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Ivana Puglisi, Alessandro De Patrizio, Leonardo Schena, Thomas Jung, Maria Evoli, Antonella Pane, Nguyen Van Hoa, Mai Van Tri, Sandra Wright, Mauritz Ramstedt, Christer Olsson, Roberto Faedda, Gaetano Magnano di San Lio, Santa Olga Cacciola
Two previously unknown Phytophthora species associated with brown rot of Pomelo (Citrus grandis) fruits in Vietnam
www.plosone.org
www.plosone.org
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(medium\))Tj
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0.83 0.64 0.02 0 k
(7)Tj
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(].)Tj
1.6044 0 Td
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1.4741 0 Td
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3.9231 0 Td
(\(35)Tj
2.0296 0 Td
(mm\))Tj
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1.7292 0 Td
(plated)Tj
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(onto)Tj
2.0806 0 Td
(BNPRA-HMI)Tj
5.7543 0 Td
(agar)Tj
1.9219 0 Td
(in)Tj
1.0375 0 Td
(Petri)Tj
2.1373 0 Td
(dishes.)Tj
-32.8703 -1.2983 Td
(Petri)Tj
2.1316 0 Td
(dishes)Tj
2.6815 0 Td
(were)Tj
2.1147 0 Td
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4.1726 0 Td
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0.9467 0 Td
(24C)Tj
2.2224 0 Td
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1.3776 0 Td
(36)Tj
1.6838 0 Td
(days)Tj
1.9899 0 Td
(in)Tj
1.0318 0 Td
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1.4626 0 Td
(dark.)Tj
2.2677 0 Td
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4.904 0 Td
(of)Tj
/F14 1 Tf
1.0091 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
/F9 1 Tf
5.5672 0 Td
(colo-)Tj
-35.5632 -1.2982 Td
(nies)Tj
1.8198 0 Td
(emerging)Tj
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2.2053 0 Td
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1.1792 0 Td
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2.5285 0 Td
(hyphal)Tj
2.9253 0 Td
(tips)Tj
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2.6192 0 Td
(the)Tj
1.4683 0 Td
(ste-)Tj
-35.7843 -1.2983 Td
(reomicroscope)Tj
6.1398 0 Td
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2.0806 0 Td
(PDA)Tj
2.1996 0 Td
(and)Tj
1.7292 0 Td
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1.3946 0 Td
(vegetable)Tj
3.8948 0 Td
(juice)Tj
2.1203 0 Td
(agar)Tj
1.9219 0 Td
(\(V8A,)Tj
2.6418 0 Td
(Campbell)Tj
4.0762 0 Td
(Foods,)Tj
2.8743 0 Td
(Belgium\).)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 457.6818 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
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6.3591 0 Td
(and)Tj
1.8614 0 Td
(cultural)Tj
3.4016 0 Td
(characteristics)Tj
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Q
q
1 j
1 J
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/F9 1 Tf
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3.1464 0 Td
(types,)Tj
2.5001 0 Td
(growth)Tj
3.0728 0 Td
(rates)Tj
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0.9525 0 Td
(different)Tj
3.6566 0 Td
(temperatures)Tj
5.4879 0 Td
(and)Tj
1.7291 0 Td
(cardinal)Tj
3.4752 0 Td
(temperatures)Tj
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(for)Tj
1.389 0 Td
(growth,)Tj
-33.0064 -1.2982 Td
(and)Tj
1.7291 0 Td
(morphology)Tj
5.142 0 Td
(of)Tj
1.0148 0 Td
(sporangia,)Tj
4.354 0 Td
(chlamydospores)Tj
6.701 0 Td
(and)Tj
1.7292 0 Td
(hyphal)Tj
2.9253 0 Td
(swellings)Tj
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(were)Tj
2.1203 0 Td
(determined,)Tj
5.074 0 Td
(on)Tj
-34.6164 -1.2983 Td
(both)Tj
2.0579 0 Td
(V8A)Tj
2.0749 0 Td
(and)Tj
1.7292 0 Td
(PDA)Tj
2.1996 0 Td
(following)Tj
3.9798 0 Td
(published)Tj
4.1273 0 Td
(methods)Tj
3.685 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
1.2586 0 Td
(9)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
0.924 0 Td
(10)Tj
0 g
(].)Tj
1.7348 0 Td
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2.5796 0 Td
(90)Tj
1.1678 0 Td
(mm)Tj
1.8595 0 Td
(Petri)Tj
2.1373 0 Td
(dishes)Tj
2.6929 0 Td
(of)Tj
-34.2082 -1.2982 Td
(PDA)Tj
2.1996 0 Td
(and)Tj
1.7291 0 Td
(V8A)Tj
2.075 0 Td
(\(20)Tj
1.508 0 Td
(mL)Tj
1.5817 0 Td
(each\))Tj
2.3698 0 Td
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2.1203 0 Td
(inoculated)Tj
4.422 0 Td
(centrally)Tj
3.668 0 Td
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1.9956 0 Td
(mycelia)Tj
3.2882 0 Td
(agar)Tj
1.9275 0 Td
(plugs)Tj
2.3357 0 Td
(taken)Tj
2.4208 0 Td
(from)Tj
2.2054 0 Td
(an)Tj
-35.8467 -1.304 Td
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3.2201 0 Td
(growing)Tj
3.5319 0 Td
(colony)Tj
2.9027 0 Td
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1.9559 0 Td
(at)Tj
0.9524 0 Td
(24C.)Tj
2.4548 0 Td
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4.4957 0 Td
(dishes)Tj
2.6873 0 Td
(were)Tj
2.1203 0 Td
(incubated)Tj
4.1782 0 Td
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1.4683 0 Td
(dark)Tj
2.0523 0 Td
(between)Tj
-33.0574 -1.2982 Td
(4)Tj
0.6916 0 Td
(and)Tj
1.7348 0 Td
(40C)Tj
2.2223 0 Td
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2.0013 0 Td
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1.7518 0 Td
(intervals)Tj
3.6226 0 Td
(and)Tj
1.7292 0 Td
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1.0147 0 Td
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3.5377 0 Td
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3.0727 0 Td
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(by)Tj
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1.7064 0 Td
(orthogonal)Tj
4.6318 0 Td
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1.0148 0 Td
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4.269 0 Td
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2.7439 0 Td
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1.9219 0 Td
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(the)Tj
1.4683 0 Td
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(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
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-34.7752 -1.3039 Td
(dishes.)Tj
2.9139 0 Td
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1.7802 0 Td
(experiment)Tj
4.7792 0 Td
(was)Tj
1.7008 0 Td
(repeated)Tj
3.6339 0 Td
(twice.)Tj
2.5455 0 Td
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4.4617 0 Td
(hyphal)Tj
2.9311 0 Td
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(and)Tj
1.7292 0 Td
(chlamydospores)Tj
-30.2965 -1.2983 Td
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2.0296 0 Td
(two)Tj
1.7121 0 Td
(weeks)Tj
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(of)Tj
1.0148 0 Td
(growth)Tj
3.0727 0 Td
(at)Tj
0.9525 0 Td
(24C)Tj
2.228 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(two)Tj
1.7121 0 Td
(agar)Tj
1.9219 0 Td
(media)Tj
2.6929 0 Td
(used)Tj
2.0579 0 Td
(for)Tj
1.389 0 Td
(the)Tj
1.4683 0 Td
(growth)Tj
-33.8567 -1.2982 Td
(tests.)Tj
2.1883 0 Td
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2.6759 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4683 0 Td
(margin)Tj
3.1295 0 Td
(of)Tj
1.0148 0 Td
(actively)Tj
3.2201 0 Td
(growing)Tj
3.5263 0 Td
(cultures)Tj
3.4015 0 Td
(on)Tj
1.2756 0 Td
(V8A)Tj
2.075 0 Td
(were)Tj
2.1203 0 Td
(overlaid)Tj
3.4526 0 Td
(with)Tj
1.9955 0 Td
(non-ster-)Tj
-33.749 -1.304 Td
(ile)Tj
1.1565 0 Td
(soil)Tj
1.5987 0 Td
(filtrate,)Tj
3.0727 0 Td
(according)Tj
4.1896 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(method)Tj
3.3222 0 Td
(of)Tj
1.0148 0 Td
(Jung)Tj
2.0919 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1282 0 Td
([)Tj
0.83 0.64 0.02 0 k
(11)Tj
0 g
(],)Tj
2.075 0 Td
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(stimulate)Tj
3.8948 0 Td
(sporangium)Tj
5.0399 0 Td
(formation.)Tj
-33.046 -1.2982 Td
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5.0569 0 Td
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1.0148 0 Td
(50)Tj
1.1736 0 Td
(mature)Tj
3.0954 0 Td
(sporangia)Tj
4.1215 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
2.0296 0 Td
(isolate,)Tj
2.982 0 Td
(chosen)Tj
3.0104 0 Td
(at)Tj
0.9524 0 Td
(random,)Tj
3.6567 0 Td
(and)Tj
1.7291 0 Td
(their)Tj
2.109 0 Td
(characteristic)Tj
-31.9462 -1.2983 Td
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3.3448 0 Td
(were)Tj
2.126 0 Td
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4.8472 0 Td
(using)Tj
2.3924 0 Td
(a)Tj
0.652 0 Td
(light)Tj
2.0239 0 Td
(microscope)Tj
4.8416 0 Td
(at)Tj
/F15 1 Tf
0.9524 0 Td
[()]TJ
/F9 1 Tf
(400)Tj
2.2167 0 Td
(and)Tj
/F15 1 Tf
1.7291 0 Td
[()]TJ
/F9 1 Tf
(1000)Tj
2.6929 0 Td
(magnifications.)Tj
6.3722 0 Td
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-34.1912 -1.3039 Td
(microscopical)Tj
5.7883 0 Td
(examinations)Tj
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-23.0058 -1.2983 Td
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1.2755 0 Td
(carrot)Tj
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(\(CA\))Tj
2.2507 0 Td
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1.7291 0 Td
(clarified)Tj
3.4696 0 Td
(V8)Tj
1.389 0 Td
(juice)Tj
2.1203 0 Td
(agar)Tj
1.9218 0 Td
(\(cV8A\),)Tj
-33.6186 -1.2982 Td
(prepared)Tj
3.804 0 Td
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1.0148 0 Td
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4.0535 0 Td
(by)Tj
1.1792 0 Td
(Erwin)Tj
2.6646 0 Td
(and)Tj
1.7291 0 Td
(Ribeiro)Tj
3.1861 0 Td
([)Tj
0.83 0.64 0.02 0 k
(12)Tj
0 g
(],)Tj
2.075 0 Td
(and)Tj
1.7291 0 Td
(following)Tj
3.9798 0 Td
(the)Tj
1.4684 0 Td
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3.3221 0 Td
(reported)Tj
3.6624 0 Td
(by)Tj
1.1849 0 Td
(Bra-)Tj
-35.053 -1.2983 Td
(sier)Tj
1.644 0 Td
(et)Tj
0.9411 0 Td
(al.)Tj
1.1225 0 Td
([)Tj
0.83 0.64 0.02 0 k
(8)Tj
0 g
(].)Tj
1.6044 0 Td
(A1)Tj
1.3777 0 Td
(and)Tj
1.7291 0 Td
(A2)Tj
1.3833 0 Td
(tester)Tj
2.3924 0 Td
(isolates)Tj
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(sourced)Tj
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2.2054 0 Td
(the)Tj
1.4683 0 Td
(Di3A)Tj
2.3811 0 Td
(culture)Tj
3.0387 0 Td
(collection)Tj
4.1102 0 Td
(and)Tj
1.7292 0 Td
(they)Tj
-35.7333 -1.3039 Td
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2.1203 0 Td
(Pf2b)Tj
2.0522 0 Td
(and)Tj
1.7291 0 Td
(CH230)Tj
3.0785 0 Td
(for)Tj
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1.3832 0 Td
[()]TJ
/F9 1 Tf
(.)Tj
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0.9978 0 Td
[( )18()4()7()12()7()4()16( )6()6()]TJ
/F9 1 Tf
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4.5298 0 Td
(isolated)Tj
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2.2054 0 Td
(pomelo)Tj
3.2371 0 Td
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1.0375 0 Td
(Vietnam)Tj
3.7077 0 Td
([)Tj
0.83 0.64 0.02 0 k
(13)Tj
0 g
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2.0749 0 Td
(and)Tj
1.7292 0 Td
(AX1aC)Tj
-33.1708 -1.2983 Td
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1.7291 0 Td
(CC1aL)Tj
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1.389 0 Td
[()]TJ
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(.)Tj
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0.9921 0 Td
[()1()14()7()20()11(\r)10()22()10()6()11()20()]TJ
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5.3065 0 Td
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(the)Tj
1.4683 0 Td
(rhizosphere)Tj
4.9322 0 Td
(soil)Tj
1.5988 0 Td
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1.0148 0 Td
[()13()14()7()-9()-4()]TJ
2.6248 0 Td
[()16()12()20()16( )22()7()12()12()]TJ
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4.4788 0 Td
(trees)Tj
2.0976 0 Td
(in)Tj
-36.1188 -1.2982 Td
(Italy)Tj
1.9899 0 Td
([)Tj
0.83 0.64 0.02 0 k
(14)Tj
0 g
(].)Tj
-0.7937 -1.304 Td
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1.627 0 Td
(both)Tj
2.0636 0 Td
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1.8766 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
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5.5729 0 Td
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1.1735 0 Td
(h)Tj
0.7484 0 Td
(at)Tj
0.9524 0 Td
(20C)Tj
-33.4202 -1.2983 Td
(under)Tj
2.6191 0 Td
(continuous)Tj
4.7339 0 Td
(daylight.)Tj
3.668 0 Td
(The)Tj
1.7801 0 Td
(Petri)Tj
2.143 0 Td
(solution)Tj
3.4809 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0182 0 Td
(replaced)Tj
3.583 0 Td
(with)Tj
2.0013 0 Td
(sterile)Tj
2.6078 0 Td
(water)Tj
2.4321 0 Td
(and)Tj
1.7292 0 Td
(plates)Tj
-34.4974 -1.2983 Td
(incubated)Tj
4.1782 0 Td
(for)Tj
1.3833 0 Td
(a)Tj
0.6519 0 Td
(further)Tj
3.0331 0 Td
(4872)Tj
2.6362 0 Td
(h.)Tj
0.9751 0 Td
(Once)Tj
2.3641 0 Td
(sporangia)Tj
4.1215 0 Td
(were)Tj
2.126 0 Td
(plentiful)Tj
3.583 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(discs)Tj
2.16 0 Td
(of)Tj
1.0148 0 Td
(mycelia,)Tj
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(the)Tj
-34.486 -1.3039 Td
(plates)Tj
2.5001 0 Td
(were)Tj
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(kept)Tj
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(at)Tj
0.9524 0 Td
(4C)Tj
1.7518 0 Td
(for)Tj
1.389 0 Td
(1)Tj
0.6916 0 Td
(h)Tj
0.7484 0 Td
(and)Tj
1.7291 0 Td
(returned)Tj
3.7134 0 Td
(to)Tj
1.0261 0 Td
(room)Tj
2.4264 0 Td
(temperature)Tj
5.125 0 Td
(to)Tj
1.0262 0 Td
(induce)Tj
2.9366 0 Td
(zoospore)Tj
3.8268 0 Td
(release.)Tj
-33.9191 -1.2982 Td
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4.0138 0 Td
(concentration)Tj
5.8166 0 Td
(was)Tj
1.7008 0 Td
(determined)Tj
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(using)Tj
2.3867 0 Td
(a)Tj
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(haemocytometer)Tj
6.9505 0 Td
(and)Tj
1.7291 0 Td
(its)Tj
1.1452 0 Td
(concentration)Tj
5.8223 0 Td
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-35.0756 -1.2983 Td
(adjusted)Tj
3.5772 0 Td
(to)Tj
1.0262 0 Td
(104)Tj
1.6441 0 Td
(zoospores)Tj
4.1895 0 Td
(ml-1.)Tj
2.3358 0 Td
(A)Tj
0.9014 0 Td
(drop)Tj
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(\(200)Tj
/F15 1 Tf
1.9843 0 Td
[()]TJ
/F9 1 Tf
(l\))Tj
1.3152 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4684 0 Td
(suspension)Tj
4.6431 0 Td
(was)Tj
1.6951 0 Td
(pipetted)Tj
3.4979 0 Td
(onto)Tj
2.0806 0 Td
(the)Tj
1.4684 0 Td
(non-)Tj
-34.9906 -1.3039 Td
(wounded)Tj
3.9627 0 Td
(rind)Tj
1.9389 0 Td
(of)Tj
1.0148 0 Td
(fruits)Tj
2.3301 0 Td
(\(5)Tj
1.0375 0 Td
(fruits)Tj
2.3357 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
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(citrus)Tj
2.4718 0 Td
(variety)Tj
2.9254 0 Td
(per)Tj
1.5307 0 Td
(isolate\))Tj
3.0954 0 Td
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1.0374 0 Td
(a)Tj
0.652 0 Td
(polar)Tj
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(near)Tj
2.0013 0 Td
(the)Tj
-35.1607 -1.2983 Td
(rosette.)Tj
3.1294 0 Td
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(distilled)Tj
3.3846 0 Td
(water)Tj
2.4321 0 Td
(was)Tj
1.7007 0 Td
(included)Tj
3.7134 0 Td
(as)Tj
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(a)Tj
0.652 0 Td
(control.)Tj
3.3505 0 Td
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2.5682 0 Td
(were)Tj
2.1259 0 Td
(incubated)Tj
4.1783 0 Td
(in)Tj
1.0318 0 Td
(plastic)Tj
2.7666 0 Td
(bags)Tj
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(at)Tj
-36.7594 -1.2983 Td
(2426C)Tj
3.6963 0 Td
(in)Tj
1.0318 0 Td
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3.5943 0 Td
(water)Tj
2.4321 0 Td
(was)Tj
1.7008 0 Td
(nebulized)Tj
4.1045 0 Td
(into)Tj
1.8369 0 Td
(the)Tj
1.474 0 Td
(bags)Tj
1.9842 0 Td
(to)Tj
1.0261 0 Td
(reach)Tj
2.3981 0 Td
(a)Tj
0.6577 0 Td
(high)Tj
2.0069 0 Td
(relative)Tj
-31.9122 -1.3039 Td
(humidity)Tj
3.9117 0 Td
(and)Tj
1.7291 0 Td
(prevent)Tj
3.2542 0 Td
(dehydration)Tj
5.108 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(inoculum.)Tj
4.3143 0 Td
(In)Tj
1.1112 0 Td
(all)Tj
1.1452 0 Td
(three)Tj
2.262 0 Td
(experiments)Tj
5.142 0 Td
(the)Tj
1.4684 0 Td
(size)Tj
1.6838 0 Td
(of)Tj
1.0148 0 Td
(each)Tj
-34.6278 -1.2982 Td
(lesion)Tj
2.5738 0 Td
(induced)Tj
3.4639 0 Td
(by)Tj
1.1792 0 Td
(artificial)Tj
3.5206 0 Td
(inoculation)Tj
4.7962 0 Td
(was)Tj
1.6951 0 Td
(determined)Tj
4.8529 0 Td
(as)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(mean)Tj
2.4491 0 Td
(of)Tj
1.0148 0 Td
(two)Tj
1.7065 0 Td
(orthogonal)Tj
4.6318 0 Td
(diame-)Tj
-34.367 -1.2983 Td
(ters,)Tj
1.8991 0 Td
(six)Tj
1.3096 0 Td
(days)Tj
2.0013 0 Td
(after)Tj
2.0352 0 Td
(the)Tj
1.4684 0 Td
(inoculation.)Tj
-7.5174 -1.3039 Td
(The)Tj
1.7858 0 Td
(five)Tj
/F14 1 Tf
1.6497 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
/F9 1 Tf
5.5729 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.126 0 Td
(also)Tj
1.7688 0 Td
(used)Tj
2.0579 0 Td
(to)Tj
1.0261 0 Td
(inoculate)Tj
3.8948 0 Td
(2-year-old)Tj
4.3597 0 Td
(potted)Tj
2.7949 0 Td
(trees)Tj
2.0976 0 Td
(of)Tj
1.0148 0 Td
(pomelo)Tj
-34.4633 -1.2983 Td
(`Chandler')Tj
4.3993 0 Td
(and)Tj
1.7348 0 Td
(sweet)Tj
2.4094 0 Td
(orange)Tj
2.9707 0 Td
(`Lane)Tj
2.3868 0 Td
(Late')Tj
2.1316 0 Td
(grafted)Tj
3.0274 0 Td
(on)Tj
1.2756 0 Td
(`Carrizo')Tj
3.685 0 Td
(citrange)Tj
3.4525 0 Td
(\()Tj
/F14 1 Tf
[()]TJ
/F9 1 Tf
(.)Tj
/F14 1 Tf
1.406 0 Td
[()10()12( )6()2( )10()-4()]TJ
/F9 1 Tf
(`)Tj
3.4186 0 Td
(Washington)Tj
-32.2977 -1.2982 Td
(Navel')Tj
2.7268 0 Td
(x)Tj
/F14 1 Tf
0.686 0 Td
[()23()11( )6()1()12()-9()-4()]TJ
3.617 0 Td
[()7()-9()12()9()10()12()4()22()11()]TJ
/F9 1 Tf
(\))Tj
3.9968 0 Td
(rootstock,)Tj
4.2123 0 Td
(which)Tj
2.6475 0 Td
(is)Tj
0.8504 0 Td
(known)Tj
3.0047 0 Td
(a)Tj
/F14 1 Tf
0.652 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
/F9 1 Tf
(-resistant)Tj
9.2862 0 Td
(rootstock)Tj
3.9912 0 Td
([)Tj
0.83 0.64 0.02 0 k
(3)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
-35.6709 -1.2983 Td
(4)Tj
0 g
(,)Tj
0.83 0.64 0.02 0 k
0.9185 0 Td
(23)Tj
0 g
(].)Tj
1.7403 0 Td
(Trees)Tj
2.4095 0 Td
(\(5)Tj
1.0318 0 Td
(trees)Tj
2.0976 0 Td
(per)Tj
1.5363 0 Td
(treatment\))Tj
4.4731 0 Td
(were)Tj
2.126 0 Td
(inoculated)Tj
4.422 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(twigs)Tj
2.2961 0 Td
(\(3)Tj
1.0374 0 Td
(twigs,)Tj
2.5172 0 Td
(1.52)Tj
2.3924 0 Td
(cm)Tj
1.4627 0 Td
(in)Tj
1.0374 0 Td
(diame-)Tj
-34.2422 -1.3039 Td
(ter,)Tj
1.5363 0 Td
(per)Tj
1.5307 0 Td
(tree\))Tj
2.0806 0 Td
(by)Tj
1.1792 0 Td
(cutting)Tj
3.0501 0 Td
(bark)Tj
2.0296 0 Td
(disks)Tj
2.2337 0 Td
(with)Tj
1.9955 0 Td
(a)Tj
0.652 0 Td
(cork-borer)Tj
4.5524 0 Td
(\(diameter)Tj
4.1216 0 Td
(3)Tj
0.6973 0 Td
(mm\))Tj
2.1997 0 Td
(and)Tj
1.7291 0 Td
(inserting)Tj
3.77 0 Td
(an)Tj
1.2019 0 Td
(agar)Tj
-34.5597 -1.2983 Td
(plug)Tj
1.9728 0 Td
(from)Tj
2.211 0 Td
(actively)Tj
3.2202 0 Td
(growing)Tj
3.5263 0 Td
(cultures,)Tj
3.6283 0 Td
(with)Tj
1.9956 0 Td
(mycelium)Tj
4.2009 0 Td
(inside)Tj
2.6135 0 Td
(facing)Tj
2.6476 0 Td
(the)Tj
1.4683 0 Td
(wood.)Tj
2.6759 0 Td
(Trees)Tj
2.4094 0 Td
(were)Tj
2.1203 0 Td
(inocu-)Tj
-34.6901 -1.2983 Td
(lated)Tj
2.1486 0 Td
(also)Tj
1.7688 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(basal)Tj
2.1997 0 Td
(portion)Tj
3.2371 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.474 0 Td
(stem,)Tj
2.3471 0 Td
(on)Tj
1.2756 0 Td
(the)Tj
1.4683 0 Td
(rootstock,)Tj
4.2123 0 Td
(at)Tj
0.9524 0 Td
(two)Tj
1.7121 0 Td
(heights)Tj
3.0898 0 Td
(from)Tj
2.2053 0 Td
(the)Tj
1.4684 0 Td
(ground)Tj
3.1691 0 Td
(\(5)Tj
-36.4873 -1.3039 Td
(and)Tj
1.7291 0 Td
(15)Tj
1.1735 0 Td
(cm\).)Tj
2.0239 0 Td
(Trees)Tj
2.4151 0 Td
(inoculated)Tj
4.422 0 Td
(with)Tj
1.9956 0 Td
(plain)Tj
2.2337 0 Td
(agar)Tj
1.9276 0 Td
(blocks)Tj
2.7609 0 Td
(were)Tj
2.1203 0 Td
(included)Tj
3.7133 0 Td
(as)Tj
1.0148 0 Td
(controls.)Tj
3.7077 0 Td
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1.7802 0 Td
(removed)Tj
-33.0177 -1.2982 Td
(bark)Tj
2.0295 0 Td
(disk)Tj
1.8709 0 Td
(was)Tj
1.7008 0 Td
(replaced,)Tj
3.8097 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4684 0 Td
(wound)Tj
3.016 0 Td
(was)Tj
1.7008 0 Td
(then)Tj
2.0182 0 Td
(sealed)Tj
2.6362 0 Td
(with)Tj
1.9956 0 Td
(Parafilm)Tj
/F16 1 Tf
(1)Tj
/F9 1 Tf
(.)Tj
4.8642 0 Td
(Trees)Tj
2.4095 0 Td
(were)Tj
2.1203 0 Td
(grown)Tj
2.8006 0 Td
(in)Tj
-36.1698 -1.2983 Td
(a)Tj
0.652 0 Td
(greenhouse)Tj
4.8018 0 Td
(at)Tj
0.9524 0 Td
(a)Tj
0.652 0 Td
(temperature)Tj
5.1306 0 Td
(ranging)Tj
3.3166 0 Td
(from)Tj
2.2053 0 Td
(22)Tj
1.1735 0 Td
(to)Tj
1.0262 0 Td
(32C.)Tj
2.4491 0 Td
(After)Tj
2.2847 0 Td
(inoculation,)Tj
5.023 0 Td
(twigs)Tj
2.296 0 Td
(and)Tj
1.7291 0 Td
(stems)Tj
-33.6923 -1.3039 Td
(were)Tj
2.1202 0 Td
(inspected)Tj
4.0139 0 Td
(daily)Tj
2.1486 0 Td
(for)Tj
1.3833 0 Td
(symptoms.)Tj
4.5864 0 Td
(Thirty)Tj
2.7496 0 Td
(days)Tj
1.9956 0 Td
(after)Tj
2.0353 0 Td
(inoculation,)Tj
5.0229 0 Td
(the)Tj
1.4684 0 Td
(bark)Tj
2.0296 0 Td
(around)Tj
3.1351 0 Td
(the)Tj
1.4683 0 Td
(inocula-)Tj
-34.1572 -1.2983 Td
(tion)Tj
1.8424 0 Td
(points)Tj
2.7156 0 Td
(of)Tj
1.0148 0 Td
(both)Tj
2.058 0 Td
(twigs)Tj
2.296 0 Td
(and)Tj
1.7348 0 Td
(stem)Tj
2.1203 0 Td
(was)Tj
1.7008 0 Td
(removed)Tj
3.7474 0 Td
(and)Tj
1.7291 0 Td
(the)Tj
1.4683 0 Td
(length)Tj
2.7326 0 Td
(of)Tj
1.0148 0 Td
(discoloration)Tj
5.4822 0 Td
(on)Tj
1.2755 0 Td
(the)Tj
1.4684 0 Td
(wood)Tj
-34.401 -1.2983 Td
(was)Tj
1.695 0 Td
(measured.)Tj
4.337 0 Td
(The)Tj
1.7802 0 Td
(measurement)Tj
5.6749 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(lesion)Tj
2.5739 0 Td
(included)Tj
3.7077 0 Td
(the)Tj
1.474 0 Td
(diameter)Tj
3.7814 0 Td
(\(5mm\))Tj
3.016 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
1.4683 0 Td
(hole)Tj
1.9276 0 Td
(made)Tj
-34.9339 -1.2982 Td
(with)Tj
1.9955 0 Td
(the)Tj
1.4684 0 Td
(cork)Tj
2.0182 0 Td
(borer.)Tj
2.6249 0 Td
(The)Tj
1.7858 0 Td
(test)Tj
1.5987 0 Td
(was)Tj
1.7008 0 Td
(repeated)Tj
3.6397 0 Td
(twice.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 330.1795 cm
BT
/F0 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Statistical)Tj
4.4504 0 Td
(analyses)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 313.2283 cm
BT
/F9 1 Tf
1 TL
-0.004 Tc
0 0 Td
(Pathogenicity)Tj
5.6919 0 Td
(data)Tj
1.9162 0 Td
(were)Tj
2.1203 0 Td
(analyzed)Tj
3.702 0 Td
(by)Tj
1.1849 0 Td
(one-way)Tj
3.617 0 Td
(analysis)Tj
3.3278 0 Td
(of)Tj
1.0148 0 Td
(variance)Tj
3.5773 0 Td
(\(ANOVA\))Tj
4.4447 0 Td
(using)Tj
2.3924 0 Td
(Tukey's)Tj
-32.9893 -1.3039 Td
(HSD)Tj
2.1996 0 Td
(test)Tj
1.5988 0 Td
(\(Honestly)Tj
4.1669 0 Td
(Significant)Tj
4.5013 0 Td
(Difference\))Tj
4.7565 0 Td
(as)Tj
1.0148 0 Td
(a)Tj
0.652 0 Td
(post-hoc)Tj
3.7134 0 Td
(test)Tj
1.5987 0 Td
(\(XLSTAT)Tj
4.1386 0 Td
(2008)Tj
2.1203 0 Td
(software\).)Tj
4.1499 0 Td
(Differ-)Tj
-34.6108 -1.2982 Td
(ences)Tj
2.398 0 Td
(at)Tj
0.9525 0 Td
(P)Tj
/F17 1 Tf
0 Tc
0.7823 0 Td
()Tj
/F9 1 Tf
-0.004 Tc
0.9865 0 Td
(0.05)Tj
1.8765 0 Td
(were)Tj
2.1203 0 Td
(considered)Tj
4.6034 0 Td
(significant.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 261.0141 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.5023 Td
(Morphological)Tj
6.3591 0 Td
(identification)Tj
5.6976 0 Td
(of)Tj
/F8 1 Tf
1.0489 0 Td
(Phytophthora)Tj
/F0 1 Tf
6.0992 0 Td
(isolates)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F14 1 Tf
1 TL
-0.004 Tc
0 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
/F9 1 Tf
5.5728 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.1203 0 Td
(consistently)Tj
4.9606 0 Td
(obtained)Tj
3.7304 0 Td
(from)Tj
2.2053 0 Td
(symptomatic)Tj
5.4198 0 Td
(pomelo)Tj
3.2429 0 Td
(fruits)Tj
2.3357 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.8198 0 Td
(1)Tj
0 g
(\))Tj
-34.5257 -1.2982 Td
(sampled)Tj
3.5489 0 Td
(in)Tj
1.0375 0 Td
(citrus)Tj
2.4661 0 Td
(groves)Tj
2.8006 0 Td
(at)Tj
0.9525 0 Td
(Tam)Tj
2.0862 0 Td
(Bin.)Tj
1.8425 0 Td
(A)Tj
0.9015 0 Td
(total)Tj
2.0069 0 Td
(of)Tj
1.0148 0 Td
(89)Tj
/F14 1 Tf
1.1678 0 Td
[()4()24()-15()11()11(\r)10()22()10()6()11()20()]TJ
/F9 1 Tf
5.5729 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.126 0 Td
(characterized)Tj
-30.6423 -1.2983 Td
(in)Tj
1.0318 0 Td
(this)Tj
1.6781 0 Td
(study:)Tj
2.6135 0 Td
(38)Tj
1.1735 0 Td
(isolates)Tj
3.1181 0 Td
(were)Tj
2.1203 0 Td
(obtained)Tj
3.7304 0 Td
(from)Tj
2.211 0 Td
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