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An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator

Fig 2

Characterization of layer-specific R-CaMP1.07 expression in triple transgenic mice.

A, Schematic diagram of intersectional control by Cre and tTA, driven by different promoters, of the doubly regulated TITL-R-CaMP1.07 reporter line. tTA is driven by a CamK2a promoter, whereas layer-specificity is achieved by Cre expressed under the control of layer-specific promoters (p2: Rasgrf2-2A for L2/3, Nr5a1 for L4, Rbp4 for L5 and Ntsr1 for L6). B, Confocal images of coronal sections of fixed brains from the different mice, illustrating layer-specific labelling in neocortex of the respective mouse lines. C, High-resolution close-ups of the regions indicated in B, showing the R-CaMP1.07 expression patterns across cortical layers.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0179460.g002