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Imaging mass spectrometry identifies prognostic ganglioside species in rodent intracranial transplants of glioma and medulloblastoma

Fig 2

MALD-MSI of GM1 and GD1 in intracranial allografts of rat glioma.

(A) MSI of GM1/GD1-sialic acid (H-) d18:1 (m/z 1545) and 20:1 (m/z 1573), GD1 [M+Na-2H] d18:1 (m/z 1874) and 20:1 (m/z 1886). Intensities of the ions are represented in color based on the intensity scale provided. (B) Distribution of GM1 and NRP-1 by immunostaining with CTB and anti-NRP1 antibodies, respectively, in intracranial allografts of rat glioma. Circles indicate the position of the xenograft. Scale bar: 5 mm. GD1-sa = GD1-sialic acid.

Fig 2

doi: https://doi.org/10.1371/journal.pone.0176254.g002