TRESK background potassium channel is not gated at the helix bundle crossing near the cytoplasmic end of the pore
Mutant mouse TRESK channels were expressed in HEK293T cells. Experiments were done on excised inside-out patches. TRESK currents were measured at +60 mV by switching the K+-free bath solution to a high K+ solution. Barium (1 mM) was applied at a holding potential of -80 mV and block was initiated by depolarizing the membrane to +60 mV. A and B, The current recordings of the Ba2+ block recorded for the different mutants were fitted with a double exponential equation. The time constants of the fitted equations are illustrated as a scatter plot. The average values are plotted as columns. The differences between the groups were not statistically significant (ANOVA).