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Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype

Fig 4

Effect of reduced ISA gene expression on tuber yield and content of sugars.

Parameters were determined from greenhouse-grown plants at harvest A) Tuber yield was determined from 5 plants each. B) starch content, C) sucrose content and D) hexose levels were determines from tuber parenchyma. Values represent the mean +/- SE of 5 biological and 2 technical replicates Significant differences to wild type were calculated using two-tailed t-test assuming equal variances and are indicated by asterisks (***p<0.001, *p<0.05).

Fig 4

doi: https://doi.org/10.1371/journal.pone.0181444.g004