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Influence of citrate concentration on the activation of blood cells in an in vitro dialysis setup

Fig 1

Scheme of the experimental set-up and levels of ionized calcium and citrate over the course of the experiment.

(a) Freshly drawn whole blood was anticoagulated with either 3 mM or 4 mM citrate supplemented with 3 IU/l unfractionated heparin and recirculated as described in the Methods section. Samples were taken at the indicated time points after the dialyzer (s) and analyzed for biocompatibility parameters as shown in Fig 2; (b) levels of ionized calcium, and (c) citrate levels over the course of the experiment.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0199204.g001