Coordinated balance of Rac1 and RhoA plays key roles in determining phagocytic appetite
Fig 2
Real time activities of Rho GTPases in phagocytes upon challenge with apoptotic thymocytes.
(A) Representative images of active Rac1 and RhoA biosensor-transfected L/Stab-2 cells treated with apoptotic or fresh thymocytes (Scale bar: 20 μm). (B,C) The normalized FRET of Rac1 (B) and RhoA (C) with apoptotic and fresh thymocytes (the error bar indicates the s.e.m. for the 3 experiments). (D-G) Three regions of interest (ROIs) from the Rac1 (D) and RhoA (F) biosensor-transfected cells are shown, along with the time (Scale bar: 20 μm). The FRET of Rac1 (E) and RhoA (F) in the indicated ROIs are shown. The intensities were normalized to the value at 0 min (B,C,E,G).