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An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator

Fig 5

Long-term stability of L2/3 R-CaMP1.07 calcium signals.

A, Longitudinal two-photon imaging of the same group of cells in L2/3 of S1 cortex in an example mouse, at 22, 45, 65 and 100 days post-induction (DPI). For a spectrally unmixed version of the image at 65 DPI see S3 Fig. B, Two-photon images of L2/3 neurons within the same tissue volume, for which R-CaMP1.07 calcium transients were measured across days. In the imaging sessions 65 DPI and 100 DPI the very same neurons were measured (same cells as in A). For each imaging area three example ΔF/F traces with spontaneous calcium transients are shown for the cells marked in the images above. C, Pooled analysis of stability of R-CaMP1.07 calcium transients. Recordings were made in 2 mice from a total of 18, 15, 15, and 38 active cells at 22, 45, 65, and 100 DPI, respectively. Data points and box plots of peak amplitudes of calcium transient events did not show significant variation across sessions. D, Data points and box plots of decay time constants (τ) of exponentially fitted calcium transients (see inset), which also did not show a significant change across imaging sessions. E, Cumulative distribution of calcium transient amplitudes for L2/3 neurons that were measured twice at 65 and 100 DPI, respectively. A non-parametric, Wilcoxon rank sum test was used to compute P-values.

Fig 5