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Identification of factors involved in dimorphism and pathogenicity of Zymoseptoria tritici

Fig 3

Results of genome walking analysis of recovered T-DNA disrupted gene loci.

Predicted T-DNA insertion sites for each random mutant within putative genomic loci containing annotated genes are shown. DNA sequences obtained from genome walking PCR are illustrated as red lines aligned to a corresponding genomic locus; arrow heads determine the sequencing direction. Restriction enzymes used to obtain each of the DNA fragments prior to sequencing are indicated. Genes are named in concordance with JGI annotation by their Protein ID. The asterisks indicate the start position of the region matching the partial sequence of HPT cassette, thus pointing to the site of T-DNA integration for each mutant strain-specific locus. In case when the portion of HPT cassette was not identified, the putative genomic position of T-DNA inserted was estimated by fragment length obtained from restriction analysis and Southern Blot results. Predicted sites of T-DNA insertions are marked by vertical arrowheads. The “house-intern” RNA-Seq data of IPO323 were used to verify the current JGI models by mapping the aligned reads to the IPO323 genome sequence. The incorrectly annotated gene models obtained from JGI, as well as spliced junctions and contiguous sequences according to RNA-Seq data are indicated.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0183065.g003