Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded on plastic plates at 9000 cells/cm2 and grown for 12 days. Protein levels of MCM2, p-cyclinD1, cyclin D1 and VE-cadherin were determined by Western blotting of cell lysates prepared each day from day 4 to day 12. Representative blots are shown in Panel A. Densitometry was used to quantify the intensity of the bands in the upper panel and data were normalized to a band visualized by Ponceau staining. Data are presented as means ± SEM (n = 3) for MCM2 (Panel B), p-cyclin D1/cyclin D1 (Panel C) and VE-cadherin (Panel D).