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Open Access
Peer-reviewed
Research Article
Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering
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Sisi Lai ,
Contributed equally to this work with: Sisi Lai, Shu Wei
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Shu Wei ,
Contributed equally to this work with: Sisi Lai, Shu Wei
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Bentian Zhao,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Zhen Ouyang,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Quanjun Zhang,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Nana Fan,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Zhaoming Liu,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Yu Zhao,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Quanmei Yan,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Xiaoqing Zhou,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Li Li,
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Jige Xin,
Affiliation Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, Animal Science and Technology College, Yunnan Agricultural University, Kunming, China
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Yangzhi Zeng ,
* E-mail: zou_qingjian@gibh.ac.cn (Q. Zou); lai_liangxue@gibh.ac.cn (L. Lai); bnwxzjjx@yahoo.com.cn (Y. Zeng)
Affiliation Key Laboratory of Banna Mini-pig Inbred Line of Yunnan Province, Animal Science and Technology College, Yunnan Agricultural University, Kunming, China
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Liangxue Lai ,
* E-mail: zou_qingjian@gibh.ac.cn (Q. Zou); lai_liangxue@gibh.ac.cn (L. Lai); bnwxzjjx@yahoo.com.cn (Y. Zeng)
Affiliations CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China, Jilin Provincial Key Laboratory of Animal Embryo Engineering, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China
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Qingjian Zou
* E-mail: zou_qingjian@gibh.ac.cn (Q. Zou); lai_liangxue@gibh.ac.cn (L. Lai); bnwxzjjx@yahoo.com.cn (Y. Zeng)
Affiliation CAS Key Laboratory of Regenerative Biology, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
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Generation of Knock-In Pigs Carrying Oct4-tdTomato Reporter through CRISPR/Cas9-Mediated Genome Engineering
- Sisi Lai,
- Shu Wei,
- Bentian Zhao,
- Zhen Ouyang,
- Quanjun Zhang,
- Nana Fan,
- Zhaoming Liu,
- Yu Zhao,
- Quanmei Yan,
- Xiaoqing Zhou
- Published: January 12, 2016
- https://doi.org/10.1371/journal.pone.0146562