Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on plastic plates at 9000 cells/cm2 and grown for 12 days. Protein levels of cleaved caspase-3 and caspase-3 were determined by Western blotting of cell lysates prepared each day from day 4 to day 12. Representative blots are shown in Panel A (Matrigel) and B (Plastic). Densitometry was used to quantify the intensity of the bands in Panel A and B, and data were normalized to a band visualized by Ponceau staining (Panel C). Data are presented as means ± SEM (n = 3).