A: Western blotting results for endogenous YAP expression in 11 ovarian cancer cell lines. B: Diagram of the main functional structural domains and phosphorylation sites in human YAP (full length and C-terminal deleted, YAP-ΔC, forms). C: Western blotting results for the expression of FLAG-tagged YAP-5SA and YAP-5SA-ΔC in established stable cell lines. D: Growth curves for cells that stably expressed YAP-5SA (upper panel) and YAP-5SA-ΔC (lower panel) and their control cells during a 7-day culture period. E: Images and quantitative results for flat plate colony formation assays. Cells that expressed YAP-5SA or YAP-5SA-ΔC and their control cells were cultured in 6-well plates for 2 weeks. F: Images and quantitative results for colonies grown in soft agar. Cells that expressed YAP-5SA or YAP-5SA-ΔC and their control cells were seeded in soft agar for 3 weeks. G: Images and quantitative results for xenografts grown in nude mice. Mice were subcutaneously injected with YAP-5SA expressing and control cells. Each point is the mean of 3 experiments. Error bars represent s.d. 's; n = 5. Statistically significant differences as compared with a control as determined by Student's t-test are denoted by *(P<0.05) or **(P<0.01).
A: Viability of YAP-5SA and YAP-5SA-ΔC expressing cells after treatment with Taxol or CDDP, as assessed by MTT assay. B: Western blotting results for the apoptosis markers cleaved caspase 3 and PARP in YAP-5SA expressing and control cells after treatment with the indicated doses of CDDP for 48 h. C-D: Flow cytometry results for apoptosis of A2780CP cells with or without YAP-5SA-DC transfection and after treatment with different doses of CDDP for 48 h. E: Western blotting results for cleaved caspase 3 and PARP in YAP-5SA-DC expressing and control cells after treatment with the indicated doses of CDDP for 48 h. F-G: Images and quantitative results for in vivo tumorigenic capacity of A2780CP cells with or without YAP-5SA-ΔC expression. Nude mice were injected with CDDP through a caudal vein once each week for four weeks after tumor xenografts reached 5 mm in diameter. H: IHC results for the proliferation marker Ki67 on the indicated tumor tissue sections. Scale bar = 100 μM.
Citation: Xia Y, Chang T, Wang Y, Liu Y, Li W, Li M, et al. (2016) Correction: YAP Promotes Ovarian Cancer Cell Tumorigenesis and Is Indicative of a Poor Prognosis for Ovarian Cancer Patients. PLoS ONE 11(3): e0152712. doi:10.1371/journal.pone.0152712
Published: March 25, 2016
Copyright: © 2016 Xia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.