Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation
(A, B) Knockdown of GATA4 showed a strong significant increase in Gata4 E1b mRNA transcripts in XY but not in XX gonads. WT1 knockdown resulted in a significant decrease of Gata4 E1b and Gata4 E1a transcripts in XY gonads but not in XX gonads. Transcripts were measured by qRT-PCR and normalized to Sdha . Results are shown as fold differences between cultures treated with mismatch vs. Gata4- (A) and mismatch vs. Wt1-morpholino (B). Error bars represent S.E.M. from independent biological replicates (n ≥ 5). Scale = log2. Statistical differences are indicated by asterisks: *p<0.05, **p<0.01, paired t-test.