Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function
Eight reverse mutant RNAs (10 nM), in which each of the eight mutations introduced in the m4 minus RNA were reverted to the original nucleotides, were incubated with the replicase (10 nM) for 0.5 h at 37°C. The replication amount was measured as described in the Materials and Methods. For comparison, the replication level of the original and m4 minus RNA are shown (dotted lines). The error bars represent standard errors (n = 3).