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Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells

Fig 3

Cyclic, but not static, stretch induced increase in IL-33.

Mouse IL-33 (mIL-33) expression in whole cell lysate after static (static S ~18% elongation) or cyclic stretch (cyctic S ~18% elongation, 1HZ) was detected by western blot at different time point (4h, 6h, 8h) and normalized by β-actin (right graph). ***P<0.001 compared with control (con). Each stretching group collected three wells for a single experiment, the bar graphs illustrate data representative of three independent experiments and western blot at the different time point was run separately.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0184770.g003