Oxidative stress caused by activation of NADPH oxidase 4 promotes contrast-induced acute kidney injury
Virus expressing the HA vector was used as a control. (A) Western blot analysis of the effects of HA vector-Nox4 on p38, JNK, and ERK phosphorylation. (B) Effects of inhibitors of p38 (SB203580), JNK (SP600125), and ERK (PD98059) on cell apoptosis, as measured by caspase 3/7 activity assays. (C) Levels of Nox4 protein were measured after inhibition of p38 (SB203580), JNK (SP600125), and ERK (PD98059). The data are the mean ± SD (n = 5). **p < 0.01 versus the control and ###p < 0.001 versus iohexol treatment only.