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Identification and characterization of preferred DNA-binding sites for the Thermus thermophilus transcriptional regulator FadR

Fig 2

Validation of REPSA-selected FadR-binding DNA species.

Shown are LICOR images of EMSAs containing pooled DNA from either Round 1 (left lanes) or Round 5 (right lanes) of REPSA selection and increasing concentrations of FadR protein (from left to right: 0, 0.6, 6, 60, or 600 nM FadR). The electrophoretic mobility of a single protein-DNA complex (S) as well as uncomplexed ST2R24 selection template (T) and IRD7_ST2R primer (P) are indicated at right of figure.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0184796.g002