%PDF-1.6
%
1 0 obj
<>
endobj
2 0 obj
<>stream
Fernanda B. Fusco, Diego J. Gomes, Kely C. S. Bispo, Veronica P. Toledo, Denise F. Barbeiro, Vera L. Capelozzi, Luzia N. S. Furukawa, Ana P. P. Velosa, Walcy R. Teodoro, Joel C. Heimann, Eder C. R. Quintao, Marisa Passarelli, Edna R. Nakandakare, Sergio Catanozi
Low-sodium diet induces atherogenesis regardless of lowering blood pressure in hypertensive hyperlipidemic mice
www.plosone.org
www.plosone.org
endstream
endobj
3 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 12 0 R/Contents 13 0 R/TrimBox[0 0 612 792]>>
endobj
12 0 obj
[14 0 R 15 0 R 16 0 R 17 0 R 18 0 R 19 0 R 20 0 R 21 0 R]
endobj
14 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref001)>>
endobj
15 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref002)>>
endobj
16 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref003)>>
endobj
17 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref004)>>
endobj
18 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref005)>>
endobj
19 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref006)>>
endobj
20 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref007)>>
endobj
21 0 obj
<>/Border[0 0 0]/A 22 0 R>>
endobj
22 0 obj
<>
endobj
13 0 obj
[23 0 R 24 0 R 25 0 R 26 0 R 27 0 R 28 0 R 29 0 R 30 0 R 31 0 R 32 0 R 33 0 R 34 0 R]
endobj
23 0 obj
<>stream
q
0.83 0.64 0.02 0 k
466.1291 674.9858 m
470.8913 674.9858 l
h
f*
475.3701 674.9858 m
480.1323 674.9858 l
h
f*
401.4992 635.9811 m
406.2614 635.9811 l
h
f*
242.3622 610.0157 m
247.1244 610.0157 l
h
f*
271.1055 596.9764 m
275.8677 596.9764 l
h
f*
347.5276 571.011 m
352.2898 571.011 l
h
f*
446.0598 544.989 m
450.822 544.989 l
h
f*
0 g
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 706.0535 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Introduction)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 689.1023 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Observational)Tj
5.8109 0 Td
(studies)Tj
2.9821 0 Td
(in)Tj
1.0261 0 Td
(human)Tj
3.0727 0 Td
(populations)Tj
4.9436 0 Td
(have)Tj
2.0523 0 Td
(shown)Tj
2.846 0 Td
(conflicting)Tj
4.4957 0 Td
(results)Tj
2.8006 0 Td
(regarding)Tj
4.0875 0 Td
(the)Tj
1.4627 0 Td
(asso-)Tj
-35.5802 -1.3039 Td
(ciation)Tj
2.965 0 Td
(of)Tj
1.0148 0 Td
(sodium)Tj
3.2314 0 Td
(intake)Tj
2.6873 0 Td
(with)Tj
1.9955 0 Td
(cardiovascular)Tj
6.0038 0 Td
(events)Tj
2.7269 0 Td
(and)Tj
1.7348 0 Td
(mortality)Tj
3.9118 0 Td
([)Tj
0.83 0.64 0.02 0 k
(1)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2642 0 Td
(2)Tj
0 g
(].)Tj
1.2586 0 Td
(Low-sodium)Tj
5.3121 0 Td
(\(LS\))Tj
-34.1062 -1.2982 Td
(diets)Tj
2.0976 0 Td
(adversely)Tj
3.9174 0 Td
(influence)Tj
3.9175 0 Td
(markers)Tj
3.4922 0 Td
(for)Tj
1.389 0 Td
(cardiovascular)Tj
6.0038 0 Td
(disease)Tj
3.016 0 Td
(by)Tj
1.1792 0 Td
(chronically)Tj
4.6715 0 Td
(activating)Tj
4.1102 0 Td
(the)Tj
-33.7944 -1.2983 Td
(renin-angiotensin-aldosterone)Tj
12.3929 0 Td
(system)Tj
2.9424 0 Td
(\(RAAS\),)Tj
3.5886 0 Td
(increasing)Tj
4.3313 0 Td
(the)Tj
1.4684 0 Td
(secretion)Tj
3.8437 0 Td
(of)Tj
1.0148 0 Td
(catecholamines,)Tj
-29.5821 -1.3039 Td
(and)Tj
1.7291 0 Td
(worsening)Tj
4.3936 0 Td
(the)Tj
1.4741 0 Td
(glucose)Tj
3.1747 0 Td
(and)Tj
1.7292 0 Td
(lipid)Tj
2.0466 0 Td
(metabolisms)Tj
5.261 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(].)Tj
1.6044 0 Td
(Despite)Tj
3.2428 0 Td
(the)Tj
1.4684 0 Td
(hypotensive)Tj
5.0059 0 Td
(effects,)Tj
2.9537 0 Td
(de-)Tj
-34.0835 -1.2983 Td
(creased)Tj
3.1804 0 Td
(sodium)Tj
3.2315 0 Td
(intake)Tj
2.6872 0 Td
(and)Tj
1.7348 0 Td
(RAAS)Tj
2.6816 0 Td
(activation)Tj
4.1555 0 Td
(not)Tj
1.5704 0 Td
(only)Tj
1.9786 0 Td
(impair)Tj
2.897 0 Td
(insulin)Tj
2.9877 0 Td
(sensitivity)Tj
4.2179 0 Td
(and)Tj
1.7291 0 Td
(glucose)Tj
-33.0517 -1.2982 Td
(tolerance)Tj
3.8947 0 Td
([)Tj
0.83 0.64 0.02 0 k
(4)Tj
0 g
(])Tj
1.3777 0 Td
(but)Tj
1.5533 0 Td
(also)Tj
1.7632 0 Td
(lower)Tj
2.4548 0 Td
(plasma)Tj
3.033 0 Td
(high-density)Tj
5.2384 0 Td
(lipoprotein)Tj
4.6885 0 Td
(cholesterol)Tj
4.5581 0 Td
(\(HDL-C\))Tj
3.9571 0 Td
(and)Tj
1.7291 0 Td
(apolipo-)Tj
-34.2479 -1.304 Td
(protein)Tj
3.1521 0 Td
(AI)Tj
1.2415 0 Td
(levels)Tj
2.3754 0 Td
([)Tj
0.83 0.64 0.02 0 k
(5)Tj
0 g
(].)Tj
1.6044 0 Td
(In)Tj
1.1055 0 Td
(normotensive)Tj
5.7543 0 Td
(\(N\))Tj
1.6328 0 Td
(low-density)Tj
4.8926 0 Td
(lipoprotein-receptor)Tj
8.3734 0 Td
(knockout)Tj
4.0252 0 Td
(\(LDLR)Tj
-34.1572 -1.2982 Td
(KO\))Tj
1.9785 0 Td
(or)Tj
1.0999 0 Td
(apolipoprotein)Tj
6.1454 0 Td
(E)Tj
0.7937 0 Td
(knockout)Tj
4.0252 0 Td
(\(apoE)Tj
2.5852 0 Td
(KO\))Tj
1.9785 0 Td
(mice,)Tj
2.3811 0 Td
(chronic)Tj
3.2882 0 Td
(LS)Tj
1.2359 0 Td
(increases)Tj
3.8324 0 Td
(plasma)Tj
3.0331 0 Td
(lipid)Tj
2.0466 0 Td
(levels,)Tj
-34.4237 -1.2983 Td
(which)Tj
2.6475 0 Td
(favors)Tj
2.6362 0 Td
(aortic)Tj
2.5228 0 Td
(lipid)Tj
2.0466 0 Td
(infiltration)Tj
4.5581 0 Td
([)Tj
0.83 0.64 0.02 0 k
(6)Tj
0 g
(].)Tj
1.6044 0 Td
(In)Tj
1.1055 0 Td
(addition)Tj
3.6 0 Td
(to)Tj
1.0261 0 Td
(stimulating)Tj
4.7509 0 Td
(renal)Tj
2.245 0 Td
(sodium)Tj
3.2315 0 Td
(absorption)Tj
-31.9746 -1.2983 Td
(and)Tj
1.7291 0 Td
(vasoconstriction,)Tj
7.0242 0 Td
(angiotensin)Tj
4.8869 0 Td
(II)Tj
0.9014 0 Td
(\(ANG)Tj
2.6929 0 Td
(II\))Tj
1.2416 0 Td
(triggers)Tj
3.2428 0 Td
(oxidative)Tj
3.8664 0 Td
(stress)Tj
2.3981 0 Td
(and)Tj
1.7291 0 Td
(inflammation,)Tj
-29.7125 -1.3039 Td
(favoring)Tj
3.5546 0 Td
(advanced)Tj
3.9911 0 Td
(glycation)Tj
3.8665 0 Td
(end)Tj
1.7177 0 Td
(products)Tj
3.7588 0 Td
(\(AGE\))Tj
2.8686 0 Td
(generation)Tj
4.5071 0 Td
([)Tj
0.83 0.64 0.02 0 k
(7)Tj
0 g
(].)Tj
-23.0682 -1.2982 Td
(It)Tj
0.8617 0 Td
(is)Tj
0.8447 0 Td
(unknown)Tj
4.0819 0 Td
(whether)Tj
3.4809 0 Td
(a)Tj
0.652 0 Td
(LS-induced)Tj
4.8302 0 Td
(blood)Tj
2.5114 0 Td
(pressure)Tj
3.5773 0 Td
(\(BP\))Tj
2.041 0 Td
(reduction)Tj
4.1159 0 Td
(in)Tj
1.0318 0 Td
(hypertensive)Tj
5.295 0 Td
(\(H\))Tj
-34.52 -1.2983 Td
(animals)Tj
3.3278 0 Td
(circumvents)Tj
5.142 0 Td
(the)Tj
1.4684 0 Td
(arterial)Tj
3.067 0 Td
(wall)Tj
1.8312 0 Td
(lipid)Tj
2.0409 0 Td
(infiltration)Tj
4.5638 0 Td
(brought)Tj
3.4242 0 Td
(about)Tj
2.4945 0 Td
(by)Tj
1.1792 0 Td
(LS-induced)Tj
4.8302 0 Td
(hyper-)Tj
-33.3692 -1.3039 Td
(lipidemia.)Tj
4.2065 0 Td
(Since)Tj
2.3528 0 Td
(dyslipidemia)Tj
5.3291 0 Td
(and)Tj
1.7234 0 Td
(chronic)Tj
3.2825 0 Td
(RAAS)Tj
2.6759 0 Td
(activation)Tj
4.1499 0 Td
(are)Tj
1.44 0 Td
(atherogenic)Tj
4.9039 0 Td
(factors,)Tj
3.1238 0 Td
(the)Tj
1.4626 0 Td
(present)Tj
-34.6504 -1.2983 Td
(study)Tj
2.3867 0 Td
(used)Tj
2.0636 0 Td
(hyperlipidemic)Tj
6.2702 0 Td
(mice)Tj
2.1543 0 Td
(with)Tj
1.9956 0 Td
(renovascular)Tj
5.3234 0 Td
(hypertension)Tj
5.4709 0 Td
(\(ANG)Tj
2.6985 0 Td
(II-dependent)Tj
5.4879 0 Td
(hy-)Tj
-33.8511 -1.2982 Td
(pertension;)Tj
4.7111 0 Td
(2-kidney,)Tj
3.9912 0 Td
(1-clip2K1C\))Tj
6.0491 0 Td
(as)Tj
1.0147 0 Td
(a)Tj
0.6577 0 Td
(model)Tj
2.7439 0 Td
(to)Tj
1.0205 0 Td
(investigate)Tj
4.4617 0 Td
(the)Tj
1.474 0 Td
(effects)Tj
2.7212 0 Td
(of)Tj
1.0148 0 Td
(chronic)Tj
3.2882 0 Td
(LS)Tj
1.2359 0 Td
(on)Tj
-34.384 -1.304 Td
(atherogenesis.)Tj
5.8563 0 Td
(In)Tj
1.1055 0 Td
(this)Tj
1.6781 0 Td
(experimental)Tj
5.4595 0 Td
(model,)Tj
2.965 0 Td
(chronic)Tj
3.2882 0 Td
(LS)Tj
1.2359 0 Td
(elicited)Tj
3.0954 0 Td
(vascular)Tj
3.4752 0 Td
(damage.)Tj
3.549 0 Td
(Despite)Tj
3.2485 0 Td
(its)Tj
-34.9566 -1.2982 Td
(antihypertensive)Tj
6.8371 0 Td
(effect,)Tj
2.5908 0 Td
(the)Tj
1.4684 0 Td
(LS)Tj
1.2359 0 Td
(diet)Tj
1.7347 0 Td
(led)Tj
1.4174 0 Td
(to)Tj
1.0261 0 Td
(higher)Tj
2.8006 0 Td
(plasma)Tj
3.0331 0 Td
(lipid)Tj
2.0466 0 Td
(concentrations,)Tj
6.4062 0 Td
(enhanced)Tj
4.0762 0 Td
(local)Tj
-34.6731 -1.2983 Td
(lipid)Tj
2.0466 0 Td
(infiltration,)Tj
4.7848 0 Td
(increased)Tj
4.0025 0 Td
(carboxymethyl-lysine)Tj
8.8044 0 Td
(\(CML,)Tj
2.8743 0 Td
(an)Tj
1.2075 0 Td
(AGE\),)Tj
2.7496 0 Td
(receptor)Tj
3.5603 0 Td
(for)Tj
1.3833 0 Td
(advanced)Tj
3.9968 0 Td
(gly-)Tj
-35.4101 -1.2983 Td
(cation)Tj
2.6985 0 Td
(end)Tj
1.7178 0 Td
(products)Tj
3.753 0 Td
(\(RAGE\),)Tj
3.7191 0 Td
(and)Tj
1.7291 0 Td
(tumor)Tj
2.7439 0 Td
(necrosis)Tj
3.4809 0 Td
(factor)Tj
2.5455 0 Td
(\(TNF\)-)Tj
/F10 1 Tf
()Tj
/F6 1 Tf
3.6907 0 Td
(levels)Tj
2.3754 0 Td
(and)Tj
1.7348 0 Td
(lower)Tj
2.4492 0 Td
(angiotensin)Tj
-32.6379 -1.3039 Td
(II)Tj
0.9014 0 Td
(type)Tj
1.9105 0 Td
(1\(AT1\))Tj
3.1464 0 Td
(receptor)Tj
3.5547 0 Td
(levels.)Tj
2.6021 0 Td
(Overall,)Tj
3.3676 0 Td
(these)Tj
2.2564 0 Td
(experimental)Tj
5.4594 0 Td
(data)Tj
1.9106 0 Td
(suggest)Tj
3.1237 0 Td
(that)Tj
1.7802 0 Td
(long-term)Tj
4.2463 0 Td
(dietary)Tj
-34.2593 -1.2982 Td
(sodium)Tj
3.2314 0 Td
(restriction)Tj
4.354 0 Td
(brings)Tj
2.7383 0 Td
(about)Tj
2.4944 0 Td
(undesirable)Tj
4.8586 0 Td
(effects)Tj
2.7269 0 Td
(on)Tj
1.2756 0 Td
(some)Tj
2.33 0 Td
(markers)Tj
3.498 0 Td
(of)Tj
1.0148 0 Td
(cardiovascular)Tj
6.0037 0 Td
(disease,)Tj
-34.5257 -1.2983 Td
(thus)Tj
1.9332 0 Td
(impairing)Tj
4.1782 0 Td
(the)Tj
1.4683 0 Td
(efficacy)Tj
3.2202 0 Td
(of)Tj
1.0148 0 Td
(LS)Tj
1.2359 0 Td
(in)Tj
1.0375 0 Td
(lowering)Tj
3.7303 0 Td
(BP)Tj
1.3606 0 Td
(for)Tj
1.3833 0 Td
(preventing)Tj
4.5411 0 Td
(atherosclerotic)Tj
6.1058 0 Td
(heart)Tj
2.2734 0 Td
(disease)Tj
-33.4826 -1.3039 Td
(and)Tj
1.7291 0 Td
(reducing)Tj
3.7757 0 Td
(heart)Tj
2.2734 0 Td
(disease-related)Tj
6.0944 0 Td
(mortality.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 347.0173 cm
BT
/F2 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Materials)Tj
4.5071 0 Td
(and)Tj
1.989 0 Td
(methods)Tj
/F0 1 Tf
-6.4961 -1.4976 Td
(Materials)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 312.0377 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(enzymatic)Tj
4.303 0 Td
(colorimetric)Tj
5.1476 0 Td
(kits)Tj
1.6385 0 Td
(used)Tj
2.0636 0 Td
(for)Tj
1.3833 0 Td
(determining)Tj
5.1817 0 Td
(the)Tj
1.4683 0 Td
(plasma)Tj
3.0331 0 Td
(total)Tj
2.0069 0 Td
(cholesterol)Tj
4.5524 0 Td
(\(TC\),)Tj
2.3981 0 Td
(tri-)Tj
-34.9566 -1.2982 Td
(glyceride)Tj
3.821 0 Td
(\(TG\))Tj
2.228 0 Td
(and)Tj
1.7292 0 Td
(nonesterified)Tj
5.4765 0 Td
(fatty)Tj
1.9899 0 Td
(acid)Tj
1.8765 0 Td
(\(NEFA\))Tj
3.4186 0 Td
(levels)Tj
2.3697 0 Td
(were)Tj
2.126 0 Td
(obtained,)Tj
3.9571 0 Td
(respectively,)Tj
5.108 0 Td
(from)Tj
-34.1005 -1.3039 Td
(Labtest)Tj
3.0784 0 Td
(Brazil,)Tj
2.7552 0 Td
(Roche)Tj
2.7269 0 Td
(Brazil)Tj
2.5285 0 Td
(and)Tj
1.7291 0 Td
(Randox)Tj
3.3279 0 Td
(Clinical)Tj
3.3165 0 Td
(Diagnostic)Tj
4.5241 0 Td
(Solutions)Tj
3.9628 0 Td
(\(Randox)Tj
3.6623 0 Td
(of)Tj
1.0148 0 Td
(Brazil,)Tj
-32.6265 -1.2983 Td
(Ltda\).)Tj
2.5851 0 Td
(Twenty-four-hour)Tj
7.5685 0 Td
(urinary)Tj
3.1918 0 Td
(collection)Tj
4.1102 0 Td
(was)Tj
1.7008 0 Td
(accomplished)Tj
5.6976 0 Td
(in)Tj
1.0374 0 Td
(individual)Tj
4.286 0 Td
(metabolic)Tj
4.1442 0 Td
(cages)Tj
-34.3216 -1.2983 Td
(obtained)Tj
3.7303 0 Td
(from)Tj
2.2054 0 Td
(Beiramar)Tj
3.9231 0 Td
(Indu)Tj
0 Tc
1.491 0.0397 Td
()Tj
-0.004 Tc
0.4535 -0.0397 Td
(stria)Tj
1.9503 0 Td
(e)Tj
0.6463 0 Td
(Come)Tj
0 Tc
2.0069 0.0511 Td
()Tj
-0.004 Tc
0.4025 -0.0511 Td
(rcio,)Tj
2.0182 0 Td
(Ltda,)Tj
2.2394 0 Td
(S)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(o)Tj
1.6384 0 Td
(Paulo,)Tj
2.7213 0 Td
(SP,)Tj
1.4796 0 Td
(Brazil)Tj
2.5285 0 Td
(\(length)Tj
3.0728 0 Td
(x)Tj
0.6859 0 Td
(height)Tj
2.727 0 Td
(x)Tj
-35.9204 -1.2982 Td
(width;)Tj
2.7495 0 Td
(30.5)Tj
1.8709 0 Td
(x)Tj
0.686 0 Td
(35)Tj
1.1735 0 Td
(x)Tj
0.6803 0 Td
(20)Tj
1.1736 0 Td
(cm\).)Tj
2.0295 0 Td
(Twenty-four-hour)Tj
7.5685 0 Td
(urinary)Tj
3.1975 0 Td
(sodium)Tj
3.2258 0 Td
(\(UNa\))Tj
2.8063 0 Td
(measurements)Tj
6.032 0 Td
(were)Tj
2.126 0 Td
(per-)Tj
-35.3194 -1.3039 Td
(formed)Tj
3.1577 0 Td
(using)Tj
2.3868 0 Td
(an)Tj
1.2019 0 Td
(FC)Tj
1.4116 0 Td
(280)Tj
1.6498 0 Td
(flame)Tj
2.4321 0 Td
(spectrophotometer)Tj
7.8122 0 Td
(\(CELM;)Tj
3.4469 0 Td
(S)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(o)Tj
1.6384 0 Td
(Paulo,)Tj
2.7213 0 Td
(SP,)Tj
1.4796 0 Td
(Brazil\).)Tj
3.0955 0 Td
(Animal)Tj
-32.4338 -1.2983 Td
(chow,)Tj
2.5851 0 Td
(containing)Tj
4.5298 0 Td
(either)Tj
2.5285 0 Td
(0.15)Tj
1.8765 0 Td
(or)Tj
1.0941 0 Td
(1.27%)Tj
2.6306 0 Td
(sodium)Tj
3.2315 0 Td
(chloride,)Tj
3.7417 0 Td
(was)Tj
1.6951 0 Td
(obtained)Tj
3.736 0 Td
(from)Tj
2.2054 0 Td
(Harlan)Tj
3.0217 0 Td
(Teklad)Tj
-32.876 -1.2983 Td
(\(Madison,)Tj
4.3199 0 Td
(WI,)Tj
1.7518 0 Td
(USA\).)Tj
2.6816 0 Td
(BP)Tj
1.3606 0 Td
(was)Tj
1.7008 0 Td
(measured)Tj
4.1045 0 Td
(with)Tj
2.0013 0 Td
(an)Tj
1.2018 0 Td
(RTBP)Tj
2.5909 0 Td
(2045)Tj
2.1203 0 Td
(model)Tj
2.7439 0 Td
(coupled)Tj
3.3959 0 Td
(to)Tj
1.0261 0 Td
(an)Tj
1.2019 0 Td
(RTBP)Tj
2.5909 0 Td
(001)Tj
-34.7922 -1.3039 Td
(data)Tj
1.9105 0 Td
(acquisition)Tj
4.6318 0 Td
(and)Tj
1.7291 0 Td
(analysis)Tj
3.3278 0 Td
(system)Tj
2.9367 0 Td
(\(Kent)Tj
2.5058 0 Td
(Scientific)Tj
3.9005 0 Td
(Corporation,)Tj
5.4368 0 Td
(CT,)Tj
1.7178 0 Td
(USA\))Tj
2.4604 0 Td
(using)Tj
2.3868 0 Td
(a)Tj
0.6576 0 Td
(Data)Tj
-33.6016 -1.2983 Td
(Acquisition)Tj
4.8755 0 Td
(System)Tj
3.0557 0 Td
(Laboratory)Tj
4.6432 0 Td
(\(DASYLab\))Tj
4.9039 0 Td
(7.0)Tj
1.4003 0 Td
(version)Tj
3.1521 0 Td
(program)Tj
3.702 0 Td
(\(DASYTEC,)Tj
5.159 0 Td
(National)Tj
3.6907 0 Td
(Instru-)Tj
-34.5824 -1.2982 Td
(ments)Tj
2.6702 0 Td
(Company,)Tj
4.388 0 Td
(NH,)Tj
1.9502 0 Td
(USA\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 139.011 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Animals)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 122.0031 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Homozygous)Tj
5.5502 0 Td
(LDLR)Tj
2.6532 0 Td
(KO)Tj
1.6384 0 Td
(mice,)Tj
2.3811 0 Td
(inbred)Tj
2.8629 0 Td
(on)Tj
1.2756 0 Td
(a)Tj
0.6577 0 Td
(C57BL/6J)Tj
4.0875 0 Td
(background)Tj
5.0286 0 Td
(were)Tj
2.1203 0 Td
(purchased)Tj
4.3313 0 Td
(from)Tj
2.2054 0 Td
(Jack-)Tj
-34.7922 -1.2982 Td
(son)Tj
1.6327 0 Td
(Laboratory)Tj
4.6431 0 Td
(\(Bar)Tj
1.9446 0 Td
(Harbor,)Tj
3.4072 0 Td
(ME,)Tj
1.9049 0 Td
(USA\),)Tj
2.6815 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.6577 0 Td
(breeding)Tj
3.753 0 Td
(colony)Tj
2.9084 0 Td
(was)Tj
1.6951 0 Td
(established)Tj
4.5977 0 Td
(in-house.)Tj
3.9572 0 Td
(Ani-)Tj
-35.5122 -1.3039 Td
(mals)Tj
2.0806 0 Td
(were)Tj
2.1203 0 Td
(housed)Tj
3.0954 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(conventional)Tj
5.4255 0 Td
(animal)Tj
2.9707 0 Td
(facility)Tj
2.897 0 Td
(at)Tj
0.9524 0 Td
(22)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(2C)Tj
3.2768 0 Td
(under)Tj
2.6192 0 Td
(a)Tj
0.652 0 Td
(12-h)Tj
2.0466 0 Td
(light/dark)Tj
4.1839 0 Td
(cycle)Tj
-34.0098 -1.2983 Td
(and)Tj
1.7291 0 Td
(were)Tj
2.1203 0 Td
(fed)Tj
1.4626 0 Td
(pelleted)Tj
3.3222 0 Td
(commercial)Tj
4.9606 0 Td
(chow)Tj
2.3584 0 Td
(ad)Tj
1.1849 0 Td
(libitum)Tj
3.1521 0 Td
(\(Nuvilab)Tj
3.7304 0 Td
(CR1,)Tj
2.2053 0 Td
(S)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(o)Tj
1.6384 0 Td
(Paulo,)Tj
2.7213 0 Td
(Brazil\))Tj
2.8686 0 Td
(with)Tj
2.0013 0 Td
(free)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 422.2488 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Dietary)Tj
3.3307 0 Td
(sodium)Tj
3.3874 0 Td
(restrictio)Tj
3.7063 0 Td
(n)Tj
0.7654 0 Td
(and)Tj
1.8567 0 Td
(atherogenesis)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01770)Tj
4.089 0 Td
(86)Tj
0 g
2.0906 0 Td
(May)Tj
2.0906 0 Td
(8,)Tj
1.0346 0 Td
(2017)Tj
35.7806 0 Td
(2)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
24 0 obj
<>stream
endstream
endobj
25 0 obj
<>stream
endstream
endobj
26 0 obj
<>stream
endstream
endobj
27 0 obj
<>stream
endstream
endobj
28 0 obj
<>stream
endstream
endobj
29 0 obj
<>stream
endstream
endobj
30 0 obj
<>stream
endstream
endobj
31 0 obj
<>stream
endstream
endobj
32 0 obj
<>stream
endstream
endobj
33 0 obj
<>stream
endstream
endobj
34 0 obj
<>stream
BT
66.402 0 Td
(22)Tj
ET
Q
endstream
endobj
35 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 36 0 R/Contents 37 0 R/TrimBox[0 0 612 792]>>
endobj
36 0 obj
[38 0 R 39 0 R 40 0 R 41 0 R 42 0 R 43 0 R 44 0 R]
endobj
38 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref008)>>
endobj
39 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref009)>>
endobj
40 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref010)>>
endobj
41 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref008)>>
endobj
42 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref011)>>
endobj
43 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref012)>>
endobj
44 0 obj
<>/Border[0 0 0]/A 45 0 R>>
endobj
45 0 obj
<>
endobj
37 0 obj
[46 0 R 47 0 R 48 0 R 49 0 R 50 0 R 51 0 R 52 0 R 53 0 R]
endobj
46 0 obj
<>stream
q
0.83 0.64 0.02 0 k
233.2913 517.6063 m
237.9969 517.6063 l
h
f*
242.4756 517.6063 m
247.2378 517.6063 l
h
f*
289.0772 478.6016 m
298.6016 478.6016 l
h
f*
408.6992 426.6142 m
413.4614 426.6142 l
h
f*
296.1638 224.9008 m
305.6882 224.9008 l
h
f*
310.1102 224.9008 m
319.6346 224.9008 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(access)Tj
2.6475 0 Td
(to)Tj
1.0205 0 Td
(drinking)Tj
3.7133 0 Td
(water.)Tj
2.6589 0 Td
(All)Tj
1.3946 0 Td
(determinations)Tj
6.3156 0 Td
(were)Tj
2.126 0 Td
(made)Tj
2.4207 0 Td
(from)Tj
2.2054 0 Td
(blood)Tj
2.5171 0 Td
(samples)Tj
3.3846 0 Td
(\(140)Tj
/F11 1 Tf
1.9842 0 Td
[()]TJ
/F6 1 Tf
(L\))Tj
1.6044 0 Td
(drawn)Tj
-33.9928 -1.3039 Td
(from)Tj
2.1996 0 Td
(the)Tj
1.4627 0 Td
(tail)Tj
1.4627 0 Td
(vein)Tj
1.9048 0 Td
(into)Tj
1.8369 0 Td
(heparinized)Tj
4.9322 0 Td
(micro-hematocrit)Tj
7.3247 0 Td
(capillary)Tj
3.6113 0 Td
(tubes)Tj
2.3301 0 Td
(after)Tj
2.0296 0 Td
(a)Tj
0.6463 0 Td
(12-h)Tj
2.0466 0 Td
(overnight)Tj
4.0762 0 Td
(fast-)Tj
-35.8637 -1.2982 Td
(ing)Tj
1.5023 0 Td
(period.)Tj
3.0557 0 Td
(Blood)Tj
2.5909 0 Td
(samples)Tj
3.3789 0 Td
(were)Tj
2.1259 0 Td
(centrifuged)Tj
4.7849 0 Td
(\(3,000)Tj
2.6872 0 Td
(rpm,)Tj
2.1543 0 Td
(4C,)Tj
1.9729 0 Td
(20)Tj
1.1736 0 Td
(min\),)Tj
2.4207 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.4683 0 Td
(levels)Tj
2.3754 0 Td
(of)Tj
1.0148 0 Td
(plasma)Tj
-34.435 -1.2983 Td
(lipids)Tj
2.3924 0 Td
(\(TC,)Tj
2.0522 0 Td
(TG)Tj
1.5364 0 Td
(and)Tj
1.7235 0 Td
(NEFA\))Tj
3.067 0 Td
(were)Tj
2.109 0 Td
(then)Tj
2.0126 0 Td
(immediately)Tj
5.1874 0 Td
(quantified)Tj
4.3029 0 Td
(via)Tj
1.3606 0 Td
(enzymatic)Tj
4.2973 0 Td
(colorimetric)Tj
5.1307 0 Td
(meth-)Tj
-35.172 -1.2983 Td
(ods.)Tj
1.8425 0 Td
(Plasma)Tj
3.0727 0 Td
(TC)Tj
1.491 0 Td
(and)Tj
1.7291 0 Td
(TG)Tj
1.5477 0 Td
(levels,)Tj
2.6022 0 Td
(hematocrit,)Tj
4.8132 0 Td
(and)Tj
1.7348 0 Td
(body)Tj
2.2167 0 Td
(weight)Tj
2.8856 0 Td
(were)Tj
2.1203 0 Td
(measured)Tj
4.1103 0 Td
(monthly.)Tj
3.8437 0 Td
(Animals)Tj
-34.0098 -1.3039 Td
(were)Tj
2.1203 0 Td
(placed)Tj
2.7949 0 Td
(individually)Tj
4.9889 0 Td
(in)Tj
1.0318 0 Td
(metabolic)Tj
4.1499 0 Td
(cages)Tj
2.3244 0 Td
(with)Tj
1.9956 0 Td
(free)Tj
1.7291 0 Td
(access)Tj
2.6476 0 Td
(to)Tj
1.0261 0 Td
(food)Tj
2.0466 0 Td
(and)Tj
1.7291 0 Td
(drinking)Tj
3.7134 0 Td
(water)Tj
2.4321 0 Td
(for)Tj
-34.7298 -1.2982 Td
(twenty-four-hour)Tj
7.2566 0 Td
(UNa)Tj
2.126 0 Td
(assessments.)Tj
5.1987 0 Td
(Systolic)Tj
3.2541 0 Td
(BP)Tj
1.355 0 Td
(was)Tj
1.7008 0 Td
(assessed)Tj
3.4695 0 Td
(in)Tj
1.0375 0 Td
(conscious)Tj
4.1442 0 Td
(mice)Tj
2.1544 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.6519 0 Td
(stan-)Tj
-34.7411 -1.2983 Td
(dard)Tj
2.0806 0 Td
(tail-cuff)Tj
3.3448 0 Td
(technique)Tj
4.1669 0 Td
(and)Tj
1.7292 0 Td
(an)Tj
1.2018 0 Td
(oscillometric)Tj
5.3802 0 Td
(method.)Tj
3.5489 0 Td
(The)Tj
1.7858 0 Td
(mice)Tj
2.1487 0 Td
(were)Tj
2.1259 0 Td
(mildly)Tj
2.778 0 Td
(warmed)Tj
3.4809 0 Td
(up)Tj
1.2699 0 Td
(for)Tj
1.3833 0 Td
(30)Tj
-36.4249 -1.3039 Td
(min)Tj
1.8538 0 Td
(prior)Tj
2.2564 0 Td
(the)Tj
1.4683 0 Td
(BP)Tj
1.3606 0 Td
(assessment.)Tj
4.8359 0 Td
(Only)Tj
2.2167 0 Td
(BP)Tj
1.3549 0 Td
(measurements)Tj
6.0321 0 Td
(from)Tj
2.2054 0 Td
(resting)Tj
2.9593 0 Td
(animals)Tj
3.3279 0 Td
(were)Tj
2.1203 0 Td
(considered.)Tj
-31.9916 -1.2983 Td
(After)Tj
2.2847 0 Td
(three)Tj
2.262 0 Td
(successive)Tj
4.2463 0 Td
(days)Tj
2.0012 0 Td
(of)Tj
1.0148 0 Td
(mouse)Tj
2.8573 0 Td
(preconditioning)Tj
6.7067 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(measurement)Tj
5.6693 0 Td
(system,)Tj
3.1634 0 Td
(twelve)Tj
2.7553 0 Td
(read-)Tj
-35.4555 -1.2983 Td
(ings)Tj
1.8595 0 Td
(were)Tj
2.1259 0 Td
(recorded)Tj
3.7928 0 Td
(on)Tj
1.2756 0 Td
(two)Tj
1.7121 0 Td
(consecutive)Tj
4.8755 0 Td
(days)Tj
1.9956 0 Td
(and)Tj
1.7348 0 Td
(averaged)Tj
3.753 0 Td
(to)Tj
1.0262 0 Td
(obtain)Tj
2.7779 0 Td
(the)Tj
1.4684 0 Td
(mean)Tj
2.4491 0 Td
(values.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 548.674 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Renovascular)Tj
6.2551 0 Td
(hypertension)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 531.7228 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Renovascular)Tj
5.5728 0 Td
(hypertension)Tj
5.4652 0 Td
(\(2K1C\))Tj
3.1918 0 Td
(was)Tj
1.6951 0 Td
(induced)Tj
3.4696 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
1.0204 0 Td
(methods)Tj
3.685 0 Td
(presented)Tj
4.1159 0 Td
(in)Tj
1.0375 0 Td
(previous)Tj
-33.4429 -1.3039 Td
(studies)Tj
2.9876 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2586 0 Td
(9)Tj
0 g
(].)Tj
1.2586 0 Td
(Briefly,)Tj
3.0784 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.652 0 Td
(dissecting)Tj
4.1612 0 Td
(microscope,)Tj
5.0683 0 Td
(rectangular)Tj
4.7622 0 Td
(silver)Tj
2.3414 0 Td
(clips)Tj
2.0353 0 Td
(\(width)Tj
2.8686 0 Td
(X)Tj
0.8731 0 Td
(length;)Tj
-33.7377 -1.2982 Td
(1.5)Tj
1.3946 0 Td
(mm)Tj
1.8595 0 Td
(X)Tj
0.8731 0 Td
(1.0)Tj
1.3946 0 Td
(mm\))Tj
2.1997 0 Td
(with)Tj
2.0012 0 Td
(rounded)Tj
3.651 0 Td
(edges)Tj
2.4208 0 Td
(and)Tj
1.7291 0 Td
(a)Tj
0.6577 0 Td
(0.076-mm-wide)Tj
6.5876 0 Td
(slit)Tj
1.3947 0 Td
(were)Tj
2.1203 0 Td
(made.)Tj
2.6532 0 Td
(One)Tj
1.9389 0 Td
(hour)Tj
2.1543 0 Td
(before)Tj
-35.0303 -1.2983 Td
(anesthesia,)Tj
4.524 0 Td
(tramadol)Tj
3.8551 0 Td
(chlorhydrate)Tj
5.3348 0 Td
(was)Tj
1.7008 0 Td
(subcutaneously)Tj
6.3665 0 Td
(injected)Tj
3.3902 0 Td
(\(12.5)Tj
2.2111 0 Td
(mg/kg)Tj
2.7949 0 Td
(of)Tj
1.0148 0 Td
(body)Tj
2.2167 0 Td
(weight\))Tj
3.2258 0 Td
(as)Tj
-36.6347 -1.3039 Td
(preemptive)Tj
4.7338 0 Td
(analgesia)Tj
3.8324 0 Td
([)Tj
0.83 0.64 0.02 0 k
(10)Tj
0 g
(].)Tj
2.0806 0 Td
(Ketamine)Tj
4.1046 0 Td
(hydrochloride)Tj
5.9073 0 Td
(\(Ketalar\))Tj
3.7757 0 Td
(\(100)Tj
1.99 0 Td
(mg/kg)Tj
2.7949 0 Td
(of)Tj
1.0148 0 Td
(body)Tj
2.2167 0 Td
(weight\))Tj
3.2258 0 Td
(and)Tj
-35.6766 -1.2983 Td
(xylazine)Tj
3.4695 0 Td
(\(Rompun\))Tj
4.4447 0 Td
(\(10)Tj
1.5081 0 Td
(mg/kg)Tj
2.8006 0 Td
(of)Tj
1.0148 0 Td
(body)Tj
2.2166 0 Td
(weight\))Tj
3.2258 0 Td
(were)Tj
2.1203 0 Td
(then)Tj
2.0183 0 Td
(intraperitoneally)Tj
6.8768 0 Td
(injected)Tj
3.3902 0 Td
(as)Tj
1.0148 0 Td
(anesthe-)Tj
-34.1005 -1.2982 Td
(sia.)Tj
1.508 0 Td
(The)Tj
1.7801 0 Td
(body)Tj
2.2167 0 Td
(temperature)Tj
5.1307 0 Td
(was)Tj
1.6951 0 Td
(maintained)Tj
4.7905 0 Td
(within)Tj
2.812 0 Td
(physiological)Tj
5.4424 0 Td
(limits)Tj
2.4832 0 Td
(using)Tj
2.3867 0 Td
(a)Tj
0.6577 0 Td
(surgical)Tj
3.3108 0 Td
(table)Tj
-34.2139 -1.304 Td
(heated)Tj
2.8516 0 Td
(to)Tj
1.0261 0 Td
(37C.)Tj
2.4548 0 Td
(Celiotomy)Tj
4.405 0 Td
(was)Tj
1.7008 0 Td
(performed)Tj
4.4674 0 Td
(to)Tj
1.0261 0 Td
(place)Tj
2.262 0 Td
(a)Tj
0.6577 0 Td
(silver)Tj
2.3414 0 Td
(clip)Tj
1.6724 0 Td
(around)Tj
3.1408 0 Td
(the)Tj
1.4683 0 Td
(right)Tj
2.1487 0 Td
(renal)Tj
2.245 0 Td
(artery,)Tj
-33.8681 -1.2982 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(emergence)Tj
4.541 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(renal)Tj
2.245 0 Td
(artery)Tj
2.5738 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(aorta)Tj
2.2677 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.5988 0 Td
(Subcutaneous)Tj
5.7826 0 Td
(tramadol)Tj
3.8608 0 Td
(chlorhydrate)Tj
-31.7705 -1.2983 Td
(injections)Tj
4.1215 0 Td
(\(12.5)Tj
2.211 0 Td
(mg/kg)Tj
2.795 0 Td
(of)Tj
1.0147 0 Td
(body)Tj
2.2167 0 Td
(weight\))Tj
3.2258 0 Td
(were)Tj
2.126 0 Td
(administered)Tj
5.4878 0 Td
(every)Tj
2.3471 0 Td
(8)Tj
0.6973 0 Td
(h)Tj
0.7484 0 Td
(for)Tj
1.3833 0 Td
(48)Tj
1.1735 0 Td
(h)Tj
0.7484 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4684 0 Td
(surgical)Tj
-33.8001 -1.2983 Td
(procedure.)Tj
4.5354 0 Td
(Tramadol)Tj
4.1725 0 Td
(chlorhydrate)Tj
5.3348 0 Td
(\(12.5)Tj
2.211 0 Td
(mg/kg)Tj
2.7949 0 Td
(of)Tj
1.0148 0 Td
(body)Tj
2.2167 0 Td
(weight\))Tj
3.2258 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0183 0 Td
(administered)Tj
5.4878 0 Td
(into)Tj
-34.7128 -1.3039 Td
(the)Tj
1.4683 0 Td
(drinking)Tj
3.7133 0 Td
(water)Tj
2.4322 0 Td
(daily)Tj
2.1486 0 Td
(for)Tj
1.3833 0 Td
(up)Tj
1.2643 0 Td
(to)Tj
1.0261 0 Td
(one-week)Tj
4.0818 0 Td
(after)Tj
2.0296 0 Td
(the)Tj
1.474 0 Td
(48-h)Tj
2.0467 0 Td
(post-surgical)Tj
5.3517 0 Td
(period.)Tj
3.0614 0 Td
(Enrofloxacin)Tj
-31.4813 -1.2982 Td
(\(1)Tj
1.0318 0 Td
(mL/40)Tj
2.8629 0 Td
(kg)Tj
1.1792 0 Td
(of)Tj
1.0148 0 Td
(body)Tj
2.2167 0 Td
(weight\))Tj
3.2258 0 Td
(was)Tj
1.7008 0 Td
(subcutaneously)Tj
6.3666 0 Td
(administered)Tj
5.4878 0 Td
(once)Tj
2.126 0 Td
(a)Tj
0.6519 0 Td
(day)Tj
1.6385 0 Td
(for)Tj
1.3833 0 Td
(3)Tj
0.6973 0 Td
(days.)Tj
2.2223 0 Td
(None)Tj
2.4378 0 Td
(of)Tj
-36.2435 -1.2983 Td
(the)Tj
1.4683 0 Td
(animals)Tj
3.3278 0 Td
(became)Tj
3.2542 0 Td
(ill)Tj
0.9808 0 Td
(or)Tj
1.0941 0 Td
(died)Tj
1.9673 0 Td
(prior)Tj
2.2563 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(experimental)Tj
5.4595 0 Td
(endpoint.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 333.9779 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Morphometric)Tj
6.2693 0 Td
(studies)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 317.0267 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(After)Tj
2.2847 0 Td
(the)Tj
1.4683 0 Td
(mice)Tj
2.1543 0 Td
(were)Tj
2.1203 0 Td
(sacrificed,)Tj
4.2349 0 Td
(the)Tj
1.4684 0 Td
(heart)Tj
2.2734 0 Td
(and)Tj
1.7348 0 Td
(thoracic)Tj
3.4695 0 Td
(aorta)Tj
2.2621 0 Td
(were)Tj
2.1259 0 Td
(dissected)Tj
3.8325 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.6519 0 Td
(stereoscope)Tj
-32.4734 -1.3039 Td
(for)Tj
1.3832 0 Td
(magnification.)Tj
6.0151 0 Td
(The)Tj
1.7802 0 Td
(mice)Tj
2.1543 0 Td
(were)Tj
2.126 0 Td
(then)Tj
2.0182 0 Td
(transcardially)Tj
5.6353 0 Td
(perfused)Tj
3.6623 0 Td
(under)Tj
2.6192 0 Td
(low-pressure,)Tj
5.5899 0 Td
(first)Tj
1.8028 0 Td
(with)Tj
1.9956 0 Td
(a)Tj
-36.7821 -1.2982 Td
(cold)Tj
1.9218 0 Td
(0.9%)Tj
2.1543 0 Td
(NaCl)Tj
2.2961 0 Td
(solution)Tj
3.4866 0 Td
(and)Tj
1.7291 0 Td
(thereafter)Tj
4.0762 0 Td
(with)Tj
1.9956 0 Td
(tissue-freezing)Tj
6.0094 0 Td
(medium)Tj
3.6056 0 Td
(to)Tj
1.0262 0 Td
(ensure)Tj
2.8743 0 Td
(the)Tj
1.4683 0 Td
(proper)Tj
2.9254 0 Td
(pres-)Tj
-35.5689 -1.2983 Td
(ervation)Tj
3.5206 0 Td
(of)Tj
1.0148 0 Td
(histological)Tj
4.7735 0 Td
(structures)Tj
4.1839 0 Td
(for)Tj
1.3833 0 Td
(frozen-tissue)Tj
5.3631 0 Td
(specimens.)Tj
4.5921 0 Td
(The)Tj
1.7801 0 Td
(heart)Tj
2.2734 0 Td
(and)Tj
1.7291 0 Td
(thoracic)Tj
3.4753 0 Td
(aorta)Tj
-34.0892 -1.3039 Td
(were)Tj
2.1203 0 Td
(then)Tj
2.0182 0 Td
(excised)Tj
3.1124 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(fresh)Tj
2.1884 0 Td
(state)Tj
2.0352 0 Td
(and)Tj
1.7348 0 Td
(preserved)Tj
4.0932 0 Td
(in)Tj
1.0318 0 Td
(liquid)Tj
2.5625 0 Td
(nitrogen)Tj
3.651 0 Td
(for)Tj
1.389 0 Td
(future)Tj
2.6532 0 Td
(histological)Tj
-31.0958 -1.2983 Td
(evaluation.)Tj
4.5864 0 Td
(Cryostat)Tj
3.6056 0 Td
(\(Leica)Tj
2.6476 0 Td
(CM1800)Tj
3.6793 0 Td
(model,)Tj
2.9707 0 Td
(MD,)Tj
2.0693 0 Td
(USA\))Tj
2.4604 0 Td
(serial)Tj
2.3188 0 Td
(cross-sections)Tj
5.7883 0 Td
(4)Tj
/F11 1 Tf
0.6916 0 Td
[()]TJ
/F6 1 Tf
(m)Tj
1.5477 0 Td
(in)Tj
1.0375 0 Td
(thickness)Tj
-33.4032 -1.2982 Td
(were)Tj
2.1203 0 Td
(obtained)Tj
3.736 0 Td
(from)Tj
2.2053 0 Td
(an)Tj
1.2019 0 Td
(aortic)Tj
2.5228 0 Td
(arch)Tj
1.9729 0 Td
(segment)Tj
3.5603 0 Td
(10)Tj
1.1736 0 Td
(mm)Tj
1.8595 0 Td
(in)Tj
1.0318 0 Td
(length,)Tj
2.9593 0 Td
(beginning)Tj
4.2576 0 Td
(from)Tj
2.2054 0 Td
(the)Tj
1.4683 0 Td
(aortic)Tj
2.5228 0 Td
(root.)Tj
-34.7978 -1.304 Td
(As)Tj
1.2642 0 Td
(previously)Tj
4.3483 0 Td
(reported)Tj
3.6624 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.7347 0 Td
(12)Tj
0 g
(],)Tj
1.7405 0 Td
(early)Tj
2.1487 0 Td
(atherosclerotic)Tj
6.1001 0 Td
(lesion)Tj
2.5738 0 Td
(development)Tj
5.4142 0 Td
(occurs)Tj
2.8289 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(inner)Tj
-34.3216 -1.2982 Td
(curvature)Tj
4.0535 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(aortic)Tj
2.5172 0 Td
(arch)Tj
1.9785 0 Td
(and)Tj
1.7291 0 Td
(at)Tj
0.9525 0 Td
(the)Tj
1.474 0 Td
(beginning)Tj
4.2576 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(side)Tj
1.8029 0 Td
(branches,)Tj
4.0421 0 Td
(which)Tj
2.6476 0 Td
(are)Tj
1.4513 0 Td
(natural)Tj
3.0784 0 Td
(sites)Tj
-34.9509 -1.2983 Td
(of)Tj
1.0147 0 Td
(shear)Tj
2.3358 0 Td
(stress-related)Tj
5.4708 0 Td
(plaque)Tj
2.8743 0 Td
(initiation.)Tj
4.1499 0 Td
(Variations)Tj
4.405 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(blood)Tj
2.5115 0 Td
(flow)Tj
1.9446 0 Td
(patterns)Tj
3.4752 0 Td
(in)Tj
1.0318 0 Td
(different)Tj
3.6567 0 Td
(vas-)Tj
-35.3761 -1.3039 Td
(cular)Tj
2.2223 0 Td
(regions)Tj
3.1634 0 Td
(have)Tj
2.058 0 Td
(complex)Tj
3.6226 0 Td
(effects)Tj
2.7269 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4684 0 Td
(development)Tj
5.4084 0 Td
(of)Tj
1.0148 0 Td
(atherosclerosis.)Tj
6.3326 0 Td
(With)Tj
2.279 0 Td
(the)Tj
1.4684 0 Td
(aim)Tj
1.7404 0 Td
(of)Tj
-34.7808 -1.2983 Td
(facilitating)Tj
4.456 0 Td
(the)Tj
1.4683 0 Td
(time-consuming)Tj
6.8485 0 Td
(vascular)Tj
3.4752 0 Td
(cross-sectional)Tj
6.1002 0 Td
(analysis,)Tj
3.5546 0 Td
(four)Tj
1.9105 0 Td
(aortic)Tj
2.5228 0 Td
(arch)Tj
1.9786 0 Td
(segments)Tj
-32.3147 -1.2982 Td
(\(named)Tj
3.3108 0 Td
(segments)Tj
3.9231 0 Td
(I,)Tj
0.7824 0 Td
(II,)Tj
1.1282 0 Td
(III)Tj
1.2415 0 Td
(and)Tj
1.7291 0 Td
(IV;)Tj
1.4797 0 Td
(each)Tj
2.0296 0 Td
(140)Tj
/F11 1 Tf
1.6441 0 Td
[()]TJ
/F6 1 Tf
(m)Tj
1.5477 0 Td
(long\))Tj
2.3301 0 Td
(with)Tj
1.9956 0 Td
(1-mm)Tj
2.6872 0 Td
(intervals)Tj
3.6226 0 Td
(between)Tj
3.5263 0 Td
(them)Tj
2.2904 0 Td
(were)Tj
-35.2684 -1.2983 Td
(investigated.)Tj
5.2157 0 Td
(Aortic)Tj
2.7666 0 Td
(arch)Tj
1.9785 0 Td
(segments)Tj
3.9232 0 Td
(III)Tj
1.2359 0 Td
(and)Tj
1.7347 0 Td
(IV)Tj
1.253 0 Td
(were)Tj
2.1203 0 Td
(located)Tj
3.084 0 Td
(closer)Tj
2.5512 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(heart)Tj
2.2733 0 Td
(than)Tj
2.0296 0 Td
(were)Tj
2.1203 0 Td
(seg-)Tj
-34.7808 -1.3039 Td
(ments)Tj
2.6702 0 Td
(I)Tj
0.5612 0 Td
(and)Tj
1.7291 0 Td
(II.)Tj
1.1226 0 Td
(Three)Tj
2.5795 0 Td
(sets)Tj
1.6667 0 Td
(of)Tj
1.0148 0 Td
(10)Tj
1.1679 0 Td
(cryostat)Tj
3.3675 0 Td
(cross-sections)Tj
5.7827 0 Td
(\(each)Tj
2.3697 0 Td
(4-)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(m)Tj
2.3754 0 Td
(thick\),)Tj
2.795 0 Td
(at)Tj
0.9524 0 Td
(100-)Tj
/F11 1 Tf
[()]TJ
/F6 1 Tf
(m)Tj
3.3279 0 Td
(intervals)Tj
-33.4826 -1.2983 Td
(between)Tj
3.5262 0 Td
(each)Tj
2.0296 0 Td
(set,)Tj
1.5307 0 Td
(were)Tj
2.1203 0 Td
(serially)Tj
3.0217 0 Td
(obtained)Tj
3.7361 0 Td
(from)Tj
2.2053 0 Td
(segments)Tj
3.9175 0 Td
(I,)Tj
0.788 0 Td
(II,)Tj
1.1282 0 Td
(III)Tj
1.2359 0 Td
(and)Tj
1.7348 0 Td
(IV,)Tj
1.4796 0 Td
(yielding)Tj
3.4186 0 Td
(120)Tj
1.6441 0 Td
(aortic)Tj
-33.5166 -1.2982 Td
(cross-section)Tj
5.4141 0 Td
(samples)Tj
3.3789 0 Td
(from)Tj
2.1996 0 Td
(each)Tj
2.024 0 Td
(animal.)Tj
3.1918 0 Td
(Fifteen)Tj
2.9933 0 Td
(cross-sections)Tj
5.777 0 Td
(of)Tj
1.0091 0 Td
(each)Tj
2.0239 0 Td
(segment)Tj
3.5547 0 Td
(were)Tj
2.1203 0 Td
(randomly)Tj
-33.6867 -1.304 Td
(chosen)Tj
3.0103 0 Td
(for)Tj
1.3833 0 Td
(histological)Tj
4.7735 0 Td
(analysis.)Tj
3.549 0 Td
(Thus,)Tj
2.4775 0 Td
(sixty)Tj
2.0636 0 Td
(samples)Tj
3.3845 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(aortic)Tj
2.5229 0 Td
(arch)Tj
1.9729 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(animal)Tj
2.9706 0 Td
(were)Tj
-33.6356 -1.2982 Td
(microscopically)Tj
6.4856 0 Td
(analyzed.)Tj
3.9288 0 Td
(Alternate)Tj
3.9458 0 Td
(sections)Tj
3.4129 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(segment)Tj
3.5602 0 Td
(were)Tj
2.1204 0 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(lipid)Tj
2.0466 0 Td
(content)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 422.2488 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Dietary)Tj
3.3307 0 Td
(sodium)Tj
3.3874 0 Td
(restrictio)Tj
3.7063 0 Td
(n)Tj
0.7654 0 Td
(and)Tj
1.8567 0 Td
(atherogenesis)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01770)Tj
4.089 0 Td
(86)Tj
0 g
2.0906 0 Td
(May)Tj
2.0906 0 Td
(8,)Tj
1.0346 0 Td
(2017)Tj
35.7806 0 Td
(3)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
47 0 obj
<>stream
endstream
endobj
48 0 obj
<>stream
endstream
endobj
49 0 obj
<>stream
endstream
endobj
50 0 obj
<>stream
endstream
endobj
51 0 obj
<>stream
endstream
endobj
52 0 obj
<>stream
endstream
endobj
53 0 obj
<>stream
BT
66.402 0 Td
(22)Tj
ET
Q
endstream
endobj
54 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 57 0 R/Contents 58 0 R/TrimBox[0 0 612 792]>>
endobj
57 0 obj
[59 0 R 60 0 R]
endobj
59 0 obj
<>/Border[0 0 0]/Dest(Rpone.0177086.ref013)>>
endobj
60 0 obj
<>/Border[0 0 0]/A 61 0 R>>
endobj
61 0 obj
<>
endobj
58 0 obj
[62 0 R 63 0 R 64 0 R 65 0 R 66 0 R]
endobj
62 0 obj
<>stream
q
0.83 0.64 0.02 0 k
350.022 680.315 m
359.5465 680.315 l
h
f*
0 g
1 j
1 J
0 w
10 0 0 10 200.0125 707.4141 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(and)Tj
1.7291 0 Td
(immunofluorescence)Tj
8.6796 0 Td
(analyses.)Tj
3.7077 0 Td
(To)Tj
1.3379 0 Td
(analyze)Tj
3.1692 0 Td
(lipid)Tj
2.0466 0 Td
(infiltration)Tj
4.5637 0 Td
(into)Tj
1.8425 0 Td
(the)Tj
1.4684 0 Td
(aortic)Tj
2.5228 0 Td
(arch)Tj
1.9729 0 Td
(wall,)Tj
2.0579 0 Td
(sam-)Tj
-35.0983 -1.3039 Td
(ples)Tj
1.7688 0 Td
(were)Tj
2.1203 0 Td
(stained)Tj
3.0784 0 Td
(with)Tj
2.0012 0 Td
(oil)Tj
1.2359 0 Td
(red)Tj
1.542 0 Td
(O)Tj
0.9695 0 Td
(and)Tj
1.7291 0 Td
(counterstained)Tj
6.1795 0 Td
(with)Tj
2.0012 0 Td
(hematoxylin,)Tj
5.4369 0 Td
(according)Tj
4.1839 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4683 0 Td
(modi-)Tj
-34.7411 -1.2982 Td
(fied)Tj
1.7234 0 Td
(method)Tj
3.3279 0 Td
(reported)Tj
3.6566 0 Td
(by)Tj
1.1849 0 Td
(Paigen)Tj
/F12 1 Tf
2.914 0 Td
[()8()]TJ
0.9127 0 Td
[()11()]TJ
/F6 1 Tf
0.9411 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 654.9165 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Immunofluorescenc)Tj
8.6315 0 Td
(e)Tj
0.7701 0 Td
(assays)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 637.9086 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(AT1)Tj
1.9899 0 Td
(receptor,)Tj
3.7814 0 Td
(CML)Tj
2.3187 0 Td
(and)Tj
1.7291 0 Td
(RAGE)Tj
2.8063 0 Td
(contents)Tj
3.634 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.4684 0 Td
(aortic)Tj
2.5228 0 Td
(arch)Tj
1.9786 0 Td
(wall)Tj
1.8311 0 Td
(were)Tj
2.1203 0 Td
(evaluated)Tj
3.9912 0 Td
(by)Tj
1.1792 0 Td
(immu-)Tj
-34.1629 -1.2982 Td
(nofluorescence.)Tj
6.4686 0 Td
(Specimens)Tj
4.4787 0 Td
(were)Tj
2.1203 0 Td
(mounted)Tj
3.8721 0 Td
(on)Tj
1.2756 0 Td
(glass)Tj
2.0863 0 Td
(slides)Tj
2.4094 0 Td
(with)Tj
1.9956 0 Td
(3-aminopropyltrietho)Tj
8.7136 0 Td
(xysilane)Tj
-33.4202 -1.2983 Td
(\(Sigma)Tj
3.0217 0 Td
(Chemical,)Tj
4.2462 0 Td
(Co.\),)Tj
2.1827 0 Td
(washed)Tj
3.1805 0 Td
(in)Tj
1.0318 0 Td
(phosphate-buffered)Tj
8.0446 0 Td
(saline)Tj
2.5002 0 Td
(\(PBS\))Tj
2.5115 0 Td
(and)Tj
1.7347 0 Td
(blocked)Tj
3.3506 0 Td
(with)Tj
1.9956 0 Td
(5%)Tj
-33.8001 -1.2982 Td
(bovine)Tj
2.9196 0 Td
(serum)Tj
2.7099 0 Td
(albumin)Tj
3.566 0 Td
(in)Tj
1.0261 0 Td
(PBS)Tj
1.8255 0 Td
(for)Tj
1.3776 0 Td
(30)Tj
1.1622 0 Td
(min)Tj
1.8482 0 Td
(at)Tj
0.9411 0 Td
(room)Tj
2.4151 0 Td
(temperature.)Tj
5.3461 0 Td
(The)Tj
1.7745 0 Td
(slices)Tj
2.2904 0 Td
(were)Tj
2.1146 0 Td
(incubated)Tj
4.1726 0 Td
(over-)Tj
-35.4895 -1.304 Td
(night)Tj
2.3243 0 Td
(at)Tj
0.9525 0 Td
(4C)Tj
1.7518 0 Td
(with)Tj
1.9956 0 Td
(rabbit)Tj
2.6078 0 Td
(polyclonal)Tj
4.3483 0 Td
(anti-AT1)Tj
3.8891 0 Td
(receptor)Tj
3.5547 0 Td
(\(1:40;)Tj
2.4377 0 Td
(Santa)Tj
2.4151 0 Td
(Cruz)Tj
2.1941 0 Td
(Biotechnology,)Tj
6.2021 0 Td
(Inc.,)Tj
-34.6731 -1.2982 Td
(Cat)Tj
1.6157 0 Td
(#)Tj
0.6916 0 Td
(SC1173,)Tj
3.4866 0 Td
(Dallas,)Tj
2.9084 0 Td
(TX,)Tj
1.7064 0 Td
(USA\),)Tj
2.6816 0 Td
(rabbit)Tj
2.6021 0 Td
(polyclonal)Tj
4.354 0 Td
(anti-CML)Tj
4.2123 0 Td
(\(1:40;)Tj
2.4378 0 Td
(Immundiagnostik)Tj
7.4494 0 Td
(AG,)Tj
1.8425 0 Td
(Cat)Tj
-35.9884 -1.2983 Td
(#)Tj
0.6916 0 Td
(1109,)Tj
2.3527 0 Td
(Bensheim,)Tj
4.3937 0 Td
(Germany\))Tj
4.3257 0 Td
(and)Tj
1.7347 0 Td
(rabbit)Tj
2.6022 0 Td
(polyclonal)Tj
4.3484 0 Td
(anti-RAGE)Tj
4.7111 0 Td
(\(1:50;)Tj
2.4378 0 Td
(Santa)Tj
2.4094 0 Td
(Cruz)Tj
2.194 0 Td
(Biotechnol-)Tj
-32.2013 -1.3039 Td
(ogy,)Tj
1.8708 0 Td
(Inc.,)Tj
1.9842 0 Td
(Cat)Tj
1.6158 0 Td
(#)Tj
0.6916 0 Td
(SC)Tj
1.3607 0 Td
(5663,)Tj
2.347 0 Td
(Dallas,)Tj
2.9027 0 Td
(TX,)Tj
1.7121 0 Td
(USA\))Tj
2.4548 0 Td
(antibodies)Tj
4.3596 0 Td
(diluted)Tj
3.0388 0 Td
(in)Tj
1.0374 0 Td
(PBS.)Tj
2.058 0 Td
(The)Tj
1.7858 0 Td
(sections)Tj
3.4072 0 Td
(were)Tj
2.126 0 Td
(next)Tj
-34.7525 -1.2983 Td
(washed)Tj
3.1747 0 Td
(in)Tj
1.0375 0 Td
(PBS)Tj
1.8368 0 Td
(with)Tj
1.9956 0 Td
(0.05%)Tj
2.6306 0 Td
(Tween-20)Tj
4.2122 0 Td
(and)Tj
1.7291 0 Td
(incubated)Tj
4.1783 0 Td
(for)Tj
1.3833 0 Td
(90)Tj
1.1735 0 Td
(min)Tj
1.8539 0 Td
(at)Tj
0.9524 0 Td
(room)Tj
2.4264 0 Td
(temperature)Tj
5.1307 0 Td
(with)Tj
-33.715 -1.2982 Td
(Alexa)Tj
2.4717 0 Td
(488-conjugated)Tj
6.412 0 Td
(goat)Tj
1.9219 0 Td
(anti-rabbit)Tj
4.5013 0 Td
(IgG)Tj
1.7405 0 Td
(antibody)Tj
3.7644 0 Td
(\(Life)Tj
2.0806 0 Td
(Technologies,)Tj
5.7543 0 Td
(Cat)Tj
1.6157 0 Td
(#)Tj
0.6974 0 Td
(A11008,)Tj
3.5036 0 Td
(Grand)Tj
-34.4634 -1.304 Td
(Island,)Tj
2.9026 0 Td
(NY,)Tj
1.8142 0 Td
(USA\))Tj
2.4548 0 Td
(diluted)Tj
3.0387 0 Td
(at)Tj
0.9524 0 Td
(1:200)Tj
2.3471 0 Td
(in)Tj
1.0375 0 Td
(a)Tj
0.6519 0 Td
(PBS)Tj
1.8369 0 Td
(solution)Tj
3.4809 0 Td
(containing)Tj
4.5297 0 Td
(0.006%)Tj
3.1011 0 Td
(Evans)Tj
2.5909 0 Td
(blue)Tj
1.9218 0 Td
(dye.)Tj
1.8539 0 Td
(Finally,)Tj
-34.5144 -1.2982 Td
(the)Tj
1.4683 0 Td
(samples)Tj
3.3845 0 Td
(were)Tj
2.1203 0 Td
(mounted)Tj
3.8721 0 Td
(in)Tj
1.0375 0 Td
(buffered)Tj
3.5773 0 Td
(glycerol)Tj
3.3562 0 Td
(and)Tj
1.7291 0 Td
(analyzed)Tj
3.7021 0 Td
(using)Tj
2.3924 0 Td
(fluorescence)Tj
5.1874 0 Td
(microscopy)Tj
-31.8272 -1.2983 Td
(\(Olympus)Tj
4.2349 0 Td
(BX51,)Tj
2.6248 0 Td
(Olympus,)Tj
4.1216 0 Td
(Co.,)Tj
1.8425 0 Td
(Tokyo,)Tj
3.0217 0 Td
(Japan\).)Tj
3.0444 0 Td
(For)Tj
1.6271 0 Td
(negative)Tj
3.5376 0 Td
(and)Tj
1.7291 0 Td
(autofluorescence)Tj
6.9505 0 Td
(controls,)Tj
-32.7342 -1.3039 Td
(sections)Tj
3.4072 0 Td
(were)Tj
2.1259 0 Td
(incubated)Tj
4.1726 0 Td
(with)Tj
2.0013 0 Td
(PBS)Tj
1.8311 0 Td
(instead)Tj
3.0841 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(specific)Tj
3.1918 0 Td
(antibody.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 442.4881 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Histomorphometric)Tj
8.5181 0 Td
(quantification)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 425.4803 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(The)Tj
1.7801 0 Td
(extent)Tj
2.6759 0 Td
(of)Tj
1.0148 0 Td
(lipid)Tj
2.0466 0 Td
(infiltration)Tj
4.5637 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.4683 0 Td
(AT1)Tj
1.9899 0 Td
(receptor,)Tj
3.7814 0 Td
(CML)Tj
2.3187 0 Td
(and)Tj
1.7291 0 Td
(RAGE)Tj
2.812 0 Td
(contents)Tj
3.6283 0 Td
(in)Tj
1.0318 0 Td
(the)Tj
1.474 0 Td
(suben-)Tj
-34.0438 -1.3039 Td
(dothelial)Tj
3.6963 0 Td
(space)Tj
2.3811 0 Td
(and)Tj
1.7291 0 Td
(media)Tj
2.6929 0 Td
(layer)Tj
2.1487 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(aortic)Tj
2.5228 0 Td
(arch)Tj
1.9729 0 Td
(tissue)Tj
2.4605 0 Td
(were)Tj
2.1259 0 Td
(evaluated)Tj
3.9855 0 Td
(in)Tj
1.0375 0 Td
(each)Tj
2.0296 0 Td
(histological)Tj
4.7678 0 Td
(sec-)Tj
-36.0337 -1.2982 Td
(tion)Tj
1.8425 0 Td
(utilizing)Tj
3.5149 0 Td
(an)Tj
1.2075 0 Td
(image)Tj
2.6249 0 Td
(analysis)Tj
3.3279 0 Td
(system)Tj
2.9366 0 Td
(by)Tj
1.1849 0 Td
(an)Tj
1.2019 0 Td
(independent)Tj
5.2724 0 Td
(and)Tj
1.7348 0 Td
(experienced)Tj
5.0343 0 Td
(field)Tj
1.9729 0 Td
(investigator)Tj
-31.8555 -1.2983 Td
(blinded)Tj
3.2654 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(study)Tj
2.3924 0 Td
(protocol.)Tj
3.8211 0 Td
(Briefly,)Tj
3.0784 0 Td
(the)Tj
1.4684 0 Td
(equipment)Tj
4.5524 0 Td
(consisted)Tj
3.9344 0 Td
(of)Tj
1.0148 0 Td
(an)Tj
1.2076 0 Td
(Olympus)Tj
3.8891 0 Td
(camera)Tj
3.1294 0 Td
(\(Olym-)Tj
-34.2479 -1.2983 Td
(pus,)Tj
1.8481 0 Td
(Co.,)Tj
1.8425 0 Td
(St.)Tj
1.2189 0 Td
(Laurent,)Tj
3.5887 0 Td
(Quebec,)Tj
3.4979 0 Td
(Canada\))Tj
3.6 0 Td
(coupled)Tj
3.3902 0 Td
(to)Tj
1.0261 0 Td
(an)Tj
1.2019 0 Td
(Olympus)Tj
3.8948 0 Td
(microscope)Tj
4.8415 0 Td
(\(Olympus)Tj
4.235 0 Td
(BX51,)Tj
-34.1856 -1.3039 Td
(Olympus,)Tj
4.1158 0 Td
(Co.,)Tj
1.8425 0 Td
(Tokyo,)Tj
3.0218 0 Td
(Japan\),)Tj
3.05 0 Td
(from)Tj
2.2054 0 Td
(which)Tj
2.6475 0 Td
(the)Tj
1.474 0 Td
(images)Tj
2.9877 0 Td
(were)Tj
2.1203 0 Td
(sent)Tj
1.8538 0 Td
(to)Tj
1.0262 0 Td
(an)Tj
1.2019 0 Td
(LG)Tj
1.474 0 Td
(monitor)Tj
3.5376 0 Td
(via)Tj
1.3776 0 Td
(a)Tj
0.652 0 Td
(digitiz-)Tj
-34.5881 -1.2982 Td
(ing)Tj
1.5023 0 Td
(system)Tj
2.9367 0 Td
(\(Oculus)Tj
3.3902 0 Td
(TCX,)Tj
2.3754 0 Td
(Coreco,)Tj
3.3392 0 Td
(Inc.,)Tj
1.9842 0 Td
(St.)Tj
1.2189 0 Td
(Laurent,)Tj
3.5887 0 Td
(Quebec,)Tj
3.4979 0 Td
(Canada\))Tj
3.6 0 Td
(and)Tj
1.7291 0 Td
(downloaded)Tj
5.1534 0 Td
(to)Tj
1.0261 0 Td
(a)Tj
-35.3421 -1.2983 Td
(computer)Tj
4.1045 0 Td
(\(Pentium)Tj
4.0082 0 Td
(1330)Tj
2.1203 0 Td
(MHz\).)Tj
2.8573 0 Td
(The)Tj
1.7858 0 Td
(images)Tj
2.9877 0 Td
(were)Tj
2.1259 0 Td
(then)Tj
2.0183 0 Td
(processed)Tj
4.1329 0 Td
(with)Tj
1.9956 0 Td
(Image-Pro-Plus)Tj
6.5366 0 Td
(6.0)Tj
-34.6731 -1.3039 Td
(software.)Tj
3.8097 0 Td
(To)Tj
1.3379 0 Td
(quantify)Tj
3.549 0 Td
(lipid)Tj
2.0409 0 Td
(infiltration,)Tj
4.7905 0 Td
(the)Tj
1.4684 0 Td
(CML)Tj
2.313 0 Td
(content)Tj
3.2712 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(AT1)Tj
1.9899 0 Td
(receptor)Tj
3.5546 0 Td
(and)Tj
1.7348 0 Td
(RAGE)Tj
-33.0574 -1.2983 Td
(expression)Tj
4.4617 0 Td
(levels)Tj
2.3754 0 Td
(in)Tj
1.0374 0 Td
(the)Tj
1.4684 0 Td
(cross-sectional)Tj
6.1058 0 Td
(areas,)Tj
2.4661 0 Td
(digital)Tj
2.7269 0 Td
(images)Tj
2.9934 0 Td
(of)Tj
1.0148 0 Td
(either)Tj
2.5285 0 Td
(positively)Tj
4.0195 0 Td
(stained)Tj
3.0784 0 Td
(or)Tj
-34.2763 -1.2983 Td
(immunostained)Tj
6.5706 0 Td
(sections)Tj
3.4072 0 Td
(were)Tj
2.1203 0 Td
(obtained)Tj
3.7361 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
1.4684 0 Td
(level)Tj
2.0125 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(aortic)Tj
2.5172 0 Td
(arch)Tj
1.9786 0 Td
(\(from)Tj
2.5455 0 Td
(the)Tj
1.4683 0 Td
(intima)Tj
2.8573 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
-35.1493 -1.3039 Td
(external)Tj
3.4298 0 Td
(elastic)Tj
2.6759 0 Td
(lamina\).)Tj
3.532 0 Td
(Care)Tj
2.1146 0 Td
(was)Tj
1.6951 0 Td
(taken)Tj
2.4208 0 Td
(to)Tj
1.0261 0 Td
(exclude)Tj
3.2599 0 Td
(normal-appearing)Tj
7.472 0 Td
(medial)Tj
2.9424 0 Td
(tissue)Tj
2.4548 0 Td
(and)Tj
1.7291 0 Td
(to)Tj
-34.7525 -1.2982 Td
(include)Tj
3.1804 0 Td
(only)Tj
1.9786 0 Td
(injured)Tj
3.1407 0 Td
(areas)Tj
2.2451 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(intima)Tj
2.8573 0 Td
(and)Tj
1.7291 0 Td
(media)Tj
2.6929 0 Td
(layers.)Tj
2.7383 0 Td
(Data)Tj
2.1203 0 Td
(are)Tj
1.4513 0 Td
(expressed)Tj
4.0932 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4435 0 Td
(per-)Tj
-35.6369 -1.2983 Td
(centage)Tj
3.2371 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(total)Tj
2.0013 0 Td
(positively)Tj
4.0195 0 Td
(stained)Tj
3.0784 0 Td
(area)Tj
1.8822 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(aortic)Tj
2.5171 0 Td
(arch)Tj
1.9786 0 Td
(cross-sections.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 230.0031 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Real-time)Tj
4.4457 0 Td
(PCR)Tj
2.3102 0 Td
(\(RT-PCR\))Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 212.9952 cm
BT
/F6 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Total)Tj
2.313 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(extracted)Tj
3.8835 0 Td
(from)Tj
2.211 0 Td
(the)Tj
1.4683 0 Td
(aortic)Tj
2.5172 0 Td
(arch)Tj
1.9785 0 Td
(samples)Tj
3.3846 0 Td
(using)Tj
2.3867 0 Td
(1)Tj
0.6973 0 Td
(mL)Tj
1.5761 0 Td
(of)Tj
1.0148 0 Td
(TRIzol)Tj
2.9593 0 Td
(reagent,)Tj
3.4129 0 Td
(as)Tj
-33.766 -1.2982 Td
(described)Tj
4.0535 0 Td
(by)Tj
1.1792 0 Td
(the)Tj
1.4683 0 Td
(manufacturer)Tj
5.6863 0 Td
(\(Invitrogen,)Tj
5.0116 0 Td
(USA\).)Tj
2.6816 0 Td
(After)Tj
2.2847 0 Td
(incubation)Tj
4.5524 0 Td
(for)Tj
1.3833 0 Td
(5)Tj
0.6973 0 Td
(min)Tj
1.8538 0 Td
(at)Tj
0.9525 0 Td
(room)Tj
2.4264 0 Td
(temper-)Tj
-34.2309 -1.3039 Td
(ature,)Tj
2.5001 0 Td
(chloroform)Tj
4.7848 0 Td
(\(200)Tj
/F11 1 Tf
1.99 0 Td
[()]TJ
/F6 1 Tf
(L\))Tj
1.6044 0 Td
(was)Tj
1.7007 0 Td
(added)Tj
2.6589 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.4684 0 Td
(tubes)Tj
2.3357 0 Td
(and)Tj
1.7348 0 Td
(centrifuged)Tj
4.7849 0 Td
(at)Tj
0.9524 0 Td
(12,000)Tj
/F11 1 Tf
2.8233 0 Td
[()]TJ
/F6 1 Tf
0.788 0 Td
(g.)Tj
0.9071 0 Td
(The)Tj
1.7858 0 Td
(aqueous)Tj
-33.8454 -1.2983 Td
(phase)Tj
2.4831 0 Td
(was)Tj
1.7008 0 Td
(transferred)Tj
4.6374 0 Td
(to)Tj
1.0261 0 Td
(another)Tj
3.3279 0 Td
(tube,)Tj
2.1997 0 Td
(and)Tj
1.7348 0 Td
(then)Tj
2.0182 0 Td
(the)Tj
1.4684 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(pelleted)Tj
3.3221 0 Td
(by)Tj
1.1849 0 Td
(centrifugation)Tj
-29.0662 -1.2983 Td
(\(12,000)Tj
/F11 1 Tf
3.1634 0 Td
[()]TJ
/F6 1 Tf
0.788 0 Td
(g\))Tj
1.0262 0 Td
(with)Tj
1.9955 0 Td
(cold)Tj
1.9276 0 Td
(ethanol)Tj
3.2031 0 Td
(and)Tj
1.7291 0 Td
(dried)Tj
2.3414 0 Td
(in)Tj
1.0318 0 Td
(open)Tj
2.2167 0 Td
(air.)Tj
1.5194 0 Td
(RNA)Tj
2.262 0 Td
(pellets)Tj
2.7326 0 Td
(were)Tj
2.126 0 Td
(eluted)Tj
2.6645 0 Td
(in)Tj
1.0375 0 Td
(RNase-free)Tj
-31.7648 -1.2982 Td
(water)Tj
2.4321 0 Td
(and)Tj
1.7291 0 Td
(treated)Tj
2.9934 0 Td
(with)Tj
2.0012 0 Td
(DNase)Tj
2.9083 0 Td
(I)Tj
0.5613 0 Td
(\(Invitrogen,)Tj
5.0116 0 Td
(USA\).)Tj
2.6816 0 Td
(Samples)Tj
3.4979 0 Td
(of)Tj
1.0148 0 Td
(total)Tj
2.0012 0 Td
(RNA)Tj
2.2621 0 Td
(were)Tj
2.1203 0 Td
(quantified)Tj
4.3086 0 Td
(by)Tj
-35.5235 -1.3039 Td
(measuring)Tj
4.439 0 Td
(the)Tj
1.4683 0 Td
(optical)Tj
2.914 0 Td
(density)Tj
3.1011 0 Td
(at)Tj
0.9524 0 Td
(260)Tj
1.6441 0 Td
(and)Tj
1.7291 0 Td
(280)Tj
1.6498 0 Td
(nm)Tj
1.5874 0 Td
(\(Nano)Tj
2.7836 0 Td
(Vue)Tj
1.8708 0 Td
(Plus-spectrophotometer)Tj
9.8589 0 Td
(GE,)Tj
1.7291 0 Td
(NJ,)Tj
-35.7276 -1.2983 Td
(USA\).)Tj
2.6815 0 Td
(The)Tj
1.7802 0 Td
(integrity)Tj
3.6169 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(RNA)Tj
2.262 0 Td
(was)Tj
1.7008 0 Td
(assessed)Tj
3.4696 0 Td
(by)Tj
1.1848 0 Td
(the)Tj
1.4684 0 Td
(260/280)Tj
3.4015 0 Td
(nm)Tj
1.5874 0 Td
(ratio)Tj
2.0976 0 Td
(and)Tj
1.7292 0 Td
(by)Tj
1.1848 0 Td
(electrophoresis)Tj
-30.6479 -1.2983 Td
(using)Tj
2.3867 0 Td
(a)Tj
0.6576 0 Td
(1%)Tj
1.4514 0 Td
(agarose)Tj
3.2144 0 Td
(gel)Tj
1.355 0 Td
(stained)Tj
3.0784 0 Td
(with)Tj
2.0012 0 Td
(ethidium)Tj
3.8778 0 Td
(bromide.)Tj
-16.8263 -1.3039 Td
(The)Tj
1.7858 0 Td
(expression)Tj
4.4617 0 Td
(levels)Tj
2.3754 0 Td
(of)Tj
1.0148 0 Td
(AT1)Tj
1.9956 0 Td
(receptor)Tj
3.5546 0 Td
(\()Tj
/F12 1 Tf
[()37()-20()7()]TJ
/F6 1 Tf
(\),)Tj
3.3165 0 Td
(RAGE)Tj
2.8063 0 Td
(\()Tj
/F12 1 Tf
[()37()2()2()]TJ
/F6 1 Tf
(\))Tj
2.7212 0 Td
(and)Tj
1.7292 0 Td
(the)Tj
1.474 0 Td
(inflammatory)Tj
5.7032 0 Td
(media-)Tj
-34.1345 -1.2983 Td
(tors)Tj
1.7574 0 Td
(TNF-)Tj
/F10 1 Tf
()Tj
/F6 1 Tf
3.0161 0 Td
(\()Tj
/F12 1 Tf
[( )75(\n)11()]TJ
/F6 1 Tf
(\),)Tj
2.5058 0 Td
(interleukin)Tj
4.6431 0 Td
(\(IL\)-6)Tj
2.6022 0 Td
(\()Tj
/F12 1 Tf
[()6(\r)]TJ
/F6 1 Tf
(\),)Tj
2.1543 0 Td
(IL-10)Tj
2.4038 0 Td
(\()Tj
/F12 1 Tf
[()6()]TJ
/F6 1 Tf
(\),)Tj
2.6192 0 Td
(IL-1)Tj
/F10 1 Tf
()Tj
/F6 1 Tf
2.4094 0 Td
(\()Tj
/F12 1 Tf
[()6()]TJ
/F13 1 Tf
()Tj
/F6 1 Tf
(\),)Tj
2.6419 0 Td
(macrophage)Tj
5.1477 0 Td
(biomarker)Tj
ET
Q
q
1 j
1 J
0 w
576 737.1 m
36 737.1 l
36 737.6 l
576 737.6 l
f*
36 741.2598 107.1496 23.6976 re
W* n
q
107.0929 0 0 23.6409 36 741.3165 cm
q
/I0 Do
Q
Q
Q
q
0 0 612 792 re
W* n
1 j
1 J
0 w
7.9999 0 0 7.9999 422.2488 745.7952 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(Dietary)Tj
3.3307 0 Td
(sodium)Tj
3.3874 0 Td
(restrictio)Tj
3.7063 0 Td
(n)Tj
0.7654 0 Td
(and)Tj
1.8567 0 Td
(atherogenesis)Tj
ET
Q
q
1 j
1 J
0 w
36 48.0002 m
576 48.0002 l
576 47.5002 l
36 47.5002 l
f*
0.83 0.64 0.02 0 k
81.5811 34.9228 m
237.8835 34.9228 l
h
f*
0 g
7.9999 0 0 7.9999 36 36 cm
BT
/F0 1 Tf
1 TL
-0.005 Tc
0 0 Td
(PLOS)Tj
2.8559 0 Td
(ONE)Tj
2.3669 0 Td
(|)Tj
0.83 0.64 0.02 0 k
0.4748 0 Td
(https://doi.or)Tj
5.4142 0 Td
(g/10.137)Tj
3.8198 0 Td
(1/journal.po)Tj
5.1236 0 Td
(ne.01770)Tj
4.089 0 Td
(86)Tj
0 g
2.0906 0 Td
(May)Tj
2.0906 0 Td
(8,)Tj
1.0346 0 Td
(2017)Tj
35.7806 0 Td
(4)Tj
0.7654 0 Td
(/)Tj
ET
endstream
endobj
63 0 obj
<>stream
endstream
endobj
64 0 obj
<>stream
endstream
endobj
65 0 obj
<>stream
endstream
endobj
66 0 obj
<>stream
BT
66.402 0 Td
(22)Tj
ET
Q
endstream
endobj
67 0 obj
<>/XObject<>>>/CropBox[0 0 612 792]/MediaBox[0 0 612 792]/Parent 11 0 R/Annots 74 0 R/Contents 75 0 R/TrimBox[0 0 612 792]>>
endobj
73 0 obj
<>
endobj
76 0 obj
<>stream
/CIDInit/ProcSet findresource begin
12 dict begin begincmap
/CMapName/Adobe-Identity-UCS def
/CIDSystemInfo<>def
/CMapType 2 def
1 begincodespacerange
<0000>
endcodespacerange
4 beginbfchar
<0024><0041>
<0026><0043>
<002A><0047>
<0037><0054>
endbfchar
endcmap
CMapName currentdict /CMap defineresource pop end end
endstream
endobj
77 0 obj
<>/FontDescriptor 78 0 R/DW 600>>
endobj
78 0 obj
<>
endobj
79 0 obj
<>stream
cvt a &@ fpgm62 h glyf&e5