Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype
Samples from wild-type and transgenic tubers were taken after 15 weeks of storage, when sprouts became visible in wild-type tubers. A) Schematic representation of a potato tuber to illustrate the sampling. A cork borer (# 2) was used to isolate the tissue approximately 5 mm below the bud indicated in red. It can be assumed that this region is enriched in vascular tissue. B) Starch content and C) sucrose content in the tissue below the growing sprout. Values represent the mean +/- SE of 5 independent tubers. Significant differences to wild type were calculated using two-tailed t-test assuming equal variances and are indicated by asterisks (**p<0.01, *p<0.05).