The p24 family is thought to be somehow involved in endoplasmic reticulum (ER)-to-Golgi protein transport. A subset of the p24 proteins (p24α3, -β1, -γ3 and -δ2) is upregulated when Xenopus laevis intermediate pituitary melanotrope cells are physiologically activated to produce vast amounts of their major secretory cargo, the prohormone proopiomelanocortin (POMC).
Here we find that transgene expression of p24α3 or p24δ2 specifically in the Xenopus melanotrope cells in both cases causes an effective displacement of the endogenous p24 proteins, resulting in severely distorted p24 systems and disparate melanotrope cell phenotypes. Transgene expression of p24α3 greatly reduces POMC transport and leads to accumulation of the prohormone in large, ER-localized electron-dense structures, whereas p24δ2-transgenesis does not influence the overall ultrastructure of the cells nor POMC transport and cleavage, but affects the Golgi-based processes of POMC glycomaturation and sulfation.
Transgenic expression of two distinct p24 family members has disparate effects on secretory pathway functioning, illustrating the specificity and non-redundancy of our transgenic approach. We conclude that members of the p24 family furnish subcompartments of the secretory pathway with specific sets of machinery cargo to provide the proper microenvironments for efficient and correct secretory protein transport and processing.
Citation: Strating JRPM, Bouw G, Hafmans TGM, Martens GJM (2007) Disparate Effects of p24α and p24δ on Secretory Protein Transport and Processing. PLoS ONE 2(8): e704. doi:10.1371/journal.pone.0000704
Academic Editor: Raphael Valdivia, Duke University Medical Center, United States of America
Received: May 25, 2007; Accepted: July 2, 2007; Published: August 8, 2007
Copyright: © 2007 Strating et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grant 811.38.002 from The Netherlands Organisation for Scientific Research-Earth and Life Sciences (NWO-ALW).
Competing interests: The authors have declared that no competing interests exist.
The secretory pathway consists of a number of distinct membrane-bounded subcompartments that have specialized functions in the process of protein biosynthesis . Proteins that pass through the subcompartments undergo various posttranslational modifications (e.g. glycosylation, sulfation and proteolytic cleavage) that are essential for their biological activity. The biosynthetic process includes the selective packaging of proteins from the endoplasmic reticulum (ER) into vesicles for delivery to the subcompartments. Members of the p24 family of type-I transmembrane proteins are thought to be somehow involved in the trafficking events between the ER and the Golgi .
The p24 proteins have been found to be abundantly present in transport vesicles coated with the COPI- or COPII-coat protein complex as well as in ER, intermediate compartment and cis-Golgi membranes, whereby they shuttle constantly between these subcompartments (reviewed in , ). The ∼24K p24 proteins constitute a family that can be subdivided into four subfamilies (p24α, β, γ and δ) , with each family member displaying multiple domains conserved from yeast to mammals (reviewed in ). In view of their structural resemblance, the various members of the p24 family likely have similar functions. The p24 proteins have been primarily proposed to act as receptors for specific sets of secretory cargo molecules (cargo receptor model) . In this report, “secretory cargo” refers to the biologically active transmembrane and soluble proteins that are transported to the plasma membrane or extracellular matrix as well as to the bioactive soluble proteins that are secreted into the extracellular space, whereas the machinery proteins or lipids that are supplied to subcompartments of the secretory pathway to provide the proper microenvironments for efficient and correct transport and processing of the secretory cargo are designated “machinery cargo”. Studies in yeast lacking one or more p24 proteins have indeed indicated that p24 plays a role in the anterograde transport of some (Gas1p and invertase), but not all (α-factor, acid phosphatase, carboxypeptidase Y, alkaline phosphatase and Gap1p) secretory cargo –. Furthermore, when injected into mammalian cells in culture antibodies against the cytoplasmic tail of p23 (p24δ1) inhibited the transport of the secretory cargo protein VSV-G . Based on a variety of functional studies, alternative roles for p24 have been proposed, including its involvement in the biogenesis and proper functioning of transport vesicles and in the organization of membranes of the secretory pathway –. Surprisingly, an octuple yeast knock-out strain lacking all p24 proteins was viable , whereas homozygous p23 (p24δ1) knock-out mice died early in embryonic development . Thus, studies on the role of p24 in the early secretory pathway have not provided conclusive answers.
In general, the outcome of a functional study is greatly determined by the availability of a well-defined model system. To explore the role of p24 proteins, we therefore decided to use the intermediate pituitary melanotrope cells of the amphibian Xenopus laevis as a cell model to study protein transport in a physiological context. The Xenopus melanotrope cells produce α-melanophore-stimulating hormone (α-MSH), which mediates the process of background adaptation of the animal. The biosynthetic and secretory activity of the melanotrope cells can be modulated by placing the frogs on a white (inactive melanotrope cells) or black (highly active melanotrope cells) background. The manipulation of the activity of the melanotrope cells is strictly regulated by inhibitory and stimulatory neurons of hypothalamic origin. Upon activation, the melanotrope cells produce and proteolytically cleave vast amounts of the prohormone proopiomelanocortin (POMC), the precursor of a number of bioactive peptides, including α-MSH (reviewed in ). In the activated melanotrope cells, proteins upregulated together with POMC are thought to play a role in the biosynthesis of the prohormone  and include a subset of p24 proteins, namely p24α3, p24β1, p24γ3 and p24δ2. Two other members of the p24 family (p24γ2 and p24δ1) are expressed in the melanotrope cells as well, but not coordinately with POMC . To examine the role of p24 in the biosynthesis of POMC, we chose to generate and analyze Xenopus lines with transgene expression of p24α3 or p24δ2, i.e. two p24s of the upregulated set. We used a POMC gene promoter fragment to target the expression of the transgenes specifically to the melanotrope cells , leaving the regulation of these cells by hypothalamic neurons intact. We find that the p24α3- and p24δ2-trangenic frogs have distinct melanotrope cell phenotypes in that POMC transport and processing was differently affected, allowing us to conclude that p24α3 and p24δ2 have non-redundant roles in maintaining the functional and structural integrity of the secretory pathway.
Generation of Xenopus laevis with stable transgene expression of p24α3 or p24δ2 specifically in the melanotrope cells
For our functional studies on p24, we generated Xenopus laevis transgenic for the p24α3 or the p24δ2 protein. In order to drive transgene expression specifically to the melanotrope cells of the Xenopus intermediate pituitary, we made DNA-constructs containing a 529-bp Xenopus laevis POMC gene A promoter fragment (pPOMC ) in front of Xenopus laevis p24α3 or p24δ2 cDNA. To allow direct selection of embryos expressing the transgene, we fused GFP to the C-terminus of p24α3 and p24δ2. The linearised DNA-constructs (pPOMC-p24α3-GFP, Figure 1A or pPOMC-p24δ2-GFP, Figure 1B) were mixed with wild-type Xenopus sperm nuclei and the mixtures were microinjected into unfertilized wild-type Xenopus eggs. We generated three independent transgenic F0 frogs for p24α3-GFP (#55, #602 and #605) and four independent transgenic F0 animals for p24δ2-GFP (#115, #124, #125 and #224). Next, F1 offspring was produced by in vitro fertilization of eggs harvested from wild-type females with sperm isolated from the testes of individual transgenic males or by in vitro fertilization of eggs harvested from individual transgenic females with sperm isolated from the testes of wild-type males. Expression of the transgenes specifically in the intermediate pituitary could readily and directly be observed in living tadpoles (Figure 1C) and in adult frogs after lifting the brain (Figure 1D).
(A and B) Schematic representation of the linear injection fragments pPOMC-p24α3-GFP (A) and pPOMC-p24δ2-GFP (B) containing a Xenopus POMC gene promoter fragment (pPOMC) and the protein-coding sequence of p24α3-GFP (transgenic lines #605, #55 and #602) or p24δ2-GFP (lines #125, #115, #124 and #224); pPOMC drives transgene expression specifically to the melanotrope cells. (C) Pituitary-specific GFP-fluorescence (arrows) in living tadpoles transgenic for p24α3 (line #55) or p24δ2 (line #224); G, gut; E, eye; N, nose. (D) Fluorescence in the intermediate lobe (IL) and not in the anterior lobe (AL) of the pituitary of adult frogs transgenic for p24α3 (#55) or p24δ2 (#224).
We (in line #124 p24δ2-transgenic Xenopus ) and others (in heterozygous p24 knock-out mice , by p24 knock-down in mammalian cells in culture ,  and in p24 knock-out yeast , ) have observed that manipulation of the expression of a single p24 family member interferes with the endogenous p24 system and affects the steady-state levels of the other p24 family members. We therefore studied in the melanotrope cells of the three p24α3- and four p24δ2-transgenic Xenopus lines the steady-state levels of members of the endogenous p24 protein family. In the melanotrope cells of the three lines transgenic for the p24α3-GFP fusion protein, the transgene product effectively displaced the endogenous p24α3, β1, γ3, δ1 and δ2 proteins (75–90% displacement; n = 4; Figure 2A and data not shown). We selected line #55 to further study the effect of p24α3 transgene expression on the functioning of the melanotrope cells. In the transgenic melanotrope cells of line #224, the p24δ2-GFP fusion protein displaced the endogenous p24 proteins similarly effective (70–98% displacement; n = 4; Figure 2A). This degree of displacement was higher than that in the previously described p24δ2-transgenic melanotrope cells of lines #115, #125 and #124 . We therefore decided to select p24δ2-transgenic line #224 for further functional analysis. Surprisingly, whereas the level of expression of the p24α3-GFP fusion protein was ∼6 times lower than that of p24δ2-GFP (n = 11; Figure 2B), the p24α3 and p24δ2 fusion proteins were similarly effective in displacing the endogenous p24 proteins.
(A and B) Western blot analysis of neurointermediate lobe lysates from wild-type frogs (wt) and frogs transgenic for p24α3 (#55) or p24δ2 (#224) with anti-p24 antibodies (A) or an anti-GFP antibody (B). Tubulin was used as a control for equal loading. (C and D) Immuno-electron microscopy analysis of intermediate pituitary melanotrope cells from frogs transgenic for p24α3 (#55; C) or p24δ2 (#224; D1 and D2) using an anti-GFP antibody. The p24α3-transgene product was mainly present in ER-localized electron-dense structures (EDS; C), whereas the p24δ2-transgene product was found on the ER (D1) and on the Golgi (D2). G, Golgi; ER, endoplasmic reticulum. Bars equal 200 nm.
We next investigated the localization of the fusion proteins within the transgenic melanotrope cells by immuno-electron microscopy (IEM) using an anti-GFP antibody. In the p24α3-transgenic melanotrope cells, the majority of the transgene product was found in ER-localized electron-dense structures (EDS, Figure 2C) in which we also observed POMC (see below). In the p24δ2-transgenic cells, the gold-label was detected mostly on the ER and the Golgi (Figure 2D), indicating cycling of the fusion protein between these compartments and in line with our previous observation of ER/Golgi localization of the transgene product in line #124 p24δ2–transgenic melanotrope cells  and consistent with the localization of endogenous p24 proteins (reviewed in ).
Together, the studies on the steady-state p24 protein levels showed that in the p24α3- and p24δ2-transgenic melanotrope cells the entire set of endogenous p24 proteins was nearly completely displaced by the transgene product and therefore the fusion protein was essentially the only p24 protein available to the transgenic cells.
Steady-state levels of secretory cargo proteins in the transgenic Xenopus melanotrope cells
To study the effect of the transgene expression of p24α3 or p24δ2 on the functioning of the secretory pathway, we first examined the steady-state levels of the soluble secretory cargo proteins POMC and its processing enzyme prohormone convertase PC2 in the transgenic melanotrope cells. Western blot analysis revealed that the levels of the 37K POMC prohormone were significantly increased in the p24α3-transgenic cells (∼11-fold; n = 6), whereas in the p24δ2-transgenic cells the steady-state levels of 37K POMC were not affected (n = 6; Figures 3). Furthermore, the proenzyme form of PC2 was detected only in the p24α3-transgenic melanotrope cells, and not in wild-type and the p24δ2-transgenic cells (Figure 3). We then studied the steady-state levels of a transmembrane cargo protein, namely of the type-I transmembrane protein amyloid-β precursor protein (APP). Xenopus APP is synthesized as an ∼100K N-linked glycosylated protein that in the Golgi is converted into an ∼110K O-glycosylated mature protein . Western blot analysis using anti-APP antibodies showed in wild-type cells an ∼5.4-fold (n = 4) higher amount of 110K APP than the level of immature 100K APP. In the p24α3-transgenic cells, the level of immature 100K APP was clearly increased (∼1.9-fold; n = 4), such that the ratio between 110K and 100K APP was ∼2.8 (n = 4), whereas the ratio between both forms as well as the total amount of APP were not significantly affected in the p24δ2-transgenic cells (n = 4; Figure 3). Thus, increased steady-state levels of the unprocessed forms of both soluble (POMC, pro-PC2) and transmembrane (APP) cargo proteins were detected in the p24α3-, but not in the p24δ2-, transgenic melanotrope cells. Unfortunately, the use of a battery of antibodies against multiple mammalian ER and Golgi enzymes and matrix proteins did not allow us to determine the steady-state levels of these proteins in the transgenic cells because of the inability of the antibodies to cross-react with the Xenopus orthologs.
Western blot analysis of neurointermediate lobe (NIL) lysates from wild-type frogs (wt) and frogs transgenic for p24α3 (#55) or p24δ2 (#224) using antibodies directed against the soluble cargo proteins proopiomelanocortin (POMC) and prohormone convertase 2 (PC2), and the transmembrane cargo amyloid-β precursor protein (APP). Tubulin was used as a control for equal loading.
We next investigated the peptides produced by POMC cleavage through matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS). The POMC-derived peptides des-N-α-acetyl-α-MSH (the nonacetylated form of α-MSH), α-MSH, γ1-MSH, β-MSH and two corticotrophin-like intermediate lobe peptides (CLIP A and B) were found in wild-type melanotrope cell extracts as well as in samples from the p24α3- and p24δ2-transgenic cells (data not shown), indicating that both the p24α3- and the p24δ2-transgenic melanotrope cells converted 37K POMC into the same set of bioactive peptides as the wild-type cells.
Biosynthesis of POMC in the transgenic Xenopus melanotrope cells
In view of the observed differences in the steady-state protein levels, we next wondered whether the biosynthetic activity for POMC production was affected in the transgenic melanotrope cells. From the pituitary (consisting of the pars nervosa, and the anterior and intermediate lobes), the anterior part can be dissected but the pars nervosa is intimately associated with the intermediate pituitary (the neuroendocrine melanotrope cells). For our studies, we therefore used the neurointermediate lobe (NIL; pars nervosa plus intermediate lobe) of the pituitary to study the biosynthetic events in the melanotrope cells. Since the Xenopus pars nervosa consists of biosynthetically inactive nerve terminals of hypothalamic origin, the radiolabelled proteins are synthesized exclusively by the melanotrope cells of the intermediate pituitary. Metabolic cell-labeling experiments revealed that following a 30-min pulse the patterns of newly synthesized proteins produced in the p24α3- and in the p24δ2-transgenic melanotrope cells were similar to that in wild-type cells (data not shown). Thus, protein biosynthesis was not affected in the transgenic melanotrope cells. Following a 30-min pulse and a 3-hr chase period, we observed a clearly higher amount of newly synthesized 37K POMC remaining in the p24α3-transgenic than in wild-type cells (∼5-fold; n = 10; Figures 4A and 4B). In contrast, following the pulse-chase incubation the amount of newly synthesized 37K POMC found in the p24δ2-transgenic melanotropes was similar to that in wild-type cells (n = 6; Figures 4A and 4B). These observations are in line with the Western blot results showing higher steady-state levels of 37K POMC in the p24α3-transgenic melanotrope cells than in wild-type and the p24δ2-transgenic cells (Figure 3). Thus, the levels of POMC biosynthesis were not changed in the melanotrope cells of the two transgenic lines, but the extent of POMC processing appeared to be affected in the p24α3-transgenic cells.
(A–C) Neurointermediate lobes (NILs) from wild-type frogs (wt) and frogs transgenic for p24α3 (#55) or p24δ2 (#224) were pulse labeled with [35S]-Met/Cys for 30 min and subsequently chased for 3 hrs. Newly synthesized proteins extracted from the NILs (Cells; 5% of extract) and secreted into the incubation medium (Media; 20%) were resolved by 15% SDS-PAGE and visualized by autoradiography. (A) The analysis was performed in six independent experiments and a representative autoradiogram is shown. (B) The amount of newly synthesized 37K POMC in wild-type (n = 16) and the p24α3-transgenic (n = 10) and p24δ2-transgenic (n = 6) cells was quantified and is shown relative to the wild-type cells. (C) The amounts of newly synthesized 18K and 18K* POMC in wild-type (n = 12) and the p24α3-transgenic (n = 5) and p24δ2-transgenic (n = 6) cells were quantified and are shown relative to wild-type 18K POMC. Indicated are the 18K/18K* ratios and their statistical evaluations. Data are shown as means +/− SEM. n.s., not significant; **, p<0.01; ***, p<0.001.
Processing of newly synthesized POMC in the transgenic Xenopus melanotrope cells
Because of the observed accumulation of POMC in the p24α3-transgenic melanotrope cells, we next studied the dynamics of POMC processing by performing pulse-chase metabolic cell labeling experiments. The first endoproteolytic cleavage of newly synthesized 37K POMC yields an ∼18K POMC cleavage product that represents the N-terminal portion of 37K POMC and contains the only N-linked glycosylation site present in the Xenopus POMC molecule . In wild-type melanotrope cells, after a 30-min pulse and 3-hr chase period, two forms of 18K POMC are produced, a major product (∼80%) indicated as 18K POMC and a minor (∼20%), slightly slower migrating product that we previously designated 18K* POMC (Figure 4A; ). In the p24α3-transgenic cells, the amounts of the 18K and the 18K* POMC processing products were clearly reduced (n = 6; Figures 4A and 4C), indicating less proteolytic processing of newly synthesized 37K POMC. In the p24δ2-transgenic cells, the level of 18K POMC was greatly diminished (∼7-fold; n = 6), whereas the amount of the 18K* POMC product was clearly increased (∼3-fold; n = 6; Figures 4A and 4C), reducing the ratio 18K/18K* POMC from ∼4 in wild-type cells to ∼0.2 in the p24δ2-transgenic cells (Figure 4C). In line #124 p24δ2-transgenic cells, we have previously also observed a decrease in the 18K/18K* POMC ratio, albeit less pronounced . The two forms of 18K POMC presumably differ in the sugar moieties added to their N-glycosylation sites, because deglycosylation of the newly synthesized melanotrope cell proteins with peptidyl N-glycosidase F (PNGaseF), an enzyme that removes all N-linked sugar groups from the protein backbone, caused a shift of both 18K and 18K* POMC to an ∼15.5K product (Figure 5). These results suggest that only in the p24δ2-, and not in the p24α3-, transgenic cells the glycosylation machinery was affected. Next, HPLC analysis was used to study the newly synthesized peptides resulting from the proteolytic cleavage of radiolabelled POMC. This analysis revealed that the proteolytic cleavage of newly synthesized POMC to des-α-MSH, α-MSH, the two forms of CLIP and the two forms of endorphin was similar in the transgenic and wild-type cells (data not shown), in line with the results obtained by mass spectrometry analysis of the steady-state peptides. Together, the biosynthetic studies on the processing of newly synthesized POMC showed that the processing rate was severely reduced in the p24α3-transgenic cells and that the glycosylation event was affected in the p24δ2-transgenic cells.
Neurointermediate lobes (NILs) from wild-type frogs (wt) and frogs transgenic for p24α3 (#55) or p24δ2 (#224) were pulse labeled with [35S]-Met/Cys for 30 min and subsequently chased for 3 hrs. Newly synthesized proteins extracted from the NILs were deglycosylated with PNGaseF (F) or control-treated (C), resolved by 20% SDS-PAGE and visualized by autoradiography; the #55 lanes were exposed three times longer than the other lanes.
Sulfation of newly synthesized POMC in the transgenic Xenopus melanotrope cells
In addition to N-linked glycosylation and proteolytic cleavage, POMC is posttranslationally modified by sulfation in the trans-Golgi network (TGN) of the Xenopus melanotrope cells . To examine whether sulfation of newly synthesized POMC was affected in the transgenic melanotrope cells, we pulse labeled the cells in the presence of both 3H-lysine and 35S-sulfate. The labeling with 3H-lysine allowed us to quantify the total amount of newly synthesized POMC and 35S-sulfate labeling revealed the degree of sulfation of the newly synthesized prohormone. 37K POMC sulfation was not affected in the p24α3-transgenic cells, whereas we observed an ∼5-fold increase in the sulfation of the prohormone in the p24δ2-transgenic cells (Figure 6). Removal of the N-linked glycans from the sulfate-labeled 37K POMC with PNGaseF increased its electrophoretic mobility but did not affect the degree of sulfate labeling (data not shown), suggesting that the sulfate did not reside on the glycogroup. Together with the fact that the 18K POMC species were not sulfated and in line with the presence of a predicted tyrosine sulfation site in the C-terminal half of the Xenopus laevis 37K POMC molecule (Sulfinator prediction program ), the sulfate label presumably resided on tyrosine residue 188 (SLELDY188PEIDLDEDIED). The biosynthetic studies with 35S-sulfate thus showed that the level of POMC sulfation in the TGN was increased in the p24δ2-, but not the p24α3-, transgenic melanotrope cells.
Neurointermediate lobes (NILs) from wild-type frogs (wt) and frogs transgenic for p24α3 (#55) or p24δ2 (#224) were pulse labeled with 35S-sulfate and 3H-lysine for 15 min. Newly synthesized proteins extracted from the NILs were resolved by 15% SDS-PAGE and the amount of [35S]SO4 and 3H-lysine incorporated into newly-synthesized 37K POMC was determined. Shown are the amounts of newly synthesized sulfated 37K POMC produced in the transgenic relative to wt NILs. Data are shown as means +/− SEM (wt, n = 7; transgenics, n = 5). *, p<0.05; n.s., not significant.
Electron and immuno-electron microscopy analysis of the transgenic Xenopus melanotrope cells
In view of the observed differences in POMC transport and processing, we decided to examine the ultrastructure of the transgenic cells by performing EM. Like in wild-type cells, in the p24α3- and p24δ2-transgenic melanotrope cells the rough ER was well developed. However, the p24α3-transgenic cells contained besides the large spherical EDS a Golgi apparatus that appeared as fragmented mini-stacks (Figures 7A and 7B). Despite the fact that the p24 system was also severely distorted, the p24δ2-transgenic cells had normal Golgi ribbons and overall no gross morphological differences were observed between these cells and wild-type melanotropes (Figures 7A and 7C). IEM using the anti-POMC antibody revealed in the p24α3-transgenic cells strong immunolabeling for POMC in the EDS that were localized within the ER lumen (Figure 7E), indicating that the unprocessed and accumulated prohormone most likely resided in these structures. Furthermore, POMC-labeling was found in dense-core secretory granules of wild-type and the p24α3- and p24δ2-transgenic melanotrope cells (Figures 7D–F). Together, these results showed that the expression of the p24α3-transgene product caused an accumulation of POMC in large electron-dense structures within the ER and impaired the structural integrity of the Golgi apparatus, whereas the transgenic expression of p24δ2 did not affect the ultrastructure of the cells.
(A–C) Wild-type (wt; A1 and A2) intermediate pituitary cells showed a well-developed rough endoplasmic reticulum and extensive Golgi-ribbons. The p24α3-transgenic cells (#55; B1 and B2) contained Golgi mini-stacks and large electron-dense structures (EDS). The p24δ2-transgenic cells (#224; C1 and C2) showed an ultrastructure similar to that of wild-type cells. The dotted lines highlight the outline of the Golgi. (D–F) Immuno-electron microscopy analysis of intermediate pituitary melanotrope cells from wt frogs (D), and frogs transgenic for p24α3 (#55; E) or p24δ2 (#224; F) using an anti-POMC antiserum. Immunoreactivity was found in dense-core secretory granules (filled arrowheads) in wt and p24α3- and p24δ2-transgenic cells and occasionally in newly forming secretion granules still attached to the trans-Golgi network (open arrowheads). In addition, in the p24α3-transgenic cells a strong POMC-immunolabeling was observed in the EDS, which were localized to the ER lumen (arrow) and occasionally in EDS newly forming within the ER lumen (open arrow). G, Golgi; L, lysosome; M, mitochondrion; N, nucleus; RER, rough endoplasmic reticulum; PM, plasma membrane; sg, immature secretory granules. Bars equal 1 µm (A–C); 500 nm (D–F).
The type-I transmembrane p24 proteins are abundantly present in ER- and Golgi-derived transport vesicles, and are therefore thought to play an important role in some aspect of cargo-selective transport through the early secretory pathway (reviewed in ). The complex and dynamic behavior of this protein family has hampered functional analyses. In this study, we used the Xenopus laevis intermediate pituitary melanotrope cells to explore the function of p24. The Xenopus melanotrope cells are well-defined neuroendocrine cells of which the biosynthetic and secretory activity can be physiologically induced by placing the frogs on a black background (for review, see ). Upon black-background adaptation, four p24 family members (p24α3, p24β1, p24γ3 and p24δ2), but not p24γ2 and p24δ1, are upregulated in the melanotrope cells together with the main secretory cargo protein POMC . The specific upregulation of these four p24 proteins suggests that they are somehow involved in the biosynthesis of POMC. Of the four upregulated p24 proteins, we chose p24α3 and p24δ2 for our functional analyses and generated three independent stable transgenic lines expressing p24α3 and four independent stable p24δ2-transgenic lines, using a POMC gene promoter fragment  to drive transgene expression specifically to the melanotrope cells.
We showed in the transgenic Xenopus melanotrope cells a nearly complete displacement of the endogenous p24α3, p24β1, p24γ3, p24δ1 and p24δ2 proteins by both the p24α3- and the p24δ2-transgene product. This displacement effect is not in line with the result of the transient overexpression of TMP21 (p24δ1) or p24a (p24β1) in cultured tumor cells, which did not influence the steady-state levels of p24a or TMP21 respectively . This apparent discrepancy may have been caused by the transient nature of the overexpression in the tumor cells (in our transgenic system the overexpression was stable). To our knowledge, the effect of stable overexpression of an exogenous p24 protein on the endogenous p24 system has not been studied before. Yet, knockout or knockdown of a p24 member in yeast or mammalian cells has led to a reduction in the levels of other p24 proteins as well , , . At present, we can only speculate why the endogenous p24 proteins were displaced in the p24α3- and p24δ2-transgenic Xenopus melanotrope cells. For instance, the amount of early secretory pathway membranes that can harbor p24 proteins may be limited and the relative levels of the various newly synthesized p24 family members expressed in a cell will then determine the final composition of the p24 system. In the transgenic melanotrope cells, the relatively high amount of newly synthesized transgene product will occupy most of the available space, consequently leading to the displacement of the endogenous p24 proteins. Alternatively, since p24 protein stability depends on the presence of other p24 proteins and their interaction within p24 complexes , , , and the stable transgene expression in the Xenopus melanotrope cells disturbed the stoichiometry of the p24 system, the transgenic manipulation may well have resulted in altered p24 complex compositions. As a consequence, the endogenous p24 proteins would not be able to form their favored and thus stable complexes, leading to the observed reduced endogenous p24 protein levels. Remarkably, for unknown reasons the p24α3-transgene product displaced the endogenous p24 proteins more effectively than the p24δ2-transgene product.
Irrespective of the explanation, the nearly complete displacement of the endogenous p24 proteins implies that in the p24α3- and p24δ2-transgenic Xenopus melanotrope cells the p24 system consists essentially of only the p24α3- and p24δ2-transgene product respectively. This unique situation allowed us to search for the role of p24 by analyzing the functioning of the secretory pathway in the transgenic cells. For our functional analysis, we took advantage of the fact that the Xenopus melanotrope cells produce and process only one major secretory cargo protein (POMC) and that in wild-type melanotrope cells the biosynthetic processes have been previously analyzed in detail (reviewed in ). In the p24α3-transgenic melanotrope cells, the amounts of newly synthesized POMC, the level of POMC sulfation as well as the ratio of 18K- to 18K*-POMC were normal, but the rates of transport and proteolytic cleavage of 37K POMC were greatly reduced. The uncleaved POMC accumulated in large electron-dense structures localized to the ER. Since these structures were present only in the p24α3- and not in the p24δ2-transgenic melanotropes nor in Xenopus melanotropes transgenic for other (transmembrane) proteins (our unpublished observations), they were not merely a result of transgenic protein expression per se. Furthermore, in the p24α3-transgenic cells the Golgi appeared as ministacks, whereas in wild-type and the p24δ2-transgenic cells this subcompartment was present as Golgi ribbons. An attractive explanation for these observations is that the excess of p24α3 had affected the supply of the machinery cargo that is required for a correct microenvironment in the early secretory pathway with the consequence of an improper transport and accumulation of POMC, and an abnormal structural organization of the Golgi apparatus in the p24α3-transgenic cells. In the p24δ2-transgenic melanotrope cells, the overall ultrastructure was not affected by the transgenic manipulation and the levels and rates of POMC synthesis, transport and cleavage were normal. Also, the steady-state and biosynthetic levels of the POMC-cleavage enzyme PC2 and the transmembrane cargo APP were similar in wild-type and the p24δ2-transgenic cells. However, higher amounts of the differentially glycosylated 18K* POMC-product and of sulfated POMC were found in the p24δ2-transgenic than wild-type or the p24α3-transgenic cells. Since the processes of glycomaturation and sulfation occur in the Golgi, it appears that excess p24δ2 had resulted in an increased supply of the machinery cargo that is responsible for these processes in this subcompartment of the secretory pathway. Taken together, our results suggest that in the p24α3- and p24δ2-transgenic melanotrope cells the microenvironments of, albeit different, subcompartments in the early stages of the secretory pathway were affected, pointing to a role for p24 proteins in the transport of subsets of machinery cargo and thus in furnishing specific subcompartments of the early secretory pathway.
In view of their structural similarity, the various p24 family members presumably have similar functions. Thus far, the p24 proteins have been mainly implicated in the selection and packaging of secretory cargo proteins (cargo receptor model; reviewed in ). However, if the p24 proteins were receptors for secretory cargo, it is not clear why in the Xenopus melanotrope cells four p24 proteins (p24α3, p24β1, p24γ3, p24δ2) are coordinately expressed with POMC, the major secretory cargo (the prohormone represents >80% of all newly synthesized proteins; ). Furthermore, if the four upregulated p24 proteins would function together as a heterotetrameric POMC receptor, one would not have expected to observe such different phenotypes for the p24α3- and p24δ2-transgenic melanotrope cells. Reasoning along the same lines, the role suggested for p24 in vesicle biogenesis  appears to be improbable. Thus, the results of our study do not support a role for p24 in secretory cargo receptor or vesicle functioning, but rather are most consistent with a function in furnishing secretory pathway subcompartments. Our furnishing model is compatible with the results of other studies in which the expression of p24 was also manipulated. First, overexpressing p23 (p24δ1) or a p25 (p24α2) mutant protein in cultured mammalian tumor cells resulted in the formation of Golgi mini-stacks and highly specialized lipid subdomains, respectively, leading the investigators to conclude that p24 proteins are involved in the formation of membrane subdomains in the early secretory pathway , . Second, antibodies against the cytoplasmic tails of p24α2, p23 (p24δ1) or p24 (p24β1) inhibited the formation of transport carriers and cargo transport , , , consistent with a role for p24 in the supply of machinery cargo to ER-subdomains that are capable of forming transport carriers. Third, reducing the expression of Tmp21/p23 (p24δ1) in mammalian cells in culture with siRNA led to an effect on the fate of the secretory cargo , . Fourth, the finding that in single and multiple yeast p24 knock-outs the vesicular incorporation and maturation of a specific set of secretory cargo proteins was reduced –, ,  is reminiscent of what we observed in the p24α3-transgenic cells and may thus also be explained by an improper supply of subsets of machinery cargo involved in transport carrier formation or maturation of secretory cargo. In addition, these yeast knock-outs had defects in the retention of the ER-chaperones Kar2p (BiP) and protein disulfide isomerase, which may have been caused by an improper maintenance of the ER-retrieval machinery , , , . Fifth, the injection of antibodies against the cytoplasmic-tail of p23 (p24δ1) into cultured mammalian tumor cells inhibited retrograde cargo transport due to a malfunctioning of the ER-retrieval machinery . Indeed, p23 (p24δ1) directly interacted with the KDEL-receptor, a component of the retrieval system . Sixth, manipulation of p23 (p24δ1) or p25 (p24α2) expression levels in cultured mammalian tumor cells affected the ER localization of the phosphatase machinery protein TC48 . Seventh, in C. elegans mutated p24 proteins damaged the supply of machinery cargo involved in the quality control system, allowing the transport of a mutant cargo protein out of the ER . Thus, the phenotypes displayed by the various cell types in which p24 expression had been manipulated are consistent with a role for p24 in the supply of machinery cargo to secretory pathway subcompartments. Such a role is supported by the results of our search of the BIOGRID database  for physical interactors with yeast p24 proteins revealing that, besides constituents of the COPI- and COPII-coat machinery, a substantial number of the interactors are components of the secretory pathway machinery, like membrane-lipid-synthesizing proteins and N- and O-glycosylation enzymes.
An intriguing extension of our model on the role of p24 concerns the recently proposed mechanism for the anterograde-directed bulk flow transport of secretory cargo with progressive en bloc protrusion from the ER, leading to the formation of large tubulo-saccular structures that transport the cargo to the Golgi apparatus . In the so-called ER-exit sites (ERES) adjacent to the large cargo carriers, the COPII-system would recruit machinery cargo needed to create a microenvironment in the ER and Golgi (e.g. a locally different pH or Ca2+-concentration) that facilitates efficient secretory cargo delivery to the newly forming transport carriers, and allows later trafficking and processing events . For the Xenopus melanotrope cells, an attractive model for ER-to-Golgi secretory cargo transport may therefore involve an en bloc bulk-flow protrusion of POMC with each of the upregulated p24 family members recruiting a specific set of machinery cargo to the ERES in order to allow efficient transport and subsequent correct processing of POMC in the regulated secretory pathway of these neuroendocrine cells. Our model differs from the current models proposing a role for p24 as receptor for secretory cargo or in vesicle biogenesis, and will serve as a challenging foundation for future p24-research.
In summary, we explored the role of two p24 family members by applying a stable, cell-specific transgenic approach in a well-defined and physiologically relevant neuroendocrine cell model. Since both the p24α3- as well as the p24δ2-transgenic melanotrope cells showed a nearly complete knockdown of the endogenous p24 proteins and still displayed disparate phenotypes, the observed effects are specific and caused by the transgene products rather than by the absence of the endogenous p24 proteins, indicating the selectivity and non-redundancy of our approach. We conclude that in the p24α3-melanotrope cell phenotype an improper furnishing of machinery cargo to the early stages of the secretory pathway hampered transport of secretory cargo from the ER, whereas in the p24δ2-phenotype the supply of a different set of machinery cargo influenced post-translational secretory cargo modifications in the Golgi. Collectively, the results are thus most consistent with a role for p24 in furnishing the various subcompartments of the early secretory pathway with specific sets of machinery cargo in order to create the proper microenvironments for efficient and correct secretory cargo transport and processing to eventually generate bioactive proteins that function in the plasma membrane/extracellular matrix or are secreted into the extracellular space as biological signals.
Materials and Methods
South African claw-toed frogs Xenopus laevis were bred and reared in the Central Animal Facility of the Radboud University (Nijmegen, The Netherlands). Animals were adapted to a black background for at least three weeks. All animal experiments were carried out in accordance with the European Communities Council Directive 86/609/EEC for animal welfare, permit RBD0166(H10) to generate and house transgenic Xenopus and permits RU-DEC 2003-53 and 2007-027 from the animal experiment committee of the Radboud University for the use of Xenopus laevis frogs.
The rabbit polyclonal antibodies against a region in the lumenal part of Xenopus laevis p24α3, against the C-terminal tail of Xenopus p24γ3, against part of the lumenal region of Xenopus p24δ1 (anti-RH6), against portions of the lumenal and C-terminal regions of Xenopus laevis p24δ2 (anti-1262N and anti-1262C respectively) and against the C-terminal region of Xenopus APP (C87) have been described previously , , . We raised a polyclonal antibody against Xenopus p24β1 by injecting rabbits with a recombinant protein encompassing the lumenal domain of Xenopus p24β1 fused to GST. A polyclonal antibody against GFP was obtained from Dr. J. Fransen (NCMLS, Nijmegen, The Netherlands), against Xenopus POMC (ST62, recognizing only the precursor form) from Dr. S. Tanaka (Shizuoka University, Japan), against recombinant mature human PC2 from Dr. W.J.M. Van de Ven (University of Leuven, Belgium) and the monoclonal anti-tubulin antibody E7 was obtained from Dr. B. Wieringa (NCMLS, Nijmegen, The Netherlands) .
Generation of Xenopus laevis transgenic for p24α3 or p24δ2
For the Xenopus transgenesis experiments, eggs were harvested from mature wild-type female Xenopus injected with 375IU human chorionic gonadotropin hormone (Pregnyl; Organon, Oss, The Netherlands) 18hrs before. The eggs were collected in 1×MMR (0.1M NaCl, 2mM KCl, 1 mM MgCl2, 1.5mM CaCl2, 5mM HEPES pH7.4) and dejellied in 2% L-cysteine/1×MMR pH8.2. To generate Xenopus laevis transgenic for p24α3-GFP, a linear 2274-base pair SalI/NarI DNA fragment, containing a 529-base pair Xenopus POMC gene A promoter fragment (pPOMC ) and a cDNA encoding the Xenopus p24α3 protein (construct pPOMC-p24α3-GFP) with the enhanced green fluorescent protein (GFP) protein fused in frame to its C-terminus (p24α3-GFP fusion protein), was used for stable Xenopus transgenesis , . The linearized transgene DNA fragment was mixed with sperm nuclei prepared as described previously , , incubated at room temperature (RT) for 15 min and injected into the unfertilized eggs. Normally cleaving embryos were selected at the 4-cell stage and grown in 0.1×MMR/6% Ficoll-400 (Sigma) with 50 µg/ml gentamycin (Gibco/BRL) at 18°C until gastrulation (stage 12 ) was reached and then in 0.1×MMR containing gentamycin at RT. In living stage-45 embryos  anaesthetized with 0.25 mg/ml MS222 (3-aminobenzoic acid ethyl ester; Sigma) the presence of GFP fluorescence was examined using a Leica MZ FLIII fluorescence stereomicroscope and photographs were taken with a Leica DC200 color camera using the Leica DCviewer software. A number of injection rounds resulted in a number of F0 animals expressing the fusion protein at various levels. F1 transgenic Xenopus laevis offspring were generated by in vitro fertilization (IVF), either using pieces of testes from male transgenic frogs and eggs harvested from wild-type females or using pieces of testes from male wild-types and eggs from transgenic females. For IVF, the testes of male Xenopus frogs were isolated and incubated for 10 min with a monolayer of eggs harvested from females frogs injected with Pregnyl 18 hr before. 0.1×MMR was added and properly dividing embryos were selected and screened for GFP fluorescence as described above for F0 tadpoles. To generate p24δ2-transgenic Xenopus, a linear 2166-base pair SalI/NarI DNA fragment (construct pPOMC-p24δ2-GFP), containing the Xenopus POMC gene A promoter (pPOMC ) and a cDNA encoding the Xenopus p24δ2 protein with the enhanced GFP protein fused in frame to its C-terminus (p24δ2-GFP fusion protein), was used analogous to the method described above to generate the animals transgenic for p24α3-GFP. A number of injection rounds resulted in a number of F0 animals transgenic for p24δ2-GFP and expressing the fusion protein at various levels. F1 transgenic Xenopus laevis offspring were generated by IVF, using pieces of testes from male transgenic frogs and eggs harvested from wild-type females.
Western blot analysis
Western blot analysis was performed as described previously . Proteins on the blot were visualized by enhanced chemiluminescence using the Lumi-lightPLUS Western blotting substrate (Roche), detected in an Epi Chemi II Darkroom equipped with a 12-bit SensiCam CCD camera and quantified using the Labworks 4.0 program (UVP BioImaging systems, Cambridge, United Kingdom).
Freshly dissected neurointermediate lobes (NILs) were homogenized on ice in cold 0.1% tri-fluoroacetic acid (TFA; mass spectrometry grade; Merck, Darmstadt, Germany) and kept on ice for 15 min. Subsequently, cell debris was pelleted by centrifugation (10 min, >15000×g, 4°C), and supernatants were collected and diluted in 0.1% TFA. Of the diluted homogenates, 1 µl was mixed with 10 µl of a saturated solution of α-cyano-4-hydroxy-cinnamic acid (CHCA; Sigma) in a 1∶1 (v/v) acetonitrile (Sigma)/0.1% TFA mixture. A volume of 0.5 µl of this mixture was spotted on the sample plate and samples were measured in reflectron mode on a Bruker Biflex III machine (Bruker, FRG). The machine was calibrated with the reference peptides Bradykinin fragment 1–7 (757.3997Da; Sigma), Angiotensin II (1,046.5423Da; Sigma), synthetic peptide P14R (1,533.8582Da; Sigma) and human ACTH fragment 18–39 (2,465.1989Da; Sigma).
Pulse and pulse-chase analysis
For metabolic cell labeling, NILs from wild-type and transgenic Xenopus were dissected and preincubated in Ringer's medium (112 mM NaCl, 2mM KCl, 2 mM CaCl2, 15 mM HEPES pH7.4, 0.3 mg/ml BSA and 2 mg/ml glucose) at RT for 15 min, pulse labeled in Ringer's medium with 5 mCi/ml Tran35S-label (ICN Radiochemicals), chased in Ringer's medium supplemented with 0.5 mM L-methionine for the indicated time periods and homogenized as described previously . For the double-labeling experiments to examine the sulfation of newly synthesized POMC, the NILs were pulse labeled for 15 min with 6.67 mCi/ml Na2[35S]SO4 (ICN Radiochemicals) and 1mCi/ml L-[4,5-3H]Lysine monohydrochloride (Amersham Biosciences), rinsed briefly to remove free label and homogenized. Parts of the lysates and chase media were analyzed directly on SDS-PAGE, while the remainder was used for Western blot or HPLC analyses. For quantification, the radioactively double-labeled NIL proteins were separated by SDS-PAGE, and slices (2 mm×1 cm) were cut from the gel, incubated overnight with 1 ml 35% H2O2 (Acros Organics) at 70°C, 4 ml of OptiPhase ‘HiSafe’ 3 counting liquid (Perkin Elmer Life Sciences) was added, 3H- and 35S-dpm were counted on a Wallac 1410 Liquid Scintillation Counter (Pharmacia) and the amount of incorporated [35S]SO4 relative to the amount of incorporated 3H-lysine was determined for the newly synthesized POMC.
N-linked glycogroups were removed from the newly synthesized NIL proteins by boiling the homogenates in 6 mM HEPES/0.06% SDS pH7.4 for 10 min, cooling to room temperature, adding 1 µl 12.5% NP40/2.5 mM phenylmethylsulphonyl fluoride (PMSF)/0.25 mg/ml trypsin inhibitor and 1 U Peptidyl N-glycosidase F (PNGaseF; Roche) and incubating the samples overnight at 37°C. The deglycosylated proteins were resolved by 20% SDS-PAGE and visualized by autoradiography.
For the separation of the newly synthesized POMC-derived peptides, radiolabelled NIL lysates were subjected to HPLC analysis as described previously .
Transmission electron microscopy
For ultrastructural studies, NILs were freshly isolated from wild-type and transgenic Xenopus and fixed overnight at 4°C in 2% glutaraldehyde in 0.1 M phosphate buffer (PB, pH7.3). After rinsing in the same buffer, fixed tissues were osmicated for one hour in 1% osmium tetroxide in 0.1 M PB, rinsed in PB, dehydrated through graded series of alcohol and embedded in Epon 812. One-micron thick sections were cut, stained with toluidine blue and examined in a phase-contrast microscope (Dialux 20, Leitz). Ultrathin sections were cut, double contrasted with uranyl-acetate/lead-citrate and photographed using a transmission electron microscope (JEOL1010).
For freeze substitution and low-temperature embedding, the tissue was rapidly frozen by a Leica EM High-Pressure Freezing system (HPF, Leica Microsystems) and transferred to the precooled chamber (−90°C) of a CS auto freeze substitution apparatus (Reichert-Jung, Germany). Freeze substitution was performed according to standard procedures. The tissue was immersed for 72 hours in acetone containing 0.5% uranyl acetate as fixing agent at −90°C and the temperature was then raised stepwise 4°C per hour to −45°C. Prior to infiltration with Lowicryl HM20 resin (Bio-Rad Richmond, California; USA), the tissue was washed several times with acetone at −45°C to remove water and excess uranyl acetate. The embedding process was carried out at −45°C in three stages, with a progressively increasing ratio of resin to acetone. Diffuse UV-radiation (360nm) was used to catalyze polymerization first at −45°C overnight and then at room temperature for 24 hrs. Thin sections were cut on a Reichert Ultracut-E and mounted on one-hole nickel grids coated with a formvar film.
For postembedding immunohistochemistry, ultrathin Lowicryl sections were washed for 10 min in phosphate buffered saline (PBS, pH 7.4) containing 0.1% sodium borohydride and 50 mM glycine, and for 10 min in PBS containing 0.5% BSA and 0.1% cold fish skin gelatine (PBG). For immunolabeling, sections were incubated overnight at 4°C in drops of PBG containing anti-GFP or anti-POMC antibody. Sections were washed for 20 min in PBG, incubated with protein A-labeled 10 nm gold markers, washed in PBS and postfixed with 2.5% glutaraldehyde in PB for 5 min to minimize loss of gold label during the contrasting steps. After washing with distilled water, sections were contrasted in uranyl acetate and studied using a JEOL1010 TEM electron microscope.
Quantification and statistics
Statistical evaluation was performed using unpaired two-tailed t-tests. In those cases where the variances were significantly different, Welch's correction was used. Calculations were performed using the GraphPad Prism 4 program (GraphPad Software).
We would like to thank Ron Engels for animal care, Folkert Verkaar and Huub op den Camp for help with MALDI-TOF MS analyses, Rick van Huizen for help with the generation of transgenic frogs and Paul Jap for expert help with electron microscopy analyses. We also thank Drs Jack Fransen, Shige Tanaka, Wim Van de Ven and Bé Wieringa for providing antibodies.
Conceived and designed the experiments: GM JS GB. Performed the experiments: TH JS GB. Analyzed the data: TH GM JS GB. Contributed reagents/materials/analysis tools: GB. Wrote the paper: GM JS.
- 1. Palade G (1975) Intracellular aspects of the process of protein synthesis. Science 189: 347–358.
- 2. Carney GE, Bowen NJ (2004) p24 proteins, intracellular trafficking, and behavior: Drosophila melanogaster provides insights and opportunities. Biol Cell 96: 271–278.
- 3. Emery G, Gruenberg J, Rojo M (1999) The p24 family of transmembrane proteins at the interface between endoplasmic reticulum and Golgi apparatus. Protoplasma V207: 24–30.
- 4. Dominguez M, Dejgaard K, Füllekrug J, Dahan S, Fazel A, et al. (1998) gp25L/emp24/p24 protein family members of the cis-Golgi network bind both COP I and II coatomer. J Cell Biol 140: 751–765.
- 5. Stamnes MA, Craighead MW, Hoe MH, Lampen N, Geromanos S, et al. (1995) An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding. Proc Natl Acad Sci U S A 92: 8011–8015.
- 6. Schimmöller F, Singer-Krüger B, Schröder S, Krüger U, Barlowe C, et al. (1995) The absence of Emp24p, a component of ER-derived COPII-coated vesicles, causes a defect in transport of selected proteins to the Golgi. EMBO J 14: 1329–1339.
- 7. Belden WJ, Barlowe C (1996) Erv25p, a component of COPII-coated vesicles, forms a complex with Emp24p that is required for efficient endoplasmic reticulum to Golgi transport. J Biol Chem 271: 26939–26946.
- 8. Marzioch M, Henthorn DC, Herrmann JM, Wilson R, Thomas DY, et al. (1999) Erp1p and Erp2p, partners for Emp24p and Erv25p in a yeast p24 complex. Mol Biol Cell 10: 1923–1938.
- 9. Muñiz M, Nuoffer C, Hauri HP, Riezman H (2000) The Emp24 complex recruits a specific cargo molecule into endoplasmic reticulum-derived vesicles. J Cell Biol 148: 925–930.
- 10. Rojo M, Pepperkok R, Emery G, Kellner R, Stang E, et al. (1997) Involvement of the transmembrane protein p23 in biosynthetic protein transport. J Cell Biol 139: 1119–1135.
- 11. Gommel D, Orci L, Emig EM, Hannah MJ, Ravazzola M, et al. (1999) p24 and p23, the major transmembrane proteins of COPI-coated transport vesicles, form hetero-oligomeric complexes and cycle between the organelles of the early secretory pathway. FEBS Lett 447: 179–185.
- 12. Lavoie C, Paiement J, Dominguez M, Roy L, Dahan S, et al. (1999) Roles for alpha(2)p24 and COPI in endoplasmic reticulum cargo exit site formation. J Cell Biol 146: 285–299.
- 13. Rojo M, Emery G, Marjomaki V, McDowall AW, Parton RG, et al. (2000) The transmembrane protein p23 contributes to the organization of the Golgi apparatus. J Cell Sci 113 (Pt 6): 1043–1057.
- 14. Emery G, Parton RG, Rojo M, Gruenberg J (2003) The trans-membrane protein p25 forms highly specialized domains that regulate membrane composition and dynamics. J Cell Sci 116: 4821–4832.
- 15. Springer S, Chen E, Duden R, Marzioch M, Rowley A, et al. (2000) The p24 proteins are not essential for vesicular transport in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 97: 4034–4039.
- 16. Denzel A, Otto F, Girod A, Pepperkok R, Watson R, et al. (2000) The p24 family member p23 is required for early embryonic development. Curr Biol 10: 55–58.
- 17. Kuiper RP, Martens GJ (2000) Prohormone transport through the secretory pathway of neuroendocrine cells. Biochem Cell Biol 78: 289–298.
- 18. Holthuis JC, Jansen EJ, van Riel MC, Martens GJ (1995) Molecular probing of the secretory pathway in peptide hormone-producing cells. J Cell Sci 108 (Pt 10): 3295–3305.
- 19. Rötter J, Kuiper RP, Bouw G, Martens GJ (2002) Cell-type-specific and selectively induced expression of members of the p24 family of putative cargo receptors. J Cell Sci 115: 1049–1058.
- 20. Jansen EJR, Holling TM, van Herp F, Martens GJM (2002) Transgene-driven protein expression specific to the intermediate pituitary melanotrope cells of Xenopus laevis. FEBS Letters 516: 201–207.
- 21. Bouw G, Van Huizen R, Jansen EJ, Martens GJ (2004) A cell-specific transgenic approach in Xenopus reveals the importance of a functional p24 system for a secretory cell. Mol Biol Cell 15: 1244–1253.
- 22. Chen F, Hasegawa H, Schmitt-Ulms G, Kawarai T, Bohm C, et al. (2006) TMP21 is a presenilin complex component that modulates γ-secretase but not ε-secretase activity. 440: 1208–1212.
- 23. Vetrivel KS, Gong P, Bowen JW, Cheng H, Chen Y, et al. (2007) Dual roles of the transmembrane protein p23/TMP21 in the modulation of amyloid precursor protein metabolism. Mol Neurodegener 2: 4.
- 24. Collin RW, van den Hurk WH, Martens GJ (2005) Biosynthesis and differential processing of two pools of amyloid-beta precursor protein in a physiologically inducible neuroendocrine cell. J Neurochem 94: 1015–1024.
- 25. Martens GJ (1986) Expression of two proopiomelanocortin genes in the pituitary gland of Xenopus laevis: complete structures of the two preprohormones. Nucleic Acids Res 14: 3791–3798.
- 26. van Kuppeveld FJ, van Horssen AM, Martens GJ (1997) Intracellular transport, sorting, and proteolytic processing of regulated secretory proteins does not require protein sulfation. Mol Cell Endocrinol 136: 29–35.
- 27. Monigatti F, Gasteiger E, Bairoch A, Jung E (2002) The Sulfinator: predicting tyrosine sulfation sites in protein sequences. Bioinformatics 18: 769–770.
- 28. Simpson JC, Nilsson T, Pepperkok R (2006) Biogenesis of tubular ER-to-Golgi transport intermediates. Mol Biol Cell 17: 723–737.
- 29. Elrod-Erickson MJ, Kaiser CA (1996) Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations. Mol Biol Cell 7: 1043–1058.
- 30. Belden WJ, Barlowe C (2001) Deletion of yeast p24 genes activates the unfolded protein response. Mol Biol Cell 12: 957–969.
- 31. Majoul I, Sohn K, Wieland FT, Pepperkok R, Pizza M, et al. (1998) KDEL receptor (Erd2p)-mediated retrograde transport of the cholera toxin A subunit from the Golgi involves COPI, p23, and the COOH terminus of Erd2p. J Cell Biol 143: 601–612.
- 32. Majoul I, Straub M, Hell SW, Duden R, Soling HD (2001) KDEL-cargo regulates interactions between proteins involved in COPI vesicle traffic: measurements in living cells using FRET. Dev Cell 1: 139–153.
- 33. Gupta V, Swarup G (2006) Evidence for a role of transmembrane protein p25 in localization of protein tyrosine phosphatase TC48 to the ER. J Cell Sci 119: 1703–1714.
- 34. Wen C, Greenwald I (1999) p24 proteins and quality control of LIN-12 and GLP-1 trafficking in Caenorhabditis elegans. J Cell Biol 145: 1165–1175.
- 35. Stark C, Breitkreutz B-J, Reguly T, Boucher L, Breitkreutz A, et al. (2006) BioGRID: a general repository for interaction datasets. Nucl Acids Res 34: D535–539.
- 36. Mironov AA, Mironov AAJ, Beznoussenko GV, Trucco A, Lupetti P, et al. (2003) ER-to-Golgi carriers arise through direct en bloc protrusion and multistage maturation of specialized ER exit domains. Dev Cell 5: 583–594.
- 37. Kuiper RP, Bouw G, Janssen KP, Rötter J, van Herp F, et al. (2001) Localization of p24 putative cargo receptors in the early secretory pathway depends on the biosynthetic activity of the cell. Biochem J 360: 421–429.
- 38. Chu DT, Klymkowsky MW (1989) The appearance of acetylated alpha-tubulin during early development and cellular differentiation in Xenopus. Dev Biol 136: 104–117.
- 39. Kroll K, Amaya E (1996) Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. Development 122: 3173–3183.
- 40. Sparrow DB, Latinkic B, Mohun TJ (2000) A simplified method of generating transgenic Xenopus. Nucleic Acids Res 28: E12.
- 41. Nieuwkoop PD, Faber J (1967) Normal table of Xenopus laevis (Daudin): a systematical and chronological survey of the development from the fertilized egg till the end of metamorphosis;. In: Nieuwkoop PD, Faber J, editors. Amsterdam: North-Holland Publishing Company.
- 42. Kuiper RP, Waterham HR, Rötter J, Bouw G, Martens GJ (2000) Differential induction of two p24delta putative cargo receptors upon activation of a prohormone-producing cell. Mol Biol Cell 11: 131–140.
- 43. Braks JA, Martens GJ (1994) 7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway. Cell 78: 263–273.
- 44. Martens GJ, Biermans PP, Jenks BG, Van Overbeeke AP (1982) Biosynthesis of two structurally different pro-opiomelanocortins in the pars intermedia of the amphibian pituitary gland. Eur J Biochem 126: 17–22.