Worldwide, grapes and their derived products have a large market. The cultivated grape species Vitis vinifera has potential to become a model for fruit trees genetics. Like many plant species, it is highly heterozygous, which is an additional challenge to modern whole genome shotgun sequencing. In this paper a high quality draft genome sequence of a cultivated clone of V. vinifera Pinot Noir is presented.
We estimate the genome size of V. vinifera to be 504.6 Mb. Genomic sequences corresponding to 477.1 Mb were assembled in 2,093 metacontigs and 435.1 Mb were anchored to the 19 linkage groups (LGs). The number of predicted genes is 29,585, of which 96.1% were assigned to LGs. This assembly of the grape genome provides candidate genes implicated in traits relevant to grapevine cultivation, such as those influencing wine quality, via secondary metabolites, and those connected with the extreme susceptibility of grape to pathogens. Single nucleotide polymorphism (SNP) distribution was consistent with a diffuse haplotype structure across the genome. Of around 2,000,000 SNPs, 1,751,176 were mapped to chromosomes and one or more of them were identified in 86.7% of anchored genes. The relative age of grape duplicated genes was estimated and this made possible to reveal a relatively recent Vitis-specific large scale duplication event concerning at least 10 chromosomes (duplication not reported before).
Sanger shotgun sequencing and highly efficient sequencing by synthesis (SBS), together with dedicated assembly programs, resolved a complex heterozygous genome. A consensus sequence of the genome and a set of mapped marker loci were generated. Homologous chromosomes of Pinot Noir differ by 11.2% of their DNA (hemizygous DNA plus chromosomal gaps). SNP markers are offered as a tool with the potential of introducing a new era in the molecular breeding of grape.
Citation: Velasco R, Zharkikh A, Troggio M, Cartwright DA, Cestaro A, Pruss D, et al. (2007) A High Quality Draft Consensus Sequence of the Genome of a Heterozygous Grapevine Variety. PLoS ONE 2(12): e1326. https://doi.org/10.1371/journal.pone.0001326
Academic Editor: Brian Dilkes, University of California at Davis, Genome Center, United States of America
Received: October 5, 2007; Accepted: November 21, 2007; Published: December 19, 2007
Copyright: © 2007 Velasco et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work has been funded by the Province of Trento, Italy. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Grapes (67 million t; http://faostat.fao.org/site/336/DesktopDefault.aspx) and their derivatives have a large and expanding worldwide market. Grapes can be grown at latitudes from 50°N to 40°S and up to 3,000 meters above sea level, with almost 98% of grape vineyards planted with Vitis vinifera L. ssp. sativa cultivars of Eurasian origin. Ever since the development of wine-making in Iran between 5,440 and 5,000 B.C. , wine has been an important component of many cultures. It has been celebrated by the Ecclesiates, by Horace, Goethe, Jefferson and the Nobel laureate J. C. Cela. A traditional icon of the Mediterranean diet , the grape has more recently been extensively cultivated in the New World and its cultivation is now moving to Asia. Given grape's content of resveratrol, quercitin and ellagic acid, grape products may contribute to reducing the incidence of cardiovascular and other diseases .
V. vinifera ssp. sativa, domesticated from the wild ssp. sylvestris , bears hermaphroditic self-fertilizing flowers. However, outbreeding by means of wind and insect pollination is the norm. As a result, cultivars are highly heterozygous and carry many deleterious recessive mutations . Inbreeding depression is severe, so that sterility often ensues from the second or third generation of selfing. All wild Vitis species have 38 chromosomes (n = 19) and most interspecies hybrids are fertile . The high chromosome number suggests a paleopolyploid state of the genome , an argument recently presented in the frame of a recent partial assembly of the grape genome  but still remaining controversial.
Grape has the potential to become a model organism for fruit trees. The species can be transformed  and micropropagated via somatic embryogenesis . Compared to other perennials, the genome size is relatively small, 475 Mb , similar to rice (Oryza sativa, 430 Mb; ), barrel medic (Medicago truncatula, 500 Mb, http://medicago.org/) and black cottonwood poplar (Populus trichocarpa, 465 Mb; ).
In this paper we report a high-quality draft sequence of the grapevine genome. The genome is derived from the Pinot Noir clone ENTAV 115, a variety grown in a range of soils for the production of red and sparkling wines. The sequence provides information on the overall organization, gene content and structural components of the DNA of the 19 LGs of V. vinifera. The Sanger sequencing method was used to generate 6.5X coverage of the genome. This has been integrated with sequence reads generated by a scalable, highly parallel sequencing by synthesis (SBS) method with throughput significantly greater than capillary electrophoresis. The 4.2X coverage provided by SBS was crucial in identifying polymorphic sites and in closing most of the gaps between DNA contigs. This is the first project which utilizes both the longer Sanger and shorter SBS methods to determine the sequence of a large eukaryotic genome.
Results and Discussion
Sequencing and assembly
The DNA of V. vinifera was extracted from young shoots and sequenced and assembled using the whole genome shotgun (WGS) method. Two techniques were adopted: the Sanger dye primer sequencing of paired reads  and 454 (SBS) of unpaired reads , which provided 6.5X and 4.2X genome coverage respectively (see Materials and Methods).
In order to develop criteria for assembly, a preliminary experiment was conducted to assess heterozygosity: it was found to correspond to approximately 1 SNP per 0.1 Kb and 1 in/del per 0.45 Kb (see Text S1). The assembly program  was accordingly modified to accept a specified level of mismatches in overlapping sequences (details in Materials and Methods and in Text S1). The program also incorporated information on clone size, which ranged from 2 to 130 Kb (Table S1).
The assembly started with unique sequences and progressively included sequences with a higher degree of repetitiveness. To avoid merging repeats into a single genomic sequence, the overlapping unique sequence contigs were merged if the rate of polymorphism did not exceed 2% and if the resulting sequence coverage of the overlap did not exceed 150% of the average coverage (see Text S1). These criteria were modified so that contigs with many supporting links were merged. In most cases, this procedure produced a correct assembly.
Applying the procedure to about 6.6 M reads from Sanger sequencing, 90.6% of which represented paired clone ends, 211,374 initial seed contigs of unique sequences were generated. By using long clone links with non-repetitive clone ends, seed contigs were ordered into metacontigs (ordered assembly of contigs, referred to as supercontigs or scaffolds in other publications). After the sequences were merged into 120,000 contigs, data were combined with 4.2 genome-equivalents of SBS data. This helped to identify polymorphic sites and closed 25% of the remaining gaps between contigs. After removal of 10,847 contigs composed only of tandemly repeated sequences and disposal of 7,003 contigs shorter than 1,000 bp, the iterative assembly produced 58,611 contigs (Figure S1 and Table S2) corresponding to 530.9 Mb of genomic DNA. 44,179 of the 58,611 contigs were assembled into 2,093 metacontigs and the remaining 14,432 contigs were singletons. The final assembled sequences are deposited at the EMBL/Genbank/DDBJ databases (accession numbers: AM423240-AM489403, data released 2006-12-19). Metacontig data are available at http://genomics.research.iasma.it. The removed contigs represented mostly centromeric and rRNA gene sequences. Based on their read coverage, their sizes were estimated as 14.5 Mb and 16.3 Mb, respectively.
Cultivated V. vinifera is highly heterozygous. As a result, many of the resulting contigs were consensus sequences derived from an alignment of the two haplotypes. The set of Pinot Noir chromosome pairs included a considerable number of haplotype-specific gaps (sequences present in one haplotype but not in the other; on this issue see also the ‘Pinot Noir genome structure and evolution’ section). The total length of the 1,042,174 identified gaps corresponded to 48.9 Mb. In some chromosomal regions, the two alternative haplotypes were too different for the algorithm employed during assembly to combine them into a single contig. Such separated contigs corresponded to the hemizygous DNA (22,061 contigs with the total length of 65.1 Mb). The total size of the genome represented by different homologous chromosomes can be estimated as twice the length of the sequences represented by the two haplotypes merged into a consensus (416.8×2 = 833.6 Mb), plus the sequence length represented by hemizygous DNA and gaps, respectively 65.1 and 48.9 Mb. After including the centromeric and rRNA regions (14.5×2+16.3×2 = 61.6 Mb), the size of the diploid genome was subsequently estimated to be 1,009.2 Mb, which gives an average 504.6 Mb per haploid genome (Table 1).
A region of 403,443 bp (preliminary experiment; see Text S1) was used to monitor the correctness of the assembly. Thirty four of the 37 contigs which mapped to the preliminary experiment sequence belonged to the metacontig assembled from the full genome sequence and were in the correct order. The remaining three contigs were not included because they contained repetitive clone links. Twenty two of the 36 boundaries between adjacent contigs were overlapping but not aligned due to large heterozygous inserts. The remaining 14 contig pairs corresponded to gaps: nine short gaps between 52 and 354 bp and five gaps larger than 500 bp. The largest gap (2.4 Kb) contained tandem repeats. Most of the gaps were associated to heterozygous inserts of repetitive elements. The total gap size, 8,067 bp, corresponded to about 2% of the region considered.
Metacontig integration into the genetic map
The next phase of the assembly involved positioning metacontigs in the genome using a genetic map developed at the Istituto Agrario di San Michele all'Adige (IASMA). Genetic mapping was based on 94 individuals derived from a F1 Syrah X Pinot Noir cross where the latter was the pollen donor. The map contained 1,006 markers , which were used both to anchor BAC contigs to a physical map (http://genomics.research.iasma.it) and to order metacontigs along linkage groups (LGs).
A set of 799 additional SNP markers was developed based on polymorphic sites identified in contigs and was used to anchor and orient metacontigs to LGs. This genetic map included 1,767 molecular markers arranged in 19 LGs covering 1,276 cM (Figure S2; http://genomics.research.iasma.it). The SNP-based markers were also helpful in merging the adjacent metacontigs not previously merged because of repetitive or low-quality links between them.
Integration of the DNA sequence and genetic map of LG4 is shown in Figure 1 (other LGs are in Figure S2). Table 2 summarizes the state of metacontig anchoring to the genetic map. The 2,093 metacontigs covered 477.1 Mb of genomic DNA. Of these, 435.1 Mb were anchored to the 19 LGs and 81.1% of these were oriented by two or more genetic markers (see Text S1). The smallest LG is covered by 26 metacontigs, the largest by 21 metacontigs. The order of markers established by meiotic recombination-based methods was almost co-linear with the metacontigs. In total, 82% of the genomic sequence was mapped to LGs. Most of the unmapped sequences were contained in 1,696 short metacontigs and singleton contigs with multiple tandem repetitive sequences. The assembly of metacontigs and facilitation of their placement on the genome using a genetic map avoided issues related to physical mapping.
V. vinifera genomic metacontigs (yellow bars) positioned along LG 4 of the Syrah x Pinot Noir genetic map. The map was assembled according to Cartwright et al. . On the left are marker names and positions, in centimorgans, from Troggio et al.  (http://genomics.research.iasma.it). Most metacontigs were anchored to the map using markers with unique sequence locations: SSRs, BAC-end sequences and SNP-based markers derived from either ESTs or assembled sequences of the two haplotypes of the Pinot Noir genome. Metacontigs without bridge markers were anchored based on their association to other metacontigs (details in Materials and Methods). Approximate size in Kb of each metacontig is indicated on the right. Gaps separating metacontigs are of undefined size.
Gene annotation and gene content
Five quality levels were adopted for transcript assignment (see Materials and Methods): i) transcripts confirmed by tentative consensus sequences (TCs) and gene predictions (8,110); ii) transcripts confirmed by TCs aligned to the genome (8,160) and among transcripts not confirmed by TC; iii) the retained transcripts predicted at the exon level by different methods (4,028); iv) transcripts which were positive in gene prediction methods with differences at the exon level but with correct gene boundaries (308); v) transcripts which were found by different methods with contrasting results: only genes encoding proteins with significant similarities to known proteins were accepted (8,979). In total 29,585 genes were predicted. Grape gene content is comparable to Arabidopsis (26,819) and markedly different compared with rice (41,046) and poplar (45,555) genomes.
Gene annotation followed a consensus approach. More than 79% of the genes predicted for the grape genome were annotated. Conserved putative grape genes were searched by the BLAST program with rice, poplar and Arabidopsis as references. A decision tree was implemented and used to carry this out. Sets of gene clusters with different levels of similarities among species as well as unique and putative species-specific genes were built. Using strict rules for homology determination, the subset of grape specific genes amounted to 16,859 (Figure 2).
All genes were compared each other as all-vs-all similarity searches using BLAST. Genes predicted for poplar, Arabidopsis and rice are respectively from www.genome.jgi-psf.org; www.arabidopsis.org; www.tigr.org. Grape gene estimates have been carried out on 58,611 assembled contigs. Genes of similar length with over 60% of similarity alignments at protein level were considered homologous using BLOSUM62 matrix . The frequencies of sequences shared among species are reported on the right.
Functional classification of the predicted genes was carried out by an automatic procedure. The manually revised final classification (Figure S3) shows the functional classes and their percentage in the gene set. Putative grape-specific genes were not characterized by a particular annotation profile or by relative abundance in the functional classes. A slight numerical difference in favour of grape was noted for genes related to lignin biosynthesis and to berry specific pectins. These metabolic pathways are less significant in Arabidopsis and poplar respectively. Genes relative to disease resistance and wine quality are discussed in further detail below.
Disease resistance genes
Resistance to parasites in plants is controlled by the non-host and gene-for-gene pathways . The non-host type was discovered only recently , . The gene-to-gene pathway is frequently present in cultivated plants displaying dominant resistance genes, responsible for the initiation of signal transduction leading to deployment of defense mechanisms . The majority of R proteins contain a nucleotide binding site (NBS) and a carboxy-terminal leucine-rich repeat (LRR) domain. The NBS is part of a conserved domain acting as a molecular switch for the signal transduction. The LRR is credited with recognition specificity akin to an antibody-like detector of pathogen effectors . At the N-terminus NBS-LRR proteins carry either the coiled coil (CC) domain or a domain homologous to the Toll/Interleukin-1 Receptor (TIR, ), allowing classification of NBS genes into two groups, the CC-NBS-LRR, present in all angiosperms, and the TIR-NBS-LRR, specific to dicotyledonous species .
Based on resistance domain analyses, the grape genome was found to contain 341 NBS genes (Figure 3 and Table S3), whereas 207 were found in Arabidopsis  and 398 in poplar . The 233 NBS genes which contain the LRR domain can be grouped in 5 major clades (1 to 5 in Figure 3A). The clades were comprised of CC-NBS-LRR, the dictot-specific TIR-NBS-LRR and their truncated structures as follows: (1) mainly TIR-NBS-LRR; (2) and (3) mainly CC-NBS-LRR; (4) mainly NBS-LRR; and (5) CC-NBS-LRR. The CC-NBS-LRR group included 84 genes in grape, 51 in Arabidopsis and 119 in poplar, while the TIR-NBS-LRR group included 37 genes in grape, 64 in poplar and 83 in Arabidopsis. In addition, the grape NBS gene family included 5 truncated TIR-NBS genes, 112 truncated NBS-LRR genes and 103 genes characterized only by the NBS domain (Table S3).
A) Phylogenetic analysis of NBS-LRR protein sequences of V. vinifera present in Pinot Noir. The phylogeny of these genes is based on a distance-matrix neighbour-joining analysis (Clustal X, ; bootstrap of 1000) after alignment of sequences by TCoffee (version 5.05, ). The phylogenetic clades, in general, correspond to the classification based on protein domains (however, see text and Table S3). B) Genes assigned to LGs are represented by dots. Their gene number is specified in LG-specific insets and in Table S3. NBS clades (see A above) contain mainly genes of the following classes: (1) TIR-NBS-LRR in blue; (2) CC-NBS-LRRa in green; (3) CC-NBS-LRRb in yellow; (4) NBS-LRR in cyan; (5) CC-NBS-LRR in red. Other resistance genes, belonging to NBS and TIR-NBS groups, are represented by the open and filled dots, respectively. Resistance-related genes different from NBS genes are shown in black. The size of each LG is given in Mb (on the right), whereas markers of the genetic map ( and http://genomics.research.iasma.it) are shown on the left, together with the interval in cM between the two closest markers in each gene cluster.
Besides NBS genes, the grape genome contains several signalling components of plant disease response which are encoded by genes EDS1, PAD4, COI1, MPK4, JAR1, ETR1 and NDR1, known to be recruited by resistance gene products (Table S3). The NPR1 gene, a regulator of the systemic acquired response to pathogens , is present in one copy in grape and in Arabidopsis, but has five copies in poplar. Likewise, RAR1 and EIN2 are present in single copies in the grape genome.
Genes encoding the pathogenesis-related proteins (PRs, ) include nine copies of PR-1, eight of PR-2, five of PR-3, one copy of PDF1, one of PDF2, and several copies of PR5 and protease inhibitor-like genes (Figure 3B and Table S3).
In addition, the grape genome contains eight genes similar to the MLO gene for mildew resistance in barley, compared to the 15 MLO-like genes known for Arabidopsis . MLO proteins belong to a large family of seven-transmembrane domain proteins specific to plants, encoded by genes homologous to barley MLO . MLO recessive alleles confer an effective resistance against mildew pathogens. Furthermore, the powdery mildew non-host resistance-related genes PEN1, PEN2 and PEN3 ,  were found in 5, 5 and 10 copies, respectively.
In grape, the disease-related genes represent a significant part of the genome. In spite of this, many grape varieties, including Pinot Noir, are susceptible to several fungi, such as grey mould (Botrytis cinerea), downy mildew  and powdery mildew , which have to be kept under control by heavy fungicide treatments. The failure to mount an effective defense response is probably due to a defective pathogen recognition. It is known that NBS-LRR genes are undergoing diversifying selection , e.g., variation in the sequence of the Arabidopsis gene RPS2 shows a signature consistent with pathogen-stimulated selection . Moreover, the extent of variation in the activity of NBS-LRR genes may have been affected by balancing selection –. Grape alleles of the same resistance genes did not co-evolve in the presence of the agriculturally most important grape pathogens . Indeed, allelic variation due to SNPs present in functional resistance domains was associated with the phenotypic divergence between resistant and susceptible genotypes only when susceptible V. vinifera and resistant non-vinifera clones were considered . In addition, the long time interval necessary for the grape to complete one generation, together with its vegetative propagation, makes it difficult to match the evolutionary rates of microbial or insect pests, which in vineyards are boosted by massive use of chemicals . Such detailed knowledge of the grape genome will serve to accelerate the development of genetic strategies to counter crop loss due to dynamic and genetically diverse pathogens.
The TIR-NBS-LRR genes are preferentially located in LG 18, the CC-NBS-LRR genes in LGs 9 and 13 and the truncated NBS genes in LGs 12 and 13 (Figure 3B). The NBS genes are also organized in clusters and superclusters. As noted in Arabidopsis , each cluster may include NBS genes of different phylogenetic lineages, although they frequently consist of tandem repeats of the same gene. The heterogeneity of NBS clusters has been discussed and interpreted as a consequence of evolutionary events such as ectopic recombination, chromosomal translocation and gene-cluster remobilization. This type of genome evolution is difficult to explain other than in terms of a hypothesis where a positive selection for cluster complexity provides the basic materials for the generation of new resistance specificities .
Several clusters of NBS genes mapped to chromosomal regions where genetic resistance to fungal diseases, such as downy and powdery mildew, were previously assigned (Figure 3B). This included LGs 12 and 18  and LGs 14 and 15 , , . Thus, the genome sequence of grape indicates candidate NBS genes responsible for extant variation and provides a starting point for breeding grape varieties resistant to important pathogens.
Phenolic and terpenoid pathways
Grape secondary metabolites, particularly polyphenols, have a strong influence on wine quality . Most phenolics derive from phenylalanine via phenylalanine ammonia-lyase (PAL). They encompass a range of structural classes and biological functions and include lignins, phenolic acids such as hydroxycinnamic and hydroxybenzoic acids, and polyphenols such as flavonoids and stilbenes.
Flavonoids are the most common plant phenolics. In flowers and fruits they attract pollinators and seed dispersers and are particularly involved in UV-scavenging and disease resistance . Flavonoids contribute to human health . The flavonoid skeleton, synthesized by chalcone synthase (CHS), is converted to chalcones, flavanones, flavonols, flavanols, anthocyanins and proanthocyanidins (condensed tannins). In red grape, flavanols and anthocyanins are abundant, the latter accumulating mostly in the berry skin and the former in the seeds . In the last decade considerable effort has been made in identifying and cloning grape flavonoid biosynthetic genes –. The grape genome sequence now offers the opportunity of compiling an exhaustive overview of the phenylpropanoid pathway.
Gene predictions corresponding to all those genes known to encode enzymes of the pathway could now be found. These include C4H and 4CL (acronyms are explicated in note 1 of Figure 4A) which were not previously identified in grape. The majority of genes were organized in large (PAL, F3'5'H) or small (CHS, F3H, FLS, LAR) gene families, the remainder consisting of single copy genes (C4H, 4CL, CHI, F3'H, DFR, LDOX, ANR, UFGT) (Figure 4A; Table S4).
A) V. vinifera general pathway for phenolics biosynthesis leading to stilbenes (C6-C2-C6) and flavonoids (C6-C3-C6). For each enzyme, the gene copy number is reported in brackets. Genes were identified by similarity search using BLAST where the references were the sequences of phenolic biosynthetic genes previously characterized in grape and in other plant species. Putative homologues and gene copy numbers were determined by comparing aligned amino acid sequences based on a threshold of 80% similarity between the grape sequences, and 60% similarity between grape and other species. For the large StSy, PAL and F3'5'H families, phylogenetic analysis was performed with MEGA4 package  after aligning with ClustalW . The following enzymes involved in the pathway are shown: PAL, phenylalanine ammonia-lyase; C4H, cinnamate 4-hydroxylase; 4CL, 4-coumarate-CoA ligase; CHS, chalcone synthase; StSy, stilbene synthase; RSGT, resveratrol glucosyltransferase; CHI, chalcone isomerase; F3H, flavanone 3-hydroxylase; F3'H, flavonoid 3′-hydroxylase; F3'5'H, flavonoid 3′,5′-hydroxylase; DFR, dihydroflavonol-4-reductase; FLS, flavonol synthase; LDOX, leucoanthocyanidin dioxygenase; LAR, leucoanthocyanidin reductase; ANR, anthocyanidin reductase; UFGT, UDP-glucose:flavonoid 3-O-glucosyltransferase; OMT, O-methyltransferase; ACCase, acetyl CoA carboxylase. PA refers to proanthocyanidins. Enzymatic steps that have not been experimentally confirmed are marked with an asterisk (*). B) Steps of plastidic isoprenoid pathway and monoterpenoids biosynthesis. For each enzyme, the gene copy number is reported in brackets. Gene annotation was performed as described in Material and methods. Abbreviations: G3P, glyceraldehyde 3-phosphate; DXP, 1-deoxy-D-xylulose-5-phosphate; MEP, 2-C-methyl-D-erythritol 4-phosphate; CDP-ME, 4-diphosphocytidyl-2C-methyl-D-erythritol; CDP-MEP, 4-diphosphocytidyl-2Cmethyl-D-erythritol 2-phosphate; ME-cPP, 2C-ethyl-erythritol 2,4-cyclodiphosphate; HMBPP, 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate; IPP, isopentenyl pyrophosphate; DMAPP, dimethylallyl pyrophosphate. The enzymes in the pathway are indicated in blue: DXS, 1-deoxy-D-xylulose 5-phosphate synthase; DXR 1-deoxy-D-xylulose 5-phosphate reductase; ISPD, 4-diphosphocytidyl-2-C-methyl-D-erythritol synthase; ISPE, 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase; ISPF, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; ISPG, 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase and ISPH 2-C-methyl-D-erythritol 2,4-cyclodiphosphate reductase (ISPG and ISPH are probably the same enzyme and convert directly MEcPP in IPP and DMAPP); ISPA, geranyltransferase; IDI, isopentenyl diphosphate delta-isomerase; PT, prenyltransferase; LIMS, limonene synthase; LIS, linalool synthase; GES, geraniol synthase; TES, α-terpineol synthase; TPS-CIN, myrcene/(E)-beta-ocimene synthase; CYTP450, cytochrome P450 hydroxylase; ALDH, aldehyde dehydrogenase; NADPDH, NADP dehydrogenase. Enzymatic steps that have not been experimentally confirmed are marked with an asterisk (*).
Within the phenylpropanoid pathway, relatively large gene families have been described for poplar compared to Arabidopsis . Our results highlight some significant differences, such as the number of PAL and F3'5'H gene copies which were even greater in grape. In general, grape and poplar secondary metabolism exhibits a tendency toward gene family expansion. Conversely, in Arabidopsis all enzymes of the central flavonoid metabolism, except for FLS, are encoded by single genes . This is consistent with the noted low metabolic investment in flavonoids of Arabidopsis, a species which reproduces without the need for insect pollination and has no perennial woody habit.
In grape, as in a few other species, the condensation of p-coumaroyl-CoA with malonyl-CoA gives rise to stilbenes via stilbene synthase (StSy; ). Among stilbenes, monomers and oligomers (viniferins) of resveratrol contribute to resistance to fungal pathogens . Resveratrol has gained attention due to its alleged beneficial effects on human health . Stilbene synthase belongs to a large family: the analysis of the grape genome predicts at least 21 copies. This number agrees well with a recent StSy sequence analysis in infected grape leaves  but it differs from the one predicted in the PN40024 grape genome sequence . Most of these copies, as well as most PAL genes, are clustered in LG 16. Further, several peroxidase genes were predicted, some of which could participate in the formation of viniferins, as previously suggested . Recently, a resveratrol glucosyltransferase putatively involved in piceid synthesis has been isolated and biochemically characterized in V. labrusca grape berry . Our analysis revealed that its homolog in Pinot Noir (99% sequence similarity) is present as a single gene mapping on LG 3.
Terpenoids are among the most abundant and structurally diverse group of natural metabolites. Volatile and non-volatile terpenes are essential for plant growth and development (e.g., gibberellin phytohormones), but they are also key players in the interaction of plants with the environment . The substrates for the biosynthesis of about 22,000 terpenes are isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). The mevalonate (MVA) and the mevalonate-independent DOXP/MEP pathways are responsible for the synthesis of IPP and DMAPP in the cytosolic and plastidic compartments respectively . DOXP/MEP is the dominant route for monoterpene biosynthesis in the grape berry . Three prenyltransferases produce terpene precursors, prenyl diphosphates, geranyl diphosphate (GPP), farnesyl diphosphate (FPP) and geranylgeranyl diphosphate (GGPP). Terpene synthases (TPS) catalyze the formation of hemiterpenes , monoterpenes (C10), sesquiterpenes (C15) or diterpenes (C20) from the substrates DMAPP, GPP, FPP or GGPP respectively (Figure 4B).
All TPSs are similar in physico-chemical properties. Moreover, the close sequence relatedness of their genes prevents discrimination of their catalytic functions, supporting a rapid divergence of catalytic activity of closely related TPS genes . Three classes of TPSs are described and only classes II and III are specific for the plant secondary metabolism . Forty seven TPS genes participate in the secondary metabolism in poplar , while in grape only 35 TPSs were identified, a number close to the 32 found in Arabidopsis. In the grape genome, they are located mainly on LGs 9, 10 and 19 (class I TPs on LGs 7, 9, 10 and 19, Table S5).
Several higher plant genes of the terpenoid pathways have been cloned , but only a few of them had previously been identified in grape . Having the complete sequence of the grape genome, 124 genes related to the terpenoid pathway were identified (Table S5). Of these, 110 were mapped to all LGs. Functionally, 24 are related to carotenoids, 24 to abscisic acid metabolism, 10 to gibberellin hormones, and 6 cover steps of the core terpenoids pathway: 5 prenyltransferases and 1 isopentenyl diphosphate delta-isomerase. For the MVA and non-MVA pathways, nine (4 DXS, DXR, ISPD, ISPE, ISPF, ISPH) and eight (2 AACT, HMGS, 3 HMGR, MK/MVK, MVD) putative genes were identified respectively.
Plant monoterpens are preferentially confined to specialized organs. They play an important role in defense as well as acting as allelopathic agents and attractants for pollinators . In grape, monoterpenes contribute to wine free volatiles: typical components of the aroma-rich grape varieties are linalool, geraniol, nerol, citronellol and α-terpineol, which are stored in exocarps and vacuoles. Monoterpene biosynthesis has not yet been studied because several metabolic steps may take place without enzymatic catalysis. Moreover, the knowledge of mechanisms controlling monoterpene synthase activity is still largely incomplete. In the grape genome four monoterpene synthase genes were identified encoding linalool synthase, limonene synthase, myrcene synthase and α-terpineol synthase.
In grape, 2004 TF genes were identified (Figure 5A and Table S6) which represent 6.7 % of the genome, similar to the 6% for Arabidopsis , 4.8 % for rice  and 6% for poplar . Among the grape TF genes, 80.6% are present in marker-anchored metacontigs (Figure 5A).
A) Distribution of 1,617 transcription factors along the 19 V. vinifera LGs inferred from the positions of anchored metacontigs. Different colours of the histograms corresponds to the different TF classes. B) Distribution of transcription factor clusters over the grape genome. TF organization in LGs 2, 5, 6, 7, 8, 10, 13, 16, 17 is presented. For each LG, markers of the genetic map, developed by Troggio et al.  (see also http://genomics.research.iasma.it) are reported on the left together with the interval in cM between the two closest markers for each TF cluster. TF types are reported on the right.
Sixty-two families of TF genes were found, a number similar to the 64 for Arabidopsis, 62 for rice and 63 for poplar . TF families like MYB, AP2/EREBP, bHLH and MADS-box include a large number of members , . We compared the number of genes in each of the 60 grape TF families in common to the other three plant genomes: finding a nearly linear correlation (Figure 6). Thus the organization and number of TFs seem to be highly conserved in plant genomes. TF distribution in the grape genome (Figure 5A) indicates that only LGs 7 and 18 have a higher than average TF content. Clusters of AP2/EREBP genes are repeated in tandem on LGs 2, 7, 10 and 16; CCAAT genes on LGs 6, 8, 10 and 13; MADS-box genes on LGs 5 and 13; Myb genes on LG 8 and 17 (Figure 5B).
For each of the 60 families (1983 genes) of V. vinifera TFs (X-axis) (log base 2 transformed), family members have been plotted against the corresponding number reported for three other genomes: A) A. thaliana (http://arabtfdb.bio.uni-potsdam.de/v1.1), B) P. trichocarpa (http://poplartfdb.bio.uni-potsdam.de/v2.0) and C) O. sativa (http://ricetfdb.bio.uni-potsdam.de/v2.i). The degree of the correlation among TF gene numbers is indicated by the Pearson correlation value (r). Each scatter plot shows the TF families which were statistically over- or under- represented in pair-wise comparisons (χ2 tests were applied to untransformed data; p = 0.05).
Across the species mentioned, MYB (279) are the most abundant , . They play a role in controlling the accumulation of secondary metabolites in the grape berry –. A gene from this TF family is also known to play a key role in the regulation of anthocyanins and flavonols during the non-climacteric ripening of strawberry . Non-climacteric ripening (occurring in fruit such as strawberry and grapevine) is a process characterized by the absence in respiratory pick and ethylene bursts, two phenomena typical of the climacteric fruits ripening.
In the grape genome were also found 143 leucine-zipper genes. Together with EREBP TFs they contribute to the plant's defense response . In tepary and common bean, a bZIP gene plays an important role in the response to water deficit and in the regulation of abscisic acid levels. 
In the grape genome, the MADS-box family is also over-represented. These TFs regulate flowering-related phenomena, as well as other metabolisms . MADS-box TFs may have been important during plant evolution because they allow plant reproductive structures to adapt to variations in climatic conditions . It was found that two tandem MADS-box genes (MADS-RIN and MADS-MC) regulate fruit ripening and inflorescence determinancy in the climacteric fruit tomato. Mutation at rin locus caused a failure in the normal ripening physiology .
A ripening mechanism common to both climacteric and non-climacteric species, such as grape, has been hypothesized . In support of this, we identified two TF classes in grape, AP2/EREBP and EIL, which contribute to ethylene signalling during ripening of climacteric fruits, and also found ethylene receptors belonging to ETR/ERS families.
Matching the sequences of assembled contigs with original reads made it possible to characterize each DNA segment by the number of matching reads (see Materials and methods). For the read coverage of 10.7X, a DNA segment was considered unique when represented by 15 or fewer matches. Moderately repeated sequences (2 to 8 copies per genome) were expected to have 16–100 matches. Sequences with more than 100 matches were considered highly repetitive. They were masked before gene prediction, thus excluding most of the coding parts of repetitive elements from the putative gene set.
Dispersed highly repetitive DNA sequences were identified by an iterative procedure, and the resulting collection of 90,483 repetitive segments were grouped into 136 types. Members of each type were translated and compared to each other and the similarity scores were used in a UPGMA-like clustering. The similarity tree consisted of eight clusters lacking a common root (Figure S4), each of which was assigned to the known classes of repetitive DNA sequences (Table S7).
Grape transposable elements (TEs), totalling 108.5 Mb, represent the most abundant set of repeats. The repeats were included in group I (retrotransposons: Copia, Gypsy, LINE) and group II (DNA transposons: Mutator, CACTA, hAT) according to Feschotte et al. . The most abundant TEs were Gypsy/athila-like elements followed by Copia elements. DNA transposons were represented by 9,562 copies (7.1 Mb). The TEs seem to be more abundant in grape compared to poplar , Arabidopsis and rice . Putatively autonomous TEs were identified by significant BLAST analysis against the Uniprot database. TEs without a significant BLAST hit were attributed to the non-autonomous group (Table S7). Out of 136 repeat types, 20 were classified as long tandem repeats with a unit size from 100 to 430 bp. They were grouped into ten major sub-classes.
Short tandem repeats (microsatellites) were also identified. Their thresholds, number of copies and total DNA length are reported in Table S8. Microsatellites cover 2.1 Mb, including the telomeric repeats (TTTAGGG). Out of 171 contigs with identified telomeric sequences, 42 had telomeric ends. In the linkage map, they represent potential markers for telomeres.
An alternative estimation of the length of identified repetitive DNA was performed using the number and total length of reads matching repeat sequences, identified above. This new estimate gave a value of 138.5 Mb, corresponding to 27.4% of the 504.6 Mb genome size.
MicroRNAs (miRNAs) and trans-acting siRNAs (ta-siRNAs) have a significant role in plant development and stress response , . The majority of the 1220 plant miRNAs listed in the miRBAse  are from Arabidopsis (184), rice (243) and poplar (215). A BLAST search of sequences similar to the Arabidopsis miRNAs genes was performed on the grape genome. Allowing for three or fewer mismatches, 143 miRNA genes representing 28 families (; Table 3, Table S9) were identified.
Three types of miRNAs (miR827, miR828 and miR846) were not previously found outside Arabidopsis, and were considered “non-conserved” miRNAs , . However, these genes are present in the grape genome, indicating that they were either lost in the lineage leading to Populus or are missing from its genome assembly. The miRNA passenger strands (miRNAs*) are highly conserved between grape and Arabidopsis . Sequences predicted to produce ta-siRNAs  are conserved in several plant species, grape included.
Putative grape miRNAs and siRNAs target the same classes of genes as they do in Arabidopsis, rice and poplar: transcription factor genes, genes involved in stress response and nutrient uptake, genes for RNA silencing and the non coding RNA TAS3 (Table 3). In grape, 56 RNA-dependent DNA polymerase genes are potentially targeted by miR396 and miR846, a phenomenon not reported in other plant species.
BLAST searches identified four Dicer-like proteins (Helicase, RNAse IIIa/b domains), nine Argonautes (PAZ/PIWI domains), and six RNA-dependent RNA polymerases (RdRp domains), indicating the presence in the grape genome of a complex RNA processing machinery (Figure S5).
The tRNAscan-SE program  identified 719 putative tRNA genes. 163 of them are pseudogenes, 3 are suppressors for the TAA codon while 553 correspond to 52 anticodons for all amino acids (Table S10).
Small nuclear RNA.
Non-coding RNAs include five major and four minor snRNA families, all components of splicing factors. The Arabidopsis snRNA list of Wang and Brendel  was used to search for similar sequences in grape. We found 89 snRNA genes and pseudogenes (75 in Arabidopsis) (Table S11). Several snRNA genes were clustered in the genome.
Large rRNA units consist of two segments, one hosting the genes 18S rRNA, 5.8S and 28S, the second containing three arrays of tandem repeats. In grape the length of the rRNA unit is around 10.8 Kb. The variable segment includes three arrays of tandem repeats: about 40 copies of a 44–45 bp repeat, three copies of a 150 bp repeat and 5.5 copies of a 193 bp repeat. The unit is repeated 1450–1550 times in the genome (16.1 Mb). rRNA units may contain insertions of retrotransposons of three different lengths (2870, 2950, and 5800 bp). Retroelements in rRNA sequences may cause transposition of rRNA sequences.
The DNA sequence for the small ribosomal RNA unit (1,250 bp) contains two genes for 5S rRNA, 120 bp each with a single nucleotide difference between them. In the genome the unit was represented by 170–180 copies. Together, large rRNA and 5S rRNA sequences were estimated to amount to 16.3 Mb.
Small nucleolar RNA.
Based on the Arabidopsis snoRNA genes , 166 sequences representing 79 families were found in grape (Table S12). Most of the grape snoRNA genes (110) are clustered; 62 snoRNA genes were located inside 34 genes encoding six ribosomal proteins and one eIF-4F factor.
Pinot Noir genome structure and evolution
The existence of structural diversity between homologous chromosomes within plant species has been reported . This type of molecular variation seems to be common in allogamous plants  and could also be a characteristic of autogamous species . Grape does not tolerate long term inbreeding  and high outcrossing rates maintain the genome in a heterozygous state, as evident in the remarkable variation found in collections of grape varieties .
The genome sequence data from a cultivated grape variety provides unprecedented insight into the structural nature of heterozygosity in an outcrossing species. The variation within this clone of grape consists largely of chromosome-specific gaps and hemizygous DNA. In addition to the regions in which it was possible to merge haplotypes representing DNA from both chromosomes in a consensus sequence, regions were found which were chromosome-specific, i.e., either with different DNA sequence flanked by orthologous regions of the two homologous chromosomes (hemizygous DNA) or gaps corresponding to sequences absent in one chromosome but not in the other. One million gaps, covering 48.9 Mb, and 65.1 Mp corresponding to hemizygous DNA distributed in 22,610 contigs were identified. These data allow us to conclude that the homologous chromosomes of Pinot Noir differ on average by 11.2 % of their DNA sequences and that the grape genome exists in a dynamic state, mediated at least in part by transposable element activity, as reported for helitron TE . Indeed, the large grape genomic gaps are frequently bordered by 5 bp direct repeats, reminiscent of a type of DNA excision mediated by a precise process of transposition .
The genomic region represented in Figure 7A highlights the differences which exist between homologous haplotypes. Notable differences in this region concern the presence of gaps and the number of copies of TE.
A) Comparison of constrasting haplotypes (a and b) co-mapping at two almost contigous regions in metacontig 32,921 of chromosome 1. Above: the 188 kb region; below: the 215 kb region. I from contig groups 1030-H15, 1079-G03, 2068-K04, 1034-C17 and II 2010-J07, 2044-L11, 1030-N10. In the genetic map the two regions are positioned at 60.1 cM: see preliminary experiment in Text S1. TE elements are labeled as follows: c: Copia; g: Gypsy/gypsy; a: Gypsy/athila; d: hAT/Dart; k: Karma; h: hAT; m: Mutator. B) SNP profiles of the 19 LGs of V. vinifera. Left and right of the figure correspond respectively to top and bottom of LGs of Troggio et al. . The SNP values reported do not consider gaps in and among metacontigs. C) SNPs in exons and non-coding DNA and percentage of anchored genes tagged with SNPs. In parts B to E of this figure, gene prediction and annotation and the exon-intron boundaries were based on the methods described in Solovyev et al. ; Korf et al. ; Majoros et al. ; Altschul et al. ; Huang and Madan . D) Relative age of grape duplicated genes estimated from the number of synonymous substitutions per synonymous sites (KS values). The peak between 0.6 and 1.2 KS supports a relatively large scale duplication event. Paralog genes were identified as in Li et al.  and KS distributions were calculated as in Maere et al. . E) The same as in D for genes present in duplicated chromosome segments.
In the preliminary experiment (see Text S1), it was found that the frequency of SNPs correlated with deletions and insertions. Segments with less than one in/del per Kb had 4.4 SNPs per Kb, whereas segments with one or more in/del per Kb had 16.7 SNPs per Kb. A total of 2 millions SNPs (1,751,176 anchored and the remaining present in other assembled sequences) were discovered and validated and more than a million in/dels were annotated on the sequence with defined location. Our data allow us to extend the evaluation of nucleotide variation to the entire genome rather than to limited resequenced DNA regions . Among recently sequenced animal genomes, a high SNP frequency was found in sea urchin  and Cyona intestinalis . Across the grape genetic map (Figure 7B), the SNP frequency had an average value of 4.0 per Kb.
Coding and non-coding regions demonstrated different degrees of polymorphism with 2.5 and 5.5 SNPs per Kb respectively. One or more SNPs were found in 86.7% of anchored genes and 71.4% of genes had more than four SNPs (Figure 7C). Those gene-based markers are valuable tools, as SNPs present in functional genes may cause natural phenotypic variation ,  and help in genetic diagnosis. In addition, we noticed some reduction of SNP frequency in gene desert regions, described for the dog genome .
In several regions of the 19 LGs, SNP frequency peaks between 5 and 7.5 per 1 Kb, even if the frequency may reach values much higher than those cited (Figure 7B). Other regions displayed dramatically reduced frequencies. Therefore, as shown for human , dog  and Anopheles  genomes, the Pinot Noir chromosomes consist of large blocks where two haplotypes are present. The sparseness of putative quasi-homozygous haplotypic blocks indicates that heterozygosity prevails.
Arabidopsis and poplar have likely undergone three rounds of whole genome duplications during evolution , , , although this has been challenged recently . The first duplication (referred to as 1R, , ) may have predated the divergence of monocots and eudicots, while the second one (2R) probably occurred around the radiation of the core-eudicots prior to the divergence of poplar and Arabidopsis , . The most recent duplications in poplar and Arabidopsis have occurred after their divergence . The current thinking is that Vitis is an early diverging lineage within the rosids that has diverged prior to the divergence of Arabidopsis and poplar . We determined the relative age of grape duplicated genes from the number of synonymous substitutions per synonymous site (KS). The age distribution of Vitis duplicates shows a clear peak of KS values between 0.6 and 1.2 suggesting a relatively recent large-scale duplication event (Figure 7D). A smaller peak is also visible for KS values between 2.0 and 2.5, probably corresponding to more ancient large-scale duplications, as is the case for poplar and Arabidopsis .
Different approaches were taken to estimate the age of the youngest large-scale duplication event. First, it should be noted that the youngest peak lies to the left of the peak formed by KS values between orthologs of Vitis and Arabidopsis (Figure S6) although one should be very cautious in comparing different KS distributions due to different substitution rates in different organisms. Second, we also detected duplicated segments, covering about half of the genome, using a previously described method . KS values of genes in these duplicated blocks (Figure 7E) showed that the majority of these are responsible for the 0.6–1.2 KS peak (Figure 7D) and thus likely to be remnants of a single large-scale duplication event. We have also used phylogenetic approaches (see Methods) to estimate the relative age of genes in duplicated blocks. In total, 485 gene pairs support duplication prior to the split Arabidopsis–Vitis, while 523 gene pairs support duplication after the divergence of Arabidopsis and Vitis, i.e., are Vitis specific, although distributions of KS values for these two sets of genes are not discernable (not shown). When duplicated blocks of which at least two-thirds of the anchors support the same tree topology are considered, almost twice as many blocks support duplication within the Vitis lineage than before the divergence of Vitis. As a matter of fact, we suspect the actual number of genes supporting a Vitis-specific duplication to be higher. Indeed, it has been shown in several studies that, following gene duplication, one of the duplicates evolves at an increased rate , . This could easily lead to the inference of erroneous tree topologies where one of the Vitis duplicates branches off earlier than it should, in particular if the duplication event occurred shortly after the specialtion of Vitis (see further).
Jaillon et al.  propose that three ancestral genomes contributed to the Vitis lineage and suggest ancestral hexaploidization for most eudicots, while not finding evidence for a recent duplication in grape. Furthermore, they suggest that, since their split, poplar has undergone an additional whole genome duplication, while Arabidopsis has undergone two additional genome duplications. These results are at odds with our findings. Reanalysis of Arabidopsis and poplar genomes (not shown) uncovers, for both, many homologous segments with a multiplication level between five and eight, which suggests three rounds of duplications for both genomes . If the Arabidopsis and poplar genomes were ancient hexaploids, to which two additional genome duplications had been added, fragment multiplication of up to twelve should be expected for Arabidopsis, and up to six in poplar.
The fact that there is substantial ambiguity in the dating of the duplicates in duplicated segments suggests that the most recent large-scale duplication event reported here for Vitis might have occurred in close proximity to the Vitis speciation event. Therefore, an alternative scenario than the one presented by Jaillon et al.  that we would like to put forward is shown in Figure 8. We assume three genome duplications to have occurred in both poplar and Arabidopsis, as proposed earlier , , , one of which has been shared by all dicots (and possible also by the monocots, see ), one that has been shared by Arabidopsis and poplar, but not Vitis, and one that has been specific to Arabidopsis ,  and poplar , respectively. Since many regions of the Vitis genome appear in triplicate in both Jaillon et al.  and our own analyses (not shown), the genome duplication shared by all dicots might have been followed by a hybridization event in Vitis, shortly after its divergence from the lineage leading to poplar and Arabidopsis (see Figure 8).
Alternative scenario to the one proposed by Jaillon et al.  to explain angiosperm genome evolution. Our analyses seem to suggest that there has been a large-scale duplication event, likely a hybridization event, in the Vitis lineage, rather than before the split of Vitis and other dicots. See text for details.
The Grapevine Genome Initiative was established with the aim of accelerating the breeding of a difficult perennial species. Grape breeding for disease resistance, if not for immunity, would be a solution to the problem of the emergence of aggressive races of micro-organisms that are currently controlled by massive use of agrochemicals. The problem is not a simple one: how to modify a complex and highly heterozygous genome without altering wine quality. Precise knowledge of all the genes influencing quality and resistance traits is an absolute prerequisite for such modifications.
A high number of genes related to disease–resistance have been identified; many of them have been mapped to LGs and a large part of them are tagged with one or more SNPs. These resistance genes, however, did not co-evolve in the presence of the most important grape pathogens , a condition which may have not sufficiently protected the species. This is in part the reason why a deep knowledge of the grape genome is the starting point for developing genetic strategies to counter pathogens.
Description of the grape genome sequence opens the opportunity for molecular breeding in grape. The fertility of hybrids between wild and domesticated grape species with 19 seemingly co-linear chromosomes , – makes it feasible to introduce new resistance genes via traditional breeding. The NBS gene clusters identified here can be associated with QTLs affecting disease resistance or tolerance behaviour of grape varieties (this is the case with LGs 12, 14, 15 and 18; , ). This large and underexploited reservoir of resistance genes could be easily moved in clusters across genomes by choosing appropriate molecular markers to selectively introgress only the resistance traits. This would prevent the loss of alleles important for grape and wine quality. Thus, the anchored sequence of the grape genome, together with the large arsenal of SNP loci, now offers a tool to open a new era in the molecular breeding of grape.
WGS using longer read dye-terminator sequences can be combined with shorter SBS sequence data using dedicated assembly programs. Using this method we have resolved a complex heterozygous eukaryotic genome. Future whole genome sequencing efforts should be able to combine these two methods to produce assemblies in shorter times while reducing the need for resources. The ability to resolve the haplotypes in Pinot Noir suggests that sequencing DNA mixtures, for example more than one genotype of a given crop, is practical. Such an approach generates both a consensus sequence of the genome and a set of mapped marker loci to be used in breeding programs.
Materials and Methods
In order to prepare shotgun libraries, DNA was extracted from young shoots of Pinot Noir, clone ENTAV115, randomly sheared and size-selected. Two BAC libraries were also constructed (; Keygene, Wageningen, NL) and clones assembled in a physical map (http://genomics.research.iasma.it). A population of 94 F1 plants from the cross between Syrah and Pinot Noir was the source of the DNA used for mapping markers and anchoring metacontigs.
Fosmid and shotgun libraries were from DNA purified by a CTAB method . Sheared DNA (Gene Machines Hydroshear, Ann Arbor, MI) was size selected to produce libraries with insert sizes of 2, 3, 6, 10 and 12 Kb. DNA was ligated to a high copy plasmid vector and transformed into DH10B T1r E.coli cells (Invitrogen, Carlsbad, CA). The fosmid library was produced from DNA fragments between 30 and 45 Kb. DNA inserts were ligated into a pCC1FOS vector packaged with MaxPlax lambda extracts and transfected into EPI300-T1r E.coli cells (Epicentre, Madison, WI). LB agar contained chloramphenicol and 99,840 clones were picked (QPix2 Genetix, Hampshire, UK) into 384 well plates containing LB freezing medium, incubated for 18 h, replicated and stored at −80°C.
Sanger shotgun sequencing
DNA was amplified from bacterial cultures by a rolling circle technology (Templiphi kit; GE Healthcare, Amersham) and Sanger sequenced on MegaBACE 4500. Clones with inserts from 6 to 20 Kb, BAC clones and clones from fosmid libraries were amplified by the Templiphi large kit. BAC clones were bidirectional dye terminator sequenced on ABI Prism® 3730.
Sequencing by synthesis (SBS)
Pinot Noir DNA isolated as described was subjected to nebulization to generate fragments of approximately 620 bp. These were amplified as in Margulies et al.  and sequenced on the Genome Sequencer 20 (Roche Applied Sciences, Indianapolis, IN). The standard protocols for 454 Sequencing using the Genome Sequencer 20 system call for the generation of a library of tagged single stranded DNA molecules (see Margulies et al  for details). This single stranded library is then tested for optimal sequencing parameter through generation of sequencing beads by emulsion PCR with dilutions of the single stranded library. This titration step determined that three microlites of a single stranded library were used to generate 23 million beads. The standard GS20 pyrosequencing profile uses a sequencial flow of each nucleotide in a repeating pattern of TACG. This pattern is repeated for 42 cycles as per the standard protocol and generates 100bp of sequence information on average. For the purposes of generating longer sequencing reads the sequence profile of 42 cycles of nucleotide flows was changed to 100 cycles which increased the average read length from 105 bp to 200 bp. The GS20 has standard software to recognize high quality reads and convert the signal (light) into a base call. The standard software GS20 package was used to generate the sequence files. In total, 12.5 million reads corresponding to 2,111 million Q20 bases were produced.
Clones bridging neighboring contigs were selected for gap closure. The clones were grown in 384-well plates and sequence-specific primers were designed and used in dye terminator sequencing reactions resolved on MegaBACE 4500.
6.2 million reads for a total of 3.5 billion Q20 bases were produced by Sanger sequencing from 43 libraries (Table S1) and about 90.6% of reads were paired. Chloroplast sequences were detected and the chloroplast genome was assembled for assessing the sequence quality and insert size distribution of each library, characteristics that were used in assembly. Chloroplast forward and reverse reads validated the correctness of data tracking and the contamination level for each sequencing plate. The size of the chloroplast genome was 160,928 bp. Remarkably, the sequence was identical (without a single mismatch) to the one already published .
SBS data were essential to identify polymorphic sites and close small gaps. The amount of chloroplast and mitochondrial sequences in SBS data was 5.5 and 2.0%, respectively, vs 3.1 and 1.8% in Sanger sequences. Four programs developed at Myriad Genetics Inc. were organized into a pipeline for WGS assembly: (1) Sanger and SBS sequences were compared by the Match program. It produced a table of pairwise sequence overlaps with indication of the sequence orientation, offset and match score. The overlaps were accepted if they involved more than 50 bp with no more than 2% of polymorphic positions. (2) Consensus sequences were built using the Assemble program, adapted to specified levels of heterozygosity (2% or less) and large gaps (up to 500 bp). The program reads the sequence and quality data in Fasta or GDE format, considers clone sizes and performs multiple alignments, building the consensus sequence and reporting polymorphisms of the sequence. (3) Sequences were aligned with the Align program in a two-step procedure including fast search of identical segments and optimal alignment of gaps up to 7 Kb. Larger or multiple gaps may still be a problem for the alignment and leave some overlapping contigs not merged. (4) Visual comparison of two sequences was performed by the Dotmap program. The result of the assembly is a Fasta file of assembled contig sequences with quality values assigned for each position and the list of positions of polymorphisms. (5) Metacontigs were constructed as ordered and oriented groups of contigs linked with paired reads matching to non-repetitive parts of the contigs. We used also marker information to avoid building chimeric metacontigs from different LGs (see Text S1 for more details).
Genetic maps and genome integration
Metacontigs were integrated in the 19 grape LGs based on the genetic map derived from the cross Syrah X Pinot Noir. To improve marker density, polymorphic sites identified during WGS were selected for developing 799 additional SNP-based markers (http://genomics.research.iasma.it) using the SNPlex™ Genotyping System . DNA was prepared according to the instructions and the samples were analyzed on the ABI PRISM® 3730xl (Applied Biosystems, Foster City, CA). Data were analyzed by Gene Mapper v. 4.0 (Applied Biosystems, Foster City, CA). The genetic maps were followed a double pseudo-testcross strategy . Marker phase was determined by the Phasing algorithm (http://math.berkeley.edu/dustin/tmap/; ), which provides LG assignment and ordering of loci. LG were assembled with a minimum LOD of 8.0 and a maximum distance of 35 cM. Homologous LGs of the two parents were merged in a consensus map.
Genes and gene families
Methods used were FgenesH , homology-based FgenesH+ , Twinscan , GlimmerHMM  and Tentative Consensus  transcripts derived from 320,000 ESTs deposited in databases. Trimmed sequences were clustered using MegaBLAST  and aligned using Cap3 . After quality testing 28,856 TCs were retained.
BLAST searches against Uniprot and plant protein databases, annotated with GO terms, of various domain libraries were the base for gene annotations GO terms were extracted from BLAST searches against KEGG databases, KOBAS of metabolic pathways and InterproScan  and clustered using their semantic similarity , accuracy weight and the path from the root node of the ontology to the most detailed annotation. More than 79% of the gene models were annotated.
Functional classification was based on Gene Ontology (www.geneontology.org) and manually controlled.
Homologs across species were established using a BLAST search against Rice, Poplar and Arabidopsis, considering sequence alignment coverage, best multi directional BLAST hits, sequence identity and protein domains. Sets of clusters reflected different levels of similarity among species as well as unique and putative species-specific genes. For the analysis of specific gene families, methodological variations were introduced as reported in text.
Genes with similarities to TEs were removed and paralogs identified as in Li et al. . Age distributions were build as described by Maere et al . Duplicated segments were analyzed with i-ADHoRe , based on the following parameters: gap size of 40 genes, Q value of 0.9, probability cut off of 0.001, and a minimum of 3 homologs to define a duplicated segment.
Phylogenetic trees for duplicated genes (so-called anchors) in duplicated segments were based on pairs of grape paralogs representing the reciprocal best hits with aligned length of >150 amino acids and considering comparisons with proteins from Physcomitrella patens, used as outgroup, and the best Arabidopsis homolog. Proteins were aligned with CLUSTALW and only unambiguously aligned regions were considered. Tree construction used seqbot, protdist, neighbour and consense from the PHYLIP package  with 1000 replicates. Only topologies with over 70% bootstrap support were considered. For each paralog, if the topology was (Grape1, Grape2) Arabidopsis, it was concluded that the paralog was duplicated after the split of grape and Arabidopsis.
Based on 10.7X coverage, a DNA segment was defined unique when associated to 15 or less matches. The threshold was selected as the middle point between two Poisson distributions, with 10X and 20X the expected coverages corresponding to unique and duplicated segments, respectively. For dispersed repetitive sequences, an iterative procedure was developed. Each segment was searched against all sequences, starting with the repeat presenting the highest number of matches. At each iteration, the program identified repeats with decreasing similarity to the original seed repeat, and the complete set of copies of a particular repeat cluster was obtained. These DNA segments were masked and the remaining sequences were searched for the next repeat with the highest number of matches. Members of each of the identified repeat types were translated and compared using BLAST program. The similarity scores were used in a UPGMA-like clusterization. Short tandem repeat (microsatellite) motifs were identified by a specifically designed program considering their number above a threshold. This was selected based on the occurrence of the motif in the genome so that the number of segments with units exceeding the threshold would be less than 1.
Methods used for miRNA detection and individuation are cited in the caption of Figure S5. Methods and reference papers for tRNA, snRNA and snoRNA are cited in the text. Ribosomal RNA were defined and computed according to assembly program of Myriad Genetics Inc. (Salt Lake City, Utah).
The reference information was from PlnTFDB, an integrate plant transcription factor database  including genes from A. thaliana (ArabTFDB), P. trichocarpa (PoplarTFDB) and O. sativa (Rice TFDB) (available at http://plntfdb.bio.uni-potsdam.de). For each TF family, conserved domains were used as queries for searching similar sequences in the grape genome. The protein domains of identified TF were classified using the Pfam database .
Supporting text; supporting references
(0.04 MB DOC)
Histograms of contig size distribution. Histograms showing the distribution of the assembled contigs in size classes. The average contig size is 9.1 Kb. Half of the genome is covered by 7,878 contigs larger than 18.2 Kb.
(0.08 MB TIF)
Anchored and oriented metacontigs along the 19 LGs. Representation of the 435.1 Mb of V. vinifera genomic sequence contained in 397 metacontigs aligned and oriented to the genetic map of the 19 LGs. Distances (shown in brackets on the left for some markers) refer to Troggio et al.'s dense map  (http://genomics.research.iasma.it). Most metacontigs were anchored to the map using markers with unique sequence locations: SSRs, BAC-end sequences or SNP-based markers derived from either ESTs or assembled sequences of the two haplotypes of the Pinot Noir genome. Metacontigs with no marker information were associated to other metacontigs anchored to the map. There are reliable links between them but they are not merged for several reasons, i.e., too large an overlap between them due to some contigs at the end of one metacontig not being in the proper place; gaps too large due to missing contigs; poor quality or insufficient number of links. Approximate size in Kb of each metacontig is indicated on the right. Gaps separating metacontigs are of undefined size.
(2.27 MB TIF)
Grape gene class assignment based on putative function. Functional classification of putative grape genes (total and grape-specific) based on Gene Ontology (www.geneontology.org).
(4.92 MB TIF)
Repetitive element classification and clustering. Phenograms showing the relative similarities of 95 types of repetitive elements out of the 136 identified in the assembled V. vinifera genome. The remaining 41 repeat types (without ORFs or with ORFs shorter than 200 bp) are not included. Repeat types were classified according to Feschotte et al. . Clustering was performed by an all vs all comparison using the BLAST program and was visualized by DrawTree (Myriad Genetics, Salt Lake City, Utah).
(0.65 MB TIF)
Major RNA silencing proteins present in V. vinifera. Major proteins participating in the RNA silencing pathways in V. vinifera have been identified by homology to Arabidopsis proteins using tBLASTN against the V. vinifera genome. Coding sequences of predicted genes were verified in the TC trancripts database. Protein alignments and trees were performed using MEGA version 4 . Aligned protein sequences are: A) Dicer-like proteins (DCLs). Aligned protein sequences are Vitis vinifera putative Dicer-like proteins and A. thaliana DCL1 (At1g01040/Q9SP32), DCL2 (At3g03300/NP_566199), DCL3 (At3g43920/NP_189978) and DCL4 (At5g20320/AAZ80387). B) AGO proteins. Aligned protein sequences are V. vinifera putative Argonaute proteins and A. thaliana AGO1 (At1g48410/NP_849784), AGO2 (At1g31280/NP_174413), AGO3 (At1g31290/NP_174414), AGO4 (At2g27040/NP_565633), AGO5 (At2g27880/Q9SJK3), AGO6 (At2g32940/NP_180853), AGO7 (At1g69440/AAQ92355), AGO8 (At5g21030/NP_197602), AGO9 (At5g21150/CAD66636), and AGO10 (At5g43810/Q9XGW1). C) RNA-dependent-RNA-polymerases (RDRs). Aligned proteins are V. vinifera putative RDRs and A. thaliana RDR1 (AT1g14790/NP_172932), RDR2 (AT4g11130/NP_192851), RDR3 (AT2g19910/NP_179581), RDR4 (AT2g19920/NP_179582), RDR5 (AT2g19930/ NP_179583), and RDR6 (AT3g49500/NP_190519).
(0.11 MB TIF)
Duplicated state of the grape genome. Age distributions of Vitis paralogs (pink line) and Vitis-Arabidopsis orthologs (blue bins).
(0.40 MB TIF)
Details of libraries used in sequencing and estimation of the V. vinifera genome coverage.
(0.03 MB DOC)
Summary of the whole genome shotgun assembly of V. vinifera.
(0.04 MB DOC)
Resistance-related genes of V. vinifera.
(0.10 MB DOC)
Gene family members involved in the core phenylpropanoid pathway, flavonoid and stilbene branches in V. vinifera.
(0.10 MB DOC)
Putative genes encoding enzymes participating in the terpenoid pathway of V. vinifera.
(0.15 MB DOC)
Transcription factors of V. vinifera.
(2.20 MB DOC)
Repetitive elements in the assembled V. vinifera genome.
(0.04 MB DOC)
Microsatellites identified in the assembled V. vinifera genome.
(0.03 MB DOC)
Current state of IASMA database dedicated to V. vinifera mature miRNAs and miRNAs*, including the predicted fold-back structures of the pre-miRNAs.
(0.09 MB DOC)
Number of identified tRNAs containing specified anticodons compared with the corresponding numbers of Arabidopsis.
(0.07 MB DOC)
Number of genes identified in the V. vinifera genome for each of the nine families of snRNAs compared to Arabidopsis.
(0.04 MB DOC)
Special thanks to: David Neale for critical reading of the manuscript, Jessica Zambanini, Monica Dallaserra, Alessandra Zatelli and Michelangelo Policarpo for the technical support.
Conceived and designed the experiments: RV AZ MT DP ST JLS MHS SKB AG FS RV. Performed the experiments: MT DAC MP LMF SV JR GM DI GC BW DM TM MF JTM GE RO NG MS YC CD AM KS QT TH AL CP BT KV RB. Analyzed the data: RV AZ MT DAC AC DP MP SV GM GC DM MF MP PG MM CS JB FC ASA CP BT AS VS JF LS SMG ST CM VS SKB PF AG YVP FS.Contributed reagents/materials/analysis tools: RV AZ AC MS LD AM KS QT TH AL JF LS KV ST RB PF YVP FS RV. Wrote the paper: RV AZ MT DAC AC DP MP SV GM MP PG JB FC ASA BT AS SMG ST CM JLS RB MHS VS SKB PF AG YVP FS RV.
- 1. McGovern PE (2003) Ancient Wine: The Search for the Origins of Viniculture Princeton University Press, Princeton. PE McGovern2003Ancient Wine: The Search for the Origins of Viniculture Princeton University Press, Princeton.
- 2. Panagiotakos DB, Pitsavos C, Polychronopoulos E, Chrysohoou C, Zampelas A, et al. (2004) Can a Mediterranean diet moderate the development and clinical progression of coronary heart disease? A systematic review. Med Sci Monit 10: RA193–198.DB PanagiotakosC. PitsavosE. PolychronopoulosC. ChrysohoouA. Zampelas2004Can a Mediterranean diet moderate the development and clinical progression of coronary heart disease? A systematic review.Med Sci Monit10RA193198
- 3. Burns J, Gardner PT, O'Neil J, Crawford S, Morecroft I, et al. (2000) Relationship among Antioxidant Activity, Vasodilation Capacity, and Phenolic Content of Red Wines. J Agric Food Chem 48: 220–230.J. BurnsPT GardnerJ. O'NeilS. CrawfordI. Morecroft2000Relationship among Antioxidant Activity, Vasodilation Capacity, and Phenolic Content of Red Wines.J Agric Food Chem48220230
- 4. Levadoux L (1956) Les populations sauvages et cultivées de Vitis vinifera L. Ann Amélior Plantes 6: 59–117.L. Levadoux1956Les populations sauvages et cultivées de Vitis vinifera L.Ann Amélior Plantes659117
- 5. Olmo HP (1979) Grapes. In: Simmonds NW, editor. Evolution of crop plants. London.HP Olmo1979Grapes.NW SimmondsEvolution of crop plantsLondon
- 6. Lewis WH (1979) Polyploidy in Angiosperm: dicotyledons. Polyploidy: Biological Relevance. Plenum Press. pp. 241–269.WH Lewis1979Polyploidy in Angiosperm: dicotyledons.Polyploidy: Biological RelevancePlenum Press241269
- 7. Jaillon O, Aury JM, Noel B, Policriti A, Clepet C, et al. (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449: 463–467.O. JaillonJM AuryB. NoelA. PolicritiC. Clepet2007The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla.Nature449463467
- 8. Wang Q, Li P, Hanania U, Sahar N, Mawassi M, et al. (2005) Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Plant Science 168: 565–571.Q. WangP. LiU. HananiaN. SaharM. Mawassi2005Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions.Plant Science168565571
- 9. Kikkert JR, Striem MJ, Vidal JR, Wallace PG, Barnard J, et al. (2005) Long-term study of somatic embryogenesis from anthers and ovaries of 12 grapevine (vitis sp.) Genotypes. In Vitro Cellular and Developmental Biology-Plant 41: 232–239.JR KikkertMJ StriemJR VidalPG WallaceJ. Barnard2005Long-term study of somatic embryogenesis from anthers and ovaries of 12 grapevine (vitis sp.) Genotypes.In Vitro Cellular and Developmental Biology-Plant41232239
- 10. Lodhi MA, Reisch BI (1995) Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae. Theor Appl Genet 90: 11–16.MA LodhiBI Reisch1995Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae.Theor Appl Genet901116
- 11. Goff SA, Ricke D, Lan TH, Presting G, Wang R, et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296: 92–100.SA GoffD. RickeTH LanG. PrestingR. Wang2002A draft sequence of the rice genome (Oryza sativa L. ssp. japonica).Science29692100
- 12. Tuskan GA, DiFazio S, Jansson S, Bohlmann J, Grigoriev I, et al. (2006) The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray). Science 313: 1596–1604.GA TuskanS. DiFazioS. JanssonJ. BohlmannI. Grigoriev2006The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray).Science31315961604
- 13. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A 74: 5463–5467.F. SangerS. NicklenAR Coulson1977DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A7454635467
- 14. Margulies M, Egholm M, Altman WE, Attiya S, Bader JS, et al. (2005) Genome sequencing in microfabricated high-density picolitre reactors. Nature 437: 376–380.M. MarguliesM. EgholmWE AltmanS. AttiyaJS Bader2005Genome sequencing in microfabricated high-density picolitre reactors.Nature437376380
- 15. Troggio M, Malacarne G, Coppola G, Segala C, Cartwright DA, et al. (2007) A Dense Single-Nucleotide Polymorphism-Based Genetic Linkage Map of Grapevine (Vitis vinifera L.) Anchoring Pinot Noir Bacterial Artificial Chromosome Contigs. Genetics 176: 2637–2650.M. TroggioG. MalacarneG. CoppolaC. SegalaDA Cartwright2007A Dense Single-Nucleotide Polymorphism-Based Genetic Linkage Map of Grapevine (Vitis vinifera L.) Anchoring Pinot Noir Bacterial Artificial Chromosome Contigs.Genetics17626372650
- 16. Jones JD, Dangl JL (2006) The plant immune system. Nature 444: 323–329.JD JonesJL Dangl2006The plant immune system.Nature444323329
- 17. Lipka V, Dittgen J, Bednarek P, Bhat R, Wiermer M, et al. (2005) Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis. Science 310: 1180–1183.V. LipkaJ. DittgenP. BednarekR. BhatM. Wiermer2005Pre- and postinvasion defenses both contribute to nonhost resistance in Arabidopsis.Science31011801183
- 18. Stein M, Dittgen J, Sanchez-Rodriguez C, Hou BH, Molina A, et al. (2006) Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration. Plant Cell 18: 731–746.M. SteinJ. DittgenC. Sanchez-RodriguezBH HouA. Molina2006Arabidopsis PEN3/PDR8, an ATP binding cassette transporter, contributes to nonhost resistance to inappropriate pathogens that enter by direct penetration.Plant Cell18731746
- 19. Dangl JL, Jones JD (2001) Plant pathogens and integrated defence responses to infection. Nature 411: 826–833.JL DanglJD Jones2001Plant pathogens and integrated defence responses to infection.Nature411826833
- 20. Takken FL, Albrecht M, Tameling WI (2006) Resistance proteins: molecular switches of plant defence. Curr Opin Plant Biol 9: 383–390.FL TakkenM. AlbrechtWI Tameling2006Resistance proteins: molecular switches of plant defence.Curr Opin Plant Biol9383390
- 21. Meyers BC, Kaushik S, Nandety RS (2005) Evolving disease resistance genes. Curr Opin Plant Biol 8: 129–134.BC MeyersS. KaushikRS Nandety2005Evolving disease resistance genes.Curr Opin Plant Biol8129134
- 22. Bai J, Pennill LA, Ning J, Lee SW, Ramalingam J, et al. (2002) Diversity in nucleotide binding site-leucine-rich repeat genes in cereals. Genome Res 12: 1871–1884.J. BaiLA PennillJ. NingSW LeeJ. Ramalingam2002Diversity in nucleotide binding site-leucine-rich repeat genes in cereals.Genome Res1218711884
- 23. Grant M, Lamb C (2006) Systemic immunity. Curr Opin Plant Biol 9: 414–420.M. GrantC. Lamb2006Systemic immunity.Curr Opin Plant Biol9414420
- 24. van Loon LC, Rep M, Pieterse CM (2006) Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44: 135–162.LC van LoonM. RepCM Pieterse2006Significance of inducible defense-related proteins in infected plants.Annu Rev Phytopathol44135162
- 25. Chen Z, Hartmann HA, Wu MJ, Friedman EJ, Chen JG, et al. (2006) Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Mol Biol 60: 583–597.Z. ChenHA HartmannMJ WuEJ FriedmanJG Chen2006Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins.Plant Mol Biol60583597
- 26. Richter H, Pezet R, Viret O, Gindro K (2006) Characterization of 3 new partial stilbene synthase genes out of over 20 expressed in Vitis vinifera during the interaction with Plasmopara viticola . Physiol Mol Plant Pathol 67: 248–260.H. RichterR. PezetO. ViretK. Gindro2006Characterization of 3 new partial stilbene synthase genes out of over 20 expressed in Vitis vinifera during the interaction with Plasmopara viticola .Physiol Mol Plant Pathol67248260
- 27. Akkurt M, Welter L, Maul E, Topfer R, Zyprian E (2007) Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.). Mol Breed 19: 103–111.M. AkkurtL. WelterE. MaulR. TopferE. Zyprian2007Development of SCAR markers linked to powdery mildew (Uncinula necator) resistance in grapevine (Vitis vinifera L. and Vitis sp.).Mol Breed19103111
- 28. Meyers BC, Shen KA, Rohani P, Gaut BS, Michelmore RW (1998) Receptor-like genes in the major resistance locus of lettuce are subject to divergent selection. Plant Cell 10: 1833–1846.BC MeyersKA ShenP. RohaniBS GautRW Michelmore1998Receptor-like genes in the major resistance locus of lettuce are subject to divergent selection.Plant Cell1018331846
- 29. Mauricio R, Stahl EA, Korves T, Tian D, Kreitman M, et al. (2003) Natural selection for polymorphism in the disease resistance gene Rps2 of Arabidopsis thaliana. Genetics 163: 735–746.R. MauricioEA StahlT. KorvesD. TianM. Kreitman2003Natural selection for polymorphism in the disease resistance gene Rps2 of Arabidopsis thaliana.Genetics163735746
- 30. Stahl EA, Dwyer G, Mauricio R, Kreitman M, Bergelson J (1999) Dynamics of disease resistance polymorphism at the Rpm1 locus of Arabidopsis. Nature 400: 667–671.EA StahlG. DwyerR. MauricioM. KreitmanJ. Bergelson1999Dynamics of disease resistance polymorphism at the Rpm1 locus of Arabidopsis.Nature400667671
- 31. Bakker EG, Toomajian C, Kreitman M, Bergelson J (2006) A genome-wide survey of R gene polymorphisms in Arabidopsis. Plant Cell 18: 1803–1818.EG BakkerC. ToomajianM. KreitmanJ. Bergelson2006A genome-wide survey of R gene polymorphisms in Arabidopsis.Plant Cell1818031818
- 32. Shen J, Araki H, Chen L, Chen JQ, Tian D (2006) Unique evolutionary mechanism in R-genes under the presence/absence polymorphism in Arabidopsis thaliana. Genetics 172: 1243–1250.J. ShenH. ArakiL. ChenJQ ChenD. Tian2006Unique evolutionary mechanism in R-genes under the presence/absence polymorphism in Arabidopsis thaliana.Genetics17212431250
- 33. Clark RM, Schweikert G, Toomajian C, Ossowski S, Zeller G, et al. (2007) Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana. Science 317: 338–342.RM ClarkG. SchweikertC. ToomajianS. OssowskiG. Zeller2007Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana.Science317338342
- 34. Di Gaspero G, Cipriani G, Adam-Blondon A-F, Testolin R (2007) Linkage maps of grapevine displaying the chromosomal locations of 420 microsatellite markers and 82 markers for R -gene candidates. Theor Appl Genet 114: 1249–1263.G. Di GasperoG. CiprianiA-F Adam-BlondonR. Testolin2007Linkage maps of grapevine displaying the chromosomal locations of 420 microsatellite markers and 82 markers for R -gene candidates.Theor Appl Genet11412491263
- 35. Belhadj A, Saigne C, Telef N, Cluzet S, Bouscaut J, et al. (2006) Methyl Jasmonate Induces Defense Responses in Grapevine and Triggers Protection against Erysiphe necator. J Agric Food Chem 54: 9119–9125.A. BelhadjC. SaigneN. TelefS. CluzetJ. Bouscaut2006Methyl Jasmonate Induces Defense Responses in Grapevine and Triggers Protection against Erysiphe necator.J Agric Food Chem5491199125
- 36. Leister D (2004) Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance genes. Trends Genet 20: 116–122.D. Leister2004Tandem and segmental gene duplication and recombination in the evolution of plant disease resistance genes.Trends Genet20116122
- 37. Richly E, Kurth J, Leister D (2002) Mode of amplification and reorganization of resistance genes during recent Arabidopsis thaliana evolution. Mol Biol Evol 19: 76–84.E. RichlyJ. KurthD. Leister2002Mode of amplification and reorganization of resistance genes during recent Arabidopsis thaliana evolution.Mol Biol Evol197684
- 38. Fischer BM, Salakhutdinov I, Akkurt M, Eibach R, Edwards KJ, et al. (2004) Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theor Appl Genet 108: 501–515.BM FischerI. SalakhutdinovM. AkkurtR. EibachKJ Edwards2004Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine.Theor Appl Genet108501515
- 39. Dalbó MA, Ye GN, Weeden NF, Wilcox WF, Reisch BI (2001) Marker-assisted selection for powdery mildew resistance in grapes. J Am Soc Hortic 126: 83–89.MA DalbóGN YeNF WeedenWF WilcoxBI Reisch2001Marker-assisted selection for powdery mildew resistance in grapes.J Am Soc Hortic1268389
- 40. Waterhouse AL (2002) Wine phenolics. Ann N Y Acad Sci 957: 21–36.AL Waterhouse2002Wine phenolics.Ann N Y Acad Sci9572136
- 41. Winkel-Shirley B (2001) Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol 126: 485–493.B. Winkel-Shirley2001Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology.Plant Physiol126485493
- 42. Scalbert A, Manach C, Morand C, Remesy C, Jimenez L (2005) Dietary Polyphenols and the Prevention of Diseases. CRC Crit Rev Food Sci Nutr 45: 287–306.A. ScalbertC. ManachC. MorandC. RemesyL. Jimenez2005Dietary Polyphenols and the Prevention of Diseases.CRC Crit Rev Food Sci Nutr45287306
- 43. Mattivi F, Zulian C, Nicolini G, Valenti L (2002) Wine, biodiversity, technology, and antioxidants. Ann N Y Acad Sci 957: 37–56.F. MattiviC. ZulianG. NicoliniL. Valenti2002Wine, biodiversity, technology, and antioxidants.Ann N Y Acad Sci9573756
- 44. Sparvoli F, Martin C, Scienza A, Gavazzi G, Tonelli C (1994) Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.). Plant Mol Biol 24: 743–755.F. SparvoliC. MartinA. ScienzaG. GavazziC. Tonelli1994Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.).Plant Mol Biol24743755
- 45. Bogs J, Downey MO, Harvey JS, Ashton AR, Tanner GJ, et al. (2005) Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves. Plant Physiol 139: 652–663.J. BogsMO DowneyJS HarveyAR AshtonGJ Tanner2005Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves.Plant Physiol139652663
- 46. Bogs J, Ebadi A, McDavid D, Robinson SP (2006) Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development. Plant Physiol 140: 279–291.J. BogsA. EbadiD. McDavidSP Robinson2006Identification of the flavonoid hydroxylases from grapevine and their regulation during fruit development.Plant Physiol140279291
- 47. Fujita A, Goto-Yamamoto N, Aramaki I, Hashizume K (2006) Organ-specific transcription of putative flavonol synthase genes of grapevine and effects of plant hormones and shading on flavonol biosynthesis in grape berry skins. Biosci Biotechnol Biochem 70: 632–638.A. FujitaN. Goto-YamamotoI. AramakiK. Hashizume2006Organ-specific transcription of putative flavonol synthase genes of grapevine and effects of plant hormones and shading on flavonol biosynthesis in grape berry skins.Biosci Biotechnol Biochem70632638
- 48. Tsai CJ, Harding SA, Tschaplinski TJ, Lindroth RL, Yuan Y (2006) Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus. New Phytol 172: 47–62.CJ TsaiSA HardingTJ TschaplinskiRL LindrothY. Yuan2006Genome-wide analysis of the structural genes regulating defense phenylpropanoid metabolism in Populus.New Phytol1724762
- 49. Schroder G, Brown JW, Schroder J (1988) Molecular analysis of resveratrol synthase. cDNA, genomic clones and relationship with chalcone synthase. Eur J Biochem 172: 161–169.G. SchroderJW BrownJ. Schroder1988Molecular analysis of resveratrol synthase. cDNA, genomic clones and relationship with chalcone synthase.Eur J Biochem172161169
- 50. Jeandet P, Douillet-Breuil A-C, Bessis R, Debord S, Sbaghi M, et al. (2002) Phytoalexins from the Vitaceae: Biosynthesis, Phytoalexin Gene Expression in Transgenic Plants, Antifungal Activity, and Metabolism. J Agric Food Chem 50: 2731–2741.P. JeandetA-C Douillet-BreuilR. BessisS. DebordM. Sbaghi2002Phytoalexins from the Vitaceae: Biosynthesis, Phytoalexin Gene Expression in Transgenic Plants, Antifungal Activity, and Metabolism.J Agric Food Chem5027312741
- 51. Baur JA, Pearson KJ, Price NL, Jamieson HA, Lerin C, et al. (2006) Resveratrol improves health and survival of mice on a high-calorie diet. Nature 444: 337–342.JA BaurKJ PearsonNL PriceHA JamiesonC. Lerin2006Resveratrol improves health and survival of mice on a high-calorie diet.Nature444337342
- 52. Hall D, De Luca V (2007) Mesocarp localization of a bi-functional resveratrol/hydroxycinnamic acid glucosyltransferase of Concord grape (Vitis labrusca). Plant J 49: 579–591.D. HallV. De Luca2007Mesocarp localization of a bi-functional resveratrol/hydroxycinnamic acid glucosyltransferase of Concord grape (Vitis labrusca).Plant J49579591
- 53. Tholl D (2006) Terpene synthases and the regulation, diversity and biological roles of terpene metabolism. Curr Opin Plant Biol 9: 297–304.D. Tholl2006Terpene synthases and the regulation, diversity and biological roles of terpene metabolism.Curr Opin Plant Biol9297304
- 54. Lichtenthaler HK, Rohmer M, Schwender J (1997) Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. Physiol Plant 101: 643–652.HK LichtenthalerM. RohmerJ. Schwender1997Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants.Physiol Plant101643652
- 55. Luan F, Wust M (2002) Differential incorporation of 1-deoxy-D-xylulose into (3S)-linalool and geraniol in grape berry exocarp and mesocarp. Phytochemistry 60: 451–459.F. LuanM. Wust2002Differential incorporation of 1-deoxy-D-xylulose into (3S)-linalool and geraniol in grape berry exocarp and mesocarp.Phytochemistry60451459
- 56. Bohlmann J, Meyer-Gauen G, Croteau R (1998) Plant terpenoid synthases: molecular biology and phylogenetic analysis. Proc Natl Acad Sci U S A 95: 4126–4133.J. BohlmannG. Meyer-GauenR. Croteau1998Plant terpenoid synthases: molecular biology and phylogenetic analysis.Proc Natl Acad Sci U S A9541264133
- 57. McCaskill D, Croteau R (1998) Some caveats for bioengineering terpenoid metabolism in plants. Trends Biotechnol 16: 349–355.D. McCaskillR. Croteau1998Some caveats for bioengineering terpenoid metabolism in plants.Trends Biotechnol16349355
- 58. Martin DM, Bohlmann J (2004) Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase. Phytochemistry 65: 1223–1229.DM MartinJ. Bohlmann2004Identification of Vitis vinifera (-)-alpha-terpineol synthase by in silico screening of full-length cDNA ESTs and functional characterization of recombinant terpene synthase.Phytochemistry6512231229
- 59. Harborne JB (1991) Recent advances in the ecological chemistry of plant terpenoids. Ecological Chemistry and Biochemistry of Plant Terpenoids. Oxford, UK: pp. 399–426.JB Harborne1991Recent advances in the ecological chemistry of plant terpenoids.Ecological Chemistry and Biochemistry of Plant TerpenoidsOxford, UK399426
- 60. Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, et al. (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290: 2105–2110.JL RiechmannJ. HeardG. MartinL. ReuberC. Jiang2000Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.Science29021052110
- 61. Yu J, Wang J, Lin W, Li S, Li H, et al. (2005) The Genomes of Oryza sativa: a history of duplications. PLoS Biol 3: e38.J. YuJ. WangW. LinS. LiH. Li2005The Genomes of Oryza sativa: a history of duplications.PLoS Biol3e38
- 62. Zhu QH, Guo AY, Gao G, Zhong YF, Xu M, et al. (2007) DPTF: a database of poplar transcription factors. Bioinformatics 23: 1307–1308.QH ZhuAY GuoG. GaoYF ZhongM. Xu2007DPTF: a database of poplar transcription factors.Bioinformatics2313071308
- 63. Riano-Pachon DM, Ruzicic S, Dreyer I, Mueller-Roeber B (2007) PlnTFDB: an integrative plant transcription factor database. BMC Bioinformatics 8: 42.DM Riano-PachonS. RuzicicI. DreyerB. Mueller-Roeber2007PlnTFDB: an integrative plant transcription factor database.BMC Bioinformatics842
- 64. Shiu SH, Shih MC, Li WH (2005) Transcription factor families have much higher expansion rates in plants than in animals. Plant Physiol 139: 18–26.SH ShiuMC ShihWH Li2005Transcription factor families have much higher expansion rates in plants than in animals.Plant Physiol1391826
- 65. Deluc L, Barrieu F, Marchive C, Lauvergeat V, Decendit A, et al. (2006) Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol 140: 499–511.L. DelucF. BarrieuC. MarchiveV. LauvergeatA. Decendit2006Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway.Plant Physiol140499511
- 66. Geekiyanage S, Takase T, Ogura Y, Kiyosue T (2007) Anthocyanin production by over-expression of grape transcription factor gene VlmybA2 in transgenic tobacco and Arabidopsis. Plant Biotechnology Reports 1: 11–18.S. GeekiyanageT. TakaseY. OguraT. Kiyosue2007Anthocyanin production by over-expression of grape transcription factor gene VlmybA2 in transgenic tobacco and Arabidopsis.Plant Biotechnology Reports11118
- 67. Bogs J, Jaffe FW, Takos AM, Walker AR, Robinson SP (2007) The grapevine transcription factor VvMYBPA1 regulates proanthocyanidin synthesis during fruit development. Plant Physiol 143: 1347–1361.J. BogsFW JaffeAM TakosAR WalkerSP Robinson2007The grapevine transcription factor VvMYBPA1 regulates proanthocyanidin synthesis during fruit development.Plant Physiol14313471361
- 68. Aharoni A, De Vos CH, Wein M, Sun Z, Greco R, et al. (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28: 319–332.A. AharoniCH De VosM. WeinZ. SunR. Greco2001The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco.Plant J28319332
- 69. Buttner M, Singh KB (1997) Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein. Proc Natl Acad Sci U S A 94: 5961–5966.M. ButtnerKB Singh1997Arabidopsis thaliana ethylene-responsive element binding protein (AtEBP), an ethylene-inducible, GCC box DNA-binding protein interacts with an ocs element binding protein.Proc Natl Acad Sci U S A9459615966
- 70. Rodriguez-Uribe L, O'Connell MA (2006) A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris). J Exp Bot 57: 1391–1398.L. Rodriguez-UribeMA O'Connell2006A root-specific bZIP transcription factor is responsive to water deficit stress in tepary bean (Phaseolus acutifolius) and common bean (P. vulgaris).J Exp Bot5713911398
- 71. Alvarez-Buylla ER, Pelaz S, Liljegren SJ, Gold SE, Burgeff C, et al. (2000) An ancestral MADS-box gene duplication occurred before the divergence of plants and animals. Proc Natl Acad Sci U S A 97: 5328–5333.ER Alvarez-BuyllaS. PelazSJ LiljegrenSE GoldC. Burgeff2000An ancestral MADS-box gene duplication occurred before the divergence of plants and animals.Proc Natl Acad Sci U S A9753285333
- 72. Ng M, Yanofsky MF (2001) Function and evolution of the plant MADS-box gene family. Nat Rev Genet 2: 186–195.M. NgMF Yanofsky2001Function and evolution of the plant MADS-box gene family.Nat Rev Genet2186195
- 73. Vrebalov J, Ruezinsky D, Padmanabhan V, White R, Medrano D, et al. (2002) A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus. Science 296: 343–346.J. VrebalovD. RuezinskyV. PadmanabhanR. WhiteD. Medrano2002A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus.Science296343346
- 74. Giovannoni JJ (2007) Fruit ripening mutants yield insights into ripening control. Curr Opin Plant Biol 10: 283–289.JJ Giovannoni2007Fruit ripening mutants yield insights into ripening control.Curr Opin Plant Biol10283289
- 75. Feschotte C, Jiang N, Wessler SR (2002) Plant transposable elements: where genetics meets genomics. Nat Rev Genet 3: 329–341.C. FeschotteN. JiangSR Wessler2002Plant transposable elements: where genetics meets genomics.Nat Rev Genet3329341
- 76. Jones-Rhoades MW, Bartel DP, Bartel B (2006) MicroRNAS and their regulatory roles in plants. Annu Rev Plant Biol 57: 19–53.MW Jones-RhoadesDP BartelB. Bartel2006MicroRNAS and their regulatory roles in plants.Annu Rev Plant Biol571953
- 77. Sunkar R, Chinnusamy V, Zhu J, Zhu JK (2007) Small RNAs as big players in plant abiotic stress responses and nutrient deprivation. Trends Plant Sci 12: 301–309.R. SunkarV. ChinnusamyJ. ZhuJK Zhu2007Small RNAs as big players in plant abiotic stress responses and nutrient deprivation.Trends Plant Sci12301309
- 78. Griffiths-Jones S (2004) The microRNA Registry. Nucleic Acids Res 32: D109–111.S. Griffiths-Jones2004The microRNA Registry.Nucleic Acids Res32D109111
- 79. Rajagopalan R, Vaucheret H, Trejo J, Bartel DP (2006) A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes Dev 20: 3407–3425.R. RajagopalanH. VaucheretJ. TrejoDP Bartel2006A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana.Genes Dev2034073425
- 80. Fahlgren N, Howell MD, Kasschau KD, Chapman EJ, Sullivan CM, et al. (2007) High-throughput sequencing of Arabidopsis microRNAs: evidence for frequent birth and death of MIRNA genes. PLoS ONE 2: e219.N. FahlgrenMD HowellKD KasschauEJ ChapmanCM Sullivan2007High-throughput sequencing of Arabidopsis microRNAs: evidence for frequent birth and death of MIRNA genes.PLoS ONE2e219
- 81. Axtell MJ, Jan C, Rajagopalan R, Bartel DP (2006) A two-hit trigger for siRNA biogenesis in plants. Cell 127: 565–577.MJ AxtellC. JanR. RajagopalanDP Bartel2006A two-hit trigger for siRNA biogenesis in plants.Cell127565577
- 82. Lowe T, Eddy S (1997) tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res 25: 955–964.T. LoweS. Eddy1997tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence.Nucleic Acids Res25955964
- 83. Wang BB, Brendel V (2004) The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing. Genome Biol 5: R102.BB WangV. Brendel2004The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing.Genome Biol5R102
- 84. Brown JW, Clark GP, Leader DJ, Simpson CG, Lowe T (2001) Multiple snoRNA gene clusters from Arabidopsis. RNA 7: 1817–1832.JW BrownGP ClarkDJ LeaderCG SimpsonT. Lowe2001Multiple snoRNA gene clusters from Arabidopsis.RNA718171832
- 85. Brunner S, Fengler K, Morgante M, Tingey S, Rafalski A (2005) Evolution of DNA sequence nonhomologies among maize inbreds. Plant Cell 17: 343–360.S. BrunnerK. FenglerM. MorganteS. TingeyA. Rafalski2005Evolution of DNA sequence nonhomologies among maize inbreds.Plant Cell17343360
- 86. Rafalski A (2002) Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 5: 94–100.A. Rafalski2002Applications of single nucleotide polymorphisms in crop genetics.Curr Opin Plant Biol594100
- 87. This P, Lacombe T, Thomas MR (2006) Historical origins and genetic diversity of wine grapes. Trends Genet 22: 511–519.P. ThisT. LacombeMR Thomas2006Historical origins and genetic diversity of wine grapes.Trends Genet22511519
- 88. Morgante M, Brunner S, Pea G, Fengler K, Zuccolo A, et al. (2005) Gene duplication and exon shuffling by helitron-like transposons generate intraspecies diversity in maize. Nat Genet 37: 997–1002.M. MorganteS. BrunnerG. PeaK. FenglerA. Zuccolo2005Gene duplication and exon shuffling by helitron-like transposons generate intraspecies diversity in maize.Nat Genet379971002
- 89. Chandler M, Mahillon J (2002) Mobile DNA II. In: Craig NL, Craigie R, Gellert M, Lambowitz AM, editors. Insertion Sequences revisited. American Society for Microbiology. Washington D.C: pp. 305–366.M. ChandlerJ. Mahillon2002Mobile DNA II.NL CraigR. CraigieM. GellertAM LambowitzInsertion Sequences revisited. American Society for MicrobiologyWashington D.C305366
- 90. Sodergren E, Weinstock GM, Davidson EH, Cameron RA, Gibbs RA, et al. (2006) The genome of the sea urchin Strongylocentrotus purpuratus. Science 314: 941–952.E. SodergrenGM WeinstockEH DavidsonRA CameronRA Gibbs2006The genome of the sea urchin Strongylocentrotus purpuratus.Science314941952
- 91. Dehal P, Satou Y, Campbell RK, Chapman J, Degnan B, et al. (2002) The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins. Science 298: 2157–2167.P. DehalY. SatouRK CampbellJ. ChapmanB. Degnan2002The draft genome of Ciona intestinalis: insights into chordate and vertebrate origins.Science29821572167
- 92. Fridman E, Pleban T, Zamir D (2000) A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene. Proc Natl Acad Sci U S A 97: 4718–4723.E. FridmanT. PlebanD. Zamir2000A recombination hotspot delimits a wild-species quantitative trait locus for tomato sugar content to 484 bp within an invertase gene.Proc Natl Acad Sci U S A9747184723
- 93. Thornsberry JM, Goodman MM, Doebley J, Kresovich S, Nielsen D, et al. (2001) Dwarf8 polymorphisms associate with variation in flowering time. Nat Genet 28: 286–289.JM ThornsberryMM GoodmanJ. DoebleyS. KresovichD. Nielsen2001Dwarf8 polymorphisms associate with variation in flowering time.Nat Genet28286289
- 94. Lindblad-Toh K, Wade CM, Mikkelsen TS, Karlsson EK, Jaffe DB, et al. (2005) Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature 438: 803–819.K. Lindblad-TohCM WadeTS MikkelsenEK KarlssonDB Jaffe2005Genome sequence, comparative analysis and haplotype structure of the domestic dog.Nature438803819
- 95. Venter JC, Adams MD, Myers EW, Li PW, Mural RJ, et al. (2001) The sequence of the human genome. Science 291: 1304–1351.JC VenterMD AdamsEW MyersPW LiRJ Mural2001The sequence of the human genome.Science29113041351
- 96. Holt RA, Subramanian GM, Halpern A, Sutton GG, Charlab R, et al. (2002) The genome sequence of the malaria mosquito Anopheles gambiae. Science 298: 129–149.RA HoltGM SubramanianA. HalpernGG SuttonR. Charlab2002The genome sequence of the malaria mosquito Anopheles gambiae.Science298129149
- 97. Simillion C, Vandepoele K, Van Montagu MC, Zabeau M, Van de Peer Y (2002) The hidden duplication past of Arabidopsis thaliana. Proc Natl Acad Sci U S A 99: 13627–13632.C. SimillionK. VandepoeleMC Van MontaguM. ZabeauY. Van de Peer2002The hidden duplication past of Arabidopsis thaliana.Proc Natl Acad Sci U S A991362713632
- 98. Bowers JE, Chapman BA, Rong J, Paterson AH (2003) Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature 422: 433–438.JE BowersBA ChapmanJ. RongAH Paterson2003Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events.Nature422433438
- 99. De Bodt S, Maere S, Van de Peer Y (2005) Genome duplication and the origin of angiosperms. Trends Ecol Evol 20: 591–597.S. De BodtS. MaereY. Van de Peer2005Genome duplication and the origin of angiosperms.Trends Ecol Evol20591597
- 100. Jansen RK, Kaittanis C, Saski C, Lee SB, Tomkins J, et al. (2006) Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids. BMC Evol Biol 6: 32.RK JansenC. KaittanisC. SaskiSB LeeJ. Tomkins2006Phylogenetic analyses of Vitis (Vitaceae) based on complete chloroplast genome sequences: effects of taxon sampling and phylogenetic methods on resolving relationships among rosids.BMC Evol Biol632
- 101. Korf I, Flicek P, Duan D, Brent MR (2001) Integrating genomic homology into gene structure prediction. Bioinformatics 17: S140–148.I. KorfP. FlicekD. DuanMR Brent2001Integrating genomic homology into gene structure prediction.Bioinformatics17S140148
- 102. Simillion C, Vandepoele K, Saeys Y, Van de Peer Y (2004) Building genomic profiles for uncovering segmental homology in the twilight zone. Genome Res 14: 1095–1106.C. SimillionK. VandepoeleY. SaeysY. Van de Peer2004Building genomic profiles for uncovering segmental homology in the twilight zone.Genome Res1410951106
- 103. Van de Peer Y, Taylor JS, Braasch I, Meyer A (2001) The ghost of selection past: rates of evolution and functional divergence of anciently duplicated genes. J Mol Evol 53: 436–446.Y. Van de PeerJS TaylorI. BraaschA. Meyer2001The ghost of selection past: rates of evolution and functional divergence of anciently duplicated genes.J Mol Evol53436446
- 104. Byrne KP, Wolfe KH (2007) Consistent patterns of rate asymmetry and gene loss indicate widespread neofunctionalization of yeast genes after whole-genome duplication. Genetics 175: 1341–1350.KP ByrneKH Wolfe2007Consistent patterns of rate asymmetry and gene loss indicate widespread neofunctionalization of yeast genes after whole-genome duplication.Genetics17513411350
- 105. Maere S, De Bodt S, Raes J, Casneuf T, Van Montagu M, et al. (2005) Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci U S A 102: 5454–5459.S. MaereS. De BodtJ. RaesT. CasneufM. Van Montagu2005Modeling gene and genome duplications in eukaryotes.Proc Natl Acad Sci U S A10254545459
- 106. Grando MS, Bellin D, Edwards KJ, Pozzi C, Stefanini M, et al. (2003) Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx. Theor Appl Genet 106: 1213–1224.MS GrandoD. BellinKJ EdwardsC. PozziM. Stefanini2003Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx.Theor Appl Genet10612131224
- 107. Doucleff M, Jin Y, Gao F, Riaz S, Krivanek AF, et al. (2004) A genetic linkage map of grape, utilizing Vitis rupestris and Vitis arizonica. Theor Appl Genet 109: 1178–1187.M. DoucleffY. JinF. GaoS. RiazAF Krivanek2004A genetic linkage map of grape, utilizing Vitis rupestris and Vitis arizonica.Theor Appl Genet10911781187
- 108. Lowe KM, Walker MA (2006) Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) x Riparia Gloire (Vitis riparia). Theor Appl Genet 112: 1582–1592.KM LoweMA Walker2006Genetic linkage map of the interspecific grape rootstock cross Ramsey (Vitis champinii) x Riparia Gloire (Vitis riparia).Theor Appl Genet11215821592
- 109. Adam-Blondon AF, Bernole A, Faes G, Lamoureux D, Pateyron S, et al. (2005) Construction and characterization of BAC libraries from major grapevine cultivars. Theor Appl Genet 110: 1363–1371.AF Adam-BlondonA. BernoleG. FaesD. LamoureuxS. Pateyron2005Construction and characterization of BAC libraries from major grapevine cultivars.Theor Appl Genet11013631371
- 110. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.JJ DoyleJL Doyle1987A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochemical Bulletin191115
- 111. Tobler AR, Short S, Andersen MR, Paner TM, Briggs JC, et al. (2005) The SNPlex genotyping system: a flexible and scalable platform for SNP genotyping. J Biomol Tech 16: 398–406.AR ToblerS. ShortMR AndersenTM PanerJC Briggs2005The SNPlex genotyping system: a flexible and scalable platform for SNP genotyping.J Biomol Tech16398406
- 112. Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137: 1121–1137.D. GrattapagliaR. Sederoff1994Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers.Genetics13711211137
- 113. Cartwright DA, Troggio M, Velasco R, Gutin A (2007) Genetic Mapping in the Presence of Genotyping Errors. Genetics 176: 2521–2527.DA CartwrightM. TroggioR. VelascoA. Gutin2007Genetic Mapping in the Presence of Genotyping Errors.Genetics17625212527
- 114. Solovyev V, Kosarev P, Seledsov I, Vorobyev D (2006) Automatic annotation of eukaryotic genes, pseudogenes and promoters. Genome Biol 7 Suppl 1: S10.1–12.V. SolovyevP. KosarevI. SeledsovD. Vorobyev2006Automatic annotation of eukaryotic genes, pseudogenes and promoters.Genome Biol 7 Suppl1S10.112
- 115. Majoros WH, Pertea M, Salzberg SL (2004) TigrScan and GlimmerHMM: two open source ab initio eukaryotic gene-finders. Bioinformatics 20: 2878–2879.WH MajorosM. PerteaSL Salzberg2004TigrScan and GlimmerHMM: two open source ab initio eukaryotic gene-finders.Bioinformatics2028782879
- 116. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.SF AltschulTL MaddenAA SchafferJ. ZhangZ. Zhang1997Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic Acids Res2533893402
- 117. Huang X, Madan A (1999) CAP3: A DNA Sequence Assembly Program. Genome Res 9: 868–877.X. HuangA. Madan1999CAP3: A DNA Sequence Assembly Program.Genome Res9868877
- 118. Mulder NJ, Apweiler R, Attwood TK, Bairoch A, Bateman A, et al. (2007) New developments in the InterPro database. Nucleic Acids Res 35: D224–228.NJ MulderR. ApweilerTK AttwoodA. BairochA. Bateman2007New developments in the InterPro database.Nucleic Acids Res35D224228
- 119. Lord PW, Stevens RD, Brass A, Goble CA (2003) Investigating semantic similarity measures across the Gene Ontology: the relationship between sequence and annotation. Bioinformatics 19: 1275–1283.PW LordRD StevensA. BrassCA Goble2003Investigating semantic similarity measures across the Gene Ontology: the relationship between sequence and annotation.Bioinformatics1912751283
- 120. Li WH, Gu Z, Wang H, Nekrutenko A (2001) Evolutionary analyses of the human genome. Nature 409: 847–849.WH LiZ. GuH. WangA. Nekrutenko2001Evolutionary analyses of the human genome.Nature409847849
- 121. Felsenstein J (1989) PHYLIP—Phylogeny Inference Package. Cladistics 5: 164–166.J. Felsenstein1989PHYLIP—Phylogeny Inference Package.Cladistics5164166
- 122. Finn RD, Mistry J, Schuster-Bockler B, Griffiths-Jones S, Hollich V, et al. (2006) Pfam: clans, web tools and services. Nucleic Acids Res 34: D247–251.RD FinnJ. MistryB. Schuster-BocklerS. Griffiths-JonesV. Hollich2006Pfam: clans, web tools and services.Nucleic Acids Res34D247251
- 123. Henikoff S, Henikoff JG (1994) Position-based sequence weights. J Mol Biol 243: 574–578.S. HenikoffJG Henikoff1994Position-based sequence weights.J Mol Biol243574578
- 124. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25: 4876–4882.JD ThompsonTJ GibsonF. PlewniakF. JeanmouginDG Higgins1997The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.Nucleic Acids Res2548764882
- 125. Notredame C , Higgins DG, Heringa J (2000) T-Coffee: A novel method for fast and accurate multiple sequence alignment. J Mol Biol 302: 205–217.Notredame CDG HigginsJ. Heringa2000T-Coffee: A novel method for fast and accurate multiple sequence alignment.J Mol Biol302205217
- 126. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Mol Biol Evol. K. TamuraJ. DudleyM. NeiS. Kumar2007MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0.Mol Biol Evol
- 127. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.JD ThompsonDG HigginsTJ Gibson1994CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.Nucleic Acids Res2246734680
- 128. Adam-Blondon AF, Roux C, Claux D, Butterlin G, Merdinoglu D, et al. (2004) Mapping 245 SSR markers on the Vitis vinifera genome: a tool for grape genetics. Theor Appl Genet 109: 1017–1027.AF Adam-BlondonC. RouxD. ClauxG. ButterlinD. Merdinoglu2004Mapping 245 SSR markers on the Vitis vinifera genome: a tool for grape genetics.Theor Appl Genet10910171027
- 129. Jones-Rhoades MW, Bartel DP (2004) Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol Cell 14: 787–799.MW Jones-RhoadesDP Bartel2004Computational identification of plant microRNAs and their targets, including a stress-induced miRNA.Mol Cell14787799