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Jingxin Li, Birong Yang, Manman Shi, Kai Yuan, Wei Guo, Mingshun Li, Gejiao Wang
Effects upon metabolic pathways and energy production by Sb(III) and As(III)/Sb(III)-oxidase gene aioA in Agrobacterium tumefaciens GW4
www.plosone.org
www.plosone.org
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([Sb\(III\)])Tj
-32.995 -1.3039 Td
(and)Tj
1.7291 0 Td
(incubated)Tj
4.1782 0 Td
(at)Tj
0.9525 0 Td
(28C)Tj
2.228 0 Td
(with)Tj
1.9956 0 Td
(100)Tj
1.6441 0 Td
(rpm)Tj
1.9332 0 Td
(shaking.)Tj
3.5433 0 Td
(The)Tj
1.7801 0 Td
(carbon)Tj
3.0104 0 Td
(source)Tj
2.8346 0 Td
(in)Tj
1.0375 0 Td
(the)Tj
1.4683 0 Td
(CDM)Tj
2.5115 0 Td
(medium)Tj
3.6055 0 Td
(is)Tj
-34.4519 -1.2982 Td
(sodium)Tj
3.2315 0 Td
(lactate.)Tj
2.999 0 Td
(When)Tj
2.6872 0 Td
(needed,)Tj
3.3222 0 Td
(50)Tj
/F14 1 Tf
1.1679 0 Td
[()]TJ
/F7 1 Tf
(M)Tj
1.6214 0 Td
(K)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 357.0519 679.748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 360.5669 681.3921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(H)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 368.2771 679.748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 371.8488 681.3921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Sb)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 381.6566 679.748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 385.2283 681.3921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(O)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 392.6551 679.748 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(7)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 398.4377 681.3921 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
([Sb\(V\)])Tj
3.2541 0 Td
(was)Tj
1.7008 0 Td
(added)Tj
2.6589 0 Td
(to)Tj
1.0261 0 Td
(the)Tj
1.474 0 Td
(CDM)Tj
2.5115 0 Td
(agar)Tj
1.9219 0 Td
(plate.)Tj
-34.3896 -1.2982 Td
(Sb\(III\))Tj
2.9026 0 Td
(oxidation)Tj
4.0422 0 Td
(was)Tj
1.6951 0 Td
(detected)Tj
3.566 0 Td
(using)Tj
2.3924 0 Td
(HPLC-HG-AFS)Tj
6.6216 0 Td
(\(Beijing)Tj
3.3675 0 Td
(Titan)Tj
2.3754 0 Td
(Instruments)Tj
5.1194 0 Td
(Co.,)Tj
1.8425 0 Td
(Ltd.,)Tj
-33.9247 -1.2983 Td
(China\))Tj
2.999 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
1.0261 0 Td
(Li,)Tj
1.2472 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(24)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 626.7968 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Analysis)Tj
3.8976 0 Td
(of)Tj
1.0441 0 Td
(ATP)Tj
2.1591 0 Td
(and)Tj
1.8614 0 Td
(NADH)Tj
3.0284 0 Td
(contents)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 609.7889 cm
BT
/F7 1 Tf
1 TL
0.007 Tc
0 0 Td
(To)Tj
1.3606 0 Td
(test)Tj
1.6441 0 Td
(the)Tj
1.5023 0 Td
(ATP)Tj
2.109 0 Td
(and)Tj
1.7631 0 Td
(NADH)Tj
3.1861 0 Td
(contents,)Tj
/F13 1 Tf
3.9515 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.1225 0 Td
[( )7()12(\r)6(\n)1()9()6()1()4(\n)2()]TJ
/F7 1 Tf
4.9776 0 Td
(strains)Tj
2.9367 0 Td
(were)Tj
2.1657 0 Td
(each)Tj
2.0749 0 Td
(inoculated)Tj
4.5354 0 Td
(into)Tj
1.8822 0 Td
(100)Tj
-35.2117 -1.2982 Td
(mL)Tj
1.5987 0 Td
(of)Tj
1.0375 0 Td
(CDM)Tj
2.5454 0 Td
(medium.)Tj
3.9062 0 Td
(When)Tj
2.7325 0 Td
(OD)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 333.2409 595.1622 cm
BT
/F7 1 Tf
1 TL
0.0093 Tc
0 0 Td
(600)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 346.4503 596.8062 cm
BT
/F7 1 Tf
1 TL
0.007 Tc
0 0 Td
(reached)Tj
3.4298 0 Td
(0.50.6,)Tj
3.4016 0 Td
(0)Tj
0.7087 0 Td
(or)Tj
1.1168 0 Td
(50)Tj
/F14 1 Tf
1.1962 0 Td
[()]TJ
/F7 1 Tf
(M)Tj
1.6384 0 Td
(Sb\(III\))Tj
2.9821 0 Td
(was)Tj
1.7291 0 Td
(added)Tj
2.7156 0 Td
(to)Tj
1.0488 0 Td
(each)Tj
-34.6107 -1.2982 Td
(culture.)Tj
3.3505 0 Td
(After)Tj
2.3414 0 Td
(incubation)Tj
4.6601 0 Td
(of)Tj
1.0318 0 Td
(2)Tj
0.7087 0 Td
(h,)Tj
0.9978 0 Td
(bacterial)Tj
3.7189 0 Td
(cells)Tj
1.9786 0 Td
(were)Tj
2.1656 0 Td
(harvested)Tj
4.1386 0 Td
(by)Tj
1.2075 0 Td
(centrifugation)Tj
6.0435 0 Td
(\(13,400)Tj
/F14 1 Tf
[()]TJ
/F7 1 Tf
(g,)Tj
-32.343 -1.3039 Td
(5)Tj
0.703 0 Td
(min,)Tj
2.1259 0 Td
(4C\))Tj
2.1373 0 Td
(and)Tj
1.7632 0 Td
(resuspended)Tj
5.3517 0 Td
(in)Tj
1.0602 0 Td
(1)Tj
0.703 0 Td
(mL)Tj
1.6043 0 Td
(of)Tj
1.0375 0 Td
(0.4)Tj
1.4286 0 Td
(M)Tj
1.1225 0 Td
(perchloric)Tj
4.405 0 Td
(acid)Tj
1.9219 0 Td
(with)Tj
2.0409 0 Td
(1.0)Tj
1.4287 0 Td
(mM)Tj
1.9502 0 Td
(EDTA.)Tj
3.1068 0 Td
(Then,)Tj
-33.8907 -1.2983 Td
(the)Tj
1.5023 0 Td
(suspension)Tj
4.7509 0 Td
(was)Tj
1.7347 0 Td
(sonicated)Tj
4.1103 0 Td
(on)Tj
1.2925 0 Td
(ice)Tj
1.3719 0 Td
(for)Tj
1.4173 0 Td
(5)Tj
0.703 0 Td
(min)Tj
1.8935 0 Td
(and)Tj
1.7632 0 Td
(centrifuged)Tj
4.9039 0 Td
(\(13,400)Tj
/F14 1 Tf
[()]TJ
/F7 1 Tf
(g,)Tj
4.5354 0 Td
(5)Tj
0.703 0 Td
(min,)Tj
2.1259 0 Td
(4C\))Tj
2.1374 0 Td
(to)Tj
-34.9452 -1.2983 Td
(remove)Tj
3.2825 0 Td
(the)Tj
1.5023 0 Td
(debris.)Tj
2.982 0 Td
(The)Tj
1.8142 0 Td
(pH)Tj
1.5307 0 Td
(of)Tj
1.0318 0 Td
(the)Tj
1.5024 0 Td
(supernatant)Tj
5.1079 0 Td
(was)Tj
1.7291 0 Td
(adjusted)Tj
3.668 0 Td
(to)Tj
1.0431 0 Td
(7.0)Tj
1.4344 0 Td
(with)Tj
2.0409 0 Td
(1.0)Tj
1.4286 0 Td
(M)Tj
1.1226 0 Td
(K)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 519.0803 543.1181 cm
BT
/F7 1 Tf
1 TL
0.0093 Tc
0 0 Td
(2)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 522.7086 544.8188 cm
BT
/F7 1 Tf
1 TL
0.007 Tc
0 0 Td
(CO)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 537.0519 543.1181 cm
BT
/F7 1 Tf
1 TL
0.0093 Tc
0 0 Td
(3)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 540.737 544.8188 cm
BT
/F7 1 Tf
1 TL
0.007 Tc
0 0 Td
(,)Tj
0.4535 0 Td
(and)Tj
1.7631 0 Td
(the)Tj
-36.2888 -1.3039 Td
(samples)Tj
3.4583 0 Td
(were)Tj
2.1656 0 Td
(subsequently)Tj
5.5389 0 Td
(filtered)Tj
3.1521 0 Td
(\(0.22)Tj
/F14 1 Tf
2.2677 0 Td
[()]TJ
/F7 1 Tf
(m)Tj
1.5704 0 Td
(filter\))Tj
2.5285 0 Td
(for)Tj
1.423 0 Td
(HPLC)Tj
2.7892 0 Td
(analysis)Tj
3.4186 0 Td
(\(HPLC)Tj
3.1408 0 Td
(2690)Tj
2.1656 0 Td
(series,)Tj
-33.6187 -1.2982 Td
(Waters,)Tj
3.3732 0 Td
(Massachusetts,)Tj
6.3042 0 Td
(USA\).)Tj
2.7383 0 Td
(At)Tj
1.2246 0 Td
(the)Tj
1.5023 0 Td
(same)Tj
2.3017 0 Td
(time,)Tj
2.3074 0 Td
(samples)Tj
3.4583 0 Td
(were)Tj
2.1656 0 Td
(taken)Tj
2.4775 0 Td
(for)Tj
1.423 0 Td
(detecting)Tj
3.9911 0 Td
(the)Tj
1.5023 0 Td
(pro-)Tj
-34.7695 -1.2983 Td
(tein)Tj
1.8028 0 Td
(concentration)Tj
5.9584 0 Td
(using)Tj
2.4435 0 Td
(Coomassie)Tj
4.6658 0 Td
(Brilliant)Tj
3.5773 0 Td
(Blue)Tj
2.0409 0 Td
(method)Tj
3.3902 0 Td
([)Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
2.1317 0 Td
(ATP)Tj
2.1089 0 Td
(and)Tj
1.7632 0 Td
(NADH)Tj
3.1861 0 Td
(in)Tj
1.06 0 Td
(each)Tj
-34.1288 -1.3039 Td
(sample)Tj
3.0841 0 Td
(were)Tj
2.1713 0 Td
(identified)Tj
4.1669 0 Td
(by)Tj
1.2019 0 Td
(comparing)Tj
4.6715 0 Td
(the)Tj
1.5023 0 Td
(retention)Tj
4.0082 0 Td
(times)Tj
2.4434 0 Td
(to)Tj
1.0489 0 Td
(standards,)Tj
4.4163 0 Td
(and)Tj
1.7631 0 Td
(the)Tj
1.5024 0 Td
(peak)Tj
2.1372 0 Td
(area)Tj
-34.1175 -1.2983 Td
(represented)Tj
5.0287 0 Td
(the)Tj
1.5023 0 Td
(content)Tj
3.3449 0 Td
(of)Tj
1.0374 0 Td
(each)Tj
2.075 0 Td
(energy)Tj
2.9707 0 Td
(substance.)Tj
4.439 0 Td
(The)Tj
1.8142 0 Td
(mobile)Tj
3.0557 0 Td
(phase)Tj
2.5398 0 Td
(contained)Tj
4.2973 0 Td
(90%)Tj
1.9616 0 Td
(50)Tj
1.1961 0 Td
(mM)Tj
-35.2627 -1.2982 Td
(phosphate)Tj
4.4334 0 Td
(buffer,)Tj
2.9253 0 Td
(10%)Tj
1.9616 0 Td
(acetonitrile)Tj
4.8585 0 Td
(and)Tj
1.7632 0 Td
(3.22)Tj
1.9162 0 Td
(g/L)Tj
1.5874 0 Td
(tetrabutylammonium)Tj
9.0198 0 Td
(bromide)Tj
3.7247 0 Td
(\(pH)Tj
1.8765 0 Td
(6.8\).)Tj
-34.0666 -1.2983 Td
(The)Tj
1.8142 0 Td
(flow)Tj
1.9899 0 Td
(velocity)Tj
3.3845 0 Td
(of)Tj
1.0318 0 Td
(the)Tj
1.5081 0 Td
(mobile)Tj
3.0557 0 Td
(phase)Tj
2.5342 0 Td
(was)Tj
1.7347 0 Td
(1)Tj
0.7087 0 Td
(mL/min.)Tj
3.8438 0 Td
(To)Tj
1.3606 0 Td
(eliminate)Tj
4.0478 0 Td
(error,)Tj
2.5512 0 Td
(each)Tj
2.0749 0 Td
(text)Tj
1.7518 0 Td
(was)Tj
1.7291 0 Td
(repli-)Tj
-35.121 -1.3039 Td
(cated)Tj
2.3811 0 Td
(three)Tj
2.3187 0 Td
(times.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 412.2141 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Mobility)Tj
3.5622 0 Td
(assay)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 395.2062 cm
BT
/F13 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0941 0 Td
[( )7()12(\r)6(\n)1()9()6()1()4(\n)2()]TJ
/F7 1 Tf
4.8586 0 Td
(strains)Tj
2.8629 0 Td
(were)Tj
2.1203 0 Td
(each)Tj
2.0296 0 Td
(inoculated)Tj
4.4221 0 Td
(into)Tj
1.8425 0 Td
(5)Tj
0.6973 0 Td
(mL)Tj
1.576 0 Td
(of)Tj
1.0148 0 Td
(CDM)Tj
2.5115 0 Td
(medium)Tj
3.6057 0 Td
(and)Tj
1.7348 0 Td
(incubated)Tj
4.1725 0 Td
(at)Tj
0.9525 0 Td
(28C)Tj
-35.4952 -1.2982 Td
(with)Tj
1.9955 0 Td
(120)Tj
1.6498 0 Td
(rpm)Tj
1.9275 0 Td
(shaking.)Tj
3.5433 0 Td
(When)Tj
2.6872 0 Td
(OD)Tj
ET
Q
q
1 j
1 J
0 w
7.4999 0 0 6.6659 332.844 380.5228 cm
BT
/F7 1 Tf
1 TL
-0.0053 Tc
0 0 Td
(600)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 345.7133 382.2236 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(reached)Tj
3.3561 0 Td
(0.10.2,)Tj
3.3109 0 Td
(the)Tj
1.474 0 Td
(strains)Tj
2.8573 0 Td
(were)Tj
2.126 0 Td
(each)Tj
2.0296 0 Td
(deposited)Tj
4.0705 0 Td
(at)Tj
0.9524 0 Td
(the)Tj
-34.7467 -1.3039 Td
(center)Tj
2.7099 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(soft)Tj
1.6724 0 Td
(agar)Tj
1.9276 0 Td
(plate)Tj
2.1373 0 Td
(\(CDM)Tj
2.8516 0 Td
(with)Tj
1.9955 0 Td
(0.25%)Tj
2.6305 0 Td
(agar\))Tj
2.2677 0 Td
(with)Tj
1.9956 0 Td
(the)Tj
1.4684 0 Td
(addition)Tj
3.5999 0 Td
(of)Tj
1.0148 0 Td
(0,)Tj
0.9184 0 Td
(10,)Tj
1.3947 0 Td
(25,)Tj
1.4003 0 Td
(50,)Tj
1.3946 0 Td
(200,)Tj
-33.8623 -1.2982 Td
(500)Tj
/F14 1 Tf
1.644 0 Td
[()]TJ
/F7 1 Tf
(M)Tj
1.6215 0 Td
(Sb\(III\))Tj
2.9026 0 Td
(or)Tj
1.0998 0 Td
(Sb\(V\).)Tj
2.8007 0 Td
(Then,)Tj
2.5568 0 Td
(the)Tj
1.4683 0 Td
(plates)Tj
2.5001 0 Td
(were)Tj
2.1259 0 Td
(incubated)Tj
4.1726 0 Td
(at)Tj
0.9525 0 Td
(28C)Tj
2.228 0 Td
(for)Tj
1.3889 0 Td
(72)Tj
1.1679 0 Td
(h,)Tj
0.9751 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(swim)Tj
2.3527 0 Td
(ring)Tj
-35.1549 -1.2983 Td
(diameters)Tj
4.1442 0 Td
(were)Tj
2.1203 0 Td
(measured)Tj
4.1102 0 Td
(to)Tj
1.0261 0 Td
(quantify)Tj
3.5432 0 Td
(the)Tj
1.474 0 Td
(extent)Tj
2.6759 0 Td
(of)Tj
1.0148 0 Td
(mobility)Tj
3.5887 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
(].)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 314.5889 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Microcalorimetric)Tj
7.6866 0 Td
(measurement)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 297.5811 cm
BT
/F7 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Metabolic)Tj
4.2179 0 Td
(activities)Tj
3.7133 0 Td
(of)Tj
/F13 1 Tf
1.0148 0 Td
[()]TJ
/F7 1 Tf
(.)Tj
/F13 1 Tf
1.0999 0 Td
[( )7()12(\r)6(\n)1()9()6()1()4(\n)2()]TJ
/F7 1 Tf
4.8585 0 Td
(strains)Tj
2.8573 0 Td
(were)Tj
2.126 0 Td
(evaluated)Tj
3.9912 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
0.652 0 Td
(third)Tj
2.211 0 Td
(generation)Tj
4.5071 0 Td
(thermal)Tj
-33.2445 -1.2982 Td
(activity)Tj
3.118 0 Td
(monitor)Tj
3.5377 0 Td
(\(TAMIII,)Tj
3.9911 0 Td
(Sweden\))Tj
3.6453 0 Td
([)Tj
0.83 0.64 0.02 0 k
(27)Tj
0 g
(].)Tj
2.075 0 Td
(Bacterial)Tj
3.702 0 Td
(cells)Tj
1.9162 0 Td
(were)Tj
2.126 0 Td
(each)Tj
2.0296 0 Td
(inoculated)Tj
4.422 0 Td
(into)Tj
1.8425 0 Td
(5)Tj
0.6917 0 Td
(mL)Tj
1.5817 0 Td
(of)Tj
-34.6788 -1.2983 Td
(CDM)Tj
2.5114 0 Td
(medium)Tj
3.6057 0 Td
(and)Tj
1.7291 0 Td
(incubated)Tj
4.1782 0 Td
(at)Tj
0.9525 0 Td
(28C)Tj
2.228 0 Td
(with)Tj
1.9956 0 Td
(120)Tj
1.6441 0 Td
(rpm)Tj
1.9332 0 Td
(shaking.)Tj
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