meczes et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was funded by the North East Cancer Research Campaign (NECRC), the Biotechnology and Biological Sciences Research Council (BBSRC), and Newcastle University.
Competing interests: The authors have declared that no competing interests exist.
Type II DNA topoisomerases (topos II) are essential enzymes that resolve topological problems with DNA that arise during processes such as DNA replication and transcription, and chromosome segregation. Their mechanism involves the passing of one DNA duplex through a transient covalent break in a second duplex, in an ATP-dependent reaction. Humans have two topoisomerase II isoforms, α and β, which, while similar, have distinct patterns of expression and are thought to have different cellular roles . Human topoIIα is thought to be the isoform primarily involved with DNA replication and chromosome segregation, while human topoIIβ has recently been implicated in transcriptional regulation –.
While topoII isoforms show a high degree of sequence homology, approximately 70% between human topoII α and β, this is mainly in the N-terminal three-quarters of the protein sequence where the two catalytic centres are located. The C-terminal quarter of the protein, while always highly charged, shows much more sequence diversity. The C-terminal domain has been shown to be vital for cell viability. However, as C-terminal truncations are active in vitro, the essential nature of the C-terminal domain is thought to be linked to regulation .
Phosphorylation is a major form of regulation of human topoIIs, and has been shown to affect activity. Most modification sites are in the C-terminal domain, although a modification site at human topoIIα residue S29 that is a substrate for protein kinase C has also been identified . Phosphorylation sites have been identified in S. cerevisiae topoII . Some of these modifications are cell cycle specific, with modification at S1354, S1357, S1364 and T1366 being increased during mitosis, and modification at positions T1259, S1273, S1270 and S1267 increasing in G1 .
Considering the human enzymes, phosphorylation sites have been identified in topoIIα, with casein kinase II (CKII) being a principle kinase. αS1524 was identified as a principle phosphorylation site . Several studies have linked human topoIIα phosphorylation to events at mitosis, and phosphorylation of αS1212 has been shown to occur at only at mitosis . Phosphorylation has been suggested to activate topoII for chromatid segregation in anaphase , and CKII mediated αS1469 phosphorylation has been linked to chromatin condensation at prophase . Additionally, αT1342 has been proposed to regulate mitotic functions , although another study has shown this to be phosphorylated throughout the cell cycle . Human topoisomerase II phosphorylation sites, both cell cycle dependent and independent, are reviewed in Austin and Marsh, 1998 .
Modification by SUMO (small ubiquitin-like modifier) has also been linked to topoII regulation, with work in S. cerevisiae indicating that the topoII major modification sites (K1220, K1246/K1247 and K1277/K1278) again lie in the C-terminal domain . In S. cerevisiae topoII, SUMO modification has been linked to chromosome stability, with modified topoII enriched at centromeric regions . Additionally, topoII was found to be SUMO modified at metaphase, and was proposed to be essential for centromeric cohesion .
Work with human topo enzymes has shown that SUMO is rapidly conjugated to topoI, topoIIα and topoIIβ in response to DNA damage –. Additionally, after exposure to topoisomerase II inhibitor ICRF-193 human topoIIβ, but not topoIIα was selectively degraded by the proteasome, an activity that was abolished when the SUMO conjugating enzyme Ubc9 was knocked out. This implies that the degradation was linked to SUMO modification, and that this modification differs between the topoIIα and topoIIβ isoforms .
A major regulatory feature found in the C-terminal domains of topoIIs are nuclear localisation and nuclear export sequences (NLSs and NESs respectively). Without these signals, the enzyme is not able to localise to the nucleus, where it is essential during DNA replication, and cell viability is thus diminished or lost. Considering the human topoII isoforms, in topoIIα a strong NLS is found at 1454–1497 –, and consistent with this, a mutant lacking residues 1490–1492 is unable to locate to the nucleus . Another moderate NLS has been found in topoIIα at 1259–1296 . In topoIIβ nuclear localisation signals have been found in the C-terminal domain, with two strong NLSs have been identified at 1522–1548 and 1538–1573, with a weaker sequence at 1294–1332 , . Studies with isolated topoIIα and β C-terminal domains tagged with Yellow Fluorescent Protein showed that the two were differently localised in the nucleus .
In human topoIIα an NES was initially localised to the region 1018–1088 , and subsequently this was narrowed down to two sequences, 1017–1028 and 1054–1066, the latter of which is the stronger sequence . In topoIIβ an NES sequence has been idenfied between residues 1034–1044 .
Work with chimeric ‘tail swap’ proteins, where the C-terminal domain of topoIIα or topoIIβ is joined to the main body of the enzyme belonging to the opposite isoform, has been reported. A chimera of murine topoII consisting of the body of topoIIα and the tail of topoIIβ showed that this protein was unable to support growth . Conversely, a study examining the ability of human topoII chimeric tail swap proteins to rescue topoIIα cells in vivo found that the chimeric proteins, particularly those bearing the topoIIα C-terminal domain, could support growth .
To assess whether the C-terminal domain, as the most diverse part of the protein, impacted the relative activities of topoIIα and β, we aimed to construct and characterise in vitro and in vivo full length and C-terminally truncated forms of human topoIIα and β, and two ‘tail swap’ chimeric proteins where the C-terminal domain of each isoform is linked to the main sequence of the opposite isoform. In contrast to a recently published study describing the in vivo characterisation of tail swap proteins, where the C-terminal domain boundary was determined by alignment , the constructs described here have boundaries chosen based on those determined by limited proteolysis . The construction process, as well as subsequent characterisation, is reported here.
Materials and Methods
All chemicals were purchased from Sigma, BDH or Boehringer Mannheim. Restriction enzymes were purchased from NBL Gene Sciences Ltd, New England Biolabs, or Pharmacia Biotech. T4 Ligase was purchased from Gibco BRL. Etoposide was a gift from Prof. H. Newell, NICR, Newcastle, UK. mAMSA, Merbarone and Suramin were obtained from the Drug Synthesis and Chemistry branch, NCI, Bethesda, MD. Quercetin, Quercetagetin, Myricetin and Baicalein were provided by Prof. L.M. Fisher. mAMCA, DACA and Cl-DACA were provided by Prof. B Baguley, Auckland Cancer Society, New Zealand. All other cytotoxics were purchased from Sigma.
Plasmids and Yeast Strains
S. cerevisiae strain JEL1 was used for overexpression of proteins. Yeast strain JN394t2-4, a temperature sensitive strain that is viable at 25°C but non-viable at 35°, was used in complementation analysis.
All of the plasmids encoding topoII isoforms express protein under the control of the GAL1 promoter, and have a URA3 marker gene, the yeast 2μ plasmid replication origin and the β-lactamase gene and replication origin of E. coli pBR322 are also present. Plasmid YEpWob6, used to express recombinant human topoIIα, encodes the first 5 amino acids of S. cerevisiae topoII fused to residues 29–1531 of human topoIIα. Plasmid YEphTOP2β encodes recombinant human topoIIβ with the S165R mutation, with residues 46–1621 fused to the first 5 amino acids of S. cerevisiae topoII –. Plasmid YEphTOP2βKLM encodes recombinant wild type topoIIβ residues 46–1621 (without mutation S165R) fused to the first 5 of S. cerevisiae topoII. Plasmid intermediates used in the cloning process to construct C-terminal truncations and tail swaps are described in the ‘Results’ section and figure legends.
Construction of mutant plasmids
Plasmids encoding truncated topoIIα and topoIIβ, as well as two ‘tail swap’ chimeric proteins with the opposite C-terminal domain fused to the main coding sequence were constructed as described in ‘Results’. In all cases restriction digests were carried out according to manufacturer's instructions. Fragments were separated by agarose gel electrophoresis and then purified using a QIAquick Gel extraction spin column. Ligations were then carried out using T4 ligase and the manufacturer's buffers, incubating with 0.5mM ATP for 16 hours at 4°C.
Tail swap mutants were constructed with triple cloning procedures, then the junction sites were confirmed by sequencing both strands with dideoxy DNA sequencing using appropriate primers and a Sequenase version 2.0 DNA sequencing kit (Amersham).
Preparation of protein
Recombinant human topoIIα and β proteins were expressed and purified as described previously , . ATP dependent and independent relaxation assays were done with purified fractions to identify those free of topoI activity.
In vitro assays
In vivo assays
Complementation assays were carried out in a temperature sensitive yeast strain JN394t2-4, and plasmids encoding topoIIα and topoIIβ (WT and S165R) full length, C-terminally truncated or chimeric proteins. Yeast were grown in Ura- selective media at the permissive temperature (25°C) to an OD600 of 1, and then serially diluted in sterile microtitre trays. These cultures were then transferred to plates with an aluminium replicator, then incubated at the permissive, semi-permissive and non-permissive (25°C, 30°C or 35°C) temperatures respectively on glucose containing media, then growth was scored.
Construction of plasmids
Truncations at the 3′ end of the coding sequence of topoIIα and topoIIβ (S165R) were constructed from plasmids YEpWob6 and YEphTOP2β respectively. The truncated topoIIα plasmid encodes residues 29–1242 and the truncated topoIIβ plasmid encodes residues 46–1263, these being the start of the C-terminal domains as determined by limited proteolysis experiments , , . Schematics of these plasmids are shown in Figure 1.
Shown are the topoII sequence boundaries, the GAL1 promoter, the 2μ replication origin, the URA3 marker gene, and an ampicillin resistance gene. Restriction sites used in plasmid construction are indicated.
In the construction of C-terminally truncated topoIIα, PCR was used to introduce a PstI restriction site in the human topoIIα coding sequence and then to generate the full coding sequence. A fragment was generated between codon 791 (over a KpnI site) and codon 1244 (over the PstI site introduced above) of topoIIα. The PCR product was cloned into a Bluescript plasmid with a XhoI site immediately 3′ to the 792–1242 fragment, then excised by digestion with KpnI and XhoI. This was then cloned into the YEpWob6 plasmid, replacing the fragment 792–1531.
In the construction of C-terminally truncated topoIIβ, multiple internal restriction sites in the topoIIβ sequence meant that a complex cloning procedure was necessary. A fragment containing topoIIβ residues 900–1263 was excised and cloned into a Bluescript plasmid between BamHI and PstI restriction sites, this having a XhoI site 36 residues downstream of the PstI site. A fragment between BamHI to the XhoI beginning at codon 900 was excised and cloned into a vector containing topoIIβ codons 46–899 plus the YEp backbone.
Plasmids encoding chimeric ‘tail-swap’ proteins were created using a triple ligation approach and PCR to generate unique sites. Construction of the topoIIα+β tail plasmid, encoding topoIIα residues 30–1244 fused to topoIIβ residues 1263–1621, is illustrated in figure 2A. Likewise, construction of the topoIIβ (S165R)+α tail plasmid, encoding topoIIβ residues 46–1263 fused to topoIIα residues 1244–1531, is illustrated in figure 2B.
A–construction of topoIIα+β tail. TopoIIα fragments 30–791 and 792–1244 and topoIIβ fragment 1263–1621 were generated using restriction digests as shown, then ligated to give the final construct shown. B–construction of topoIIβ+α tail. TopoIIβ fragments 46–899 and 900–1263 and topoIIα fragment 1244–1531 were generated with restriction digests as indicated, then ligated to give the final construct shown .
All topoIIβ constructs containing the S165R mutation were changed to give wild type sequence by site directed mutagenesis using a Chameleon kit (stratagene) according to manufacturer's instructions.
TopoII protein activities
The decatenation activity of wild type topoIIα, C-terminally truncated topoIIα, and topoIIα+β tail was assayed. The values for 50% decatenation (D50), in ng of protein, are shown in figure 3A. There is no significant difference in D50 between topoIIα and its C-terminal truncation, with values of 4.5±1.3 and 6±1 ng of protein respectively. The topoIIα+β tail chimera however does show a significant reduction in decatenation as compared to full length topoIIα, with a D50 of 29±1 (p = 0.0008 in a two-tailed unpaired student t-test). This implies that while topoIIα can function perfectly well without a C-terminal domain, the β-tail on the topoIIα enzyme impedes activity.
A: Decatenation activity of all proteins, each column is the mean of at least two independent experiments. Standard errors are shown, with significant difference from full length enzyme marker with ‘***’. B: Representative cleavage experiment with 4.3 kb linearised pBR322 DNA with all proteins in the presence of mitoxantrone. TopoIIβ proteins in this case carry the S165R mutation.
It is possible that the observed reduction in catalytic activity seen with the topoIIα+β tail chimeric protein was an artefact, caused by the insertion of the tail into the enzyme altering a property such as conformation. A chimeric protein of topoIIβ mutant S165R, known to give a 5-fold reduction in decatenation activity , fused to the α-tail, was used to address this concern. If the insertion of the tail per se impedes catalytic activity, then the topoIIβ (S165R)+α tail would be expected to have still lower decatenation activity than topoIIβ(S165R). As shown in figure 3A the presence of the α tail gave no reduction in the decatenation activity of topoIIβ(S165R). As with topoIIα, removing the topoIIβ C-terminal domain gave no significant difference in activity, with D50 values of 50±8 and 60±8 ng protein for topoIIβ(S165R) and C-terminally truncated topoIIβ(S165R) respectively. The activity of the topoIIβ(S165R)+α tail chimera was increased very slightly with a D50 of 41.5±0.5 ng protein, however this difference was not statistically significant (p = 0.4001, figure 3A).
Complementation analysis of isoforms
To assess the in vivo functional activity of the truncated and chimeric proteins, complementation experiments were carried out in the temperature sensitive yeast strain JN394t2-4. Data are shown in table 1. All of the topoIIβ (S165R) plasmids were unable to complement at the restrictive temperature, consistent with previous results . Both wild type topoII isoforms supported good growth as expected. The truncated topoIIα and topoIIα+β tail proteins were unable to complement, but interestingly the truncated topoIIβ and topoIIβ+α tail proteins were able to support low levels of growth.
Cleavage assays with tail swaps
The cleavage activity of each of the wild type and C-terminal truncation mutant proteins was assessed in an end-labelled cleavage assay, in the presence and absence of drug. While the topoIIβ proteins here had mutation S165R this has been shown to have no effect on cleavage under the conditions used . In the absence of drug all six proteins gave cleavage at similar sites (data not shown). While topoIIα gave cleavage at more sites than topoIIβ this difference wasn't statistically significant. The same pattern was seen with the truncated and tail swap proteins, with topoIIα proteins generally giving slightly more cleavage than their topoIIβ counterpart, however this difference wasn't significant.
Drug stimulated cleavage was assayed with flavonoids quercetin, quercetagetin, myricetin, and baicalein, acridines mAMSA and mAMCA, etoposide and mitoxantrone. No difference in cleavage pattern between proteins was seen with drugs with the exception of truncated topoIIβ which promoted cleavage with mAMCA sites corresponding to a combination of topoIIβ and topoIIα. Additionally truncated topoIIβ promoted no cleavage with mitoxantrone (figure 3B), and topoIIα+β tail promoted cleavage with quercetagetin at sites more in common with topoIIβ than topoIIα. This indicates that the C-terminal domain of topoIIβ has a role in the determination of cleavage sites with certain drugs.
Human topoII isoforms α and β, while enzymatically similar in vitro, have been shown to have different cellular roles. While topoIIα is thought to be the isoform primarily responsible for DNA segregation, topoIIβ has recently been linked to transcription initiation –. Here we report the construction and characterisation of recombinant truncated and tail swap chimeric proteins. Whilst truncated human topoIIα has been reported previously, the point of truncation in this case was chosen to align with a viral topoII lacking the C-terminal domain . C-terminal truncations of topoIIα and β reported here were based on domain organisation as determined by the cleavage sites in limited proteolysis studies . Likewise, human chimeric tail swap proteins have been described and their in vivo function reported, but these also had domain boundaries based on alignment rather than proteolysis sites . Here we report, for the first time, the creation of C-terminally truncated recombinant human topoII proteins based on domain structure indicated by limited proteolysis experiments. Furthermore, we have also created chimeric recombinant human topoII tail swap proteins based on this definition of the C-terminal domain. Additionally, this manuscript is the first report of characterisation of the in vitro function of human chimeric tail swap proteins.
Complementation analysis showed, as previously reported, that the S165R mutant proteins were not functional in vivo . The topoIIα C-terminal truncation couldn't support growth, in accordance with previous work showing that the loss of the C-terminal domain, and the localisation signals within it, are detrimental to growth , . The topoIIα+β chimera was also unable to support growth suggesting that the β C-terminal domain is unable to restore the localisation of the enzyme, or perhaps that the topoIIβ C-terminal domain has a different function to the topoIIα C-terminal domain. This would be consistent with previous experiments showing that human topoIIα preferentially relaxes positive supercoils, whereas topoIIβ showed no preference . This result is in contrast to a study with a murine topoIIα+β protein which was able to support growth in S. cerevisiae strain NAY113 . This difference could be species specific, or due to differences in the definition of the start of the C-terminal domain. In the murine study the last 444 amino acids of the β-tail were used to replace the equivalent region on topoIIα, in contrast to 358 residues here, with 356 amino acids of the topoIIα tail lost in the murine chimera in contrast to 289 here.
Perhaps more surprising is that topoIIβ truncated protein and topoIIβ+α tail protein can support low levels of growth, implying that some localisation to the nucleus is still present. Known nuclear localisation signals are shown in figure 4 and, with the exception of S. pombe topoII which also has an N-terminal signal, all sequences are found in the C-terminal domain , , , . It is therefore unclear why the truncated topoIIβ protein is able to support low levels of growth (and hence localise, albeit inefficiently, to the nucleus), but it is possible that this is due to a presently unknown mechanism, perhaps linked to topoIIβ specific modification, or an unidentified NLS specific to topoIIβ. This would be consistent with previous work that showed that the topoIIα and β C-terminal domains were differently localised .
Above each bar are the residue numbers at the start and end of the primary sequence, plus the point equating to the start of the C-terminal domain (indicated by arrows), as determined by limited proteolysis where known, and by alignment with this point where this is not known. Also shown are active site tyrosines (Y), known nuclear localisation sequences (NLS-dark grey) and known nuclear export sequences (NES–light grey). NLS sequences have been identified in human topoIIα (1259–1296, 1454–1497 ), human topoIIβ (1294–1332, 1522–1548, 1538–1573 ), S. cerevisiae topoII (1227–1242 ) and S. pombe (26–44, 1227–1242, 1322–1339, 1335–1357 ). NES sequences have been identified in human topoIIα (1017–1028, 1054–1066 ) and human topoIIβ (1034–1044 ).
A previous study into human chimeric enzymes found that proliferation of topoIIα knockout human cells was supported in all cases by enzymes bearing the topoIIα C-terminal domain, but that proliferation was only supported rarely and when protein was expressed in large quantities for enzymes bearing the topoIIβ C-terminal domain. The relative levels of growth support are consistent with the data presented here (where the topoIIα C-terminal domain chimera supports low levels of growth and the topoIIβ C-terminal domain chimera supports no growth at all), although the levels of growth differ, perhaps because of differences in the experimental systems or the sensitivity of methods .
The decatenation data imply that the topoII C-terminal domain is involved in the modulation of catalytic activity in the two human isoforms. The truncated topoII proteins had no difference in in vitro decatenation activity as compared to their full length counterparts suggesting that the C-terminal domain is not necessary for in vitro activity, which is consistent with previous data , , . While the absence of the C-terminal domain for topoIIα or β had no effect on strand passage activity, the presence of the C-terminal domain from the opposite isoform had a noticeable effect with a clear trend emerging. The presence of the β-tail on the topoIIα isoform core gave a statistically significant decrease in strand passage activity compared to the native topoIIα protein, and the presence of the α-tail on the topoIIβ isoform core gave an increase in activity compared to the native topoIIβ protein, although this time not significant, towards that of the native topoIIα protein. As the truncated forms of each protein had no difference in activity when compared to the full length, this implies that it is the presence of the α or β tail that is important for the level of strand passage, acting as a regulator. In this case of the topoIIβ C-terminal domain particularly, this regulation (negative in this case), is quite striking. While it can't formally be excluded that the reduced activity of the chimeric topoIIα+β tail protein is due to the tail swap process, this seems unlikely, as if the process itself reduced activity this should also be seen with the topoIIβ(S165R)+α tail protein. In fact the opposite is seen, with the absence of the β-CTD seeming to ‘release’ the enzyme activity a little and increase the rate of decatenation.
The regulation of catalytic activity by the C-terminal domain could be mediated via differential modification, for instance phosphorylation or SUMOylation, or could be linked to the extensive differences in primary sequence between the two C-terminal domains.
Observations reported previously support the hypothesis that topoIIα and β C-terminal domains are important in differential regulation of the isoforms. All of the SUMO modification sites identified to date have been located in the C-terminal domain of topoII . SUMO conjugation to topoI, topoIIα and topoIIβ has also been linked to the human cellular response to DNA damage –. However, differential degradation of topoIIβ but not topoIIα was observed in response to treatment with ICRF-193, strongly suggesting that the two isoforms are regulated differently by SUMO modification .
An analysis of the theoretical protein parameters of full length and truncated topoIIα and topoIIβ, and their tail swap derivatives, is shown in table 2. What is immediately obvious is that, while the full length and truncated topoII isoforms all have similar theoretical pIs, with full length topoIIβ having a slightly lower theoretical pI, the theoretical pI of the isolated C-terminal domain of topoIIβ is considerably lower than other topoII proteins. This is linked to the higher number of acidic residues compared to basic residues seen with this fragment. Unsurprisingly, the α+β tail protein has a lower theoretical pI than full length topoIIα, and the β+α tail protein has a higher theoretical pI than the full length topoIIβ protein, but a similar pI to the truncated topoIIβ protein . It would thus appear that the lower theoretical pI of the β-CTD acts to lower the pI of the core protein to which it is attached. Conversely, the addition of the α-CTD has little effect on either core protein's theoretical pI.
Cleavage data suggests that the C-terminal domain is not generally involved in cleavage, although there was a consistent, yet non-significant, increase in cleavage with the topoIIα derived proteins. Most drugs showed no difference in drug-stimulated cleavage patterns between topoIIα and β, implying that the C-terminal domain has no impact on the action of these drugs, consistent with previous work showing that topoIIα and topoIIβ cleave at similar sites . There were exceptions to this rule however, for example the truncated topoIIβ showed no cleavage with mitoxantrone, showing that some drugs may have specific interactions that involve the C-terminal domain of human topoIIβ.
In summary, we report the construction of C-terminally truncated and chimeric human topoII enzymes, and show that the C-terminal domain impacts on the activity of the two human isoforms. Further characterisation of human topoIIα and topoIIβ, perhaps by investigating the effect of SUMOylation on either isoform, or the cellular localisation of these tail swap proteins, will be needed to elucidate their different interactions with DNA substrates and functional roles in cells.
Conceived and designed the experiments: CA. Performed the experiments: EM KW. Analyzed the data: KG EM KW. Contributed reagents/materials/analysis tools: CA. Wrote the paper: KG.
- 1. Corbett KD, Berger JM (2004) Structure, Molecular Mechanisms and Evolutionary Relationships in DNA Topoisomerases. Annu Rev Biophys Biomol Struct 33: 95–118.KD CorbettJM Berger2004Structure, Molecular Mechanisms and Evolutionary Relationships in DNA Topoisomerases.Annu Rev Biophys Biomol Struct3395118
- 2. Ju B-G, Lunyak VV, Perissi V, Garcia-Bassets I, Rose DW, et al. (2006) A Topoisomerase IIβ-Mediated dsDNA Break Required for Regulated Transcription. Science 312: 1798–1802.B-G JuVV LunyakV. PerissiI. Garcia-BassetsDW Rose2006A Topoisomerase IIβ-Mediated dsDNA Break Required for Regulated Transcription.Science31217981802
- 3. Kitagawa H, Fujiki R, Yoshimura K, Mezaki Y, Uematsu Y, et al. (2003) The chromatin-remodelling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome. Cell 113: 905–917.H. KitagawaR. FujikiK. YoshimuraY. MezakiY. Uematsu2003The chromatin-remodelling complex WINAC targets a nuclear receptor to promoters and is impaired in Williams syndrome.Cell113905917
- 4. Ju B-G, Solum D, Song EJ, Lee K-J, Rose DW, et al. (2004) Activating the PARP-1 sensor component of the groucho/TLE1 corepressor complex mediates a CaMKinaseIIdelta-dependent neurogenic gene activation pathway. Cell 119: 815–829.B-G JuD. SolumEJ SongK-J LeeDW Rose2004Activating the PARP-1 sensor component of the groucho/TLE1 corepressor complex mediates a CaMKinaseIIdelta-dependent neurogenic gene activation pathway.Cell119815829
- 5. Austin CA, Marsh KL (1998) Eukaryotic DNA topoisomerase IIβ. Bioessays. 20: 215–226.CA AustinKL Marsh1998Eukaryotic DNA topoisomerase IIβ.Bioessays.20215226
- 6. Wells NJ, Fry AM, Guano F, Norbury C, Hickson ID (1995) Cell cycle phase-specific phosphorylation of human topoisomerase II alpha: Evidence of a role for protein kinase C. J. Biol. Chem. 270: 28357–28363.NJ WellsAM FryF. GuanoC. NorburyID Hickson1995Cell cycle phase-specific phosphorylation of human topoisomerase II alpha: Evidence of a role for protein kinase C. J. Biol. Chem.2702835728363
- 7. Cardenas ME, Dong Q, Glover CVC, Gasser SM (1992) Casein kinase II phosphorylates the eukaryotic-specific C-terminal domain of topoisomerase II in vivo. The EMBO J 11: 1785–1796.ME CardenasQ. DongCVC GloverSM Gasser1992Casein kinase II phosphorylates the eukaryotic-specific C-terminal domain of topoisomerase II in vivo.The EMBO J1117851796
- 8. Wells NJ, Addison CM, Fry AM, Ganapathi R, Hickson ID (1994) Serine 1524 Is a Major Site of Phosphorylation on Human Topoisomerase IIα Protein in vivo and is a Substrate for Casein Kinase II in vitro. J Biol Chem 269: 29746–29751.NJ WellsCM AddisonAM FryR. GanapathiID Hickson1994Serine 1524 Is a Major Site of Phosphorylation on Human Topoisomerase IIα Protein in vivo and is a Substrate for Casein Kinase II in vitro.J Biol Chem2692974629751
- 9. Ishida R, Takashima R, Koujin T, Shibata M, Nozaki N, et al. (2001) Mitotic Specific Phosphorylation of Serine-1212 in Human DNA Topoisomerase IIα. Cell Struct Func 26: 215–226.R. IshidaR. TakashimaT. KoujinM. ShibataN. Nozaki2001Mitotic Specific Phosphorylation of Serine-1212 in Human DNA Topoisomerase IIα.Cell Struct Func26215226
- 10. Heck MMS, Hittelman WN, Earnshaw WC (1989) In vivo phosphorylation of the 170 kDa Form of Eukaryotic DNA Topoisomerase II: Cell Cycle Analysis. J Biol Chem 264: 15161–15164.MMS HeckWN HittelmanWC Earnshaw1989In vivo phosphorylation of the 170 kDa Form of Eukaryotic DNA Topoisomerase II: Cell Cycle Analysis.J Biol Chem2641516115164
- 11. Escargueil AE, Plisov SY, Cochet C, Larsen AK (2000) Mitotic Phosphorylation of DNA Topoisomerase IIα by Protein Kinase CK2 Creates the MPM-2 Phosphoepitope on Ser-1469. J Biol Chem 275: 34710–34718.AE EscargueilSY PlisovC. CochetAK Larsen2000Mitotic Phosphorylation of DNA Topoisomerase IIα by Protein Kinase CK2 Creates the MPM-2 Phosphoepitope on Ser-1469.J Biol Chem2753471034718
- 12. Daum JR, Gorbsky GJ (1998) Casein Kinase II Catalyses a Mitotic Phosphorylation on Threonine 1342 of Human DNA Topoisomerase IIα, Which Is Recognised by the 3F3/2 Phosphoepitope Antibody. J Biol Chem 273: 30622–30629.JR DaumGJ Gorbsky1998Casein Kinase II Catalyses a Mitotic Phosphorylation on Threonine 1342 of Human DNA Topoisomerase IIα, Which Is Recognised by the 3F3/2 Phosphoepitope Antibody.J Biol Chem2733062230629
- 13. Ishida A, Iwai M, Marsh KL, Austin CA, Yano T, et al. (1996) Threonine 1342 in Human Topoisomerase IIα Is Phosphorylated Throughout the Cell Cycle. J Biol Chem 271: 30077–30082.A. IshidaM. IwaiKL MarshCA AustinT. Yano1996Threonine 1342 in Human Topoisomerase IIα Is Phosphorylated Throughout the Cell Cycle.J Biol Chem2713007730082
- 14. Bachant J, Akasabas A, Blat Y, Kleckner N, Elledge SJ (2002) The SUMO-1 Isopeptidase Smt4 is Linked to Centromeric Cohesion through SUMO-1 Modification of DNA Topoisomerase II. Mol Cell 9: 1169–1182.J. BachantA. AkasabasY. BlatN. KlecknerSJ Elledge2002The SUMO-1 Isopeptidase Smt4 is Linked to Centromeric Cohesion through SUMO-1 Modification of DNA Topoisomerase II.Mol Cell911691182
- 15. Takahashi Y, Yong-Gonzalez V, Kikuchi Y, Strunnikov A (2006) SIZ1/SIZ2 Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II. Genetics 172: 783–794.Y. TakahashiV. Yong-GonzalezY. KikuchiA. Strunnikov2006SIZ1/SIZ2 Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II.Genetics172783794
- 16. Mao Y, Desai SD, Liu LF (2000) SUMO-1 Conjugation to Human DNA Topoisomerase II Isozymes. J Biol Chem 275: 26066–26073.Y. MaoSD DesaiLF Liu2000SUMO-1 Conjugation to Human DNA Topoisomerase II Isozymes.J Biol Chem2752606626073
- 17. Azuma Y, Arnaoutov A, Dasso M (2003) SUMO-2/3 regulates topoisomerase II in mitosis. J Cell Biol 163: 477–487.Y. AzumaA. ArnaoutovM. Dasso2003SUMO-2/3 regulates topoisomerase II in mitosis.J Cell Biol163477487
- 18. Isik S, Sano K, Tsutsui K, Seki M, Enomoto T, et al. (2003) The SUMO pathway is required for selective degradation of DNA topoisomerase IIβ induced by a catalytic inhibitor ICRF-193. FEBS Lett 546: 374–378.S. IsikK. SanoK. TsutsuiM. SekiT. Enomoto2003The SUMO pathway is required for selective degradation of DNA topoisomerase IIβ induced by a catalytic inhibitor ICRF-193.FEBS Lett546374378
- 19. Mirski SEL, Gerlach JH, Cummings HJ, Zirngibl R, Greer PA, et al. (1997) Bipartite Nuclear Localisation Signals in the C-terminus of Human Topoisomerase IIα. Exp Cell Res 237: 452–455.SEL MirskiJH GerlachHJ CummingsR. ZirngiblPA Greer1997Bipartite Nuclear Localisation Signals in the C-terminus of Human Topoisomerase IIα.Exp Cell Res237452455
- 20. Mirski SE, Gerlach JH, Cole SP (1999) Sequence determinants of nuclear localisation in the alpha and beta isoforms of human topoisomerase II. Exp. Cell Res. 251: 329–339.SE MirskiJH GerlachSP Cole1999Sequence determinants of nuclear localisation in the alpha and beta isoforms of human topoisomerase II.Exp. Cell Res.251329339
- 21. Wessel I, Jensen PB, Falck J, Mirski SEL, Cole SPC, et al. (1997) Loss of amino acids 1490Lys-Ser-Lys1492 in the COOH-Terminal region of topoisomerase IIα in Human Small Cell Lung Cancer cells selected for resistance to etoposide results in extranuclear enzyme localisation. Cancer Res 57: 4451–4454.I. WesselPB JensenJ. FalckSEL MirskiSPC Cole1997Loss of amino acids 1490Lys-Ser-Lys1492 in the COOH-Terminal region of topoisomerase IIα in Human Small Cell Lung Cancer cells selected for resistance to etoposide results in extranuclear enzyme localisation.Cancer Res5744514454
- 22. Cowell IG, Willmore E, Chalton D, Marsh KL, Jazrawi E, et al. (1998) Nuclear distribution of human DNA topoisomerase IIbeta: a nuclear targeting signal resides in the 116-residue C-terminal tail. Exp. Cell Res. 243: 232–240.IG CowellE. WillmoreD. ChaltonKL MarshE. Jazrawi1998Nuclear distribution of human DNA topoisomerase IIbeta: a nuclear targeting signal resides in the 116-residue C-terminal tail.Exp. Cell Res.243232240
- 23. Sakaguchi A, Akashi T, Kikuchi A (2001) A distinct subcellular localisation of mammalian DNA topoisomerase IIbeta in yeast. Biochem. Biophys. Res. Comm. 283: 876–882.A. SakaguchiT. AkashiA. Kikuchi2001A distinct subcellular localisation of mammalian DNA topoisomerase IIbeta in yeast.Biochem. Biophys. Res. Comm.283876882
- 24. Mirski SE, Bielawski JC, Cole SP (2003) Identification of functional nuclear export sequences in human topoisomerase II alpha and beta. Biochem. Biophys. Res. Commun. 306: 905–911.SE MirskiJC BielawskiSP Cole2003Identification of functional nuclear export sequences in human topoisomerase II alpha and beta.Biochem. Biophys. Res. Commun.306905911
- 25. Turner JG, Engel R, Derderian JA, Jove R, Sullivan DM (2004) Human topoisomerase IIα nuclear export is mediated by two CRM-1-dependent nuclear export signals. J Cell Sci 117: 3061–3071.JG TurnerR. EngelJA DerderianR. JoveDM Sullivan2004Human topoisomerase IIα nuclear export is mediated by two CRM-1-dependent nuclear export signals.J Cell Sci11730613071
- 26. Adachi N, Miyaike M, Kato S, Kanamara R, Koyama H, et al. (1997) Cellular distribution of mammalian DNA topoisomerase II is determined by its catalytically dispensable C-terminal domain. Nucleic Acids Res. 25: 3135–3142.N. AdachiM. MiyaikeS. KatoR. KanamaraH. Koyama1997Cellular distribution of mammalian DNA topoisomerase II is determined by its catalytically dispensable C-terminal domain.Nucleic Acids Res.2531353142
- 27. Linka RM, Porter AC, Volkov A, Mielke C, Boege F, et al. (2007) C-terminal regions of topoisomerase IIalpha and IIbeta determine isoforms-specific functioning of the enzymes in vivo. Nucleic Acids Res. 35: 810–822.RM LinkaAC PorterA. VolkovC. MielkeF. Boege2007C-terminal regions of topoisomerase IIalpha and IIbeta determine isoforms-specific functioning of the enzymes in vivo.Nucleic Acids Res.35810822
- 28. Austin CA, Marsh KL, Wasserman RA, Willmore E, Sayer PJ, et al. (1995) Expression, Domain Structure and Enzymatic Properties of an Active Recombinant Human DNA Topoisomerase IIβ. J. Biol. Chem. 270: 15739–15746.CA AustinKL MarshRA WassermanE. WillmorePJ Sayer1995Expression, Domain Structure and Enzymatic Properties of an Active Recombinant Human DNA Topoisomerase IIβ.J. Biol. Chem.2701573915746
- 29. Gilroy KL, Leontiou C, Padget K, Lakey JH, Austin CA (2006) mAMSA resistant human topoisomerase IIβ mutation G465D has reduced ATP hydrolysis activity. Nucleic Acid Res. 34: 1597–1607.KL GilroyC. LeontiouK. PadgetJH LakeyCA Austin2006mAMSA resistant human topoisomerase IIβ mutation G465D has reduced ATP hydrolysis activity.Nucleic Acid Res.3415971607
- 30. Leontiou C, Lakey JH, Austin CA (2004) Mutation E522K in Human DNA Topoisomerase IIβ Confers Resistance to Methyl N-(4′-(9-acridinylamino)-phenyl)carbamate hydrochloride and Methyl N-(4′-(9-acridinylamino)-3-methoxy-phenyl)methane sulfonamide but Hypersensitivity to Etoposide. Mol. Pharm. 66: 430–439.C. LeontiouJH LakeyCA Austin2004Mutation E522K in Human DNA Topoisomerase IIβ Confers Resistance to Methyl N-(4′-(9-acridinylamino)-phenyl)carbamate hydrochloride and Methyl N-(4′-(9-acridinylamino)-3-methoxy-phenyl)methane sulfonamide but Hypersensitivity to Etoposide.Mol. Pharm.66430439
- 31. Wasserman RA, Austin CA, Fisher LM, Wang JC (1993) Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast. Cancer Res 53: 3591–3596.RA WassermanCA AustinLM FisherJC Wang1993Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast.Cancer Res5335913596
- 32. Meczes EL (1998) PhD ThesisEL Meczes1998PhD Thesis
- 33. Meczes EL, Marsh KL, Fisher LM, Rogers MP, Austin CA (1997) Complementation of temperature-sensitive topoisomerase II mutations in Saccharomyces cerevisiae by a human TOP2β construct allows the study of topoisomerase IIβ inhibitors in yeast. Cancer Chemother. Pharmacol. 39: 367–375.EL MeczesKL MarshLM FisherMP RogersCA Austin1997Complementation of temperature-sensitive topoisomerase II mutations in Saccharomyces cerevisiae by a human TOP2β construct allows the study of topoisomerase IIβ inhibitors in yeast. Cancer Chemother.Pharmacol.39367375
- 34. West KL, Turnbull RM, Willmore E, Lakey JH, Austin CA (2002) Characterisation of the DNA-dependent ATPase activity of human DNA topoisomerase IIβ: mutation of Ser165 in the ATPase domain reduces the ATPase activity and abolishes the in vivo complementation ability. Nucleic Acids Res. 30: 5416–5424.KL WestRM TurnbullE. WillmoreJH LakeyCA Austin2002Characterisation of the DNA-dependent ATPase activity of human DNA topoisomerase IIβ: mutation of Ser165 in the ATPase domain reduces the ATPase activity and abolishes the in vivo complementation ability.Nucleic Acids Res.3054165424
- 35. Dickey JS, Osheroff N (2005) Impact of the C-Terminal Domain of Topoisomerase IIα on the DNA Cleavage Activity of the Human Enzyme. Biochemistry 44: 11546–11554.JS DickeyN. Osheroff2005Impact of the C-Terminal Domain of Topoisomerase IIα on the DNA Cleavage Activity of the Human Enzyme.Biochemistry441154611554
- 36. Jensen S, Andersen AH, Kjeldsen E, Biersack H, Olsen EHN, et al. (1996) Analysis of Functional Domain Organization in Topoisomerase II from Humans and Saccharomyces cerevisiae. Mol. Cell Biol. 16: 3866–3877.S. JensenAH AndersenE. KjeldsenH. BiersackEHN Olsen1996Analysis of Functional Domain Organization in Topoisomerase II from Humans and Saccharomyces cerevisiae.Mol. Cell Biol.1638663877
- 37. McClendon AK, Rodriguez AC, Osheroff N (2005) Human topoisomerase IIalpha rapidly relaxes positively supercoiled DNA: implications for enzyme action ahead of replication forks. J. Biol. Chem. 280: 39337–39345.AK McClendonAC RodriguezN. Osheroff2005Human topoisomerase IIalpha rapidly relaxes positively supercoiled DNA: implications for enzyme action ahead of replication forks.J. Biol. Chem.2803933739345
- 38. Caron PR, Watt P, Wang JC (1994) The C-terminal domain of Saccharomyces cerevisiae DNA topoisomerase II. Mol. Cell. Biol. 14: 3197–3207.PR CaronP. WattJC Wang1994The C-terminal domain of Saccharomyces cerevisiae DNA topoisomerase II.Mol. Cell. Biol.1431973207
- 39. Shiozaki K, Yanagida M (1992) Functional dissection of the phosphorylated termini of fission yeast DNA topoisomerase II. J. Cell Biol. 119: 1023–1036.K. ShiozakiM. Yanagida1992Functional dissection of the phosphorylated termini of fission yeast DNA topoisomerase II.J. Cell Biol.11910231036
- 40. Greenwood Crenshaw D, Hsieh T-S (1993) Function of the Hydrophilic Carboxyl Terminus of Type II DNA Topoisomerase from Drosophila melanogaster: I In Vitro Studies. J. Biol. Chem. 268: 21328–21334.D. Greenwood CrenshawT-S Hsieh1993Function of the Hydrophilic Carboxyl Terminus of Type II DNA Topoisomerase from Drosophila melanogaster: I In Vitro Studies.J. Biol. Chem.2682132821334
- 41. Sengupta T, Mukherjee M, Das R, Das A, Majunider HK (2005) Characterisation of the DNA-binding domain and identification of the active site residue in the ‘GyrA’ half of Leishmania donovani topoisomerase II. Nucleic Acids Res. 33: 2364–2373.T. SenguptaM. MukherjeeR. DasA. DasHK Majunider2005Characterisation of the DNA-binding domain and identification of the active site residue in the ‘GyrA’ half of Leishmania donovani topoisomerase II.Nucleic Acids Res.3323642373
- 42. Marsh KL, Willmore E, Tinelli S, Cornarotti M, Meczes EL, et al. (1996) Amsacrine-Promoted DNA Cleavage Site Determinants for the Two Human DNA Topoisomerase II Isoforms α and β. Biochem. Pharmacol. 52: 1675–1685.KL MarshE. WillmoreS. TinelliM. CornarottiEL Meczes1996Amsacrine-Promoted DNA Cleavage Site Determinants for the Two Human DNA Topoisomerase II Isoforms α and β.Biochem. Pharmacol.5216751685
- 43. Gilroy KL (2007) PhD ThesisKL Gilroy2007PhD Thesis