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TRESK background potassium channel is not gated at the helix bundle crossing near the cytoplasmic end of the pore

Fig 5

The rate of Ba2+ inhibition is similar in TRESK mutants mimicking the phosphorylated and dephosphorylated channel.

Mutant mouse TRESK channels were expressed in HEK293T cells. Experiments were done on excised inside-out patches. TRESK currents were measured at +60 mV by switching the K+-free bath solution to a high K+ solution. Barium (1 mM) was applied at a holding potential of -80 mV and block was initiated by depolarizing the membrane to +60 mV. A and B, The current recordings of the Ba2+ block recorded for the different mutants were fitted with a double exponential equation. The time constants of the fitted equations are illustrated as a scatter plot. The average values are plotted as columns. The differences between the groups were not statistically significant (ANOVA).

Fig 5

doi: https://doi.org/10.1371/journal.pone.0197622.g005