Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Cis-Cotranscription of Two Beta Globin Genes during Chicken Primitive Hematopoiesis

  • Hiroki Nagai,

    Affiliation Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

  • Guojun Sheng

    To whom correspondence should be addressed. E-mail: sheng@cdb.riken.jp

    Affiliation Laboratory for Early Embryogenesis, RIKEN Center for Developmental Biology, Kobe, Hyogo, Japan

Cis-Cotranscription of Two Beta Globin Genes during Chicken Primitive Hematopoiesis

  • Hiroki Nagai, 
  • Guojun Sheng
PLOS
x

Abstract

Chicken beta globin locus contains four genes, two of which, rho and epsilon, are expressed from the earliest stage of primitive hematopoiesis. Here we show that the transcription of these two genes in the nucleus engages in “on/off” phases. During each “on” phase, cotranscription of rho and epsilon in cis is favored. We propose that these two chicken beta globin genes are transcribed not by competing for a transcription initiation complex, but in a cooperative way.

Introduction

Studies of beta globin locus have been instrumental in understanding fundamental mechanisms of transcriptional regulation [1], [2]. One unresolved question is whether transcription of two or more beta globin genes can occur simultaneously within a single locus. Chicken beta globin locus, located near the distal tip of chromosome 1, contains four genes (rho, betaH, betaA and epsilon) (Fig. 1A) [3]. Chicken embryo establishes its circulation at around stage HH13, and primitive blood differentiation is initiated at HH7 with the expression of rho and epsilon genes [4]. By HH10, all blood cells are positive for both transcripts in their cytoplasm (Fig. 1B,C).

thumbnail
Figure 1. Primary transcript staining of rho and epsilon genes.

A) Schematic diagram of chicken beta globin locus. B,C) rho (B) and epsilon (C) cytoplasmic probes stain in all blood cells at stage HH10. D,E) Intron probe against rho (D) or epsilon (E) reveals three types of blood cell staining: negative, single positive and double positive. Left: Sections of HH10 embryo; right: Sections of HH13 embryo.

https://doi.org/10.1371/journal.pone.0000703.g001

Results

To assess the dynamics of primary transcription taking place in the nucleus, we generated probes against intron 2 of rho and epsilon, and performed whole mount in situ with pre-circulation stage embryos. With either rho or epsilon specific intron probe, we detected three types of primitive blood cells, with their nucleus having 0, 1 or 2 positive signals (Fig. 1D,E). We interpreted them as representing nuclei with both loci off (−/−), one locus on (+/−) and both loci on (+/+), respectively, for a particular globin gene.

A significant percentage of −/− nuclei was seen with either rho or epsilon, suggesting that chicken beta locus transcription engages in on/off cycles as described in primitive blood cells in mouse. We therefore counted −/−, +/− and +/+ nuclei to determine the relative percentage of each state for stage HH10 embryos. We detected 34.1% of nuclei to be −/−, 50.2% +/− and 15.7% +/+ for rho globin (n = 801 nuclei); and 11.7% −/−, 39.0% +/− and 49.2% +/+ for epsilon globin (n = 894 nuclei) (Table 1). This indicates that for each locus there is approximately 40% probability to transcribe rho and 70% to transcribe epsilon.

thumbnail
Table 1. Percentages of nuclei with −/−, +/− or +/+ staining for rho and epsilon single intron staining at HH10.

https://doi.org/10.1371/journal.pone.0000703.t001

From these ratios we inferred that both beta globin loci in a given nucleus are making similar stochastic choice to be either on or off, because otherwise we would expect skewed ratios among these three states. This led to an obvious question regarding the relationship between rho and epsilon transcription within a single locus. We used double fluorescent in situ to try to discern three possible scenarios: mutually exclusive, cooperative and random. Majority of cells were either rho(+/+)epsilon(+/+), rho(+/−)epsilon(+/+) or rho(+/+)epsilon(+/−) (Figs. 2A-C; 3), for embryos of all stages (HH10-13) examined. In total, these three co-expression combinations represented approximately 70% of all nuclei scored (n = 1833). This would rule out the “mutually exclusive” scenario because it predicts an under-representation of individual loci double positive for both transcripts. A similar observation in mouse, however, was interpreted as a consequence of primary transcripts' half life being comparable to on/off cycle time [5][7]. We therefore took advantage of relatively high percentages of rho(+/−) and epsilon(+/−) nuclei seen in single probe in situ. We reasoned that if we focus on rho(+/−)epsilon(+/−) nuclei, regardless of primary transcripts' half life, a mutually exclusive scenario would predict +−/−+>>++/−−; a cooperative scenario ++/−−>>+−/−+; and a random scenario ++/−− = +−/−+. We found that over 99% of rho(+/−)epsilon(+/−) nuclei were ++/−− (750/753 nuclei) (Fig. 2D). This indicates that not only cotranscription in cis of rho and epsilon can occur, but cooperative cotranscription is preferred. We scored percentages of different rho and epsilon expression combinations using individual nucleus as a unit, because this allowed us to distinguish ++/−− from +−/−+ scenario. A re-analysis of our data using individual locus as a unit yielded a similar conclusion. Among all loci with at least one signal for either rho or epsilon (n = 3446), we found 2872 (83.3%) to be rho+epsilon+, 402 (11.7%) rhoepsilon+ and 172 (5.0%) rho+epsilon.

thumbnail
Figure 2. Double staining with rho (green) and epsilon (red) probes reveal significant colocalization (merge). A: ++/++; B: ++/+−; C: ++/−+; D: ++/−−.

https://doi.org/10.1371/journal.pone.0000703.g002

thumbnail
Figure 3. Schematic diagram of 10 possible combinations for rho and epsilon expression and their relative percentages.

rho(−/−)epsilon(−/−) is not counted. rho(+/−)epsilon(+/−) data includes both scenarios. The statistics on ++/−− and +−/−+ in the text includes data from this and additional analysis focusing only on rho(+/−)epsilon(+/−) nuclei.

https://doi.org/10.1371/journal.pone.0000703.g003

Our nucleus-based fluorescent in situ signal counting, however, yielded higher ratio between +/+ and +/− nuclei for both rho and epsilon (1.32 and 1.87, respectively) than observed with stage HH10 embryos (0.31 and 1.26, respectively). Since for this analysis we used primarily older stage pre-circulation embryos when individual blood cells are more readily distinguishable, the difference suggested that the probability for each locus to transcribe either rho or epsilon increases as blood cells mature. Because the increase for rho(+/+)/rho(+/−) was relatively large, we examined rho single probe stained blood cells in sections with stage HH11 embryos. We observed 23.6% (220/934) +/+, 53.4% (499/934) +/− and 23.0% (215/934) −/− nuclei, indicating an increase of Pon/locus for rho from 40% at HH10 to 49% at HH11. This rapid increase suggests that immediately after the establishment of circulation after HH13, A vast majority of loci will be transcribing both rho and epsilon, two predominant beta globins in primitive blood cells.

Discussion

In summary, our data argue against the mutually exclusive model proposed for mouse primitive erythrocytes [5][7]. The only way to explain our data with this model, also called flip-flop or alternating model, is to modify it by adding a two step on/off component. In this “two-step” model, the first on/off decision would be permissive. An off state would have no transcription, while an on state would allow transcription to take place. The second on/off decision would follow a rapid alternating initiation, possible by competing for LCR, of either rho or epsilon. If this is the case, however, a reinterpretation of mouse data would undermine the basis for the model in the first place. A simpler interpretation of our data is that, at least in chicken, rho and epsilon are not competing for a single transcription initiation promoting complex. Successful initiation of one gene rather greatly increases the probability of the other to be transcribed.

Materials and Methods

Fertile chicken eggs were purchased from Shiroyama Farm (Kanagawa, Japan) and incubated at 38.5 degrees to desired stages. Intron probes for rho and epsilon correspond to exact intron 2 sequences (NCBI #L17432). In situ hybridization followed previous described protocol [4] with the only modification that embryos were fixed following incubation within 2 minutes. Single color staining used alkaline phosphatase coupled antibodies, and two color fluorescent staining used probes separately labeled with fluorescein or digoxygenin, revealed stepwise with HRP coupled antibodies using tyramide signal amplification system (PerkinElmer, #NEL753). Counting was performed in 16 um sections for single staining, and in whole mount with isolated blood cells for double staining.

Author Contributions

Conceived and designed the experiments: GS. Performed the experiments: HN. Analyzed the data: GS HN. Wrote the paper: GS.

References

  1. 1. Stamatoyannopoulos G (2005) Control of globin gene expression during development and erythroid differentiation. Exp Hematol 33(3): 259–71.G. Stamatoyannopoulos2005Control of globin gene expression during development and erythroid differentiation.Exp Hematol33325971
  2. 2. Evans T, Felsenfeld G, Reitman M (1990) Control of globin gene transcription. Annu Rev Cell Biol 6: 95–124.T. EvansG. FelsenfeldM. Reitman1990Control of globin gene transcription.Annu Rev Cell Biol695124
  3. 3. Dolan M, Sugarman BJ, Dodgson JB, Engel JD (1981) Chromosomal arrangement of the chicken beta-type globin genes. Cell 24(3): 669–77.M. DolanBJ SugarmanJB DodgsonJD Engel1981Chromosomal arrangement of the chicken beta-type globin genes.Cell24366977
  4. 4. Nakazawa F, Nagai H, Shin M, Sheng G (2006) Negative regulation of primitive hematopoiesis by the FGF signaling pathway. Blood 108(10): 3335–43.F. NakazawaH. NagaiM. ShinG. Sheng2006Negative regulation of primitive hematopoiesis by the FGF signaling pathway.Blood10810333543
  5. 5. Trimborn T, Gribnau J, Grosveld F, Fraser P (1999) Mechanisms of developmental control of transcription in the murine alpha- and beta-globin loci. Genes Dev 13(1): 112–24.T. TrimbornJ. GribnauF. GrosveldP. Fraser1999Mechanisms of developmental control of transcription in the murine alpha- and beta-globin loci.Genes Dev13111224
  6. 6. Wijgerde M, Grosveld F, Fraser P (1995) Transcription complex stability and chromatin dynamics in vivo. Nature 377(6546): 209–13.M. WijgerdeF. GrosveldP. Fraser1995Transcription complex stability and chromatin dynamics in vivo.Nature377654620913
  7. 7. Gribnau J, de Boer E, Trimborn T, Wijgerde M, Milot E, et al. (1998) Chromatin interaction mechanism of transcriptional control in vivo. Embo J 17(20): 6020–7.J. GribnauE. de BoerT. TrimbornM. WijgerdeE. Milot1998Chromatin interaction mechanism of transcriptional control in vivo.Embo J172060207