Histological assessment of granulomas in natural and experimental Schistosoma mansoni infections using whole slide imaging
(A) Representative image of hepatic tissue from a mouse experimentally infected. After acquisition of whole slide images, areas with inflammatory infiltrates (marked in red) outside typical granulomas (marked in green) were measured. In (B), morphometric analyses reveal a very low incidence of infiltrates in the liver of infected wild rodent (natural infection) while mice experimentally infected show a very high proportion of infiltrates. In the small intestine, infiltrates are moderately higher in the natural compared to the acute experimental infection. Different letters indicate significant differences between the means (P < 0.0001 for all comparisons between different letters in the respective groups).