Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on Matrigel-coated plates at 9000 cells/cm2 and grown for 4 days. Cells were treated with PUFAs (LA, AA, ALA, EPA and DHA) individually at 125 μM for 8 h. Protein levels of MCM2, p-cyclinD1, cyclin D1 and VE-cadherin were determined by Western blotting of cell lysates prepared after treatments. Representative blots are shown in Panel A. Densitometry was used to quantify the intensity of the bands in the upper panel and data were normalized to a band visualized by Ponceau staining. Data are presented as means ± SEM (n = 3) for MCM2 (Panel B), p-cyclin D1/cyclin D1 (Panel C) and VE-cadherin (Panel D). *Significantly different (p <0.05) from vehicle control.