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Ezh2 is not required for cardiac regeneration in neonatal mice

Fig 5

Ezh2 does not affect cell proliferation in the neonatal heart in response to injury.

(A) Immunofluorescence for phosphorylated histone H3 (red) in sections of hearts from control (Ezh2fl/fl) and mutant (Ezh2fl/fl;Myh6-Cre) mice 1 week post-resection induced at P1. (B) Quantification of phosphorylated histone H3 (pHH3)-positive cardiomyocytes. (C) Immunofluorescence for Ki67 (red) in sections of hearts from control (Ezh2fl/fl) and mutant (Ezh2fl/fl;Myh6-Cre) mice, 1 week post-resection induced at P1. (D) Quantification of Ki76-positive cardiomyocytes. (E) Immunofluorescence for phosphorylated histone 3 (red) in sections of hearts from control (Ezh2fl/fl) and mutant (Ezh2fl/fl;Myh6-Cre) mice, 1 week post-resection induced at P7. (F) Quantification of phosphorylated histone H3 (pHH3)-positive cardiomyocytes. α-actinin stained cardiomyocytes (green), and nuclei were counterstained with DAPI (blue). The top and lateral panels show Z-dimension reconstructions. Dotted lines show the mean in plots. Scale bar = 100 μm.

Fig 5

doi: https://doi.org/10.1371/journal.pone.0192238.g005