Importance of extracellular matrix and growth state for the EA.hy926 endothelial cell response to polyunsaturated fatty acids
EA.hy926 endothelial cells were seeded at 9000 cells/cm2 on 96 well plates pre-coated with Matrigel (5 μL Matrigel mixed in 95 μL DMEM) or without Matrigel (Panel A) or Collagen Type I dissolved in acetic acid at concentrations of 6 and 10 μg/cm2 (Panel B) and grown for up to 10 days without changing the media. Cell number was determined with the CCK-8 assay. A microplate reader was used to read absorbance of the media at 450 nm. Data are plotted as means ± SEM (n = 3).