Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury
Representative photomicrographs of hematoxylin & eosin (H&E)-stained kidney sections and a semiquantitative scoring analysis of tubular injury at 24 h after intraperitoneal injection of iohexol with or without GKT137831 pretreatment.
The marked tubular injury caused by iohexol was diminished as the result of GKT137831 treatment. For semiquantitative analysis of morphological changes, 10 high-magnification (×200) fields for the cortex and the outer stipe of the outer medulla in ten mice of each group were randomly selected. Magnifications, ×400 and ×200; scale bar, 100 μm.