Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on Matrigel-coated plates at 9000 cells/cm2 and grown for 4 days. Cells were treated with PUFAs (LA, AA, ALA, EPA and DHA) individually at 125 μM for 8 h. Protein levels of cleaved caspase-3 and caspase-3 were determined by Western blotting of cell lysates prepared after treatments. Representative blots are shown in Panel A (subconfluent) and Panel B (confluent). Densitometry was used to quantify the intensity of the bands in the upper panels. Data are presented as means ± SEM (n = 3) for cleaved caspase-3/caspase-3 (Panel C). *Significantly different (p <0.05) from vehicle control.