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Cyclic stretch induced IL-33 production through HMGB1/TLR-4 signaling pathway in murine respiratory epithelial cells

Fig 1

Cell viability during 4–8 h of cyclic stretch.

MLE-12 cells were conditioned in serum free medium for 12 h and then transferred to wells in Bioflex and underwent cyclic stretch (cyclic S) or were not stretch (con) for 4, 6 or 8 h. Viable cells were negative for Annexin-V and propidium iodide (PI) staining (Q3), early-stage apoptotic cells were positive for Annexin-V staining, but negative for PI staining (Q4), and late-stage apoptotic cells were positive for Annexin-V and PI staining (Q2).

Fig 1

doi: https://doi.org/10.1371/journal.pone.0184770.g001