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Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation

Fig 2

Co-expression of WT1 and GATA4 in XY and XX gonads.

(A, B) Dissected XY and XX gonads (13.5 dpc) were co-immunostained as whole-mounts using antibodies against WT1 and GATA4. Bound primary antibodies were visualized with Cy3- (GATA4) and 488 dye (WT1) conjugates. Nuclear co- localization of WT1 and GATA4 is shown in the merged images. Cell nuclei were stained with Dapi. Scale bars are 200 μm (low-power magnification) and 100 μm (high-power magnification), respectively. G = gonad. M = mesonephros.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0176296.g002