Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on plastic culture plates at 9000 cells/cm2 in the absence (Panel A-E) or presence of Matrigel (Panel A’-E’). Growing cells (day 4) or confluent cells (day 8) were treated with different PUFAs [LA (Panel A, A’), AA (Panel B, B’), ALA (Panel C, C’), EPA (Panel D, D’) and DHA (Panel E, E’)] at final concentrations of 1, 5, 20, 40, 60, 80, 100, 125 and 150 μM for 24 hours, at which point, DNA synthesis was assessed using the BrdU cell proliferation assay. Data are normalized with vehicle control values and plotted as means ± SEM (n = 3). *Significantly different (p <0.05) from the vehicle control for the respective growth state.