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Simultaneous silencing of isoamylases ISA1, ISA2 and ISA3 by multi-target RNAi in potato tubers leads to decreased starch content and an early sprouting phenotype

Fig 2

Relative expression of isoamylase isogenes in wild type (WT) and selected transgenic lines 7, 16 and 39 as determined by qRT-PCR.

Relative expression of ISA1, ISA2 and ISA3 were determined in (A) source leaves, (B) mature potato tubers, (C) in sprouting tubers. Values were normalized to Ubiquitin and displayed relative to the expression level of the wild type which was set to one. Values displayed are the mean +/- SD of three to four independent biological samples each with two technical replicates. Significant differences to wild type were calculated using a two-tailed t-test assuming equal variances and are indicated by asterisks (***p<0.001; ** p< 0.01, *p<0.05).

Fig 2

doi: https://doi.org/10.1371/journal.pone.0181444.g002