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Open Access
Peer-reviewed
Research Article
Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?
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Mariana Andrade Torres ,
Contributed equally to this work with: Mariana Andrade Torres, André Furugen Cesar de Andrade
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Rommy Díaz ,
¶‡ These authors also contributed equally to this work.
Affiliation Center of Excellence in Biotechnology of Reproduction, University of La Frontera, Temuco, Araucania, Chile
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Rodrigo Boguen ,
¶‡ These authors also contributed equally to this work.
Affiliation Center of Excellence in Biotechnology of Reproduction, University of La Frontera, Temuco, Araucania, Chile
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Simone Maria Massami Kitamura Martins ,
¶‡ These authors also contributed equally to this work.
Affiliations Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil, Laboratory of Swine Research, Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Gisele Mouro Ravagnani ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Diego Feitosa Leal ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Melissa de Lima Oliveira ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Bruno Bracco Donatelli Muro ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Beatriz Martins Parra ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Flávio Vieira Meirelles ,
¶‡ These authors also contributed equally to this work.
Affiliation Department of Veterinary Medicine, School of Animal Sciences and Food Engineerig, University of São Paulo, Pirassununga, São Paulo, Brazil
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Frederico Ozanan Papa ,
¶‡ These authors also contributed equally to this work.
Affiliation Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
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José Antônio Dell’Aqua Jr ,
¶‡ These authors also contributed equally to this work.
Affiliation Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
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Marco Antônio Alvarenga ,
¶‡ These authors also contributed equally to this work.
Affiliation Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil
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Aníbal de Sant’Anna Moretti ,
¶‡ These authors also contributed equally to this work.
Affiliation Laboratory of Swine Research, Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Néstor Sepúlveda ,
¶‡ These authors also contributed equally to this work.
Affiliation Center of Excellence in Biotechnology of Reproduction, University of La Frontera, Temuco, Araucania, Chile
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André Furugen Cesar de Andrade
Contributed equally to this work with: Mariana Andrade Torres, André Furugen Cesar de Andrade
* E-mail: andrefc@usp.br
Affiliation Laboratory of Andrology and Technology of Swine Embryos, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of São Paulo, Pirassununga, São Paulo, Brazil
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Novel Flow Cytometry Analyses of Boar Sperm Viability: Can the Addition of Whole Sperm-Rich Fraction Seminal Plasma to Frozen-Thawed Boar Sperm Affect It?
- Mariana Andrade Torres,
- Rommy Díaz,
- Rodrigo Boguen,
- Simone Maria Massami Kitamura Martins,
- Gisele Mouro Ravagnani,
- Diego Feitosa Leal,
- Melissa de Lima Oliveira,
- Bruno Bracco Donatelli Muro,
- Beatriz Martins Parra,
- Flávio Vieira Meirelles
- Published: August 16, 2016
- https://doi.org/10.1371/journal.pone.0160988