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Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype

Fig 5

Determination of the starch granule size.

Starch granules from freshly harvested tubers were extracted, stained with iodine and inspected under the microscope. A) representative micrographs of iodine-stained starch granules derived from tubers of wild-type and ISA-silenced lines 7, 16 and 39. B) Representative image illustrating the segmentation of images by means of the ImageJ software tool to determine the relative size of each 2D starch granule. The software allows for the manual separation of granules connected to each other in the image.

Fig 5