Identification of factors involved in dimorphism and pathogenicity of Zymoseptoria tritici
The figure shows generated mutant strains (1.5 μl; 1×108 spores/ml) grown on water agar medium and nitrogen starvation medium (N-deprivation) after 21 days of incubation at 18°C. N-deprivation medium was used as screening medium. Water agar (H2O), which provides no external nutrition source was used for second round of screening. The wildtype strain IPO323 grows filamentously and forms mycelium on N-deprivation medium and water agar medium. The strains myco#1, myco#3, myco#20, myco#26, myco#56, myco#76, myco#77, myco#100 and myco#5552 show an extreme reduction in mycelium formation on both screening media. The myco#4 mutant grows poorly on H2O medium showing a drastically reduced mycelium formation, however being able to switch to filamentous growth on N-deprivation medium. In contrast, myco#5 displays a hyperfilamentation phenotype under tested cultivation conditions, exhibiting a severely increased filamentous growth. Scale bar corresponds to 1 cm.