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Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype

Fig 3

Impact of reduced expression of ISA isogenes on soluble sugars and starch content in source leaves.

Metabolite contents in wild type and ISA- silenced plants were determined at the end of the light period (after 16h light) (light grey) and at the end of the dark period (after 8h darkness) (dark grey). A) starch B) sucrose, C) hexoses. Values represent the mean +/- SE of 5 biological and 2 technical replicates each.

Fig 3

doi: https://doi.org/10.1371/journal.pone.0181444.g003