Enhanced expression of cell-surface B-cell receptor-associated protein 31 contributes to poor survival of non-small cell lung carcinoma cells
Fig 2
Identification of SP1-B7 antigen.
(A) Immunoprecipitation of SP1-B7 antigen with SP1-B7. Cell lysates from A549 adherent cells were immunoprecipitated with isotype control antibody (Con) or SP1-B7, and the immunoprecipitates were Western blotted with SP1-B7. (B) Immunoprecipitation of biotinylated A549 cell lysates with isotype control antibody, α-BAP31, and SP1-B7. Cell surface proteins of A549 adherent cells were biotinylated and subjected to immunoprecipitation. Immunoprecipitates were detected by Western blots with the indicated antibodies or SA-HRP.