Microsporidia are intracellular parasites that are highly-derived relatives of fungi. They have compacted genomes and, despite a high rate of sequence evolution, distantly related species can share high levels of gene order conservation. To date, only two species have been analysed in detail, and data from one of these largely consists of short genomic fragments. It is therefore difficult to determine how conservation has been maintained through microsporidian evolution, and impossible to identify whether certain regions are more prone to genomic stasis.
Here, we analyse three large fragments of the Enterocytozoon bieneusi genome (in total 429 kbp), a species of medical significance. A total of 296 ORFs were identified, annotated and their context compared with Encephalitozoon cuniculi and Antonospora locustae. Overall, a high degree of conservation was found between all three species, and interestingly the level of conservation was similar in all three pairwise comparisons, despite the fact that A. locustae is more distantly related to E. cuniculi and E. bieneusi than either are to each other.
Any two genes that are found together in any pair of genomes are more likely to be conserved in the third genome as well, suggesting that a core of genes tends to be conserved across the entire group. The mechanisms of rearrangments identified among microsporidian genomes were consistent with a very slow evolution of their architecture, as opposed to the very rapid sequence evolution reported for these parasites.
Citation: Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. (2007) Patterns of Genome Evolution among the Microsporidian Parasites Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi. PLoS ONE2(12): e1277. https://doi.org/10.1371/journal.pone.0001277
Academic Editor: Geraldine Butler, University College Dublin, Ireland
Received: October 1, 2007; Accepted: November 9, 2007; Published: December 5, 2007
Copyright: © 2007 Corradi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The Enterocytozoon bieneusi genome sequence survey was supported by National Institutes of Health grants R21 AI52792 and R21 AI064118 and analysis was supported by a grant from the Canadian Institutes for Heath Research (MOP-84265). We acknowledge the Josephine Bay Paul Center for Comparative Molecular Biology and Evolution for the use of data included in the Antonospora locustae Genome Project funded by NSF award number 0135272. NC is supported by a fellowship from the Swiss National Science Foundation (PBLAA-114238). PJK is a fellow of the Canadian Institute for Advanced Research and the Michael Smith Foundation for Health Research. None of these institutions played a role in the design and conduct of the study, in the collection, analysis and interpretation of the data and in the preparation, review or approval of this manuscript.
Competing interests: The authors have declared that no competing interests exist.
Microsporidia are a diverse group of intracellular eukaryotic parasites including 1,300 described species , over a dozen of which are known to infect humans . These organisms were once regarded as primitively simple eukaryotes, however it is now widely acknowledged that they are extremely specialized, highly derived relatives of fungi –. Microsporidia are known to harbour very small genomes, the most extreme being the 2.3 Mbp genome of Encephalitozoon intestinalis . Microsporidian genomes have shrunk in two different ways. First, their obligate intracellular parasitic lifestyle has permitted the loss of many genes whose functions can be provided by the host cell, so that that the number of genes is severely reduced, in the case of the complete genome of E. cuniculi to about 2,000 genes , . Second, those genes that remain have been packed into a smaller space by a reduction in the overall size of genes (due to a paucity of introns and slightly smaller proteins) and by a significant reduction in intergenic spaces , .
A survey of genomic sequences from Antonospora locustae, a microsporidian that is distantly related to E. cuniculi, suggested that this compaction has further effects on the evolution of microsporidian genomes. Specifically, this survey showed that the order of genes in the A. locustae and E. cuniculi genomes share a high degree of conservation, and that this conservation was correlated with short intergenic spaces . Conservation of gene order usually degrades rapidly in eukaryotic genomes , , leading to the suggestion that genome compaction could slow the rate of genome rearrangements . This comparison also challenged the hypothesis that the rate of genome evolution may be correlated with the rate of sequence evolution , since the rate of sequence evolution between the two species is relatively high .
The previous study raised a number of questions about genome evolution in microsporidia and other compacted nuclear genomes that cannot be addressed using a two-way comparison. For example, if gene order conservation is conserved in other microsporidia, are the same gene clusters preserved among many genomes, or merely some level of conservation across the genome? When genes do move, do they move individually or in blocks? Similarly, does the conservation between two genomes correlate with their evolutionary relatedness, or is conservation of gene order driven by other evolutionary mechanisms such as gene co-expression  and recombination rate ? Surveying a third microsporidian genome has the potential to address some of these questions and reveal important aspects of other processes that may be related to genome compaction in microsporidia , , , as well as revealing whether conserved gene clusters are special, or only part of a more random but global process.
Enterocytozoon bieneusi is a frequent and problematic pathogen of HIV-infected patients ,  and its phylogenetic position is slightly closer to E. cuniculi than to A. locustae , making this an ideal species from which to compare the conservation of gene order within the microsporidia. In this study, which is part of an ongoing genome sequence survey of E. bieneusi, three large supercontigs encompassing 429 kbp were annotated and compared to both E. cuniculi and A. locustae. E. bieneusi genes were mapped to homologues in the E. cuniculi genome to determine the overall level of conservation between these two species. These gene order maps were also used to identify conserved blocks of genes and how genes that are not conserved cluster elsewhere in the genome. Finally, a three-way comparison including A. locustae was used to assess how genome rearrangements evolved along the phylogenetic tree of microsporidia.
Results and Discussions
Evolution of genome structure between E. bieneusi and E. cuniculi
Open reading frames were identified on three contiguous fragments of the E. bieneusi genome, which encode 116, 127, and 186 Kbp. A total of 296 open reading frames were identified and, for each of these putative genes, the position of orthologous genes in the E. cuniculi genome was determined. In the E. bieneusi genome, 83% of all the genes found in these blocks have a clearly recognisable orthologue in E. cuniculi and these orthologues are scattered across the genome. The remaining 17% of the ORFs identified showed little similarity to E. cuniculi ORFs, a consequence of either high sequence divergence among microsporidian genes  or independent insertions/deletions of genes along their evolutionary history. A careful analysis of these latter ORFs showed that some were indeed true orthologues, that they shared synteny with other genes in E. cuniculi and that the low similarity identified between the orthologues was a consequence of very high sequence divergence.
Overall, the E. bieneusi genome is compact, with around 0.7 genes per kilobase, but not as compact as E. cuniculi (∼0.97 genes per kilobase, . Genetic distance between these two species is comparable with what has been observed between A. locustae and E. cuniculi  (p-distance of 0.52 averaged over 14 protein-coding genes). We found no evidence for repetitive and mobile DNA in the genomic regions we analysed, although some examples are known in A. locustae , Vairimorpha corneae , Spraguea lophii  and Nosema bombyicis . Based on the identified orthologues, we first measured the degree of conservation of gene order as the percentage of adjacent gene pairs in E. bieneusi that were also adjacent or close neighbors in E. cuniculi. From 293 comparable E. bieneusi adjacent gene pairs, 16% were also adjacent in E. cuniculi, an additional 14% were close neighbors in E. cuniculi. Overall, 40% of the E. bieneusi paired genes were located on the same chromosome in E. cuniculi (Fig. 1). This shows that the degree of conservation is comparable to that previously reported for the E. cuniculi–A. locustae comparison  change to numerical citation format and that a relatively high level of genome order conservation therefore extends to E. bieneusi.
Each category displays the percentage of gene pairs in E. bieneusi that are adjacent, close neighbours, or nonsyntenic in E. cuniculi.
The comparison between E. cuniculi and A. locustae was primarily based on small regions of the A. locustae genome. The large E. bieneusi fragments allow insights not possible with these small fragments, so we compared in detail the order and orientation of the 296 E. bieneusi ORFs with homologous regions of the E. cuniculi genome (Figs. 2–4) to see what kinds of rearrangements are most common. As previously determined for other species , but at a much larger evolutionary scale, these comparisons reveal very little conservation at the highest level (i.e., for each E. bieneusi fragment the genes are found on many E. cuniculi chromosomes), but there were many blocks of genes where local gene order is highly conserved. Numerous small rearrangement events have also occurred within regions of conserved gene order, many including small inversions of one or more genes between E. bieneusi and E. cuniculi. This has also been observed in closely related eukaryotic genomes and is thought to be a widespread evolutionary mechanism in eukaryotic nuclear genomes , –. Larger inversions are also seen, including the complete inversions of syntenic regions of as many as seven ORFs (e.g., Fig. 3). Single-ORF transpositions are also evident in some cases (e.g., Ecu03_240 in Figure 2 and Ecu07_0270 in Figure 4).
Coloured boxes represent orthologues in the same context in both genomes, and the chromosome on which each E. cuniculi segment is located is indicated by Roman numerals. Grey boxes represent genes that are not identifiably orthologous or are not conserved in context. Regions of both genomes transposed above or below the aligned regions represent blocks of genes in different contexts. The chromosomal location of the E. cuniculi orthologue of such genes from E. bieneusi is indicated by Arabic numerals above the boxes. The chromosomal location of syntenic ORFs in the genome of E. cuniculi is shown in the lower part of the figure and annotated with Roman numbers. Straight lines join the homologous ORFs. Red triangles represent boundaries between the contigs used to generate the scaffolds presented in these figures.
The blocks of conservation are separated by regions of variable sizes where genes show no conservation of order. Many such regions are composed of a single gene or small number of genes, but in several, blocks of multiple genes exist where no conservation of order is found (i.e., grey genes above the aligned regions of the genomes in Figs. 2–4). The ORFs in which order is reciprocally conserved in E. cuniculi, but not in E. bieneusi, cannot be determined until the complete genome is annotated. However, both genomes appear to have been rearranged in blocks of genes where there are a few instances of reorganisation, and blocks of genes where there are few instances of conservation.
Genome conservation across the tree of microsporidia
A schematic representation of microsporidia phylogeny based on SSU phylogenies is shown in Figure 5 , . Molecular phylogeny consistently places the Encephalitozoon clade as sister to the Nosema clade, and the Enterocytozoon clade as sister to both. Antonospora falls elsewhere in the tree as sister to Paranosema, and is therefore more distantly related to either E. cuniculi or E. bieneusi than they are to one another. Generally, conservation of gene order correlates with the evolutionary distance between the species , , so one might predict a significantly higher level of conservation between E. cuniculi and E. bieneusi than between either of these and A. locustae. Indeed, E. bieneusi and E. cuniculi do share a slightly higher proportion of adjacent gene pairs (16%, Fig. 1) compared to that found between E. cuniculi and A. locustae (13%, ), but the difference is small and the level of conservation of more distant pairs is actually lower (40% and 44%, respectively, Fig. 1). Overall, there is no strong evidence for a correlation between the level of gene order conservation and the evolutionary distances between the organisms, suggesting as an alternative that the level of conservation might be static.
Colored boxes linking pairs of taxa indicate the percentage of conservation of adjacent pairs of genes. Blue boxes indicate the percentage of adjacent loci that are also adjacent in the third microsporidian species analysed in this study.
Evidence for a core of gene pairs with conserved order among microsporidia
To more fully analyse the nature of genome conservation between all three species, we conducted a parallel analysis of genes in complete adjacency between E. bieneusi and A. locustae using a dataset including 94 gene pairs previously analysed in A. locustae . The conservation of adjacent pairs was calculated for each of three possible pairwise species comparisons, resulting in values similar to those reported above and previously (Fig. 5). Specifically, the percent conservation of E. cuniculi and E. bieneusi to A. locustae were 13% and 12%, respectively, while the conservation between E. cuniculi and E. bieneusi to one another was 16%. The important question arising from this is whether the same genes tend to be conserved in order in all three genomes. To test this, we took all conserved adjacent gene pairs from each of the three pairwise species comparisons, and determined the frequency with which they were also conserved in the third species (e.g. for the 16% of gene pairs conserved between E. bieneusi and E. cuniculi, homologues for all genes were identified in A. locustae and the frequency with which the same pairs were conserved was determined). Interestingly, this frequency was significantly higher than the baseline frequency for all three of the pairwise species comparisons (i.e., 42% to 52%: Fig. 5). This result suggests that there is a core of gene pairs that were most likely linked in the ancestor of all three species, and are much more likely to remain linked than other genes in the genome (for examples, see Supporting Information File S1). This is also consistent with the lack of any pronounced correlation between the level of genome conservation and the evolutionary distance beween any two species. This correlation is likely more obvious in closely related species, but between such distant relatives, it could be overwhelmed by a relatively larger pool of genes that tend to be conserved throughout the group. Interestingly, when the regions conserved in all three species are compared, it is difficult to determine which species are most similar in genomic structure. In some cases, genomic architecture seems to be more similar between E. cuniculi and E. bieneusi (Supporting Information File S1, regions B, E, F), while in others, E. cuniculi and A. locustae share a more similar genomic context (Supporting Information File S1, regions C, I, J).
Possible causes and mechanisms leading to genomic stasis in microsporidia
Examples of gene order conservation at deep levels of divergence are known in a few cases where order is maintained either by chance , because the proteins are part of a complex regulatory pathway  or because of coregulation of functionally associated genes , . However, with the exception of two loci encoding ribosomal proteins, neither of the latter predictions seem to account for the conserved gene order across the three microsporidian species. Using bioinformatics tools such as InterproScan  we failed to identify any conserved gene pairs that appear to be part of a same gene network, or which are known to be functionally associated. Conservation was shown to be correlated with short intergenic regions in E. cuniculi and A. locustae and it was proposed that the compaction of the genome made it difficult to separate genes without introducing deleterious breakpoints , a factor noted to contribute to conservation of gene order in other genomes as well . The recently discovered phenomenon of multigene transcription in microsporidia ,  could also contribute to the preservation of gene order, since control elements of one gene may be situated within an adjacent gene, selecting against their separation. Indeed, three gene pairs that we have identified as being conserved across all three species (Supporting Information File S1, regions I, J, K), have also been found to show overlapping transcription in A. locustae  and in E. cuniculi (unpublished). This correlation would extend the idea that co-expression of genes in yeast leads to greater conservation in order , however, large scale analyses of transcriptional patterns among syntenic genes in the microsporidia are necessary to determine if a correlation between multigene transcription and conservation of gene order is a significant factor or a minor one.
One correlation that clearly does not hold is the proposed relationship between the rates of nucleotide substitution and genomic rearrangements, which are thought to correlate in other organisms , , . From that point of view, microsporidia can be considered as exceptional for harbouring very fast evolving genes  within slowly evolving genomes. According to these results, it is tempting to speculate that two independent evolutionary forces are acting on microsporidia genes and their genome structure.
Materials and Methods
E. bieneusi genomic DNA isolation, genomic library construction and sequencing
The genomic data used in the study come from a genome survey project funded by NIH grants to the Tufts University group (manuscript in preparation). E. bieneusi spores were purified from fresh stools of infected adult humans using the method described by Zhang and colleagues . Two independent genomic DNA extractions were carried out with using a modified proteinase K-phenol extraction protocol . Using the two genomic DNA preparations, two random 2–3 kb genomic libraries were constructed by Agencourt Biosciences, Inc. (Beverly, MA) in a proprietary high copy number vector. In the construction of the library, genomic DNA was hydrodynamically sheared in the Hydroshear (Genomic Solutions, Ann Arbor, MI) and then separated on agarose gel. A fraction corresponding to ∼3,500 bp in length was excised from the gel and purified by the GeneClean procedure (Qbiogene, Morgan Irvine, CA). The purified DNA fragments were blunt-ended using T4 DNA polymerase. The 3.5 kb DNA was ligated to unique BstXI-linker adapters and the linker-adapted inserts were ligated to BstXI-cut vector to construct a “shotgun” library. Clones were sequenced using ABI 3.1 BigDye terminator chemistry (Applied Biosystems, Foster City, CA). In total 37,383 reads were assembled into 2,821 contiguously assembled segments. consisting of two or more reads, using the Paracel GenomeAssembler (PGA) (http://www.paracel.com/products/pga.html) with default program parameters and quality scores. 1,078 contigs were discarded as contaminating bacterial genomic sequence, leaving 1,743 E. bieneusi contigs. This Whole Genome Shotgun project has been deposited at DDBJ/EMBL/GenBank under the project accession ABGB00000000. The version described in this paper is the first version, ABGB01000000. Three large scaffolds (SC_2384, SC_2496 and SC_1888) were obtained based on contig mate pair information and used in this study. SC_2384 was assembled from contigs ctg01_153 (43326 bp) and ctg01_154 (83950 bp). SC_2496 was assembled from contigs ctg01_1952 (65945 bp), ctg01_2519 (1260 bp), ctg01_2570 (1227 bp), ctg01_2003 (1338 bp) and ctg01_1951 (118303 bp). SC_1888 was assembled from contigs ctg01_110 (2152 bp), 111 (3690 bp), 112 (6021 bp), 113 (3057 bp), 114 (2484 bp) and 115 (98412 bp). All the contigs used in this study are included in accession ABGB00000000.
Annotation of E. bieneusi supercontigs and gene order surveys
Putative open reading frames (ORFs) were characterized in this study from the three E. bieneusi scaffolds (Supporting Information File S2). The genomic DNA sequence of the contigs was compared to the complete genome of E. cuniculi  using tBLASTX ,  to identify all potential protein-coding genes. The list of putative ORFs identified along the three contigs, their description, their position and the accession number of their relative ORF in the E. cuniculi genome are listed in the supporting information file, Table S1. In parallel, to compare the level of conservation between E. bieneusi and A. locustae, the gene pairs used in the study by Slamovits et al. (2004) change to numerical citation were compared to the E. bieneusi genome database using tBLASTX. As this comparison was performed before the E. bieneusi genome was completely assembled, we limited our analysis to whether gene pairs were adjacent (no intervening ORFs) in each species, to avoid any biases due to contigs with different lengths or with incomplete ORF annotations. Finally, the set of adjacent gene pairs found in common between E. bieneusi and E. cuniculi were compared to the A. locustae genome database (http://gmod.mbl.edu/perl/site/antonospora), to determine the number of gene pairs conserved in order among all the three microsporidian species we analysed in this study (Fig. 5, percentages in blue squares). Function predictions were generated for the sets of syntenic proteins using Interproscan .
Examples of gene order conservation between E. cuniculi, E. bieneusi and A. locustae. This figure represent genomic regions of A. locustae previously identified by Slamovits et al. (2004) and Williams et al. (2004) numerical citations. Loci in the same order are shown in coloured arrows and are linked with straight lines. Transcriptional direction of genes is indicated by arrow direction. The accession numbers of A. locustae fragments shown in this figure are as follows. A. AY548887, B. AY548895, C. AY548905, D. AY548901, F. AY548898, G. AY548891, H. AY548889, I. DQ057555, J. DQ057548, K. DQ057569
(0.09 MB DOC)
Genomic sequence of the of the scaffolds used in this study.
(0.46 MB DOC)
We thank Claudio Slamovits, James Harper and three reviewers for critical readings of this manuscript.
Conceived and designed the experiments: PK NC. Analyzed the data: NC. Contributed reagents/materials/analysis tools: LW ST HM DA XF. Wrote the paper: PK NC.
- 1. Larsson JIR (1999) Identification of microsporidia. Acta Protozoologica 38: 161–197.JIR Larsson1999Identification of microsporidia.Acta Protozoologica38161197
- 2. Franzen C, Muller A (2001) Microsporidiosis: human diseases and diagnosis. Microbes Infect 3: 389–400.C. FranzenA. Muller2001Microsporidiosis: human diseases and diagnosis.Microbes Infect3389400
- 3. Keeling PJ (2003) Congruent evidence from alpha-tubulin and beta-tubulin gene phylogenies for a zygomycete origin of microsporidia. Fungal Genet Biol 38: 298–309.PJ Keeling2003Congruent evidence from alpha-tubulin and beta-tubulin gene phylogenies for a zygomycete origin of microsporidia.Fungal Genet Biol38298309
- 4. Keeling PJ, Doolittle WF (1996) Alpha-tubulin from early-diverging eukaryotic lineages and the evolution of the tubulin family. Mol Biol Evol 13: 1297–1305.PJ KeelingWF Doolittle1996Alpha-tubulin from early-diverging eukaryotic lineages and the evolution of the tubulin family.Mol Biol Evol1312971305
- 5. Keeling PJ, Luker MA, Palmer JD (2000) Evidence from beta-tubulin phylogeny that microsporidia evolved from within the fungi. Mol Biol Evol 17: 23–31.PJ KeelingMA LukerJD Palmer2000Evidence from beta-tubulin phylogeny that microsporidia evolved from within the fungi.Mol Biol Evol172331
- 6. Van de Peer Y, Ben Ali A, Meyer A (2000) Microsporidia: accumulating molecular evidence that a group of amitochondriate and suspectedly primitive eukaryotes are just curious fungi. Gene 246: 1–8.Y. Van de PeerA. Ben AliA. Meyer2000Microsporidia: accumulating molecular evidence that a group of amitochondriate and suspectedly primitive eukaryotes are just curious fungi.Gene24618
- 7. Peyretaillade E, Biderre C, Peyret P, Duffieux F, Metenier G, et al. (1998) Microsporidian Encephalitozoon cuniculi, a unicellular eukaryote with an unusual chromosomal dispersion of ribosomal genes and a LSU rRNA reduced to the universal core. Nucleic Acids Res 26: 3513–3520.E. PeyretailladeC. BiderreP. PeyretF. DuffieuxG. Metenier1998Microsporidian Encephalitozoon cuniculi, a unicellular eukaryote with an unusual chromosomal dispersion of ribosomal genes and a LSU rRNA reduced to the universal core.Nucleic Acids Res2635133520
- 8. Katinka MD, Duprat S, Cornillot E, Metenier G, Thomarat F, et al. (2001) Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi. Nature 414: 450–453.MD KatinkaS. DupratE. CornillotG. MetenierF. Thomarat2001Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi.Nature414450453
- 9. Vivares CP, Metenier G (2000) Towards the minimal eukaryotic parasitic genome. Curr Opin Microbiol 3: 463–467.CP VivaresG. Metenier2000Towards the minimal eukaryotic parasitic genome.Curr Opin Microbiol3463467
- 10. Slamovits CH, Fast NM, Law JS, Keeling PJ (2004) Genome compaction and stability in microsporidian intracellular parasites. Curr Biol 14: 891–896.CH SlamovitsNM FastJS LawPJ Keeling2004Genome compaction and stability in microsporidian intracellular parasites.Curr Biol14891896
- 11. Seoighe C, Federspiel N, Jones T, Hansen N, Bivolarovic V, et al. (2000) Prevalence of small inversions in yeast gene order evolution. Proc Natl Acad Sci U S A 97: 14433–14437.C. SeoigheN. FederspielT. JonesN. HansenV. Bivolarovic2000Prevalence of small inversions in yeast gene order evolution.Proc Natl Acad Sci U S A971443314437
- 12. Huynen MA, Snel B, Bork P (2001) Inversions and the dynamics of eukaryotic gene order. Trends Genet 17: 304–306.MA HuynenB. SnelP. Bork2001Inversions and the dynamics of eukaryotic gene order.Trends Genet17304306
- 13. Suyama M, Bork P (2001) Evolution of prokaryotic gene order: genome rearrangements in closely related species. Trends Genet 17: 10–13.M. SuyamaP. Bork2001Evolution of prokaryotic gene order: genome rearrangements in closely related species.Trends Genet171013
- 14. Hurst LD, Williams EJ, Pal C (2002) Natural selection promotes the conservation of linkage of co-expressed genes. Trends Genet 18: 604–606.LD HurstEJ WilliamsC. Pal2002Natural selection promotes the conservation of linkage of co-expressed genes.Trends Genet18604606
- 15. Pal C, Hurst LD (2003) Evidence for co-evolution of gene order and recombination rate. Nat Genet 33: 392–395.C. PalLD Hurst2003Evidence for co-evolution of gene order and recombination rate.Nat Genet33392395
- 16. Williams BA, Slamovits CH, Patron NJ, Fast NM, Keeling PJ (2005) A high frequency of overlapping gene expression in compacted eukaryotic genomes. Proc Natl Acad Sci U S A 102: 10936–10941.BA WilliamsCH SlamovitsNJ PatronNM FastPJ Keeling2005A high frequency of overlapping gene expression in compacted eukaryotic genomes.Proc Natl Acad Sci U S A1021093610941
- 17. Keeling PJ, Slamovits CH (2005) Causes and effects of nuclear genome reduction. Curr Opin Genet Dev 15: 601–608.PJ KeelingCH Slamovits2005Causes and effects of nuclear genome reduction.Curr Opin Genet Dev15601608
- 18. Desportes I, Le Charpentier Y, Galian A, Bernard F, Cochand-Priollet B, et al. (1985) Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J Protozool 32: 250–254.I. DesportesY. Le CharpentierA. GalianF. BernardB. Cochand-Priollet1985Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS.J Protozool32250254
- 19. Notermans DW, Peek R, de Jong MD, Wentink-Bonnema EM, Boom R, et al. (2005) Detection and identification of Enterocytozoon bieneusi and Encephalitozoon species in stool and urine specimens by PCR and differential hybridization. J Clin Microbiol 43: 610–614.DW NotermansR. PeekMD de JongEM Wentink-BonnemaR. Boom2005Detection and identification of Enterocytozoon bieneusi and Encephalitozoon species in stool and urine specimens by PCR and differential hybridization.J Clin Microbiol43610614
- 20. Slamovits CH, Williams BA, Keeling PJ (2004) Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. comb. based on molecular and ultrastructural data. J Eukaryot Microbiol 51: 207–213.CH SlamovitsBA WilliamsPJ Keeling2004Transfer of Nosema locustae (Microsporidia) to Antonospora locustae n. comb. based on molecular and ultrastructural data.J Eukaryot Microbiol51207213
- 21. Thomarat F, Vivares CP, Gouy M (2004) Phylogenetic analysis of the complete genome sequence of Encephalitozoon cuniculi supports the fungal origin of microsporidia and reveals a high frequency of fast-evolving genes. J Mol Evol 59: 780–791.F. ThomaratCP VivaresM. Gouy2004Phylogenetic analysis of the complete genome sequence of Encephalitozoon cuniculi supports the fungal origin of microsporidia and reveals a high frequency of fast-evolving genes.J Mol Evol59780791
- 22. Fast NM, Law JS, Williams BA, Keeling PJ (2003) Bacterial catalase in the microsporidian Nosema locustae: implications for microsporidian metabolism and genome evolution. Eukaryot Cell 2: 1069–1075.NM FastJS LawBA WilliamsPJ Keeling2003Bacterial catalase in the microsporidian Nosema locustae: implications for microsporidian metabolism and genome evolution.Eukaryot Cell210691075
- 23. Mittleider D, Green LC, Mann VH, Michael SF, Didier ES, et al. (2002) Sequence survey of the genome of the opportunistic microsporidian pathogen, Vittaforma corneae. J Eukaryot Microbiol 49: 393–401.D. MittleiderLC GreenVH MannSF MichaelES Didier2002Sequence survey of the genome of the opportunistic microsporidian pathogen, Vittaforma corneae.J Eukaryot Microbiol49393401
- 24. Hinkle G, Morrison HG, Sogin ML (1997) Genes coding for reverse transcriptase, DNA-directed RNA polymerase, and chitin synthase from the microsporidian Spraguea lophii. Biol Bull 193: 250–251.G. HinkleHG MorrisonML Sogin1997Genes coding for reverse transcriptase, DNA-directed RNA polymerase, and chitin synthase from the microsporidian Spraguea lophii.Biol Bull193250251
- 25. Xu J, Pan G, Fang L, Li J, Tian X, et al. (2006) The varying microsporidian genome: existence of long-terminal repeat retrotransposon in domesticated silkworm parasite Nosema bombycis. Int J Parasitol 36: 1049–1056.J. XuG. PanL. FangJ. LiX. Tian2006The varying microsporidian genome: existence of long-terminal repeat retrotransposon in domesticated silkworm parasite Nosema bombycis.Int J Parasitol3610491056
- 26. Kellis M, Patterson N, Endrizzi M, Birren B, Lander ES (2003) Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature 423: 241–254.M. KellisN. PattersonM. EndrizziB. BirrenES Lander2003Sequencing and comparison of yeast species to identify genes and regulatory elements.Nature423241254
- 27. Bennetzen JL, Ramakrishna W (2002) Numerous small rearrangements of gene content, order and orientation differentiate grass genomes. Plant Mol Biol 48: 821–827.JL BennetzenW. Ramakrishna2002Numerous small rearrangements of gene content, order and orientation differentiate grass genomes.Plant Mol Biol48821827
- 28. Coghlan A, Wolfe KH (2002) Fourfold faster rate of genome rearrangement in nematodes than in Drosophila. Genome Res 12: 857–867.A. CoghlanKH Wolfe2002Fourfold faster rate of genome rearrangement in nematodes than in Drosophila.Genome Res12857867
- 29. Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D (2003) Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A 100: 11484–11489.WJ KentR. BaertschA. HinrichsW. MillerD. Haussler2003Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes.Proc Natl Acad Sci U S A1001148411489
- 30. Nilsen F, Endresen C, Hordvik I (1998) Molecular phylogeny of microsporidians with particular reference to species that infect the muscles of fish. J Eukaryot Microbiol 45: 535–543.F. NilsenC. EndresenI. Hordvik1998Molecular phylogeny of microsporidians with particular reference to species that infect the muscles of fish.J Eukaryot Microbiol45535543
- 31. Keogh RS, Seoighe C, Wolfe KH (1998) Evolution of gene order and chromosome number in Saccharomyces, Kluyveromyces and related fungi. Yeast 14: 443–457.RS KeoghC. SeoigheKH Wolfe1998Evolution of gene order and chromosome number in Saccharomyces, Kluyveromyces and related fungi.Yeast14443457
- 32. Zdobnov EM, von Mering C, Letunic I, Torrents D, Suyama M, et al. (2002) Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster. Science 298: 149–159.EM ZdobnovC. von MeringI. LetunicD. TorrentsM. Suyama2002Comparative genome and proteome analysis of Anopheles gambiae and Drosophila melanogaster.Science298149159
- 33. Graham A, Papalopulu N, Krumlauf R (1989) The murine and Drosophila homeobox gene complexes have common features of organization and expression. Cell 57: 367–378.A. GrahamN. PapalopuluR. Krumlauf1989The murine and Drosophila homeobox gene complexes have common features of organization and expression.Cell57367378
- 34. Tamames J (2001) Evolution of gene order conservation in prokaryotes. Genome Biol 2: RESEARCH0020.J. Tamames2001Evolution of gene order conservation in prokaryotes.Genome Biol2RESEARCH0020
- 35. Slamovits CH, Burri L, Keeling PJ (2006) Characterization of a divergent Sec61beta gene in microsporidia. J Mol Biol 359: 1196–1202.CH SlamovitsL. BurriPJ Keeling2006Characterization of a divergent Sec61beta gene in microsporidia.J Mol Biol35911961202
- 36. Burt DW, Bruley C, Dunn IC, Jones CT, Ramage A, et al. (1999) The dynamics of chromosome evolution in birds and mammals. Nature 402: 411–413.DW BurtC. BruleyIC DunnCT JonesA. Ramage1999The dynamics of chromosome evolution in birds and mammals.Nature402411413
- 37. Ranz JM, Casals F, Ruiz A (2001) How malleable is the eukaryotic genome? Extreme rate of chromosomal rearrangement in the genus Drosophila. Genome Res 11: 230–239.JM RanzF. CasalsA. Ruiz2001How malleable is the eukaryotic genome? Extreme rate of chromosomal rearrangement in the genus Drosophila.Genome Res11230239
- 38. Zhang Q, Singh I, Sheoran A, Feng X, Nunnari J, et al. (2005) Production and characterization of monoclonal antibodies against Enterocytozoon bieneusi purified from rhesus macaques. Infect Immun 73: 5166–5172.Q. ZhangI. SinghA. SheoranX. FengJ. Nunnari2005Production and characterization of monoclonal antibodies against Enterocytozoon bieneusi purified from rhesus macaques.Infect Immun7351665172
- 39. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed.: Cold Spring Harbor Laboratory Press. J. SambrookEF FritschT. Maniatis1989Molecular cloning: a laboratory manual, 2nd ed.: Cold Spring Harbor Laboratory Press.
- 40. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403–410.SF AltschulW. GishW. MillerEW MyersDJ Lipman1990Basic local alignment search tool.J Mol Biol215403410
- 41. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.SF AltschulTL MaddenAA SchafferJ. ZhangZ. Zhang1997Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.Nucleic Acids Res2533893402