Gene expression of distinct ectoenzymes, transporters and channels in the T cell subsets isolated from lung tissue under basal conditions and 7real-time PCR.
(A) Under basal conditions, T cell subsets expressed various ectoenzymes of ATP and NAD degradation cascade as well as nucleotide and nucleoside transporters and channels. Gene expression was normalized to beta-actin and relative expression levels are depicted. (B) In the diseased state, Cd38, Cd39 and Cd73were significantly upregulated on T helper cells and tended to be increased in cytotoxic and regulatory T cells. Ent1 transcripts were significantly increased in T helper cells. Data are mean ± SD (n = 4 mice per group). Fold changes were calculated by comparing the expression under basal conditions to that in the injured lung 7 d post induction of ALI and statistical significance was then assessed by Mann-Whitney U test. *P<0.05, **P<0.01, ***P<0.0001. ALI = acute lung injury, Art2.b = ADP-ribosyltransferase 2b, ATP = adenosine triphosphate, AU = arbitrary units, Cnt = concentrative nucleoside transporter, Cx = connexin, Ent = equilibrative nucleoside transporter, LPS = lipopolysacch, NAD = nicotinamide adenine dinucleotide, Panx-1 = pannexin-1, SD = standard deviation.