Ex vivo cultures combined with vivo-morpholino induced gene knockdown provide a system to assess the role of WT1 and GATA4 during gonad differentiation
(A, B) Dissected XY and XX gonads (13.5 dpc) were co-immunostained as whole-mounts using antibodies against WT1 and GATA4. Bound primary antibodies were visualized with Cy3- (GATA4) and 488 dye (WT1) conjugates. Nuclear co- localization of WT1 and GATA4 is shown in the merged images. Cell nuclei were stained with Dapi. Scale bars are 200 μm (low-power magnification) and 100 μm (high-power magnification), respectively. G = gonad. M = mesonephros.