Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on plastic culture plates at 9000 cells/cm2 in the absence (Panel A-E) or presence of Matrigel (Panel A’-E’). Growing cells (day 4) or confluent cells (day 8) were treated with different PUFAs [LA (Panel A, A’), AA (Panel B, B’), ALA (Panel C, C’), EPA (Panel D, D’) and DHA (Panel E, E’)] at final concentrations of 1, 5, 20, 40, 60, 80, 100, 125 and 150 μM for 24 hours, at which point, cell viability was assessed using the CCK-8 assay. Data are normalized with vehicle control values and plotted as means ± SEM (n = 3). *Significantly different (p <0.05) from the vehicle control for the respective growth state.