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Expression and purification of the mammalian translocator protein for structural studies

Fig 5

Optimisation of the TSPO-YFP fusion constructs.

(A) Construct design for TSPO-YFP fusions. The linker between the proteins was shortened with two amino acid steps. (B): Sequence alignment of linker region for all five fusion constructs. (C) Expression level of the best clone of the TSPO-YFP fusion constructs after stable cell line generation analysed by FSEC.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0198832.g005